Table 1.
Morphometrics of Filenchus pseudodiscus n. sp. and Golestan province population of Malenchus gilanensis (Jalalinasab et al., 2019).
| Filenchus pseudodiscus n. sp. | Malenchus gilanensis | |||
|---|---|---|---|---|
| Holotype | Paratypes | Males | Females | |
| n | 1 female | 10 females | 2 | 8 |
| L | 593 | 586 ± 22.3 (555-618) | 268, 290 | 318 ± 16.8 (296-341) |
| a | 39.5 | 37.0 ± 2.4 (30.8-39.5) | 26.8, 36.3 | 28.7 ± 3.2 (25-34) |
| b | 6.1 | 5.8 ± 0.3 (5.5-6.3) | 4.1, 3.6 | 4.0 ± 0.3 (3.6-4.3) |
| c | 4.7 | 4.7 ± 0.1 (4.5-4.8) | 5.7, 5.9 | 6.9 ± 0.5 (6.0-7.7) |
| c΄ | 12.6 | 11.8 ± 0.7 (10.9-12.8) | 4.7, 8.9 | 7.2 ± 1.1 (6-9) |
| V | 63 | 63.1 ± 0.8 (62-64) | 33.6, 29.3 | 66.8 ± 4.8 (64-76) |
| Cephalic region height | 2 | 2.4 ± 0.2 (2-3) | 2.5, 3.0 | 2.0 ± 0.5 (2-3) |
| Cephalic region width | 5 | 5.1 ± 0.3 (5-6) | 5, 5 | 5.0 ± 0.5 (4-5) |
| Stylet length | 10 | 9.6 ± 0.4 (9-10) | 7.2, 8.0 | 7.5 ± 0.5 (7-8) |
| Conus length | 3 | 3.3 ± 0.3 (3-4) | 3.5, 4.0 | 2.5 ± 0.6 (3-4) |
| m | 34 | 34.3 ± 1.6 (32-36) | 36.8, 42.5 | 37.8 ± 2.0 (35.5-41.9) |
| Dorsal gland opening | 1 | 1.4 ± 0.3 (1-2) | – | – |
| Median bulb from ant. end | 42 | 43.0 ± 2.3 (40-46) | – | – |
| MB | 43 | 42.5 ± 2.7 (39-47) | – | – |
| Excretory pore to ant. end | 80 | 80.3 ± 6.0 (72-94) | 54, 60 | 60.8 ± 3.8 (56-65) |
| Pharynx length | 98 | 101.2 ± 3.3 (97-107) | 65, 80 | 81.5 ± 3.9 (75-86) |
| Cephalic region to vulva | 375 | 370.3 ± 14.8 (350-389) | – | 211 ± 11 (197-226) |
| Body width | 15 | 15.9 ± 1.3 (15-19) | 15, 18 | 11.0 ± 1.4 (9-13) |
| Tail length | 126 | 125 ± 5 (115-133) | 47, 49 | 45 ± 5 (42-55) |
| Tail/vulva-anus | 1 | 1.4 ± 0.1 (1.2-1.5) | – | – |
| Anal body width | 10 | 10.6 ± 0.5 (10-11) | – | 7.4 ± 1.0 (6-9) |
| Post-vulval uterine sac | 8 | 10.0 ± 1.5 (8-12) | – | 6.3 ± 0.8 (5-7) |
Figure 1:
Line drawings of Filenchus pseudodiscus n. sp. (female). (A) Pharyngeal region. (B) Anterior end. (C) Entire body. (D) Cross section. (E) Reproductive system. (F, G) Tail.
Figure 2:
Light microphotographs of Filenchus pseudodiscus n. sp. (female). (A) Pharynx. (B) Anterior end showing cephalic region. (C, D) Anterior end showing cephalic region and stylet (fresh material in water). (E) Cross section at mid-body. (F) Part of female reproductive system (arrowhead showing end of PUS). (G) Tail. (H) Pharyngeal bulb region (arrowhead showing excretory pore). (A, E-H = 10 µm, B-D = 5 µm.)
Figure 3:
Scanning electron microphotographs of Filenchus pseudodiscus n. sp. (female). (A-C) Cephalic region in latero-frontal, frontal and subventral views, respectively (arrows pointing the amphidial openings). (D) Excretory pore in ventral view (arrow). (E, F) Lateral field at mid-body showing four incisures and unusual division, respectively. (G, H) Vulva in ventral and lateral views, respectively. (I) Posterior end (arrow pointing the anus). (J, K) Anus in ventral and lateral views, respectively.
Figure 4:
Light microphotographs of Golestan province population Malenchus gilanensis (Jalalinasab et al., 2019). (A) Pharynx. (B) Cephalic region and stylet. (C) Vulva region showing lateral flap. (D) Tail and spicule in lateral view. (E) Vulva region showing post-vulva uterine sac. (F) Female tail. (All scale bars = 10 μm).
Figure 5:
Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus pseudodiscus n. sp. under the GTR+G+I model. Bayesian posterior probabilities (BPP) more than 50% are given for appropriate clades. The new sequence is in bold font.
Figure 6:
Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 of Filenchus pseudodiscus n. sp. and Malenchus gilanensis under the GTR + G + I model. Bayesian posterior probabilities (BPP) more than 50% are given for appropriate clades. New sequences are in bold font.
Figure S1:
Light microphotographs of Filenchus pseudodiscus n. sp. (female). (A, B) Anterior end showing stylet. (C, D) Anterior end showing stylet and DGO. (E, F) Irregular lateral lines forming a swollen portion. (G) Lateral field at mid body showing four incisures. (All scale bars = 10 μm).