Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China

Qing-Qing Jiang; Juan-Juan Zhu; Shu-Ling Fan; Ya-Ping Hou; Xie-Ying Hu; Jie Shi; Lei Wu; Ying Luo

doi:10.2478/abm-2024-0003

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Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China

Asian Biomedicine

Volume 18 (2024): Issue 1 (February 2024)

By: Qing-Qing Jiang, Juan-Juan Zhu, Shu-Ling Fan, Ya-Ping Hou, Xie-Ying Hu, Jie Shi, Lei Wu and Ying Luo

Open Access

|Mar 2024

Abstract

Background

Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.

Objective

Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.

Methods

A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss–related genes including GJB2, GJB3, SLC26A4, and the mitochondrial gene MT-RNR1. This method involved PCR amplification systems and a hybridization platform.

Results

The technique can detect 13 hotspot mutations of 4 hearing loss–related genes. And a total of 213 blood samples were used to evaluate the availability of this method.

Discussion

Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss–related genes in a Chinese population.

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Articles in this issue

DOI: https://doi.org/10.2478/abm-2024-0003 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415

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Language: English

Page range: 11 - 17

Published on: Mar 20, 2024

Published by: Chulalongkorn University

In partnership with: Paradigm Publishing Services

Publication frequency: 6 issues per year

Keywords:

GJB2,

GJB3,

hearing-loss gene,

MT-RNR1,

reverse dot blot assay,

SLC26A4

Related subjects:

Medicine,

Assistive professions, nursing,

Basic medical science,

Basic medical science, other,

Clinical medicine,

Clinical medicine, other

© 2024 Qing-Qing Jiang, Juan-Juan Zhu, Shu-Ling Fan, Ya-Ping Hou, Xie-Ying Hu, Jie Shi, Lei Wu, Ying Luo, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 18 (2024): Issue 1 (February 2024)