Figure 1.
Schematic representation and results of hearing loss gene chip. (A) The schematic representation of the location of the probes dotted in the hearing loss gene chip used for the reverse dot blot assay. N represented the location of wild-type probes. M represented the location of mutant probes. (B) Part of the hybridization results of hearing loss gene chip, which included heterozygous, and homozygous gene mutations. (-) presented no probe at the corresponding position in the hybridization membrane.
Table 1.
The information on primers in the reaction system
| Name | Sequence (5′→3′) |
|---|---|
| GJB2-F | (Biotin)-TAGTGATTCCTGTGTTGTGTG |
| GJB2-R | TTCTGGGTTTTTGATCTCCTC |
| GJB3-F | (Biotin)-CTCTTCCTCTACCTGCTGC |
| GJB3-R | TATTGCCTGGGTCTGGAT |
| SLC26A4-F1 | (Biotin)-TTCACTGCTGGATTGCTCA |
| SLC26A4-R1 | GTGTTAACCGTACATGTTCTGC |
| SLC26A4-F2 | (Biotin)-CTCTCAGATGGTATGGCGTC |
| SLC26A4-R2 | TCCTTCATTACTGATTCCTTGTC |
| SLC26A4-F3 | (Biotin)-GTTCTTTGACGACAACATTAG |
| SLC26A3-R3 | AATGGAACCTTGACCCTCTT |
| mtDNA-F1 | (Biotin)-ATGAGGTGGCAAGAAATG |
| mtDNA-R1 | TTGGCTAAGGTTGTCTGGTA |
| mtDNA-F2 | (Biotin)-GATGCATACACCACATGAAAC |
| mtDNA-R2 | TGAGTGTTAGGAAAAGGGCA |
| mtDNA-F3 | (Biotin)-CACAATGGGGCTCACTCA |
| mtDNA-R3 | ACGAACAATGCTACAGGGAT |
Table 2.
The information of probes in the hearing loss gene chip
| Name | Sequence (5′→3′) |
|---|---|
| 1494-PN | CGTCACCCTCCTCAAG |
| 1494-PM | CCGTCACTCTCCTCAAG |
| 1555 PN | AGAGGAGACAAGTCGTAAC |
| 1555 PM | GAGGAGGCAAGTCGTAA |
| 7445 PN | ACATAAAATCTAGA-CAAAAAAGGA |
| 7445 PM | CATAAAATCTAGGCAAAAAAGG |
| 12201 PN | GACCCCTTATTTACCGAGAA |
| 12201 PM | CCCCTTACTTACCGAGA |
| G2-35 PN | GTTCACACCCCCCAGGA |
| G2-35 PM | AATGGAACCTTGACCCTCTT |
| G2-155 PN | GGTGTTGCAGACAAAGTCG |
| G2-155 PM | CGACTTTGCAACACCC |
| G2-176 PN | GCACACGTTCTTGCAGCC |
| G2-176 PM | CAGCCAGCTACGATCAC |
| G2-235 PN | AGCTGCAGGGCCCATA |
| G2-235 PM | TATGGGCCTGCAGCT |
| G2-299 PN | TTCTTCTCATGTCTCCGGT |
| G2-299 PM | ACCGGAGACGAGAAGAA |
| G3-538 PN | ACATTGCCCGACCTAC |
| G3-538 PM | ACATTGCCTGACCTACC |
| IVS7-2 PN | GTTTTATTTCAGACGATAATTGC |
| IVS7-2 PM | TGTTTTATTTCGGACGATAAT |
| 1229 PN | CTGCTCTTTCCCGCACGG |
| 1229 PM | CTCTTTCCCGCATGGCC |
| 2168 PN | CGGTCCATGATGCTATA |
| 2168 PM | GTCCGTGATGCTATACT |
Figure 2.
Detection of deafness gene mutations in 213 volunteers.