Table I
The indicator strains used for preliminary selections of staphylococci producing antibacterial agents.
| Strain | Source of the strain | Resistance |
|---|---|---|
| 1 S. xylosus 30c1 | Bovine mastitis | ND |
| 2 S. epidermidis 267 | Bovine mastitis | ND |
| 3 S. aureus K10 | Human skin wound (Koszalin) | Susceptible |
| 4 S. aureus 11 | Bovine mastitis | P, AMP, AML, S, AMC* |
| 5 S. aureus 27 | Bovine mastitis | N, MY, S, E, DA* |
| 6 S. aureus 101 (MRSA) | Bovine mastitis | P+DA+E+MY+S+N/OX* |
| 7 L. monocytogenes ATCC10425 | Reference strain | ND |
1* – Resistance according to Szweda et al. 2014
Fig. 1.
Growth inhibition zone around the colony of producer strain M2B; an indicator strain – S. aureus 30C1.
Fig. 2.
Formation of “half moon zone” near the colony of producer strain M2B in the presence of proteolytic enzyme; an indicator strain – L. monocytogenes.
Fig. 3.
Antimicrobial activity of the after-culture liquid media after growth of the producer strain.
Table II
Activity of newly identified agent against strains from the Departments’ collection.
| No. of strain | Code number of the sensitive strain | Growth inhibition zone [mm] |
|---|---|---|
| 1 | 7 (S. aureus) | 12 |
| 2 | 11 (S. aureus) | 20 |
| 3 | 28 (S. aureus) | 15 |
| 4 | 52 (S. aureus) | 22 |
| 5 | 68 (S. aureus) | 25 |
| 6 | 77 (S. aureus) | 18 |
| 7 | 92 (S. aureus) | 17 |
| 8 | 99 (S. aureus) | 25 |
| 9 | 115 (S. aureus) | 15 |
| 10 | 83A (S. epidermidis) | 15 |
| 11 | 8J (S. xylosus) | 15 |
| 12 | 30C1 (S. xylosus) | 15 |
| 13 | 247 (S. xylosus) | 15 |
| 14 | L. monocytogenes | 15 |
| 15 | M. luteus | 15 |