Have a personal or library account? Click to login
Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China Cover

Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China

Asian Biomedicine
Volume 18 (2024): Issue 1 (February 2024)
By: Qing-Qing Jiang,  Juan-Juan Zhu,  Shu-Ling Fan,  Ya-Ping Hou,  Xie-Ying Hu,  Jie Shi,  Lei Wu and  Ying Luo  
Open Access
|Mar 2024

Figures & Tables

Figure 1.

Schematic representation and results of hearing loss gene chip. (A) The schematic representation of the location of the probes dotted in the hearing loss gene chip used for the reverse dot blot assay. N represented the location of wild-type probes. M represented the location of mutant probes. (B) Part of the hybridization results of hearing loss gene chip, which included heterozygous, and homozygous gene mutations. (-) presented no probe at the corresponding position in the hybridization membrane.

Figure 2.

Detection of deafness gene mutations in 213 volunteers.

The information on primers in the reaction system

NameSequence (5′→3′)
GJB2-F(Biotin)-TAGTGATTCCTGTGTTGTGTG
GJB2-RTTCTGGGTTTTTGATCTCCTC
GJB3-F(Biotin)-CTCTTCCTCTACCTGCTGC
GJB3-RTATTGCCTGGGTCTGGAT
SLC26A4-F1(Biotin)-TTCACTGCTGGATTGCTCA
SLC26A4-R1GTGTTAACCGTACATGTTCTGC
SLC26A4-F2(Biotin)-CTCTCAGATGGTATGGCGTC
SLC26A4-R2TCCTTCATTACTGATTCCTTGTC
SLC26A4-F3(Biotin)-GTTCTTTGACGACAACATTAG
SLC26A3-R3AATGGAACCTTGACCCTCTT
mtDNA-F1(Biotin)-ATGAGGTGGCAAGAAATG
mtDNA-R1TTGGCTAAGGTTGTCTGGTA
mtDNA-F2(Biotin)-GATGCATACACCACATGAAAC
mtDNA-R2TGAGTGTTAGGAAAAGGGCA
mtDNA-F3(Biotin)-CACAATGGGGCTCACTCA
mtDNA-R3ACGAACAATGCTACAGGGAT

The information of probes in the hearing loss gene chip

NameSequence (5′→3′)
1494-PNCGTCACCCTCCTCAAG
1494-PMCCGTCACTCTCCTCAAG
1555 PNAGAGGAGACAAGTCGTAAC
1555 PMGAGGAGGCAAGTCGTAA
7445 PNACATAAAATCTAGA-CAAAAAAGGA
7445 PMCATAAAATCTAGGCAAAAAAGG
12201 PNGACCCCTTATTTACCGAGAA
12201 PMCCCCTTACTTACCGAGA
G2-35 PNGTTCACACCCCCCAGGA
G2-35 PMAATGGAACCTTGACCCTCTT
G2-155 PNGGTGTTGCAGACAAAGTCG
G2-155 PMCGACTTTGCAACACCC
G2-176 PNGCACACGTTCTTGCAGCC
G2-176 PMCAGCCAGCTACGATCAC
G2-235 PNAGCTGCAGGGCCCATA
G2-235 PMTATGGGCCTGCAGCT
G2-299 PNTTCTTCTCATGTCTCCGGT
G2-299 PMACCGGAGACGAGAAGAA
G3-538 PNACATTGCCCGACCTAC
G3-538 PMACATTGCCTGACCTACC
IVS7-2 PNGTTTTATTTCAGACGATAATTGC
IVS7-2 PMTGTTTTATTTCGGACGATAAT
1229 PNCTGCTCTTTCCCGCACGG
1229 PMCTCTTTCCCGCATGGCC
2168 PNCGGTCCATGATGCTATA
2168 PMGTCCGTGATGCTATACT

The genotypes of nine individuals with hearing loss gene mutation

GJB2nGJB3nSLC26A4nmtDNAn
c.35delG0c.538C>T0IVS7-2A>G2m.1494C>T0
c.155_158del40 c.1229C>T1m.1555A>G1
c.176_191del160 c.2168A>G0m.7445A>G0
c.235delC4 m.12201T>C0
c.299_300delAT1
DOI: https://doi.org/10.2478/abm-2024-0003 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Journal RSS Feed
Language: English
Page range: 11 - 17
Published on: Mar 20, 2024
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
hearing-loss gene,
reverse dot blot assay
Related subjects:
Medicine,
Assistive professions, nursing,
Basic medical science,
Basic medical science, other,
Clinical medicine,
Clinical medicine, other

© 2024 Qing-Qing Jiang, Juan-Juan Zhu, Shu-Ling Fan, Ya-Ping Hou, Xie-Ying Hu, Jie Shi, Lei Wu, Ying Luo, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 18 (2024): Issue 1 (February 2024)Next article