New insights into the identity of Discolaimium dubium Das, Khan and Loof, 1969 (Dorylaimida) as derived from its morphological and molecular characterization, with the proposal of its transference to Aporcella Andrássy, 2002

Vazifeh, Nasir; Niknam, Gholamreza; Jabbari, Habibeh; Naghavi, Arezoo; Peña-Santiago, Reyes

New insights into the identity of Discolaimium dubium Das, Khan and Loof, 1969 (Dorylaimida) as derived from its morphological and molecular characterization, with the proposal of its transference to Aporcella Andrássy, 2002

Journal of Nematology

Volume 53 (2021): Issue 1 (January 2021)

By:

Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Arezoo Naghavi and Reyes Peña-Santiago

Open Access

|Jan 2021

Figures & Tables

Discolaimium dubium Das et al., 1969 from Iran. A: Neck region; B: Anterior region in lateral median view; C: Lip region in lateral surface view; D: Female entire; E: Male entire; F: Female, posterior genital branch; G: Female, posterior body region; H: Male, posterior body region; I: Spicule; J: Lateral guiding piece.

Discolaimium dubium Das et al., 1969 from Iran (female, LM). A: Anterior region in lateral median view; B: Lip region in lateral surface view; C: Pharyngeal expansion and pharyngo-intestinal junction; D: Posterior genital branch; E: Posterior body region; F: Entire; G: Vagina; H: Lateral chord. (Scale bars: A-E, G and H = 10 μm; F = 30 μm).

Discolaimium dubium Das et al., 1969 from Iran (male, LM). A: Anterior region in lateral median view; B: Entire; C: Lateral chord; D: Pharyngeal expansion and pharyngo-intestinal junction; E: Spicule; F: Posterior body region. (Scale bars: A, C-F = 10 μm; B = 30 μm).

Phylogenetic relationships of Discolaimium dubium Das et al., 1969. Bayesian 50% majority rule consensus tree as inferred from D2-D3 expansion segments of 28S rDNA sequence alignments under the GTR + G + I model. Posterior probabilities are given for appropriate clades. Newly obtained sequences are indicated by bold letters.

Main morphometrics of Iranian material of Discolaimium dubium Das et al_, 1969 and its seven close species of the genus Aporcella_

									Characters
Species	n	L	a	b	c	c′	V	Lrd^a	Odont.	Neck	Ph.exp.	Prerect.	Tail	Spicul.	Ve.sup.	Geog.dis.	Ref^b
D. dubium	20♀♀	1.10-1.39	39-49	3.5-4.7	34-43	1.6-2.0	52-58	8.0-10	7.5-10	300-350	127-159	65-109	32-38	–	–	Iran	1
	3♂♂	1.20-1.40	41-48	3.5-4.1	32-42	1.6-1.9	–	9.0-9.5	9.0-9.5	331-362	156-181	700-100	34-38	30-32	2-3
A. adriaani	7♀♀	1.23-1.49	23-30	3.5-4.1	42-58	0.8-1.1	53-57	12-15	14-16	320-380	183	63-179	21-30			South Africa	2
A. debruinae	2♀♀	2.14-2.20	40	4.2-4.5	65-76	0.9-1.0	49-50	15-16	16	480-510	–	191	29-33	–	–	Botswana	2
	2♂♂	2.12-2.23	42-43	4.4-4.8	76-93	0.8-0.9	–	16-17	14-15	460-480	–	–	24-28	57-61	5
A. malekimilani	23♀♀	1.90-2.70	32-55	4.0-5.0	41-58	1.2-1.7	55-61	14-17	14-15	476-619	300-375	105-178	44-56	–	–	Iran	3
A. minor	8♀♀	1.05-1.15	27-32	3.3-3.6	33-36	1.4-1.6	58-61	11-13	12.5-14	293-325	128-144	38-55	29-33	–	–	Iran	3
A. talebii	13♀♀	1.66-2.02	27-37	3.7-4.4	37-47	1.1-1.4	52-59	15-17	14-18	412-484	209-231	55-84	40-50	–	–	Iran	3
A. tropica	9♀♀	1.44-1.76	39-50	3.8-4.9	63-82	0.8-1.0	55-62	–	12-14	333-368	–	–	22-24	–	–	India	2
	9♂♂	1.60-1.80	44-54	4.2-4.9	60-77	1.0	–	–	–	–	–	–	22-27	42-47	3-5
A. vitrinus	32♀♀	1.20-1.86	30-47	4.0-5.1	45-77	0.9-1.4	48-53	11-13	12-13	333-374	149-185	44-132	25-37	–	–	Spain	3
	♂♂	1.47	?	?	48	1.0	–	13	12	?	?	107	31	43	6
	15♀♀	1.4-2.0	26-41	3.4-4.5	50-65	0.9-1.1	50-56	12-13	12-15	363-406	175-199	72-109	22-31	–	–	Iran

Morphometrics of Iranian material of Discolaimium dubium Das et al_, 1969_

Population	Sufiyan	Teymourlu	Qazi Jahan		Total range	Type
Character	Female	Female	Female	Male	Female	Female
n	8	6	6	3	20	25
L	1.33 ± 0.04 (1.25-1.39)	1.20 ± 0.06 (1.10-1.31)	1.20 ± 0.03 (1.10-1.30)	1.30 ± 0.08 (1.20-1.40)	1.10-1.40	1.06-1.35
a	43.0 ± 2.8 (39-48)	46.2 ± 2.2 (43-49)	44.9 ± 2.7 (41-48)	45.1 ± 2.3 (41.0-48.0)	39-49	40-49
b	3.9 ± 0.1 (3.9-4.1)	4.2 ± 0.4 (3.5-4.7)	3.9 ± 1.3 (3.8-4.1)	3.9 ± 0.3 (3.5-4.1)	3.5-4.7	3.5-4.1
c	38.0 ± 2.1 (36-43)	34.4 ± 1.3 (34.0-37.0)	38.0 ± 1.2 (37-40)	37.2 ± 3.8 (32.0-42.0)	32-43	33-39
c′	1.7 ± 0.1 (1.6-2.0)	1.8 ± 0.2 (1.8-2.0)	1.7 ± 0.04 (1.7-1.8)	1.7 ± 0.2 (1.6-1.9)	1.6-2.0	1.5-2.0
V	55.0 ± 1.5 (53-57)	54.0 ± 2.4 (52.0-58.0)	55.0 ± 0.6 (54-56)	–	52-58	53-57
Lip region diam.	9.0 ± 0.5 (8-10)	9.0 ± 0.2 (8.5-9.5)	9.2 ± 0.2 (9.0-9.5)	9.2 ± 0.1 (9.0-9.5)	8-10	10
Odontostyle length	9.4 ± 0.5 (9-10)	8.3 ± 0.7 (7.5-9.5)	9.6 ± 0.1 (9.0-10.0)	9.2 ± 0.04 (9.0-9.5)	7.5-10.5	8-10
Odontophore length	17.0 ± 1.3 (15-19)	15.7 ± 11 (14.5-17.5)	15.4 ± 0.4 (15-16)	15.6 ± 0.3 (15.5-16)	14.5-19	12-15
Guiding ring from ant. end	4.5 ± 0.3 (4-5)	5.1 ± 0.2 (5.0-5.5)	5.1 ± 0.1 (5.0-5.5)	5.0 ± 0.0 (5.0)	4-5.5	?
Neck length	334 ± 10 (318-343)	322 ± 24 (312-350)	318 ± 33 (300-332)	345 ± 15 (331-362)	300-362	320-354
Pharyngeal expansion length	156 ± 2.1 (153-159)	145 ± 12 (127-159)	139 ± 7.7 (131-147)	165 ± 14 (156-181)	127-181	?
Body diam. at neck base	28.0 ± 1.4 (26-30)	26.7 ± 1.2 (25-28)	27.5 ± 0.8 (26-29)	28.3 ± 1.6 (26-30)	25-30	?
mid-body	31.0 ± 1.6 (29-34)	26.7 ± 1.2 (25-29)	27.1 ± 1.4 (25-29)	29.5 ± 3.4 (26-33)	25-34	27
Anus	18.0 ± 0.9 (17-20)	18.7 ± 0.6 (17-19)	19.3 ± 0.9 (19-21)	21.0 ± 0.9 (20-22)	17-22	?
Prerectum length	83 ± 15 (74-103)	85.0 ± 18.3 (65-109)	80.5 ± 7.0 (73-88)	86.0 ± 12 (70-100)	65-109
Tail length	34.2 ± 1.3 (34-36)	36.2 ± 1.6 (34-38)	33.3 ± 0.6 (32-34)	36.5 ± 1.2 (34-38)	32-38	34
Spicule length	–	–	–	31.0 ± 0.9 (30-32)	–	–
Ventromedian supplements	–	–	–	(2-3)	–	–

DOI: https://doi.org/10.21307/jofnem-2021-033 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 1 - 12

Published on: Jan 1, 2021

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 times per year

Keywords:

Iran,

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© 2021 Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Arezoo Naghavi, Reyes Peña-Santiago, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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New insights into the identity of Discolaimium dubium Das, Khan and Loof, 1969 (Dorylaimida) as derived from its morphological and molecular characterization, with the proposal of its transference to Aporcella Andrássy, 2002

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Main morphometrics of Iranian material of Discolaimium dubium Das et al_, 1969 and its seven close species of the genus Aporcella_

Morphometrics of Iranian material of Discolaimium dubium Das et al_, 1969_

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