Figure 1:
Line drawings of Paratylenchus beltsvillensis n. sp. A: Female pharyngeal region; B: Vulval region with vulva, uterus, and spermatheca; C: Female lip region with stylet; D: Female specimen with vulval opening; E: Lateral field (mid-body); F: Male specimen with spicules and gubernaculum; G–I: Female tails with vulval opening and tail variations; J: Male tale showing spicules and gubernaculum.
Figure 2:
Photomicrographs of Paratylenchus beltsvillensis n. sp. A–E: Female anterior ends; F: Male anterior end; G–J: Female posterior ends with vulva area (arrows) and tails; K: Lateral field (mid-body); L: Male posterior end with spicules; M: Whole female specimen (arrow pointing to vulva).
Figure 3:
SEM images of Paratylenchus beltsvillensis n. sp. A: Female specimen, head; B: Lateral field (mid-body); C: Female specimen, arrow showing the vulva opening; D: Female posterior end, arrow showing the anal opening; E: Whole female specimen with arrows showing vulval and anal openings.
Table 1.
Morphometrics of Paratylenchus beltsvillensis n. sp.
| Character | Holotype | Female | Male |
|---|---|---|---|
| n | 1 | 10 | 3 |
| L | 257.0 | 252.2 ± 8.18 (245.0–267.0) | 275.0 ± 5.5 (270.0–280.0) |
| a | 20 | 18.43 ± 0.6 (18.0–19.0) | 11.0 ± 0.02 (10.98–11.02) |
| b | 2.25 | 2.12 ± 0.07 (1.99–2.23) | 3.2 ± 0.1 (3.1–3.2) |
| c | 12.85 | 11.06 ± 1.63 (9.92–13.61) | 14.43 ± 1.25 (13.0–15.3) |
| Max. body diam. | 13.0 | 13.70 ± 0.67 (13.0–15.0) | 25.0 ± 0.5 (24.5–25.5) |
| Stylet length | 70.0 | 72.25 ± 2.49 (70.0–75.0) | – |
| Ant end to Ex. Pore | 85.0 | 95.10 ± 4.72 (90.0–100.0) | – |
| Head to gland tip | 114.0 | 118.80 ± 4.10 (115.0–127.0) | 85.0 ± 5.0 (80.0–90.0) |
| Tail length | 20.0 | 23.19 ± 3.09 (17.0–27.5) | – |
| V% | 71.0 | 71.85 ± 1.07 (70.4–73.4) | – |
| Spicules | – | – | 18.3 ± 1.53 (17.0–20.0) |
| Gubernaculum | – | – | 5.17 ± 0.3 (5.0–5.5) |
Figure 4:
Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species. Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the D2-D3 of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021). *identified as Paratylenchus sp. in the GenBank.
Figure 5:
Phylogenetic relationships of Paratylenchus beltsvillensis n. sp. with other related species: Bayesian 50% majority rule consensus tree from two runs as inferred from analysis of the ITS rRNA gene sequence alignment under the GTR + I + G model. Posterior probabilities equal or more than 70% are given for appropriate clades. New sequences are indicated in bold. Clade numbers are given according to Singh et al. (2021).