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Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis Cover

Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

Open Access
|Jun 2018

Figures & Tables

Fig. 1.

Growth inhibition zone around the colony of producer strain M2B; an indicator strain – S. aureus 30C1.
Growth inhibition zone around the colony of producer strain M2B; an indicator strain – S. aureus 30C1.

Fig. 2.

Formation of “half moon zone” near the colony of producer strain M2B in the presence of proteolytic enzyme; an indicator strain – L. monocytogenes.
Formation of “half moon zone” near the colony of producer strain M2B in the presence of proteolytic enzyme; an indicator strain – L. monocytogenes.

Fig. 3.

Antimicrobial activity of the after-culture liquid media after growth of the producer strain.
Antimicrobial activity of the after-culture liquid media after growth of the producer strain.

The indicator strains used for preliminary selections of staphylococci producing antibacterial agents_

StrainSource of the strainResistance
1 S. xylosus 30c1Bovine mastitisND
2 S. epidermidis 267Bovine mastitisND
3 S. aureus K10Human skin wound (Koszalin)Susceptible
4 S. aureus 11Bovine mastitisP, AMP, AML, S, AMC*
5 S. aureus 27Bovine mastitisN, MY, S, E, DA*
6 S. aureus 101 (MRSA)Bovine mastitisP+DA+E+MY+S+N/OX*
7 L. monocytogenes ATCC10425Reference strainND

Activity of newly identified agent against strains from the Departments’ collection_

No. of strainCode number of the sensitive strainGrowth inhibition zone [mm]
  1    7 (S. aureus)12
  2  11 (S. aureus)20
  3  28 (S. aureus)15
  4  52 (S. aureus)22
  5  68 (S. aureus)25
  6  77 (S. aureus)18
  7  92 (S. aureus)17
  8  99 (S. aureus)25
  9115 (S. aureus)15
1083A (S. epidermidis)15
11   8J (S. xylosus)15
1230C1 (S. xylosus)15
13247 (S. xylosus)15
14L. monocytogenes15
15M. luteus15
DOI: https://doi.org/10.21307/pjm-2018-018 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 163 - 170
Submitted on: Aug 8, 2017
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Accepted on: Nov 20, 2017
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Published on: Jun 30, 2018
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2018 MAGDALENA ZALEWSKA, JOHN J. CHUREY, RANDY W. WOROBO, SŁAWOMIR MILEWSKI, PIOTR SZWEDA, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.