Fig. 1.
Primers used in this study_
| Target gene | Oligonucleotide sequences (5’-3’) | Size of product (bp) | Reference |
|---|---|---|---|
| van A | GGGAAAACGACAATTGC GTACAATGCGGCCGTTA | 732 | Dutka-Malen et al. 1995 |
| vanB | ATG GGA AGC CGA TAG TC GAT TTC GTT CCT CGA CC | 638 | Dutka-Malen et al. 1995 |
| vanC | AAT CGT CAA TTC CTG CAT GT TAA TCG TGG AAT ACG GGT TTG | 299 | Dutka-Malen et al. 1995 |
| aac(6’)-Ie aph(2’’) | AGGAATTTATCGAAAATGGTAGAAAAG CACAATCGACTAAAGAGTACCAATC | 369 | Vakulenko et al. 2003 |
| aph(3’)-IIIa | GGCTAAAATGAGAATATCACCGG CTTTAAAAAATCATACAGCTCGCG | 523 | Vakulenko et al. 2003 |
| ant(4’)-Ia | CAAACTGCTAAATCGGTAGAAGCC GGAAAGTTGACCAGACATTACGAACT | 294 | Vakulenko et al. 2003 |
Frequency of vancomycin and gentamicin resistance genes among our local Enterococci isolates_
| Genus | van gene | Gentamicin gene | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| vanA | vanB | vanA, vanB | vanC | 3’ | 4’ | 6’ | 3’ + 4’ | 3’ + 6’ | 4’ + 6’ | 3’ + 4’ + 6’ | |
| E. faecalis | 14 | 3 | 3 | 1 | 74 | 8 | 67 | 3 | 26 | 6 | 4 |
| E. faecium | 9 | 0 | 3 | 2 | 48 | 10 | 42 | 6 | 50 | 2 | 1 |
| Total (n) | 23 | 3 | 6 | 3 | 122 | 18 | 109 | 9 | 76 | 8 | 5 |
The association between presence of gentamicin resistance genes and MICs against gentamicin_
| Gene target | MIC (µg/ml) | ||||||
|---|---|---|---|---|---|---|---|
| 256 | 512 | 1024 | |||||
| E. faecalis | E. faecium | E. faecalis | E. faecium | E. faecalis | E. faecium | ||
| Gentamicin resistance gene | aph(3’)-IIIa | 0 | 0 | 3 | 1 | 2 | 3 |
| ant(4’)-Ia | 0 | 0 | 3 | 1 | 0 | 0 | |
| aac(6’)-Ie aph(2’’) | 0 | 0 | 5 | 2 | 2 | 2 | |