Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

ROMAN MARECIK; LIDIA BŁASZCZYK; RÓŻA BIEGAŃSKA-MARECIK; AGNIESZKA PIOTROWSKA-CYPLIK

doi:10.21307/pjm-2018-021

Figures & Tables

Cellulolytic (part A) and xylanolytic activity (part B) of the studied fungal strains relative to the reference strain T. reesei QM9414 – the plate method analysis. The studied fungal strains belonged to the following species: A – T. viride, B – T. viridescens, C – T. virens, D – T. harzianum, E – T. hamatum, F – T. atroviride, G – T. longibrachiatum, H – T. citrinoviride, I – T. pseudokoningii, J –T. koningii.* The value corresponding to difference in clearing zone diameter between analyzed strains

Total cellulase activities of selected Trichoderma strains measured by the filter paper assay method (FPA)_

Cellulolytic activity (FPU/ml)	Strains of Trichoderma
	T. reesei	T. virens						T. harzianum
	QM 9414	AN 68	AN 69	AN 73	AN 187	AN 188	AN 268	AN 94	AN 108
	2.11 ± 0.25	4.41 ± 0.31	3.21 ± 0.15	6.05 ± 0.46	5.62 ± 0.41	4.42 ± 0.39	6.36 ± 0.48	1.69 ± 0.09	7.15 ± 0.30

List of isolates originating from the different habitats identified as the Trichoderma species and analyzed for their cellulolytic and xylanolytic activity_

Culture code	Origin		NCBI GenBank Assession No.		Cellulolytic activity (the plate method)	Xylanolytic activity (the plate method)
Culture code	Localization	Source	ITS	tef1	Cellulolytic activity (the plate method)	Xylanolytic activity (the plate method)
*T. reesei*
QM9414					1.00	1.00
T. viride (A)
AN109^*, AN176¹AN141AN142AN179AN 235	Central Poland	Forest wood²	HQ292923	HQ293010	0.45/0.50	0.70/0.35
			HQ292922	HQ293008	0.75 ± 0.09	1.75 ± 0.27^a
			HQ292920	HQ293009	2.50 ± 0.31^a	0.67 ± 0.15
			HQ292924	HQ293011	0.56 ± 0.15	0.55 ± 0.18
		Forest soil	HQ292921	HQ293013	0.45 ± 0.15	0.75 ± 0.12
AN242AN244, AN249¹AN250, AN255¹AN371AN401	Southern Poland	Forest wood	JX184121	JX184098	0.55 ± 0.18	0.58 ± 0.15
			JX184122	JX184099	0.46/1.40	0.75/1.42
			JX184121	JX184098	1.03/0.78	0.77/0.81
			JX184124	JX184100	0.91 ± 0.17	0.92 ± 0.09
			JX184121	JX184099	0.75 ± 0.14	0.47 ± 0.21
T. iridescent complex (B)
AN93AN122AN145AN148AN149	Central Poland	Forest wood	HQ292927	HQ292995	0.85 ± 0.21	0.73 ± 0.18
			HQ292928	HQ292994	0.79 ± 0.12	0.57 ± 0.19
			HQ292930	HQ292996	0.85 ± 0.17	0.3 ± 0.33
			HQ292933	HQ292999	0.79 ± 0.23	0.44 ± 0.11
			HQ292934	HQ293000	0.67 ± 0.20	0.48 ± 0.14
AN158^*	Eastern Poland	Wheat kernels	JX184127	JX184103	0.55 ± 0.12	0.76 ± 0.19
AN227AN229AN231	Central Poland	Forest wood	HQ292936	HQ293001	0.67 ± 0.21	0.57 ± 0.13
			HQ292937	HQ293002	0.83 ± 0.16	0.19 ± 0.08
			HQ292938	HQ293003	0.50 ± 0.23	0.58 ± 0.13
AN245AN248AN323, AN334, AN405¹	Southern Poland	Forest wood	JX184127	JX184103	0.50 ± 0.10	0.56 ± 0.16
			JX184128	JX184104	0.63 ± 0.15	0.75 ± 0.23
			JX184127	JX184103	0.78/0.85/0.10	0.58/0.58/0.55
T. vixens (C)
AN68AN69AN70AN73AN74AN75AN160AN185	Eastern Poland	Garden compost	HQ292943	–	2.09 ± 0.27^a	1.10 ± 0.11
			HQ292944	–	1.60 ± 0.19	3.00 ± 0.27^a
			HQ292947	–	2.10 ± 0.22^a	1.10 ± 0.15
			HQ292945	–	2.75 ± 0.31^a	1.10 ± 0.18
			HQ292946	–	1.25 ± 0.12	1.40 ± 0.17
			HQ292948	–	1.53 ± 0.15	1.10 ± 0.13
		Grass root	HQ292945	–	0.83 ± 0.18	0.55 ± 0.18
	Central Poland	Mushroom compost³	HQ292947	–	0.95 ± 0.12	0.55 ± 0.12
AN186^, AN187^,AN188^* AN204^*¹	Eastern Poland		HQ292946	–	0.8/2.6/2.0	1.00/2.25^a/1.15
AN186^, AN187^,AN188^* AN204^*¹	Eastern Poland		HQ292948	–	1.35 ± 0.21	1.45 ± 0.17
AN267 – ITEM 1357AN268 – ITEM 1591AN269 – ITEM 1594	Bari. Italy		–––	–––	1.25 ± 0.172.50 ± 0.23^a1.6 ± 0.19	0.55 ± 0.202.70 ± 0.32^a1.10 ± 0.12
T. harzianum complex (D)
AN91	Central Poland	graden Kompost	HQ292860	–	0.75 ± 0.15	1.15 ± 0.11
AN94		forest soil	HQ292873	–	0.80 ± 0.12	3.55 ± 0.27^a
AN101AN108/AN110^*		forest wood²	HQ292868	–	1.60 ± 0.21	2.58 ± 0.23^a
AN101AN108/AN110^*		forest wood²	HQ292869	–	2.80^a/1.10	1.80^a/1.40
AN131^AN132AN133AN134AN135AN136AN137AN138AN150AN177^AN181	Central Poland	forest wood²	HQ292870	–	1.10 ± 0.16	0.60 ± 0.09
			HQ292867	–	0.78 ± 0.12	0.60 ± 0.07
			HQ292874	–	2.05 ± 0.26^a	1.70 ± 0.21^a
			HQ292875	–	1.01 ± 0.11	1.00 ± 0.17
			HQ292876	–	0.81 ± 0.13	1.65 ± 0.14
			HQ292901	–	2.40 ± 0.22^a	2.04 ± 0.18^a
			HQ292877	–	1.60 ± 0.13	1.03 ± 0.12
			HQ292861	–	1.00 ± 0.10	1.41 ± 0.16
			HQ292878	–	0.87 ± 0.08	1.67 ± 0.16^a
			HQ292883	–	1.25 ± 0.15	1.40 ± 0.11
			HQ292875	–	1.66 ± 0.12^a	2.25 ± 0.22^a
AN203AN205AN207AN211		mushroom compost³	HQ292879	–	1.66 ± 0.13^a	1.00 ± 0.18
			HQ292880	–	1.36 ± 0.17	2.45 ± 0.25^a
			HQ292881	–	1.50 ± 0.13	1.00 ± 0.17
			HQ292882	–	1.10 ± 0.11	0.20 ± 0.11
AN223		Forest soil	HQ292902	–	1.25 ± 0.10	1.05 ± 0.20
AN258AN260		Forest wood	HQ292271	–	0.80 ± 0.08	1.41 ± 0.26
AN258AN260			HQ292884		0.89 ± 0.11	1.20 ± 0.19
AN273AN275AN276AN278AN282AN283AN284AN285AN286			HQ292886	–	1.37 ± 0.19	1.45 ± 0.15
			HQ292888	–	1.20 ± 0.12	1.40 ± 0.11
			HQ292889	–	0.80 ± 0.09	1.05 ± 0.07
			HQ292890	–	0.75 ± 0.10	1.25 ± 0.19
			HQ292891	–	0.30 ± 0.07	1.10 ± 0.12
			HQ292892	–	0.85 ± 0.15	1.27 ± 0.27
			HQ292893	–	1.15 ± 0.20	1.27 ± 0.22
			HQ292894		1.10 ± 0.18	1.28 ± 0.11
			HQ292895	–	1.35 ± 0.29	1.30 ± 0.13
AN349AN360, AN367, AN373, AN3811	Southern Poland	Forest wood	JX184111	JX184089	1.05 ± 0.09	1.38 ± 0.24
AN349AN360, AN367, AN373, AN3811	Southern Poland	Forest wood	JX184113	JX184090	2.20^a/1.70^a/0.70 1.46	1.27/1.40/1.75^a 1.52
AN220 – ITEM 1328	Bari, Italy		–	–	0.25 ± 0.16	1.60 ± 0.13
T. hamatum (E)
AN118	Central Poland	Forest wood²	HQ292854	–	0.66 ± 0.21	0.61 ± 0.19
AN155	Eastern Poland	Ryder Rhizosphäre	HQ292851	–	0.82 ± 0.18	0.51 ± 0.11
AN175AN225, AN238	Central Poland	Forest wood	HQ292854	–	0.53 ± 0.12	0.28 ± 0.10
		Forest soil	HQ292856	–	0.95 ± 0.08	0.75 ± 0.11
		Forest soil	HQ292853		0.41 ± 0.21	0.84 ± 0.29
AN277, AN279, AN501^*¹		Forest wood	HQ292853	–	0.50/0.18/0.41	2.3/0.72/0.61
AN521	Northern Poland	Forest wood	HQ292856	–	1.05 ± 0.24	0.75 ± 0.15
T. atroviride (F)
AN19AN35AN90AN95AN96AN111	Central Poland	Forest soil	HQ292786	HQ292963	0.67 ± 0.13	1.04 ± 0.16
		Maize kernels	HQ292787	HQ292953	0.35 ± 0.09	0.80 ± 0.19
		Garden soil	HQ292788	HQ292954	0.49 ± 0.12	0.35 ± 0.12
		Garden compost	HQ292789	HQ292955	0.90 ± 0.17	1.30 ± 0.18
		Garden compost	HQ292790	HQ292956	0.95 ± 0.15	0.70 ± 0.14
		Forest wood	HQ292791	HQ292964	0.37 ± 0.13	0.75 ± 0.21
AN152 AN153 AN182 AN206 AN212 AN215	Central Poland	Triticale kernel	HQ292792	HQ292957	0.59 ± 0.19	1.85 ± 0.30^a
		Triticale kernel	HQ292793	HQ292958	0.58 ± 0.10	0.57 ± 0.12
		Forest wood	HQ292794	HQ292965	0.57 ± 0.12	0.45 ± 0.23
		Mushroom compost	HQ292804	HQ292960	0.40 ± 0.19	1.02 ± 0.11
			HQ292795	HQ292966	0.68 ± 0.21	1.27 ± 0.21
			HQ292796	HQ292967	1.10 ± 0.16	1.19 ± 0.14
AN224 AN240	Southern Poland	Forest wood	HQ292799	HQ292970	0.36 ± 0.09	0.58 ± 0.13
AN224 AN240	Southern Poland	Forest wood	JX184119	JX184096	0.23 ± 0.19	0.57 ± 0.17
AN287	Central Poland		HQ292798	HQ292969	0.41 ± 0.11	0.58 ± 0.12
T. longibrachiatum (G)
AN197	Eastern Poland	Mushroom factory	HQ292780	–	0.77 ± 0.19	1.40 ± 022
AN213	Central Poland	Mushroom compost³	HQ292781	–	0.50 ± 0.16	2.70 ± 0.31^a
T. citrinoviride (H)
AN89AN98AN198AN199AN201	Central Poland	Garden soil	HQ292841	–	0.50 ± 0.14	1.25 ± 0.27
		Forest wood	HQ292843	–	1.55 ± 0.13	1.07 ± 0.19
		Mushroom factory	HQ292845		0.57 ± 0.17	0.47 ± 0.13
			HQ292846	–	0.95 ± 0.12	2.07 ± 0.26^a
			HQ292849	–	1.10 ± 0.21	2.08 ± 0.29^a
AN262, AN303, AN393, AN500¹	Southern Poland	Forest wood	JX184109	–	2.26^a/0.85/1.75^a 0.90	2.88^a/0.61/0.63 0.80
T. pseudokoningii (I)
AN219 – ITEM1416	Bari. Italy		–	–	1.42 ± 0.19	2.10 ± 0.22^a
T. koningii (J)
AN100 AN105 AN106 AN121 AN128 AN151	Central Poland	Forest wood	HQ292903	HQ292975	0.35 ± 0.17	0.40 ± 0.17
			HQ292905	HQ292977	0.17 ± 0.08	0.58 ± 0.14
			HQ292906	HQ292978	1.16 ± 0.19	0.40 ± 0.16
			HQ292913	HQ292985	0.58 ± 0.11	1.41 ± 0.27
			HQ292918	HQ292989	0.31 ± 0.09	0.48 ± 0.12
			HQ292919	HQ292990	0.47 ± 0.11	0.55 ± 0.12
T. koningiopsis (K)
AN222 – ITEM 2688	Bari, Italy		–	–	0.20 ± 0.10	0.30 ± 0.12

Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

Figures & Tables

Fig. 1.

Total cellulase activities of selected Trichoderma strains measured by the filter paper assay method (FPA)_

List of isolates originating from the different habitats identified as the Trichoderma species and analyzed for their cellulolytic and xylanolytic activity_

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