Table 1.
Pratylenchus specimens used for phylogenetic analysis in this study.
| NID # | Species | Stage | Locality | Host | Marker | GenBank# |
|---|---|---|---|---|---|---|
| N3873 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | MK878313 |
| N3873 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | rDNA LSU | OK490313 |
| N3880 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | MK878314 |
| N3880 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | ITS1 | OK490336 |
| N8136 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | MK878315 |
| N8137 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878316 |
| N8139 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878317 |
| N8139 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490314 |
| N8145 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878318 |
| N8145 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490315 |
| N8146 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878319 |
| N8148 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878320 |
| N8154 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878321 |
| N8156 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878322 |
| N8170 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | MK878323 |
| N8170 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490316 |
| N9793 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489823 |
| N9793 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490317 |
| N9793 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA SSU | OK490342 |
| N9795 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | rDNA SSU | OK490343 |
| N9809 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | ITS1 | OK490337 |
| N10037 | P. smoliki n. sp. | F | Buffalo Co., NE | Corn | COI | MK878324 |
| N10039 | P. smoliki n. sp. | F | Buffalo Co., NE | Corn | COI | MK878325 |
| N10053 | P. smoliki n. sp. | F | Buffalo Co., NE | Corn | COI | MK878326 |
| N11506 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489824 |
| N11506 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490318 |
| N11507 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489825 |
| N11508 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489826 |
| N11509 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489827 |
| N11509 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490319 |
| N11510 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489828 |
| N11592 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489829 |
| N11593 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489830 |
| N11593 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490320 |
| N11594 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA SSU | OK490344 |
| N11595 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489831 |
| N11595 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490321 |
| N11600 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489832 |
| N11602 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489833 |
| N11603 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490322 |
| N11605 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489834 |
| N11607 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489835 |
| N11612 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489836 |
| N11613 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489837 |
| N11614 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489838 |
| N11614 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490323 |
| N11618 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489839 |
| N12448 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489840 |
| N12451 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489841 |
| N12451 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489842 |
| N12453 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489843 |
| N12453 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | rDNA LSU | OK490324 |
| N12454 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | ITS1 | OK490338 |
| N12457 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489844 |
| N12458 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489845 |
| N12459 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489846 |
| N12463 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489847 |
| N12463 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | rDNA LSU | OK490325 |
| N12464 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489848 |
| N12465 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489849 |
| N12466 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489850 |
| N12467 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489851 |
| N12468 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489852 |
| N12469 | P. smoliki n. sp. | M | Shawnee Co., KS* | Corn | COI | OK489853 |
| N12470 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489854 |
| N12470 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | rDNA LSU | OK490326 |
| N12471 | P. smoliki n. sp. | F | Shawnee Co., KS* | Corn | COI | OK489855 |
| N3717 | P. alleni | M | Madison Co., NE | Corn | COI | MK877458 |
| N3717 | P. alleni | M | Madison Co., NE | Corn | rDNA LSU | OK490293 |
| N7381 | P. alleni | F | Saline Co., IL** | Soybean | COI | MK877459 |
| N7382 | P. alleni | M | Saline Co., IL** | Soybean | COI | MK877460 |
| N10848 | P. alleni | J | Saline Co., IL** | Soybean | rDNA LSU | OK490294 |
| N10850 | P. alleni | F | Saline Co., IL** | Soybean | COI | MK877461 |
| N10850 | P. alleni | F | Saline Co., IL** | Soybean | rDNA LSU | OK490295 |
| N8539 | P. crenatus | F | County Galway, IRE | Pasture | rDNA LSU | OK490296 |
| N9774 | P. crenatus | J | Rwanda | - | COI | OK489810 |
| N6350 | P. dakotaensis | F | Atchison Co., KS | Corn | rDNA LSU | OK490297 |
| N6351 | P. dakotaensis | J | Atchison Co., KS | Corn | rDNA LSU | OK490298 |
| N6438 | P. hexincisus | F | Graham Co., KS | Corn | rDNA LSU | OK490299 |
| N7726 | P. hexincisus | F | Big Horn Co., WY | Drybean | rDNA LSU | OK490300 |
| N10849 | P. hexincisus | J | Saline Co., IL | Soybean | rDNA LSU | OK490301 |
| N10756 | P. neglectus | F | Seward Co., KS | Corn | rDNA LSU | OK490302 |
| N11481 | P. neglectus | F | Gallatin Co., MT | Corn | COI | OK489811 |
| N11483 | P. neglectus | F | Gallatin Co., MT | Corn | COI | OK489812 |
| N11485 | P. neglectus | F | Broadwater Co., MT | Corn | COI | OK489813 |
| N11487 | P. neglectus | F | Broadwater Co., MT | Corn | COI | OK489814 |
| N11489 | P. neglectus | F | Broadwater Co., MT | Corn | COI | OK489815 |
| N11491 | P. neglectus | F | Treasure Co., MT | Corn | COI | OK489816 |
| N11499 | P. neglectus | F | Yellowstone Co., MT | Corn | COI | OK489817 |
| N6260 | P. penetrans | M | Fairbanks Co., AK | Peony | rDNA LSU | OK490303 |
| N6261 | P. penetrans | J | Fairbanks Co., AK | Peony | rDNA LSU | OK490304 |
| N7091 | P. penetrans | F | Fairbanks Co., AK | Peony | rDNA LSU | OK490305 |
| N11414 | P. penetrans | F | British Columbia, CAN | Prunus sp. | COI | OK489818 |
| N11415 | P. penetrans | F | British Columbia, CAN | Prunus sp. | COI | OK489819 |
| N11416 | P. penetrans | F | British Columbia, CAN | Prunus sp. | COI | OK489820 |
| N11417 | P. penetrans | F | British Columbia, CAN | Prunus sp. | COI | OK489821 |
| N11424 | P. penetrans | F | British Columbia, CAN | Prunus sp. | COI | OK489822 |
| N6383 | P. scribneri | F | Marshall Co., KS | Corn | rDNA LSU | OK490306 |
| N7833 | P. scribneri | J | Custer Co., NE | Corn | rDNA LSU | OK490307 |
| N7839 | P. scribneri | J | Custer Co., NE | Corn | rDNA LSU | OK490308 |
| N7845 | P. scribneri | F | Custer Co., NE | Corn | rDNA LSU | OK490309 |
| N10274 | P. scribneri | F | Kearney Co., NE | Corn | rDNA LSU | OK490310 |
| N10301 | P. scribneri | F | Phelps Co., NE | Corn | rDNA LSU | OK490311 |
| N10306 | P. scribneri | F | Phelps Co., NE | Corn | rDNA LSU | OK490312 |
| P130021 | P. scribneri | - | Maryland | - | ITS1 | OK490333 |
| P156024 | P. scribneri | F | Florida | - | ITS1 | OK490334 |
| P344041 | P. scribneri | - | - | - | ITS1 | OK490335 |
| N7566 | P. thornei | F | Treasure Co., MT | Corn | rDNA LSU | OK490327 |
| N7567 | P. thornei | F | Treasure Co., MT | Corn | rDNA LSU | OK490328 |
| N11495 | P. thornei | F | Yellowstone Co., MT | Corn | COI | OK489856 |
| N11497 | P. thornei | F | Yellowstone Co., MT | Corn | COI | OK489857 |
| N11422 | P. vulnus | F | British Columbia, CAN | Prunus sp. | COI | OK489858 |
| N10764 | Pratylenchus sp. | F | Sumner Co., KS | Corn | rDNA LSU | OK490329 |
| N12456 | Pratylenchus sp. | M | Shawnee Co., KS | Corn | COI | OK489859 |
| N10685 | Pratylenchus sp. | F | Seward Co., KS | Corn | rDNA LSU | OK490330 |
| N8930 | Pratylenchus sp. | F | Lincoln Co., AR | Soybean | rDNA LSU | OK490331 |
| N10841 | Pratylenchus sp. | J | Cross Co., AR | Soybean | rDNA LSU | OK490332 |
| P156016 | Pratylenchus sp. | F | Lancaster Co., NE | Leadplant | ITS1 | OK490339 |
| P156019 | Pratylenchus sp. | F | Gage Co., NE | Big Bluestem | ITS1 | OK490340 |
| P201006 | Pratylenchus sp. | - | Idaho | - | ITS1 | OK490341 |
| N84 | Nacobbus aberrans | M | Xalatlaco, MEX | - | COI | OK489860 |
| N85 | Nacobbus aberrans | M | Xalatlaco, MEX | - | COI | OK489861 |
| N194 | Nacobbus aberrans | J | Sioux Co., NE | Sugarbeet | COI | OK489862 |
| N452 | Nacobbus aberrans | - | Zacatecas, MEX | Drybean | COI | OK489863 |
| N537 | Nacobbus aberrans | J | Chacra, ARG | Tomato | COI | OK489864 |
| N658 | Nacobbus aberrans | J | Lisandro Olmos, ARG | - | COI | OK489865 |
1 Note: *Type locality for Pratylenchus smoliki n. sp. Kansas River Valley Experiment Field, within the 80 acre Paramore Unit, located 3.5 miles east of Silver Lake on U.S. Highway 24, then 1 mile south of Kiro, and 1.5 miles east on 17th Street. **Type locality for Pratylenchus alleni. Soybean field 5 miles north of Eldorado, Illinois. Ferris, V. R. (1961).
Table 2.
Primers.
| Marker | Primer set | Amplicon Size (kb) | Primer Sequence (5′→ 3′) | Direction | Reference |
|---|---|---|---|---|---|
| COIa | PsmoF4 | 0.42 | 5′-ATY GCS CCC GCC TTT GG-3’ | Forward | This manuscript |
| COI | JB5 | 5′-AGC ACC TAA ACT TAA AAC ATA ATG AAA ATG-3′ | Reverse | Derycke et al. (2005) | |
| COI | JB3 | 0.43 | 5′-TTTTTTGGGCATCCTGAGGTTTAT-3′ | Forward | Bowles et al., (1992) |
| COI | JB5 | 5′-AGCACCTAAACTTAAAACATAATGAAAATG-3′ | Reverse | Derycke et al. (2005) | |
| COI | F1KF | 0.95 | 5′- CCTACTATGATTGGTGGTTTTGGTAATTG-3′ | Forward | Kanzaki & Futai (2002) |
| COI | JB5 | 5′-AGCACCTAAACTTAAAACATAATGAAAATG-3′ | Reverse | Derycke et al. (2005) | |
| COI | F7bPrat | 0.78 | 5′-GGDTGRACWTTHTAYCCNCC-3′ | Forward | Ozbayrak et al. (2019) |
| COI | JB5 | 5′-AGCACCTAAACTTAAAACATAATGAAAATG-3′ | Reverse | Derycke et al. (2005) | |
| ITS1b | rDNA2 | 0.62 | 5′-TTGATTACGTCCCTGCCCTTT-3′ | Forward | Vrain et al. (1992) |
| ITS1 | rDNA1.58Sa | 5′-ACGAGCCGAGTGATCCACC-3′ | Reverse | Cherry et al. (1997) | |
| rDNA LSUc | D2A | 0.75 | 5′-ACAAGTACCGTGAGGGAAAGTTG-3′ | Forward | De Ley et al. (1999) |
| rDNA LSU | D3B | 5′-TCGGAAGGAACCAGCTACTA-3 | Reverse | De Ley et al. (1999) | |
| rDNA LSU | 28s-PratF3 | 0.48 | 5′-TTTGCAAGTGGAGTGCGT-3′ | Forward | This manuscript |
| rDNA LSU | 28s-PratR1 | 5′-AATAGTTCACCATCTTTCGGG -3′ | Reverse | This manuscript |
Figure 1:
Light microscope images of Pratylenchus smoliki n. sp. specimens. (A) NID 8139 female head, (B) NID 8139 female tail, (C) NID 8139 spermatheca, vulva and post-uterine sac, (D) NID 8170 female reproductive system, (E) NID 8170 female entire body, (F) NID 8137 male entire body, (G) NID 8154 spicules and bursa, (H) NID 8154 male head, (I) NID 8137 spicules and bursa, (J) NID 8154 midbody lateral lines in male.
Figure 2:
P. smoliki n. sp. SEM in en face view, different NID numbers specify different individual specimens. (A) NID 4808 Trapezoidal subdorsal and subventral lips adjoining oral disc with demarcation between sectors, six inner labial sensilla flank oral opening, (B) NID 4801 Lateral lip sectors clearly separated from oral disc and subventral/subdorsal sectors, (C) NID 4805 and (D) NID 4820 Anterior region profiles displaying two lip annuli.
Figure 3:
P. smoliki n. sp. SEM of anterior region with beginning of lateral field, different NID numbers specify different individual specimens. (A) NID 4815, (B) NID 4812, (C) NID 4816, (D) NID 4817.
Figure 4:
P. smoliki n. sp. SEM of midbody region, different NID numbers specify different individual specimens. (A) NID 4808 and (B) NID 4818 slightly protruding vulval lips, (C) NID 4807 and (D) NID 4803 lateral field with irregularly areolated lines at midbody.
Figure 5:
SEM images of P. smoliki n. sp. tails, different NID numbers specify different individual specimens. A–I exhibit the variation in tail termini, J–L illustrate male tails. (A) NID 4803, (B) NID 4819, (C) NID 4813, (D) NID 4818, (E) NID 4805, (F) NID 4811, (G) NID 4809, (H) NID 4803, (I) NID 4805, (J) NID 4817, (K) NID 4816, (L) NID 4816.
Table 3.
Measurements of P. smoliki n. sp. specimens.
| Holotype | N | Females | N | Males | N | Juveniles | |
|---|---|---|---|---|---|---|---|
| L | 491 | 22 | 511.4 + 50.6 (407-604) | 25 | 456.8 + 32.7 (415-560) | 4 | 320.5 + 38.1 (289-385) |
| a | 21.0 | 22 | 24.4 + 3.0 (18.8-31.9) | 25 | 27.1 + 1.7 (23.8-31.7) | 4 | 20.5 + 2.3 (18.0-24.1) |
| b | 5.4 | 21 | 5.6 + 0.4 (4.8-6.6) | 25 | 5.3 + 0.4 (4.4-5.9) | 4 | 3.7 + 0.5 (3.3-4.5) |
| b′ | 4.2 | 22 | 4.3 + 0.4 (3.5-4.9) | 25 | 4.1 + 0.3 (3.6-4.6) | 4 | 3.0 + 0.3 (2.5-3.4) |
| c | 19.5 | 22 | 22.1 + 2.8 (17.7-31.5) | 25 | 22.6 + 3.9 (16.7-34.1) | 4 | 17.7 + 2.2 (15.7-21.5) |
| c′ | 1.9 | 22 | 1.9 + 0.2 (1.6-2.2) | 24 | 1.6 + 0.2 (1.2-2.2) | 4 | 1.8 + 0.1 (1.7-1.9) |
| V % | 79.4 | 22 | 79.2 + 1.4 (76.8-82.0) | ||||
| Lip annules | 2 | 22 | 2.0 + 0 (2-2) | 25 | 2.0 + 0 (2-2) | 4 | 2.0 + 0 (2-2) |
| St | 16 | 22 | 15.1 + 0.6 (14-16) | 25 | 14.2 + 0.6 (13-15) | 4 | 12.7 + 0.6 (12-14) |
| M % | 50 | 22 | 48.3 + 1.6 (44.9-51.7) | 25 | 47.7 + 2.9 (43.1-54.7) | 4 | 44.9 + 2.5 (41.9-48.7) |
| DGO | 3 | 22 | 2.7 + 0.3 (2-3) | 25 | 2.9 + 0.1 (3-3) | 4 | 2.3 + 0.1 (2-3) |
| O % | 17.3 | 22 | 17.7 + 1.9 (12.7-20.3) | 25 | 20.0 + 1.2 (18.3-23.0) | 4 | 18.3 + 1.3 (16.5-20.0) |
| MB % | 45.1 | 22 | 44.1 + 2.5 (39.4-51.7) | 25 | 44.6 + 2.6 (39.8-50.1) | 4 | 42.0 + 3.2 (39.2-47.1) |
| Overlap | 22 | 29.1 + 6.5 (16-41) | 25 | 26.2 + 6.4 (14-39) | 4 | 19.4 + 8.3 (7-27) | |
| Body ann W | 1.6 | 22 | 1.4 + 0.2 (1.0-2.0) | 24 | 1.3 + 0.1 (1.0-1.5) | 4 | 1.2 + 0.1 (1.0-1.3) |
| LFL | 4 | 24 | 4.0 + 0.0 (4-4) | ||||
| PUS/Bw | 0.6 | 22 | 0.9 + 0.2 (1-1) | ||||
| PUS/V-A % | 16.1 | 22 | 22.5 + 6.4 (13.5-32.8) | ||||
| Sthc L/W | 22 | 1.1 + 0.1 (0.9-1.4) | |||||
| T % | 25 | 44.1 + 4.8 (35.0-55.0) | |||||
| Spicule | 25 | 16.8 + 0.8 (15-19) | |||||
| Gubernaculum | 24 | 4.3 + 0.6 (3-6) | |||||
| Bursa: % tail | 25 | 96.5 + 9.2 (80.5-136.5) | |||||
| Tail | 25 | 22 | 23.4 + 2.8 (17-29) | 25 | 20.7 + 3.2 (13-28) | 4 | 18.2 + 0.4 (18-19) |
| T/VA | 0.3 | 22 | 0.3 + 0.0 (0.2-0.4) | ||||
| Ran | 21 | 22 | 19.7 + 3.0 (14-26) | 6 | 18.6 + 3.0 (15-24) | 4 | 18.0 + 1.2 (16-19) |
Figure 6:
Light microscope images of Pratylenchus alleni specimens from the type locality Saline County, Illinois. (A–E) female NID 7381, (F–H) male NID 7382. (A) Entire body, (B) Head and anterior region, (C) Reproductive region, (D) Midbody lateral lines, (E) Tail, (F) Tail, bursa and spicules, (G) Head and anterior portion, (H) Entire body
Figure 7:
A-C, COI DNA Maximum likelihood tree of 372 Pratylenchid sequences GTR + G 200, the tree is split into three sections, 7A, 7B, and 7C. Bootstrap values in red, brackets delimit species and haplotype groups.
Figure 8:
28S DNA Maximum likelihood tree of 41 Pratylenchid sequences K2P + G 500 Bootstrap values in red, brackets delimit species and haplotype groups.
Table 4.
Estimates of evolutionary divergence (p-distances) over sequence pairs within (bold) and between groups.
| N. aberrans | P. alleni | P. crenatus | P. hexicisus | P. neglectus | P. penetrans | P. scribneri | P. sp. 3 | P. sp Clay | P. dakotaensis | P. smoliki n. sp. | P. sp. 9 & 10 | P. sp. 7 | P. sp. 5 | P. thornei | P. vulnus | P. zeae | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nacobbus aberrans | 0.105 | ||||||||||||||||
| P. alleni | 0.328 | 0.003 | |||||||||||||||
| P. crenatus | 0.358 | 0.316 | 0.001 | ||||||||||||||
| P. hexincisus | 0.416 | 0.217 | 0.36 | 0.029 | |||||||||||||
| P. neglectus | 0.314 | 0.3 | 0.36 | 0.381 | 0.02 | ||||||||||||
| P. penetrans | 0.325 | 0.323 | 0.32 | 0.379 | 0.31 | 0.032 | |||||||||||
| P. scribneri | 0.36 | 0.172 | 0.32 | 0.198 | 0.329 | 0.334 | 0.007 | ||||||||||
| P. sp. 3 | 0.365 | 0.181 | 0.356 | 0.23 | 0.339 | 0.348 | 0.187 | 0.004 | |||||||||
| P._sp_Clay | 0.373 | 0.188 | 0.329 | 0.196 | 0.329 | 0.339 | 0.112 | 0.191 | 0 | ||||||||
| P. dakotaensis | 0.335 | 0.216 | 0.306 | 0.286 | 0.322 | 0.307 | 0.242 | 0.228 | 0.247 | 0.078 | |||||||
| P. smoliki n. sp. | 0.412 | 0.219 | 0.381 | 0.21 | 0.377 | 0.362 | 0.227 | 0.19 | 0.221 | 0.298 | 0.101 | ||||||
| P. sp. 9 & 10 | 0.378 | 0.204 | 0.336 | 0.23 | 0.355 | 0.35 | 0.21 | 0.226 | 0.205 | 0.254 | 0.229 | 0.105 | |||||
| P. sp. 7 | 0.351 | 0.171 | 0.303 | 0.215 | 0.311 | 0.321 | 0.142 | 0.188 | 0.15 | 0.212 | 0.235 | 0.212 | 0.091 | ||||
| P. sp. 5 | 0.38 | 0.195 | 0.333 | 0.179 | 0.358 | 0.349 | 0.127 | 0.2 | 0.079 | 0.26 | 0.206 | 0.204 | 0.161 | 0 | |||
| P. thornei | 0.311 | 0.34 | 0.372 | 0.386 | 0.298 | 0.311 | 0.355 | 0.352 | 0.348 | 0.32 | 0.373 | 0.349 | 0.328 | 0.366 | 0.002 | ||
| P. vulnus | 0.335 | 0.241 | 0.321 | 0.3 | 0.333 | 0.305 | 0.265 | 0.287 | 0.276 | 0.241 | 0.319 | 0.259 | 0.246 | 0.297 | 0.316 | 0.051 | |
| P. zeae | 0.326 | 0.326 | 0.346 | 0.398 | 0.315 | 0.331 | 0.352 | 0.332 | 0.34 | 0.314 | 0.394 | 0.36 | 0.331 | 0.36 | 0.317 | 0.327 | 0.012 |
| P. horti | 0.331 | 0.26 | 0.331 | 0.296 | 0.317 | 0.31 | 0.243 | 0.23 | 0.244 | 0.217 | 0.308 | 0.246 | 0.228 | 0.277 | 0.295 | 0.254 | 0.299 |
| P. fallax | 0.279 | 0.302 | 0.292 | 0.359 | 0.278 | 0.153 | 0.311 | 0.332 | 0.321 | 0.271 | 0.39 | 0.342 | 0.297 | 0.346 | 0.272 | 0.296 | 0.309 |
| P. pseudocoffeae | 0.326 | 0.18 | 0.339 | 0.22 | 0.326 | 0.326 | 0.193 | 0.181 | 0.173 | 0.243 | 0.229 | 0.222 | 0.184 | 0.197 | 0.345 | 0.277 | 0.331 |
| P. coffeae | 0.314 | 0.231 | 0.28 | 0.302 | 0.324 | 0.293 | 0.229 | 0.249 | 0.237 | 0.224 | 0.291 | 0.24 | 0.224 | 0.257 | 0.304 | 0.244 | 0.303 |
| P. hippeastri | 0.307 | 0.234 | 0.318 | 0.3 | 0.325 | 0.307 | 0.247 | 0.246 | 0.255 | 0.201 | 0.278 | 0.256 | 0.228 | 0.267 | 0.326 | 0.246 | 0.317 |
| P. loosi | 0.301 | 0.223 | 0.311 | 0.302 | 0.281 | 0.302 | 0.242 | 0.236 | 0.246 | 0.215 | 0.301 | 0.248 | 0.235 | 0.263 | 0.298 | 0.271 | 0.296 |
| P. convallariae | 0.315 | 0.337 | 0.316 | 0.385 | 0.316 | 0.167 | 0.329 | 0.349 | 0.333 | 0.291 | 0.389 | 0.346 | 0.308 | 0.351 | 0.31 | 0.305 | 0.323 |
| P. sp | 0.324 | 0.222 | 0.287 | 0.284 | 0.309 | 0.309 | 0.228 | 0.242 | 0.254 | 0.204 | 0.288 | 0.242 | 0.227 | 0.252 | 0.32 | 0.25 | 0.296 |
| P. speijeri | 0.306 | 0.235 | 0.29 | 0.274 | 0.307 | 0.312 | 0.216 | 0.253 | 0.233 | 0.198 | 0.3 | 0.236 | 0.224 | 0.248 | 0.305 | 0.231 | 0.306 |
| P. capsici | 0.299 | 0.313 | 0.329 | 0.365 | 0.296 | 0.194 | 0.312 | 0.319 | 0.317 | 0.277 | 0.368 | 0.342 | 0.306 | 0.339 | 0.296 | 0.3 | 0.326 |
| P. brachyurus | 0.316 | 0.323 | 0.339 | 0.396 | 0.31 | 0.233 | 0.341 | 0.341 | 0.366 | 0.296 | 0.388 | 0.371 | 0.328 | 0.369 | 0.283 | 0.317 | 0.328 |
| P. flakkensis | 0.317 | 0.342 | 0.377 | 0.392 | 0.352 | 0.305 | 0.356 | 0.349 | 0.343 | 0.324 | 0.384 | 0.354 | 0.336 | 0.371 | 0.292 | 0.335 | 0.332 |
| P. parazeae | 0.386 | 0.368 | 0.394 | 0.398 | 0.366 | 0.385 | 0.368 | 0.355 | 0.368 | 0.353 | 0.398 | 0.388 | 0.359 | 0.374 | 0.346 | 0.359 | 0.264 |
| P. horti | P. fallax | P. pseudocoffeae | P. coffeae | P. hippeastri | P. loosi | P. convallariae | P. sp. C1 | P. speijeri | P. capsici | P. brachyurus | P. flakkensis | P. parazeae | |||||
| P. horti | 0.001 | ||||||||||||||||
| P. fallax | 0.292 | 0.007 | |||||||||||||||
| P. pseudocoffeae | 0.248 | 0.325 | 0 | ||||||||||||||
| P. coffeae | 0.229 | 0.291 | 0.252 | 0.008 | |||||||||||||
| P. hippeastri | 0.224 | 0.304 | 0.256 | 0.185 | 0.023 | ||||||||||||
| P. loosi | 0.221 | 0.305 | 0.249 | 0.193 | 0.209 | 0.026 | |||||||||||
| P. convallariae | 0.305 | 0.145 | 0.331 | 0.279 | 0.297 | 0.306 | 0.021 | ||||||||||
| P. sp. C1 | 0.238 | 0.298 | 0.27 | 0.143 | 0.197 | 0.193 | 0.296 | 0.018 | |||||||||
| P. speijeri | 0.215 | 0.294 | 0.241 | 0.118 | 0.164 | 0.181 | 0.283 | 0.109 | 0.005 | ||||||||
| P. capsici | 0.276 | 0.169 | 0.334 | 0.295 | 0.278 | 0.284 | 0.187 | 0.292 | 0.28 | 0.006 | |||||||
| P. brachyurus | 0.308 | 0.202 | 0.356 | 0.308 | 0.316 | 0.296 | 0.229 | 0.298 | 0.279 | 0.211 | 0.014 | ||||||
| P. flakkensis | 0.314 | 0.292 | 0.356 | 0.299 | 0.291 | 0.292 | 0.337 | 0.335 | 0.333 | 0.289 | 0.315 | 0.04 | |||||
| P. parazeae | 0.352 | 0.377 | 0.389 | 0.361 | 0.373 | 0.37 | 0.389 | 0.353 | 0.357 | 0.357 | 0.353 | 0.356 | 0.049 |
Figure S1:
Maximum likelihood tree of ITS1 sequences from P. smoliki n. sp. and closely related species. Bootstrap values in red.
Figure 9:
Box and whisker plots of the distribution of numbers of Pratylenchus smoliki n. sp. recovered from roots of six eight-week old corn hybrids. The lower bound of the box indicates the first quartile, the central line is the median, and the upper bound is the third quartile. The lower and upper whiskers represent minimum and maximum values. Outliers that are more than 1.5 times the interquartile range (third quartile minus first quartile) from the lower or upper quartile, respectively, are indicated with dots. The mean is indicated by the symbol “×”.
Figure 10:
Box and whisker plots of the distribution of numbers of Pratylenchus smoliki n. sp. recovered from roots of four eight-week old agronomic crop plants. The lower bound of the box indicates the first quartile, the central line is the median, and the upper bound is the third quartile. The lower and upper whiskers represent minimum and maximum values. Outliers that are more than 1.5 times the interquartile range (third quartile minus first quartile) from the lower or upper quartile, respectively, are indicated with dots. The mean is indicated by the symbol “×”.
Figure 11:
Box and whisker plots of the distribution of numbers of Pratylenchus smoliki n. sp. recovered from roots of 11 eight-week old cover crops. The lower bound of the box indicates the first quartile, the central line is the median, and the upper bound is the third quartile. The lower and upper whiskers represent minimum and maximum values. Outliers that are more than 1.5 times the interquartile range (third quartile minus first quartile) from the lower or upper quartile, respectively, are indicated with dots. The mean is indicated by the symbol “×”.