Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Composition of the Protein Deglycosylation Mix_
| Enzyme | Hydrolyzed bounds | Number of enzymatic units added per 100 μl of reaction |
|---|---|---|
| O-Glycosidase | Catalyzes the removal of core 1 and core 3 O-linked disaccharides from glycoproteins. | 400000 U |
| PNGase F | Cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins unless α(1-3) core fucosylated | 5000 U |
| Neuraminidase | Catalyzes the hydrolysis of terminal, non-reducing α2,3, α2,6, and α2,8 linked N-acetylneuraminic acid residues from glycoproteins and oligosaccharides. | 500 U |
| β1-4 Galactosidase | Catalyzes the hydrolysis of terminal, non-reducing β1-4 linked D-galactopyranosyl residues from oligosaccharides and glycoproteins. | 80 U |
| β-N-Acetylglucosaminidase | Catalyzes the hydrolysis of terminal, non-reducing β-N-Acetylglucosamine residues from oligosaccharides and glycoproteins. | 40 U |
Function of glycosidase inhibitors used to inhibit EPS synthesis in L_ casei Fyos_
| GT inhibitor | The enzyme inhibited |
|---|---|
| Castanospermine | β-glucosidase |
| Deoxymannojirimycin | α-(1→2)-mannosidase |
| Kifunensine | |
| Swainsonine | α-(1→3)-mannosidase |
| Deoxynojirimycin hydrochloride | α-glucosidase |
Monosaccharides composition of the EPS produced by L_ rhamnosus KL 53A and L_ casei Fyos when grown on MRS agar (anaerobic conditions, 37°C, 18 h)_
| Monomer | % of a monomer in EPS isolated from the strain tested | |
|---|---|---|
| Lactobacillus rhamnosus KL 53A | Lactobacillus casei Fyos | |
| Maltose | 0.71 | 0 |
| Glucose | 30.9 | 15.7 |
| Galactose | 53.7 | 31.4 |
| Arabinose | 14.6 | 51.6 |
| Ribose | 0 | 1.4 |