Bacterial Microbiota and Fatty Acids in the Faeces of Overweight and Obese Children

The sequence of oligonucleotide probes and hybridization conditions used in FISH procedure for the identification of the bacteria present in children faeces.

Probe	Identified microorganisms	Sequence (5’→3’)	Fluorescent label	Temp [°C]	Time [h]
Lab 158	Lactobacillus-Enterococcus	GGT ATT AGC A(T?C)CTGT TTC CA	5’Fluo	46	24
Bif 164	Bifidobacterium spp.	CAT CCG GCA TTA CCA CCC	5’Cy3	58	18
Bac 303	Bacteroides-Prevotella	CCA ATG TGG GGG ACC TT	5’Cy3	55	3
Erec 484	Clostridium coccoides	GCT TCT TAG TCA GGT ACC G	5’Cy3	57	16
Prov	Prevotella	ATCTTGAGTGAGTTCGATGTTGG	5’Fluo	57	18

The number of bacteria isolated from stool of overweight, obese and normal weight children

Contribution of the main types of bacteria isolated from the stool of obese children (a), normal weight children (b), and obese 3 children at > 95 percentile (c)

Table II

SCFAs and BCFAs in the stool of overweight, obese and normal weight children.

Acid	Obese children			Children with normal weight
Acid	Acid concentration [mg g^–1 stool]	Average [mg g^–1 stool]	Median [mg g^–1 stool]	Acid concentration [mg g^–1 stool]	Average [mg g^–1 stool]	Median [mg g^–1 stool]	p
Lactic	0.017–4.351	1.35	1.126	0.093–4.909	2.24	1.804	0.014
Acetic	0.111–1.289	0.71	0.650	0.026–3.269	1.38	1.122	0.279
Propionic	0.085–1.232	0.67	0.594	0.050–2.128	1.08	0.985	0.354
Butyric	0.014–0.543	0.40	0.381	0.030–0.948	0.33	0.299	0.446
Formic	0.008–0.660	0.20	0.199	0.010–0.484	0.21	0.154	0.645
Valeric	0.012–0.412	0.26	0.254	0.063–0.472	0.20	0.187	0.164
Total SCFA	0.342–7.521	3.59	2.708	0.424–10.18	5.44	3.974	0.040
BCFA
Isobutyric	0.048–0.341	0.16	0.100	0.020–0.545	0.23	0.200	0.640
Isovalerian	0.017–0.528	0.20	0.120	0.001–1.180	0.20	0.121	0.913
Total BCFA	0.022–0.896	0.36	0.255	0.001–1.151	0.44	0.421	0.741

1 Analysis was based on U Mann-Whitney test. Statistical significance was established at p < 0.05.

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DOI: https://doi.org/10.21307/pjm-2018-041 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 339 - 345

Submitted on: Dec 28, 2017

Accepted on: Jun 3, 2018

Published on: Sep 4, 2018

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Actinobacteria,

Bacteroidetes,

Firmicutes,

BCFAs,

obesity,

SCFAs

Related subjects:

Life sciences,

Microbiology and virology

© 2018 RENATA BARCZYŃSKA, MIECZYSŁAW LITWIN, KATARZYNA SLIŻEWSKA, MIECZYSLAW SZALECKI, AGNIESZKA BERDOWSKA, KATARZYNA BANDURSKA, ZDZISŁAWA LIBUDZISZ, JANUSZ KAPUŚNIAK, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 67 (2018): Issue 3 (September 2018)

Bacterial Microbiota and Fatty Acids in the Faeces of Overweight and Obese Children

Figures & Tables

Table I

Fig. 1.

Fig. 2.

Table II

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