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Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran; with an updated phylogeny of Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae) Cover

Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran; with an updated phylogeny of Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae)

Open Access
|Aug 2021

Figures & Tables

Figure 1:

Line drawings of Filenchus pseudodiscus n. sp. (female). (A) Pharyngeal region. (B) Anterior end. (C) Entire body. (D) Cross section. (E) Reproductive system. (F, G) Tail.
Line drawings of Filenchus pseudodiscus n. sp. (female). (A) Pharyngeal region. (B) Anterior end. (C) Entire body. (D) Cross section. (E) Reproductive system. (F, G) Tail.

Figure 2:

Light microphotographs of Filenchus pseudodiscus n. sp. (female). (A) Pharynx. (B) Anterior end showing cephalic region. (C, D) Anterior end showing cephalic region and stylet (fresh material in water). (E) Cross section at mid-body. (F) Part of female reproductive system (arrowhead showing end of PUS). (G) Tail. (H) Pharyngeal bulb region (arrowhead showing excretory pore). (A, E-H = 10 µm, B-D = 5 µm.)
Light microphotographs of Filenchus pseudodiscus n. sp. (female). (A) Pharynx. (B) Anterior end showing cephalic region. (C, D) Anterior end showing cephalic region and stylet (fresh material in water). (E) Cross section at mid-body. (F) Part of female reproductive system (arrowhead showing end of PUS). (G) Tail. (H) Pharyngeal bulb region (arrowhead showing excretory pore). (A, E-H = 10 µm, B-D = 5 µm.)

Figure 3:

Scanning electron microphotographs of Filenchus pseudodiscus n. sp. (female). (A-C) Cephalic region in latero-frontal, frontal and subventral views, respectively (arrows pointing the amphidial openings). (D) Excretory pore in ventral view (arrow). (E, F) Lateral field at mid-body showing four incisures and unusual division, respectively. (G, H) Vulva in ventral and lateral views, respectively. (I) Posterior end (arrow pointing the anus). (J, K) Anus in ventral and lateral views, respectively.
Scanning electron microphotographs of Filenchus pseudodiscus n. sp. (female). (A-C) Cephalic region in latero-frontal, frontal and subventral views, respectively (arrows pointing the amphidial openings). (D) Excretory pore in ventral view (arrow). (E, F) Lateral field at mid-body showing four incisures and unusual division, respectively. (G, H) Vulva in ventral and lateral views, respectively. (I) Posterior end (arrow pointing the anus). (J, K) Anus in ventral and lateral views, respectively.

Figure 4:

Light microphotographs of Golestan province population Malenchus gilanensis (Jalalinasab et al., 2019). (A) Pharynx. (B) Cephalic region and stylet. (C) Vulva region showing lateral flap. (D) Tail and spicule in lateral view. (E) Vulva region showing post-vulva uterine sac. (F) Female tail. (All scale bars = 10 μm).
Light microphotographs of Golestan province population Malenchus gilanensis (Jalalinasab et al., 2019). (A) Pharynx. (B) Cephalic region and stylet. (C) Vulva region showing lateral flap. (D) Tail and spicule in lateral view. (E) Vulva region showing post-vulva uterine sac. (F) Female tail. (All scale bars = 10 μm).

Figure 5:

Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus pseudodiscus n. sp. under the GTR+G+I model. Bayesian posterior probabilities (BPP) more than 50% are given for appropriate clades. The new sequence is in bold font.
Bayesian 50% majority rule consensus tree inferred from SSU rDNA of Filenchus pseudodiscus n. sp. under the GTR+G+I model. Bayesian posterior probabilities (BPP) more than 50% are given for appropriate clades. The new sequence is in bold font.

Figure 6:

Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 of Filenchus pseudodiscus n. sp. and Malenchus gilanensis under the GTR + G + I model. Bayesian posterior probabilities (BPP) more than 50% are given for appropriate clades. New sequences are in bold font.
Bayesian 50% majority rule consensus tree inferred from LSU rDNA D2-D3 of Filenchus pseudodiscus n. sp. and Malenchus gilanensis under the GTR + G + I model. Bayesian posterior probabilities (BPP) more than 50% are given for appropriate clades. New sequences are in bold font.

Figure S1:

Light microphotographs of Filenchus pseudodiscus n. sp. (female). (A, B) Anterior end showing stylet. (C, D) Anterior end showing stylet and DGO. (E, F) Irregular lateral lines forming a swollen portion. (G) Lateral field at mid body showing four incisures. (All scale bars = 10 μm).
Light microphotographs of Filenchus pseudodiscus n. sp. (female). (A, B) Anterior end showing stylet. (C, D) Anterior end showing stylet and DGO. (E, F) Irregular lateral lines forming a swollen portion. (G) Lateral field at mid body showing four incisures. (All scale bars = 10 μm).

Morphometrics of Filenchus pseudodiscus n_ sp_ and Golestan province population of Malenchus gilanensis (Jalalinasab et al_, 2019)_

Filenchus pseudodiscus n. sp. Malenchus gilanensis
HolotypeParatypesMalesFemales
n 1 female10 females28
L 593586 ± 22.3 (555-618)268, 290318 ± 16.8 (296-341)
a 39.537.0 ± 2.4 (30.8-39.5)26.8, 36.328.7 ± 3.2 (25-34)
b 6.15.8 ± 0.3 (5.5-6.3)4.1, 3.64.0 ± 0.3 (3.6-4.3)
c 4.74.7 ± 0.1 (4.5-4.8)5.7, 5.96.9 ± 0.5 (6.0-7.7)
12.611.8 ± 0.7 (10.9-12.8)4.7, 8.97.2 ± 1.1 (6-9)
V 6363.1 ± 0.8 (62-64)33.6, 29.366.8 ± 4.8 (64-76)
Cephalic region height22.4 ± 0.2 (2-3)2.5, 3.02.0 ± 0.5 (2-3)
Cephalic region width55.1 ± 0.3 (5-6)5, 55.0 ± 0.5 (4-5)
Stylet length109.6 ± 0.4 (9-10)7.2, 8.07.5 ± 0.5 (7-8)
Conus length33.3 ± 0.3 (3-4)3.5, 4.02.5 ± 0.6 (3-4)
m3434.3 ± 1.6 (32-36)36.8, 42.537.8 ± 2.0 (35.5-41.9)
Dorsal gland opening11.4 ± 0.3 (1-2)
Median bulb from ant. end4243.0 ± 2.3 (40-46)
MB4342.5 ± 2.7 (39-47)
Excretory pore to ant. end8080.3 ± 6.0 (72-94)54, 6060.8 ± 3.8 (56-65)
Pharynx length98101.2 ± 3.3 (97-107)65, 8081.5 ± 3.9 (75-86)
Cephalic region to vulva375370.3 ± 14.8 (350-389)211 ± 11 (197-226)
Body width1515.9 ± 1.3 (15-19)15, 1811.0 ± 1.4 (9-13)
Tail length126125 ± 5 (115-133)47, 4945 ± 5 (42-55)
Tail/vulva-anus11.4 ± 0.1 (1.2-1.5)
Anal body width1010.6 ± 0.5 (10-11)7.4 ± 1.0 (6-9)
Post-vulval uterine sac810.0 ± 1.5 (8-12)6.3 ± 0.8 (5-7)
DOI: https://doi.org/10.21307/jofnem-2021-069 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 14
Published on: Aug 5, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2021 Parnaz Mortazavi, Fariba Heydari, Joaquín Abolafia, Pablo Castillo, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.