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The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia) Cover

The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

Open Access
|Dec 2021

Figures & Tables

Figure 1:

Sampling site (A) of C. farionis (C) parasitizing the swim bladder of different morphs of the nosed charr (B). Picture by P.G. Vlasenko.
Sampling site (A) of C. farionis (C) parasitizing the swim bladder of different morphs of the nosed charr (B). Picture by P.G. Vlasenko.

Figure 2:

18S rRNA gene tree reconstruction based on ML methods.
18S rRNA gene tree reconstruction based on ML methods.

Figure 3:

Diversity analysis of microbial community associated with nematodes Cystidicola farionis. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—“body”, green—swim bladder, blue—gut. The asterisk character indicates significance at p ≤ 0.05.
Diversity analysis of microbial community associated with nematodes Cystidicola farionis. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—“body”, green—swim bladder, blue—gut. The asterisk character indicates significance at p ≤ 0.05.

Figure 4:

Diversity analysis of the microbial community associated with nematodes Cystidicola farionis parasitizing the swim bladder of two morphs of nosed charr. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—morph N1g of nosed charr, blue—morph N2 of nosed charr. The asterisk character indicates significance at p ≤ 0.05.
Diversity analysis of the microbial community associated with nematodes Cystidicola farionis parasitizing the swim bladder of two morphs of nosed charr. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—morph N1g of nosed charr, blue—morph N2 of nosed charr. The asterisk character indicates significance at p ≤ 0.05.

Figure 5:

Taxonomical composition of microbial communities identified in gut, “body” remains of Cystidicola farionis and microbiota of swim bladder of nosed charr. Numbers 1 to 4 along x-axis denotes the number of individual of nosed charr per pool; N1g/N2 indicates an ecological group of fish, «whole» indicates whole nematode without dissection of their “body”. (A) Phylum composition: () Actinobacteria, () Bacteroidetes, () Chloroflexi, () Firmicutes, () Fusobacteria, () Proteobacteria, (), Others<1%. (b) Genus and family proportions: () Corynebacterium 1, () Lawsonella, () Cutibacterium, () Nodosilinea PCC-7104, () Tumebacillus, () unc. Alicyclobacillaceae, () Bacillus, () Staphylococcus, () Cetobacterium, () Caulobacter, () Paracoccus, () Pajaroellobacter, () Aeromonas, () Shewanella, () Ambiguous taxa of Enterobacteriaceae, () Yersinia, () unc. other Enterobacteriaceae, () Acinetobacter, () Pseudomonas, () unc. Gammaproteobacteria, () Other < 3%.
Taxonomical composition of microbial communities identified in gut, “body” remains of Cystidicola farionis and microbiota of swim bladder of nosed charr. Numbers 1 to 4 along x-axis denotes the number of individual of nosed charr per pool; N1g/N2 indicates an ecological group of fish, «whole» indicates whole nematode without dissection of their “body”. (A) Phylum composition: () Actinobacteria, () Bacteroidetes, () Chloroflexi, () Firmicutes, () Fusobacteria, () Proteobacteria, (), Others<1%. (b) Genus and family proportions: () Corynebacterium 1, () Lawsonella, () Cutibacterium, () Nodosilinea PCC-7104, () Tumebacillus, () unc. Alicyclobacillaceae, () Bacillus, () Staphylococcus, () Cetobacterium, () Caulobacter, () Paracoccus, () Pajaroellobacter, () Aeromonas, () Shewanella, () Ambiguous taxa of Enterobacteriaceae, () Yersinia, () unc. other Enterobacteriaceae, () Acinetobacter, () Pseudomonas, () unc. Gammaproteobacteria, () Other < 3%.

Nearest-match identification of C_ farionis to known sequences in NCBI database_

GenBank acc. no.GeneSpecies
JF803919.1small subunit ribosomal RNA gene (18S) partial C. farionis
MG594291.1small subunit ribosomal RNA gene (18S) partial C. farionis
DQ094172.1small subunit ribosomal RNA gene (18S) partial Ascarophis arctica
MN294781.1small subunit ribosomal RNA gene (18S) partial Comephoronema werestschagini
MN294783.1small subunit ribosomal RNA gene (18S) partial Capillospirura ovotrichuria
MG594289.1small subunit ribosomal RNA gene (18S) partial Capillospirura sp.
JF934733.1small subunit ribosomal RNA gene (18S) partial Proleptus sp.
DQ442660.1small subunit ribosomal RNA gene (18S) partial Neoascarophis macrouri
JF803921.1small subunit ribosomal RNA gene (18S) partial N. longispicula
JF803926.1small subunit ribosomal RNA gene (18S) partial Heliconema longissimum
JF803949.1small subunit ribosomal RNA gene (18S) partial H. longissimum
JF803930.1small subunit ribosomal RNA gene (18S) partial Ascarophis adioryx
MT086834.1large subunit ribosomal RNA gene (28S) partial C. farionis

Descriptions of samples used in this study_

Ecological group of fishSexBody weight, gTotal length, mmFork length, mmType of sample for microbiome analysisNumber of pooled samples
1-N1gF304.4355.0309.0Gut15
Gut15
“Body”15
“Body”15
Swim bladder1
2-N1gM652.1438.0400.0Gut20
“Body”20
Swim bladder1
3-N2M229.6292.0260.0Gut5
Gut5
Gut10
Gut25
“Body”5
“Body”5
“Body”10
“Body”25
Swim bladder1
4-N2F294.3295.0333.0Gut20
“Body”20
Swim bladder1

Prevalence, intensity and mean abundance of C_ farionis in swim bladder of three morphs of nosed charr at different sampling times_

YearMonthPrevalence, %IntensityMean abundanceNumber of fish
Nosed charr N1 (n = 227) a
 2013VI-VIII690–32883.0 ± 3.913
 2014VI960–452144.0 ± 15.449
VII840–335131.0 ± 13.650
VIII870–526150.0 ± 18.238
IXb
X940–550140.0 ± 18.247
XI10012–508154.9 ± 23.430
Nosed charr N1g (n = 2)
 2020IX-X10015–2016.6 ± 5.02
Nosed charr N2 (n = 57)
 2013VI-VIII820–685172.0 ± 44.817
 2014VI630–274126.0 ± 42.08
VII800–362179.0 ± 38.910
VIII10043–252118.0 ± 28.88
IX1002323.01
X100130–376243.0 ± 33.18
XI100155–466333.0 ± 92.03
 2020IX-X1005–4532.5 ± 12.52
Nosed charr N3 (n = 37)
 2013VI-VIII880–519205.0 ± 58.28
 2014VI
VII10028–225130.0 ± 44.54
VIII100420420.01
IX740–466102.0 ± 24.0423
X100318318.01
XI
 2020IX-X
DOI: https://doi.org/10.21307/jofnem-2021-106 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 15
Published on: Dec 15, 2021
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2021 E.N. Kashinskaya, E.P. Simonov, P.G. Vlasenko, G.N. Markevich, A.V. Shokurova, K.B. Andree, M.M. Solovyev, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.