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Morphological and molecular characterisation of Tripylina gorganensis from the Slovak Republic as a contribution to the redescription of the species

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By:
Open Access
|Jun 2021

Figures & Tables

Figure 1:

Tripylina gorganensis from Slovakia. A: Male entire body; B: Anterior region (female); C: Female entire body; D: Anterior region (male); E: Cardiac region; F: Vaginal sclerotisation; G: Post-vulval uterine sac; H: Male posterior region; I: Female posterior region. (Scale bars: A, C = 50 µm; B, D–F, H = 10 µm; G = 20 µm; I = 30 µm).
Tripylina gorganensis from Slovakia. A: Male entire body; B: Anterior region (female); C: Female entire body; D: Anterior region (male); E: Cardiac region; F: Vaginal sclerotisation; G: Post-vulval uterine sac; H: Male posterior region; I: Female posterior region. (Scale bars: A, C = 50 µm; B, D–F, H = 10 µm; G = 20 µm; I = 30 µm).

Figure 2:

Tripylina gorganensis from Slovakia, female. A: Entire body; B, C: Anterior region; D–G: Vaginal sclerotisation; H: Post-vulval uterine sac; I: Posterior region. (Scale bars = 10 µm).
Tripylina gorganensis from Slovakia, female. A: Entire body; B, C: Anterior region; D–G: Vaginal sclerotisation; H: Post-vulval uterine sac; I: Posterior region. (Scale bars = 10 µm).

Figure 3:

Tripylina gorganensis from Slovakia, male. A: Entire body; B, C: Anterior region; D: Testis; E: Posterior region. (Scale bars = 10 µm).
Tripylina gorganensis from Slovakia, male. A: Entire body; B, C: Anterior region; D: Testis; E: Posterior region. (Scale bars = 10 µm).

Figure 4:

18 S rDNA-based Bayesian phylogeny of the Trischistomatidae and some other representatives of Enoplida. The new population of Tripylina gorganensis is indicated in bold. Numbers near nodes indicate posterior probabilities. ♀♂ = all species from the marked clade are dioecious; ♀ = males of the species from this clade are unknown or extremely rare.
18 S rDNA-based Bayesian phylogeny of the Trischistomatidae and some other representatives of Enoplida. The new population of Tripylina gorganensis is indicated in bold. Numbers near nodes indicate posterior probabilities. ♀♂ = all species from the marked clade are dioecious; ♀ = males of the species from this clade are unknown or extremely rare.

Figure 5:

28 S rDNA-based Bayesian phylogeny of the Trischistomatidae. The new population of Tripylina gorganensis is indicated in bold. Numbers near nodes indicate posterior probabilities. ♀♂ = all species from the marked clade are dioecious; ♀ = males of the species from this clade are unknown or extremely rare.
28 S rDNA-based Bayesian phylogeny of the Trischistomatidae. The new population of Tripylina gorganensis is indicated in bold. Numbers near nodes indicate posterior probabilities. ♀♂ = all species from the marked clade are dioecious; ♀ = males of the species from this clade are unknown or extremely rare.

1. Subventral denticles located posterior to dorsal tooth T. iandrassyi
Subventral denticles located anterior to dorsal tooth2
2. Vaginal sclerotisations oval, female without ventromedian setae in cervical region, sparse somatic setae present T. bravoae
Vaginal sclerotisations tear-shaped to semi-spherical, female with ventromedian setae in cervical region, sparse somatic setae absent3
3. Pharynx = 256–369 µm, b = 4.7–6.2 T. gorganensis
Pharynx = 216–242 µm, b = 6.3–7.4 T. longa

Morphometrics of Tripylina gorganensis from Iran and Slovakia_

IranSlovakia
CharacterFemalesMalesFemalesMales
n451614
L1,813.0 ± 53.9 (1,754–1,860) 2.971,675.0 ± 96.0 (1,558–1,790) 5.731,694.3 ± 173.1 (1,472–2,038) 10.221,662.2 ± 230.8 (1,358–2,017) 13.89
a52.6 ± 1.5 (51.6–54.7) 2.8552.5 ± 4.9 (45.7–59.7) 9.3341.0 ± 5.6 (34.4–51.5) 13.5542.2 ± 3.6 (35.8–48.5) 8.45
b6.1 ± 0.1 (5.9–6.2) 1.645.6 ± 0.3 (5.2–5.6) 5.365.4 ± 0.3 (4.9–5.8) 6.125.3 ± 0.4 (4.7–6.0) 7.71
c32.1 ± 3.2 (29.1–35.8) 9.9733.6 ± 6.5 (23.9–39.8) 19.3520.0 ± 1.8 (18.1–25.0) 8.8819.0 ± 1.9 (15.0–22.4) 9.97
c’2.3 ± 0.3 (2.0–2.6) 13.011.9 ± 0.3 (1.6–2.5) 15.792.8 ± 0.4 (2.1–3.4) 12.622.6 ± 0.2 (2.3–3.1) 7.36
V (%)79.6 ± 1.2 (78.4–81.2) 1.51 77.0 ± 1.1 (74.6–78.7) 1.47
Lip region width23.3 ± 2.6 (21–26) 11.1623.8 ± 0.8 (23–25) 3.3629.2 ± 1.3 (27.4–33.0) 4.5129.3 ± 2.3 (25.5–33.3) 7.88
Maximum body width34.5 ± 1.0 (34–36) 2.9032.0 ± 2.1 (30–35) 6.5641.6 ± 3.5 (36.3–47.8) 8.4839.3 ± 3.6 (34.7–45.3) 9.04
Anal body width24.5 ± 1.9 (23–27) 7.7626.6 ± 1.1 (25–28) 4.1430.8 ± 3.7 (25.9–35.9) 11.9634.2 ± 3.1 (29.5–39.9) 9.14
Dorsal tooth from anterior body end 27.7 ± 0.9 (25.9–29.0) 3.2927.5 ± 1.1 (25.1–29.3) 4.00
Nerve ring from anterior end 124.9 ± 9.4 (106–142) 7.54124.6 ± 13.5 (112–149) 10.85
Pharynx length297.0 ± 6.8 (288–304) 2.29297.0 ± 7.6 (286–307) 2.56316.5 ± 29.0 (256–365) 9.18315,7 ± 38.5 (256–369) 12.21
Pharynx (% of body length) 18.7 ± 1.1 (17.1–20.2) 6.1119.1 ± 1.5 (16.7–21.5) 7.82
Head to vulva1442.0 ± 58.0 (1392–1507) 4.02 1304.9 ± 132.7 (1136–1598) 10.17
Vulva to anus313.0 ± 13.1 (295–325) 4.19 303.3 ± 44.9 (255–407) 14.81
Tail length57.0 ± 6.5 (49–64) 11.4051.2 ± 9.2 (45–67) 17.9784.8 ± 5.7 (75.7–94.8) 6.7387.4 ± 7.6 (75.7–99.5) 8.67
Tail (% of body length) 5.0 ± 0.4 (4.0–5.5) 7.945.3 ± 0.6 (4.5–6.7) 10.40
Rectum/cloaca length 31.0 ± 2.2 (26.8–34.2) 7.2142.0 ± 4.9 (34.0–49.2) 11.62
Spineret length 2.1 ± 0.2 (1.8–2.3) 7.862.3 ± 0.4 (1.7–2.9) 17.20

Alphabetical list of Tripylina species and country name from which they were originally described_

SpeciesCountry
T. arenicola (de Man, 1880) Brzeski, 1963 The Netherlands
T. bravoae Cid del Prado Vera et al., 2012 Mexico
T. gorganensis Asghari et al., 2012 Iran
T. iandrassyi Cid del Prado-Vera et al., 2016 Mexico
T. ixayocensis Cid del Prado Vera et al., 2012 Mexico
T. kaikoura Zhao, 2009 New Zealand
T. longa Brzeski & Winiszewska-S´lipin´ska, 1993 Italy
T. macroseta (Vinciguerra & La Fauci, 1978) Tsalolikhin, 1983Italy
T. manurewa Zhao, 2009 New Zealand
T. montecilloensis Cid del Prado Vera et al., 2012 Mexico
T. puxianensis Xu et al., 2013 China
T. rorkabanarum Cid del Prado-Vera et al., 2016 Mexico
T. sheri Brzeski, 1963 USA (California)
T. stramenti (Yeates, 1972) Tsalolikhin, 1983New Zealand
T. tamaki Zhao, 2009 New Zealand
T. tearoha Zhao, 2009 New Zealand
T. tlamincasensis Cid del Prado Vera et al., 2012 Mexico
T. ursulae (Argo & Heyns, 1973) Tsalolikhin, 1983South Africa
T. valiathani Tahseen & Nusrat, 2010 India
T. yeatesi Zhao, 2009 New Zealand
T. ymyensis Tahseen & Nusrat, 2010 China
T. zhejiangensis Pham et al., 2013 China
DOI: https://doi.org/10.21307/jofnem-2021-048 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 10
Published on: Jun 23, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
Redescription,
Molecular,
Morphology,
Morphometrics,
Phylogeny,
Ribosomal DNA,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2021 Marek Renčo, Katarzyna Rybarczyk-Mydłowska, Łukasz Flis, Magdalena Kubicz, Grażyna Winiszewska, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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