Biosynthesis of fosfomycin-loaded CuO nanoparticles: evaluation of antibacterial, antibiofilm properties and molecular docking analysis against biofilm-associated proteins in MDR bacteria
References
- World Health Organization. Technical consultation on in vitro diagnostics for AMR, 27–28 March 2019, WHO Headquarters, Geneva: meeting report. Geneva: World Health Organization; 2019. Report No.: WHO/MVP/EMP/IAU/2019.08.
- Mwangi J, Hao X, Lai R, Zhang ZY. Antimicrobial peptides: new hope in the war against multidrug resistance. Zool Res. 2019; 40:488. doi: 10.24272/j.issn.2095-8137.2019.062
- Neill J. Tackling drug-resistant infections globally: final report and recommendations. London: Review on antimicrobial resistance; 2016.
- Bassegoda A, Ivanova K, Ramon E, Tzanov T. Strategies to prevent the occurrence of resistance against antibiotics by using advanced materials. Appl Microbiol Biotechnol. 2018; 102:2075–89.
- Ardal C, Findlay D, Savic M, Carmeli Y, Gyssens I, Laxminarayan R, et al. Revitalizing the antibiotic pipeline: stimulating innovation while driving sustainable use and global access [Internet]. DRIVE-AB; 2018. [cited 2018 Mar 1]. Available from:
http://drive-ab.eu . - Azharuddin M, Zhu GH, Das D, Ozgur E, Uzun L, Turner AP, et al. A repertoire of biomedical applications of noble metal nanoparticles. Chem Comm. 2019; 55:6964–96.
- Canaparo R, Foglietta F, Limongi T, Serpe L. Biomedical applications of reactive oxygen species generation by metal nanoparticles. Mater. 2020; 14:53. doi: 10.3390/ma14010053
- Yao J, Yang M, Duan Y. Chemistry, biology, and medicine of fluorescent nanomaterials and related systems: new insights into biosensing, bioimaging, genomics, diagnostics, and therapy. Chem Rev. 2014; 114:6130–78.
- Devatha C. Green synthesis of nanomaterials. In: Synthesis of inorganic nanomaterials: advances and key technologies. Cambridge: Woodhead Publishing; 2018, p. 169–84.
- Padil VVT, Černík M. Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application. Inter J Nanomed. 2019; 8:889.
- Zhang Y, Tan X, Tu X, Ling F, Wang G. Efficacy and antiparasitic mechanism of curdione from Curcuma zedoaria. Aquac. 2020; 523:735186. doi: 10.1016/j.aquaculture.2020.735186
- Veisi H, Karmakar B, Tamoradi T, Hemmati S, Hekmati M, Hamelian M. Biosynthesis of CuO nanoparticles using aqueous extract of herbal tea (Stachys Lavandulifolia) flowers and evaluation of its catalytic activity. Sci Rep. 2021; 11:1–13.
- Setyani DA, Rahayu DUC, Handayani S, Sugita P. Phytochemical and antiacne investigation of Indonesian white turmeric (Curcuma zedoaria) rhizomes. In IOP Conference Series: Mater Sci Eng. 2020; 902:012066. doi: 10.1088/1757-899X/902/1/012066
- Khan BA, Ali A, Hosny KM, Halwani AA, Almehmady AM, Iqbal M, et al. Carbopol emulgel loaded with ebastine for urticaria: development, characterization, in vitro and in vivo evaluation. Drug Deliv. 2022; 29:52–61.
- Dadi R, Azouani R, Traore M, Mielcarek C, Kanaev A. Antibacterial activity of ZnO and CuO nanoparticles against gram positive and gram-negative strains. Mater Sci Eng. 2019; 104:109968. doi: 10.1016/j.msec.2019.109968
- ++ et al. Synthesis and characterization of carbon dots–coated CaCO3 nanocarrier for levofloxacin against multidrug-resistant extended-spectrum beta-lactamase Escherichia coli of urinary tract infection origin. Microb Drug Resist. 2022; 28:106–19.
- National Cancer Institute. Online SMILES translator [Internet]. Bethesda (MD): NCI; [cited 2025 Apr 19]. Available from:
https://cactus.nci.nih.gov/translate/ - Dallakyan S, Olson AJ. PyRx—Python prescription: virtual screening tool [Internet]. La Jolla (CA): The Scripps Research Institute; [cited 2025 Apr 19]. Available from:
https://pyrx.sourceforge.io/ - ChEMBL. ChEMBL database [Internet]. Cambridge (UK): European Bioinformatics Institute; [cited 2025 Apr 19]. Available from:
https://www.ebi.ac.uk/chembl/ - Rani R, Sharma D, Chaturvedi M, Yadav JP. Antibacterial activity of twenty different endophytic fungi isolated from Calotropis procera and time kill assay. Clin Microbiol. 2017; 6:280. doi: 10.4172/2327-5073.1000280
- Eid AM, Fouda A, Hassan SED, Hamza MF, Alharbi NK, Elkelish A, et al. Plant-based copper oxide nanoparticles: biosynthesis, characterization, antibacterial activity, tanning wastewater treatment, and heavy metals sorption. Catal. 2023; 13:348.
- Yang Y, Xu D, Wu Q, Diao P. Cu2O/CuO bilayered composite as a high-efficiency photocathode for photoelectrochemical hydrogen evolution reaction. Sci Rep. 2016; 6:35158. doi: 10.1038/srep35158
- Saif S, Tahir A, Asim T, Chen Y. Plant mediated green synthesis of CuO nanoparticles: comparison of toxicity of engineered and plant mediated CuO nanoparticles towards Daphnia magna. Nanomater. 2016; 6:205. doi: 10.3390/nano6110205
- Aziz WJ, Abid MA, Hussein EH. Biosynthesis of CuO nanoparticles and synergistic antibacterial activity using mint leaf extract. Mater Technol. 2020; 35:447–51.
- Mahmoud AED, Al-Qahtani KM, Alflaiji SO, Al-Qahtani SF, Alasamhan FA. Green copper oxide nanoparticles for lead, nickel, and cadmium removal from contaminated water. Sci Rep. 2021; 11:1–13.
- Asif AB, Bilal AT, Atif KW, Sheergojri GA, Kaloo MA, Bilal AB, et al. Synthesis and characterization of copper oxide nanoparticles by coprecipitation method: electronic and antimicrobial properties. Chem Sci Eng Res. 2021; 3:25–9.
- Gandhi S, Subramani RHH, Ramakrishnan T, Sivabalan A, Dhanalakshmi V, Nair MG, et al. Ultrasound assisted one pot synthesis of nano-sized CuO and its nanocomposite with poly (vinyl alcohol). J Mater Sci. 2010; 45:1688–94.
- Rohman A, Ramadhani D, Nugroho A. Analysis of curcumin in Curcuma longa and Curcuma xanthorriza using FTIR spectroscopy and chemometrics. Res J Med Plant. 2015; 9:179–86.
- Tamuly C, Saikia I, Hazarika M, Das MR. Reduction of aromatic nitro compounds catalyzed by biogenic CuO nanoparticles. RSC Adv. 2014:53229–36.
- Mohanarangan J. Balakrishnan. Synthesis and characterization of copper oxide nanoparticles as photocatalyst. Malaya J Mat. 2020; 2:4880–4.
- Patel MR, Patel RB, Parikh JR, Solanki AB, Patel BG. Effect of formulation components on the in vitro permeation of microemulsion drug delivery system of fluconazole. AAPS Pharm Sci Tech. 2009; 10:917–23.
- Uzair B, Akhtar N, Sajjad S, Bano A, Fasim F, Zafar N, et al. Targeting microbial biofilms: by Arctium lappa l. synthesised biocompatible CeO2-NPs encapsulated in nano-chitosan. IET Nanobiotechnol. 2020; 14:217–23.
- Abbas S, Uzair B, Raza A, Sajjad S, Niazi MBK. Chitosan encapsulation of fluconazole-loaded MgO/CuO nanocomposite for biofilm inhibition of contact lens. Mater Chem Phys. 2024; 317:129164. doi: 10.1016/j.matchemphys.2024.129164
- Tawre MS, Shiledar A, Satpute SK, Ahire K, Ghosh S, Pardesi K. Synergistic and antibiofilm potential of Curcuma aromatica derived silver nanoparticles in combination with antibiotics against multidrug-resistant pathogens. Front Chem. 2022; 10:1029056. doi: 10.3389/fchem.2022.1029056
- Abbasi Z, Uzair B, Khan BA, Menaa F, Saeed M, Ahmad I, et al. Tracking success of interaction of green-synthesized Carbopol nanoemulgel (neomycin-decorated Ag/ZnO nanocomposite) with wound-based MDR bacteria. Nanotechnol Rev. 2024; 13:20240027. doi: 10.1515/ntrev-2024-0027
- ChemSpider. ChemSpider: search and share chemistry [Internet]. Cambridge (UK): Royal Society of Chemistry; [cited 2025 Apr 19]. Available from:
https://www.chemspider.com/
Language: English
Page range: 21 - 34
Published on: Feb 27, 2026
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
Related subjects:
© 2026 Zukhra Abbasi, Fehmida Fasim, Sehrish Abbas, Rabia Shafique, Barkat Ali Khan, Amna Nisar, Sultan M. Alshahrani, Bushra Uzair, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.