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Development of a methodology for the volume estimation of the prefrontal cortical subfields in very pre-term infants using magnetic resonance imaging and stereology Cover

Development of a methodology for the volume estimation of the prefrontal cortical subfields in very pre-term infants using magnetic resonance imaging and stereology

Open Access
|Aug 2025

Figures & Tables

Figure 1.

Cross-sectional area point counting (points removed) for the DL PFC and OL PFC (the upper and lower left frames, respectively). Boundary length estimation for the DL PFC and OL PFC (the upper and lower right frames, respectively). The stereological grids appear as red crosses in the cross-sectional area point counting, and as red lines in the boundary length estimation. The yellow crosses indicated demonstrate the intersections between the structure boundaries and the grid. DL, dorsolateral; OL, orbitolateral; PFC, prefrontal cortex.
Cross-sectional area point counting (points removed) for the DL PFC and OL PFC (the upper and lower left frames, respectively). Boundary length estimation for the DL PFC and OL PFC (the upper and lower right frames, respectively). The stereological grids appear as red crosses in the cross-sectional area point counting, and as red lines in the boundary length estimation. The yellow crosses indicated demonstrate the intersections between the structure boundaries and the grid. DL, dorsolateral; OL, orbitolateral; PFC, prefrontal cortex.

The calculated shape coefficient for PF subcortical fields

RegionShape coefficient
DL4.5
DM6.1
OL6.4
OM6.5

Clinical characteristics of preterm infants

Infant IDGestational age (weeks)Birth weight (g)Reason for preterm deliveryComorbidities
0125 + 6870PreeclampsiaBronchopulmonary dysplasia
0226 + 2920PROMNone
0327 + 1980Spontaneous preterm laborNone
0426 + 0860Spontaneous preterm laborHyperbilirubinemia
0525 + 5910PreeclampsiaBronchopulmonary dysplasia

The calculated CE for the estimated volume of the DLPFC subfield

Grid size (pixels) Slice gap (cm)
0.10.20.3
2CE (%)2.1764.1456.46
V (mm3)8.4288.1528.316
3CE (%)2.2314.2546.546
V (mm3)8.4248.1548.343
4CE (%)2.3484.3826.717
V (mm3)8.4808.1928.352
5CE (%)2.7294.8997.14
V (mm3)8.1757.9008.250

Recommended stereological parameters

PFC subfieldGrid size (pixels)Slice gap (cm)Shape coefficient √B/√√ACE (%)
DL4 × 40.24.54.38
DM5 × 50.16.14.28
OL5 × 50.36.43.92
OM5 × 50.16.53.86

The calculated CE for the estimated volume of the DMPFC subfield

Grid size (pixels) Slice gap (cm)
0.10.20.3
2CE (%)4.058.46812.161
V (mm3)7.2768.0168.496
3CE (%)4.0978.49912.264
V (mm3)7.2908.0288.505
4CE (%)4.2018.62712.384
V (mm3)7.2808.0328.496
5CE (%)4.2828.69812.249
V (mm3)7.3258.0508.550

The calculated CE for the estimated volume of the OLPFC subfield

Grid size (pixels) Slice gap (cm)
0.10.20.3
2CE (%)1.9924.2566.078
V (mm3)8.5928.2248.208
3CE (%)2.1191.1361.402
V (mm3)8.5778.1908.208
4CE (%)1.1881.7342.15
V (mm3)8.6408.2888.256
5CE (%)1.6592.422.986
V (mm3)8.6508.3008.325
6CE (%)2.1833.1883.928
V (mm3)8.6408.2808.316
7CE (%)2.7323.9994.922
V (mm3)8.7228.3308.379

The calculated CE for the estimated volume of the OMPFC subfield

Grid size (pixels) Slice gap (cm)
0.10.20.3
2CE (%)3.2276.367.946
V (mm3)6.5447.0647.464
3CE (%)3.4494.9448.047
V (mm3)6.8317.6147.479
4CE (%)3.3936.4938.071
V (mm3)6.5767.0727.488
5CE (%)3.6886.8418.567
V (mm3)6.5507.1007.500
6CE (%)3.8666.8998.639
V (mm3)6.5167.0567.344
7CE (%)4.2437.388.999
V (mm3)6.4686.9587.350
DOI: https://doi.org/10.2478/abm-2025-0023 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Language: English
Page range: 174 - 182
Published on: Aug 31, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year

© 2025 Faten Aldhafeeri, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.