Temperature: a driving factor for Meloidogyne floridensis migration toward different hosts Cover

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Temperature: a driving factor for Meloidogyne floridensis migration toward different hosts

Journal of Nematology

Volume 53 (2021): Issue 1 (January 2021)

By: Diego A. H. S. Leitão, Elvira M. R. Pedrosa, Donald W. Dickson, Ana Karina S. Oliveira and Mario Monteiro Rolim

Open Access

|Aug 2021

Figures & Tables

Experimental apparatus used in vertical migration assay of second-stage juveniles (J2) of Meloidogyne floridensis. Column consists of three 4.4-cm-d x 4-cm long rings taped together to one 2-cm long ring filled with sandy soil. Four-week-old tomato or Tagete patula plants were transplanted to 300-cm3 Styrofoam cups and taped to the upper end of the columns – cups with no plants were used as control. Freshly hatched J2 were injected through an injection hole, 1 cm above the base of the column. Arrows indicate where the 35-µm or 15-µm meshes were attached.

Distribution of recovered second-stage juveniles (J2) of Meloidogyne floridensis as a function of distance migrated and temperature at 3 (A), 6 (B), 9 (C), and 12 (D) days after injection. Nematode distribution along the column (4-cm long rings taped together) over time and at 20 and 26°C was statistically different when data were subjected to Χ
2 test (p < 0.01). — Distribution of recovered second-stage juveniles (J2) of Meloidogyne floridensis as a function of distance migrated and temperature at 3 (A), 6 (B), 9 (C), and 12 (D) days after injection. Nematode distribution along the column (4-cm long rings taped together) over time and at 20 and 26°C was statistically different when data were subjected to Χ 2 test (p < 0.01).

Percentage of active second-stage juveniles (J2) of Meloidogyne floridensis recovered at 3, 6, 9, and 12 days after injection (DAI) at 20 and 26°C from sandy soil contained in PVC columns. The columns were comprised of 4-cm long x 4.4-cm internal diameter rings. Nematode distribution over time and at both temperatures was statistically different when data were subjected to Χ
2 test (p < 0.01). — Percentage of active second-stage juveniles (J2) of Meloidogyne floridensis recovered at 3, 6, 9, and 12 days after injection (DAI) at 20 and 26°C from sandy soil contained in PVC columns. The columns were comprised of 4-cm long x 4.4-cm internal diameter rings. Nematode distribution over time and at both temperatures was statistically different when data were subjected to Χ 2 test (p < 0.01).

Distribution of active second-stage juveniles (J2) of Meloidogyne floridensis as a function of distance migrated at 3, 6, 9, and 12 days after injection (DAI). As there was no significant interaction with temperature, 20 and 26°C data were combined. Nematode distribution along the columns (4-cm long rings taped together) over time was statistically different when data were subjected to Χ
2 test (p < 0.01). — Distribution of active second-stage juveniles (J2) of Meloidogyne floridensis as a function of distance migrated at 3, 6, 9, and 12 days after injection (DAI). As there was no significant interaction with temperature, 20 and 26°C data were combined. Nematode distribution along the columns (4-cm long rings taped together) over time was statistically different when data were subjected to Χ 2 test (p < 0.01).

Penetration of second-stage juveniles (J2) of Meloidogyne floridensis on tomato or marigold over time after vertically migrating over 13 cm in sandy soil-filled PVC columns. Figures followed by the same letter do not differ according to Tukey’s multiple comparison test (p ≥ 0.05) and error bars indicate SEM.

Repeated measures MANOVA summary of the effects of plants, distance migrated (rings), temperature and time on vertical migration of recovered and active second-stage juveniles (J2) of Meloidogyne floridensis along PVC columns filled with sandy soil_

		Recovered J2				Active J2
Source	df	SS	MS	F	p > F	SS	MS	F	p > F
Block	3	166.05	55.35	20.22	<0.0001	167.94	55.98	22.24	<0.0001
Temperature (Temp)	1	60.22	60.22	22.00	<0.0001	79.11	79.11	31.43	<0.0001
Plant	2	6.72	3.36	1.23	0.2941	2.38	1.19	0.47	0.6248
Rings	4	966.36	241.59	88.26	<0.0001	696.60	174.15	69.18	<0.0001
Temp:Plant	2	1.42	0.71	0.26	0.7713	0.44	0.22	0.09	0.9160
Temp:Rings	4	13.32	3.33	1.22	0.3038	10.88	2.72	1.08	0.3650
Plant:Rings	8	15.04	1.88	0.69	0.7030	11.52	1.44	0.57	0.8012
Temp:Plant:Rings	8	33.20	4.15	1.52	0.1497	32.56	4.07	1.62	0.1181
Time	3	41.46	13.82	5.05	0.0019	64.92	21.64	8.60	<0.0001
Time:Temp	3	11.82	3.94	1.44	0.2308	28.26	9.42	3.74	0.0114
Time:Plant	6	18.66	3.11	1.14	0.3413	31.62	5.27	2.10	0.0532
Time:Rings	12	271.08	22.59	8.25	<0.0001	199.32	16.61	6.60	<0.0001
Time:Temp:Plant	6	17.70	2.95	1.08	0.3753	9.18	1.53	0.61	0.7235
Time:Temp:Rings	12	64.56	5.38	1.96	0.0266	39.36	3.28	1.31	0.2134
Time:Plant:Rings	24	72.00	3.00	1.10	0.3444	50.40	2.10	0.83	0.6935
Time:Temp:Plant:Rings	24	56.40	2.35	0.86	0.6606	62.40	2.60	1.03	0.4217

Repeated measures MANOVA summary of the effects of temperature and plants on penetration of second-stage juveniles (J2) of Meloidogyne floridensis over time_

		J2 inside roots
Source	df	SS	MS	F	p > F
Block	3	14.60	4.87	5.18	0.0037
Temperature (Temp)	1	0.19	0.19	0.20	0.6556
Plant	1	14.62	14.62	15.57	0.0003
Temp:Plant	1	2.26	2.26	2.41	0.1279
Time	3	27.89	9.30	9.90	<0.0001
Time:Temp	3	3.05	1.02	1.08	0.3663
Time:Plant	3	5.97	1.99	2.12	0.1111
Time:Temp:Plant	3	0.63	0.21	0.22	0.8800

DOI: https://doi.org/10.21307/jofnem-2021-074 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 1 - 10

Published on: Aug 12, 2021

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Peach root-knot nematode,

Related subjects:

Life sciences, other

© 2021 Diego A. H. S. Leitão, Elvira M. R. Pedrosa, Donald W. Dickson, Ana Karina S. Oliveira, Mario Monteiro Rolim, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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