Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

List of isolates originating from the different habitats identified as the Trichoderma species and analyzed for their cellulolytic and xylanolytic activity.

Culture code	Origin		NCBI GenBank Assession No.		Cellulolytic activity (the plate method)	Xylanolytic activity (the plate method)
Culture code	Localization	Source	ITS	tef1	Cellulolytic activity (the plate method)	Xylanolytic activity (the plate method)
*T. reesei*
QM9414					1.00	1.00
T. viride (A)
AN109^{^*}, AN176^¹ AN141 AN142 AN179 AN 235	Central Poland	Forest wood^²	HQ292923	HQ293010	0.45/0.50	0.70/0.35
			HQ292922	HQ293008	0.75 ± 0.09	1.75 ± 0.27^{^a}
			HQ292920	HQ293009	2.50 ± 0.31^{^a}	0.67 ± 0.15
			HQ292924	HQ293011	0.56 ± 0.15	0.55 ± 0.18
		Forest soil	HQ292921	HQ293013	0.45 ± 0.15	0.75 ± 0.12
AN242 AN244, AN249^¹ AN250, AN255^¹ AN371 AN401	Southern Poland	Forest wood	JX184121	JX184098	0.55 ± 0.18	0.58 ± 0.15
			JX184122	JX184099	0.46/1.40	0.75/1.42
			JX184121	JX184098	1.03/0.78	0.77/0.81
			JX184124	JX184100	0.91 ± 0.17	0.92 ± 0.09
			JX184121	JX184099	0.75 ± 0.14	0.47 ± 0.21
T. iridescent complex (B)
AN93 AN122 AN145 AN148 AN149	Central Poland	Forest wood	HQ292927	HQ292995	0.85 ± 0.21	0.73 ± 0.18
			HQ292928	HQ292994	0.79 ± 0.12	0.57 ± 0.19
			HQ292930	HQ292996	0.85 ± 0.17	0.3 ± 0.33
			HQ292933	HQ292999	0.79 ± 0.23	0.44 ± 0.11
			HQ292934	HQ293000	0.67 ± 0.20	0.48 ± 0.14
AN158^{^*}	Eastern Poland	Wheat kernels	JX184127	JX184103	0.55 ± 0.12	0.76 ± 0.19
AN227 AN229 AN231	Central Poland	Forest wood	HQ292936	HQ293001	0.67 ± 0.21	0.57 ± 0.13
			HQ292937	HQ293002	0.83 ± 0.16	0.19 ± 0.08
			HQ292938	HQ293003	0.50 ± 0.23	0.58 ± 0.13
AN245 AN248 AN323, AN334, AN405^¹	Southern Poland	Forest wood	JX184127	JX184103	0.50 ± 0.10	0.56 ± 0.16
			JX184128	JX184104	0.63 ± 0.15	0.75 ± 0.23
			JX184127	JX184103	0.78/0.85/0.10	0.58/0.58/0.55
T. vixens (C)
AN68 AN69 AN70 AN73 AN74 AN75 AN160 AN185	Eastern Poland	Garden compost	HQ292943	–	2.09 ± 0.27^{^a}	1.10 ± 0.11
			HQ292944	–	1.60 ± 0.19	3.00 ± 0.27^{^a}
			HQ292947	–	2.10 ± 0.22^{^a}	1.10 ± 0.15
			HQ292945	–	2.75 ± 0.31^{^a}	1.10 ± 0.18
			HQ292946	–	1.25 ± 0.12	1.40 ± 0.17
			HQ292948	–	1.53 ± 0.15	1.10 ± 0.13
		Grass root	HQ292945	–	0.83 ± 0.18	0.55 ± 0.18
	Central Poland	Mushroom compost^³	HQ292947	–	0.95 ± 0.12	0.55 ± 0.12
AN186^{^}, AN187^{^}, AN188^{^} AN204^{^}^¹	Eastern Poland		HQ292946	–	0.8/2.6/2.0	1.00/2.25^{^a}/1.15
AN186^{^}, AN187^{^}, AN188^{^} AN204^{^}^¹	Eastern Poland		HQ292948	–	1.35 ± 0.21	1.45 ± 0.17
AN267 – ITEM 1357 AN268 – ITEM 1591 AN269 – ITEM 1594	Bari. Italy		– – –	– – –	1.25 ± 0.17 2.50 ± 0.23^{^a} 1.6 ± 0.19	0.55 ± 0.20 2.70 ± 0.32^{^a} 1.10 ± 0.12
T. harzianum complex (D)
AN91	Central Poland	graden Kompost	HQ292860	–	0.75 ± 0.15	1.15 ± 0.11
AN94		forest soil	HQ292873	–	0.80 ± 0.12	3.55 ± 0.27^{^a}
AN101 AN108/AN110^{^*}		forest wood^²	HQ292868	–	1.60 ± 0.21	2.58 ± 0.23^{^a}
AN101 AN108/AN110^{^*}		forest wood^²	HQ292869	–	2.80^{^a}/1.10	1.80^{^a}/1.40
AN131^{^} AN132 AN133 AN134 AN135 AN136 AN137 AN138 AN150 AN177^{^} AN181	Central Poland	forest wood^²	HQ292870	–	1.10 ± 0.16	0.60 ± 0.09
			HQ292867	–	0.78 ± 0.12	0.60 ± 0.07
			HQ292874	–	2.05 ± 0.26^{^a}	1.70 ± 0.21^{^a}
			HQ292875	–	1.01 ± 0.11	1.00 ± 0.17
			HQ292876	–	0.81 ± 0.13	1.65 ± 0.14
			HQ292901	–	2.40 ± 0.22^{^a}	2.04 ± 0.18^{^a}
			HQ292877	–	1.60 ± 0.13	1.03 ± 0.12
			HQ292861	–	1.00 ± 0.10	1.41 ± 0.16
			HQ292878	–	0.87 ± 0.08	1.67 ± 0.16^{^a}
			HQ292883	–	1.25 ± 0.15	1.40 ± 0.11
			HQ292875	–	1.66 ± 0.12^{^a}	2.25 ± 0.22^{^a}
AN203 AN205 AN207 AN211		mushroom compost^³	HQ292879	–	1.66 ± 0.13^{^a}	1.00 ± 0.18
			HQ292880	–	1.36 ± 0.17	2.45 ± 0.25^{^a}
			HQ292881	–	1.50 ± 0.13	1.00 ± 0.17
			HQ292882	–	1.10 ± 0.11	0.20 ± 0.11
AN223		Forest soil	HQ292902	–	1.25 ± 0.10	1.05 ± 0.20
AN258 AN260		Forest wood	HQ292271	–	0.80 ± 0.08	1.41 ± 0.26
AN258 AN260			HQ292884		0.89 ± 0.11	1.20 ± 0.19
AN273 AN275 AN276 AN278 AN282 AN283 AN284 AN285 AN286			HQ292886	–	1.37 ± 0.19	1.45 ± 0.15
			HQ292888	–	1.20 ± 0.12	1.40 ± 0.11
			HQ292889	–	0.80 ± 0.09	1.05 ± 0.07
			HQ292890	–	0.75 ± 0.10	1.25 ± 0.19
			HQ292891	–	0.30 ± 0.07	1.10 ± 0.12
			HQ292892	–	0.85 ± 0.15	1.27 ± 0.27
			HQ292893	–	1.15 ± 0.20	1.27 ± 0.22
			HQ292894		1.10 ± 0.18	1.28 ± 0.11
			HQ292895	–	1.35 ± 0.29	1.30 ± 0.13
AN349 AN360, AN367, AN373, AN3811	Southern Poland	Forest wood	JX184111	JX184089	1.05 ± 0.09	1.38 ± 0.24
AN349 AN360, AN367, AN373, AN3811	Southern Poland	Forest wood	JX184113	JX184090	2.20^{^a}/1.70^{^a}/0.70 1.46	1.27/1.40/1.75^{^a} 1.52
AN220 – ITEM 1328	Bari, Italy		–	–	0.25 ± 0.16	1.60 ± 0.13
T. hamatum (E)
AN118	Central Poland	Forest wood^²	HQ292854	–	0.66 ± 0.21	0.61 ± 0.19
AN155	Eastern Poland	Ryder Rhizosphäre	HQ292851	–	0.82 ± 0.18	0.51 ± 0.11
AN175 AN225, AN238	Central Poland	Forest wood	HQ292854	–	0.53 ± 0.12	0.28 ± 0.10
		Forest soil	HQ292856	–	0.95 ± 0.08	0.75 ± 0.11
		Forest soil	HQ292853		0.41 ± 0.21	0.84 ± 0.29
AN277, AN279, AN501^{^*}^¹		Forest wood	HQ292853	–	0.50/0.18/0.41	2.3/0.72/0.61
AN521	Northern Poland	Forest wood	HQ292856	–	1.05 ± 0.24	0.75 ± 0.15
T. atroviride (F)
AN19 AN35 AN90 AN95 AN96 AN111	Central Poland	Forest soil	HQ292786	HQ292963	0.67 ± 0.13	1.04 ± 0.16
		Maize kernels	HQ292787	HQ292953	0.35 ± 0.09	0.80 ± 0.19
		Garden soil	HQ292788	HQ292954	0.49 ± 0.12	0.35 ± 0.12
		Garden compost	HQ292789	HQ292955	0.90 ± 0.17	1.30 ± 0.18
		Garden compost	HQ292790	HQ292956	0.95 ± 0.15	0.70 ± 0.14
		Forest wood	HQ292791	HQ292964	0.37 ± 0.13	0.75 ± 0.21
AN152 AN153 AN182 AN206 AN212 AN215	Central Poland	Triticale kernel	HQ292792	HQ292957	0.59 ± 0.19	1.85 ± 0.30^{^a}
		Triticale kernel	HQ292793	HQ292958	0.58 ± 0.10	0.57 ± 0.12
		Forest wood	HQ292794	HQ292965	0.57 ± 0.12	0.45 ± 0.23
		Mushroom compost	HQ292804	HQ292960	0.40 ± 0.19	1.02 ± 0.11
			HQ292795	HQ292966	0.68 ± 0.21	1.27 ± 0.21
			HQ292796	HQ292967	1.10 ± 0.16	1.19 ± 0.14
AN224 AN240	Southern Poland	Forest wood	HQ292799	HQ292970	0.36 ± 0.09	0.58 ± 0.13
AN224 AN240	Southern Poland	Forest wood	JX184119	JX184096	0.23 ± 0.19	0.57 ± 0.17
AN287	Central Poland		HQ292798	HQ292969	0.41 ± 0.11	0.58 ± 0.12
T. longibrachiatum (G)
AN197	Eastern Poland	Mushroom factory	HQ292780	–	0.77 ± 0.19	1.40 ± 022
AN213	Central Poland	Mushroom compost^³	HQ292781	–	0.50 ± 0.16	2.70 ± 0.31^{^a}
T. citrinoviride (H)
AN89 AN98 AN198 AN199 AN201	Central Poland	Garden soil	HQ292841	–	0.50 ± 0.14	1.25 ± 0.27
		Forest wood	HQ292843	–	1.55 ± 0.13	1.07 ± 0.19
		Mushroom factory	HQ292845		0.57 ± 0.17	0.47 ± 0.13
			HQ292846	–	0.95 ± 0.12	2.07 ± 0.26^{^a}
			HQ292849	–	1.10 ± 0.21	2.08 ± 0.29^{^a}
AN262, AN303, AN393, AN500^¹	Southern Poland	Forest wood	JX184109	–	2.26^{^a}/0.85/1.75^{^a} 0.90	2.88^{^a}/0.61/0.63 0.80
T. pseudokoningii (I)
AN219 – ITEM1416	Bari. Italy		–	–	1.42 ± 0.19	2.10 ± 0.22^{^a}
T. koningii (J)
AN100 AN105 AN106 AN121 AN128 AN151	Central Poland	Forest wood	HQ292903	HQ292975	0.35 ± 0.17	0.40 ± 0.17
			HQ292905	HQ292977	0.17 ± 0.08	0.58 ± 0.14
			HQ292906	HQ292978	1.16 ± 0.19	0.40 ± 0.16
			HQ292913	HQ292985	0.58 ± 0.11	1.41 ± 0.27
			HQ292918	HQ292989	0.31 ± 0.09	0.48 ± 0.12
			HQ292919	HQ292990	0.47 ± 0.11	0.55 ± 0.12
T. koningiopsis (K)
AN222 – ITEM 2688	Bari, Italy		–	–	0.20 ± 0.10	0.30 ± 0.12

1* The isolates identified in this study by a combination of morphological and molecular analyses

2a – Indicate the statistically significant higher values in comparison to the reference strain T. reesei (QM 9414) at P ≤ 0.05

31 – The identical accession numbers refer to identical sequences

42 – The pieces of decaying wood collected from the floor of forests/parks

53 – The compost used for Agaricus bisporus cultivation

6 A. B. C. … – corresponds to particular species presented in fig. 1.

Cellulolytic (part A) and xylanolytic activity (part B) of the studied fungal strains relative to the reference strain *T. reesei* QM9414 – the plate method analysis. The studied fungal strains belonged to the following species: A – *T. viride*, B – *T. viridescens*, C – *T. virens*, D – *T. harzianum*, E – *T. hamatum*, F – *T. atroviride*, G – *T. longibrachiatum*, H – *T. citrinoviride*, I – *T. pseudokoningii*, J –*T. koningii*.
* The value corresponding to difference in clearing zone diameter between analyzed strains

Table II

Total cellulase activities of selected Trichoderma strains measured by the filter paper assay method (FPA).

Cellulolytic activity (FPU/ml)	Strains of Trichoderma
	T. reesei	T. virens						T. harzianum
	QM 9414	AN 68	AN 69	AN 73	AN 187	AN 188	AN 268	AN 94	AN 108
	2.11 ± 0.25	4.41 ± 0.31	3.21 ± 0.15	6.05 ± 0.46	5.62 ± 0.41	4.42 ± 0.39	6.36 ± 0.48	1.69 ± 0.09	7.15 ± 0.30

Cellulolytic activity (FPU/ml)	Strains of Trichoderma
	T. harzianum						T. longibrachiatum	T. citrinoviride
	AN 133	AN 136	AN 181	AN 203	AN 360	AN 367	AN 213	AN 262
	5.31 ± 0.28	6.13 ± 0.55	4.11 ± 0.16	3.33 ± 0.22	5.60 ± 0.25	3.70 ± 0.23	1.01 ± 0.21	4.70 ± 0.34

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DOI: https://doi.org/10.21307/pjm-2018-021 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 181 - 190

Submitted on: May 22, 2017

Accepted on: Feb 2, 2018

Published on: Jun 30, 2018

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

microorganisms screening,

Trichoderma species,

lignocellulose biomass,

cellulolytic activity,

xylanolytic activity

Related subjects:

Life sciences,

Microbiology and virology

© 2018 ROMAN MARECIK, LIDIA BŁASZCZYK, RÓŻA BIEGAŃSKA-MARECIK, AGNIESZKA PIOTROWSKA-CYPLIK, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 67 (2018): Issue 2 (June 2018)

Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

Figures & Tables

Table I

Fig. 1.

Table II

Paradigm

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