Figure 1:
Line drawings of Prionchulus jonkershoekensis n. sp. female. (A) Head region in lateral view; (B) Entire body; (C) Neck region; (D) Pharyngo-intestinal junction (cardia); (E) Anterior genital branch with egg; (F) Posterior genital branch; (G) Rectal region and tail. (Scale bars: A, D = 20 µm; B, C = 100 µm; E, G = 50 µm; F = 10 µm).
Figure 2:
Light micrographs of Prionchulus jonkershoekensis n. sp. female. (A-B) Anterior region in lateral median view; (C) Neck region; (D) Entire body; (E) Pharyno-intestinal junction; (F) Laterial lip region showing amphidial fovea and cuticle striations; (G-H) Vagina; (I) Caudal region.
Figure 3:
Scanning electron micrographs of Prionchulus jonkershoekensis n. sp. female. (A) Lip region (Frontal view); (B) Lateral view showing labial and cephalic papillae and amphidial fovea; (C) Tail; (D) Vulva (ventral view). (L.p- Labial papillae, C.p- Cephalic papillae, Am- Amphidial fovea).
Figure 4:
A 50% majority rule Bayesian phylogenetic tree of Mononchidae, including Prionchulus jonkershoekensis n. sp. from South Africa, based on the partial 18 S rDNA sequences under the GTR + G model. The sequence of the new species is in boldface font.
Figure 5:
A 50% majority rule Bayesian phylogenetic tree of Mononchidae, including Prionchulus jonkershoekensis n. sp. from South Africa, based on the partial 28 S rDNA sequences under the GTR + G model. The sequence of the new species is in boldface font.
Table 1.
Morphometric data of Prionchulus jonkershoekensis n. sp.
| Female | ||
|---|---|---|
| Characters | Holotype | Paratypes |
| n | – | 24 |
| L | 1.84 | 1.97 ± 0.1 (1.78–2.14) |
| a | 23 | 26.9 ± 1.8 (23.0–29.7) |
| b | 3.1 | 3.3 ± 0.2 (3.0–3.5) |
| c | 11.6 | 13.0 ± 0.7 (11.6–14.3) |
| c’ | 3.3 | 3.2 ± 0.2 (2.9–3.5) |
| V | 67 | 63.3 ± 2.6 (58.6–67.4) |
| Lip region height | 9 | 9.8 ± 1.3 (8.0–12.0) |
| Lip region width | 37 | 37.0 ± 2.4 (32.0–40.0) |
| Amphid from anterior end | 14.4 | 14.6 ± 1.7 (11.2–18.0) |
| Buccal cavity length | 42 | 41.6 ± 1.4 (38.0–44.0) |
| Buccal cavity width | 26 | 27.2 ± 1.9 (24.0–31.2) |
| Position of tooth apex (%) | 29 | 28.5 ± 2.4 (25.0–33.0) |
| Nerve ring to anterior end | 195 | 184 ± 5.0 (175–195) |
| Secretory-excretory pore to anterior end | 220 | 206 ± 14.0 (185–220)* |
| Neck length | 600 | 597 ± 10.9 (580–610) |
| Cardia length | 11.2 | 12.3 ± 1.3 (10.0–15.0) |
| Cardia width | 36 | 35.0 ± 2.6 (30.0–40.0) |
| Cuticle – lip region | 2.0 | 1.8 ± 0.3 (0.8–2.4) |
| Cuticle – at vulva | 2.4 | 2.8 ± 0.9 (2.0–4.0) |
| Cuticle – at tail | 4 | 3.0 ± 1.0 (2.0–4.0) |
| Lateral chord width | 22 | 25 ± 2.4 (20.0–28.0) |
| Body diameter – neck base | 67 | 67.2 ± 2.7 (64.0–75.0) |
| Body diameter – at midbody | 80 | 73.5 ± 4.3 (66.0–82.0) |
| Body diameter – at anus | 48 | 46.6 ± 2.4 (44.0–52.0) |
| Vagina length | 26 | 31.2 ± 2.9 (26.0–35.0) |
| Rectum length | 36 | 40.1 ± 3.1 (36.0–48.0) |
| Tail length | 158 | 152 ± 4.7 (144–158) |
| Hyaline length | 6 | 6.8 ± 1.7 (4.0–8.0) |
| Egg length | 95 | 84.0 ± 7.5 (76.0–95.0)** |
| Egg width | 60 | 45.0 ± 9.0 (37.0–60.0)** |