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Morphological and molecular characterization of Xiphinemella esseri Chitwood, 1957 (Dorylaimida: Leptonchidae) from Florida, with the first molecular study of the genus

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By:
Open Access
|Jan 2021

Figures & Tables

Figure 1:

Xiphinemella esseri (Chitwood, 1957) (light photomicrographs). A: Male, entire body; B: Female, entire body; C: Anterior region in dorso-ventral median view; D, F: Anterior region in lateral median view (F, arrow pointing on odontophore basal flanges); E: Lip region in surface lateral view; G: Lateral chord; H: Uterine egg; I: Pharyngeal expansion; J: Vagina; K: Anterior genital branch in part (showing the oviduct-uterus junction and uterus morphology); L: Spicule; M, O: Lateral guiding piece; N, P: Female caudal region; Q, R: Male caudal region. (Scale bars: A, B = 500 μm; C, D, F, G, I-K, N, P-R = 20 μm; E, L, M, O = 10 μm; H = 25 μm).
Xiphinemella esseri (Chitwood, 1957) (light photomicrographs). A: Male, entire body; B: Female, entire body; C: Anterior region in dorso-ventral median view; D, F: Anterior region in lateral median view (F, arrow pointing on odontophore basal flanges); E: Lip region in surface lateral view; G: Lateral chord; H: Uterine egg; I: Pharyngeal expansion; J: Vagina; K: Anterior genital branch in part (showing the oviduct-uterus junction and uterus morphology); L: Spicule; M, O: Lateral guiding piece; N, P: Female caudal region; Q, R: Male caudal region. (Scale bars: A, B = 500 μm; C, D, F, G, I-K, N, P-R = 20 μm; E, L, M, O = 10 μm; H = 25 μm).

Figure 2:

Bayesian 50% majority rule consensus tree as inferred from the D2-D3 expansion segments of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probability values more than 70% are given on appropriate clades. New sequences are indicated by bold letters.
Bayesian 50% majority rule consensus tree as inferred from the D2-D3 expansion segments of 28S rRNA gene sequence alignment under the GTR + I + G model. Posterior probability values more than 70% are given on appropriate clades. New sequences are indicated by bold letters.

Figure 3:

Bayesian 50% majority rule consensus tree as inferred from the 18S rRNA gene sequence alignment under the GTR + I + G model. Posterior probability values more than 70% are given on appropriate clades. A new sequence is indicated by bold letters.
Bayesian 50% majority rule consensus tree as inferred from the 18S rRNA gene sequence alignment under the GTR + I + G model. Posterior probability values more than 70% are given on appropriate clades. A new sequence is indicated by bold letters.

Morphometrics of Xiphinemella esseri Chitwood, 1957_

Population Florida, USA
Reference Present paper Chitwood (1957) De Bruin and Heyns (1991)
Character n 9♀♀7♂♂♀♀♂♂5♀♀4♂♂
L 2.82 ± 0.23 (2.47-3.32)2.55 ± 0.26 (2.30-3.07)2.4–3.52.2-3.03.10 (2.62-3.74)3.17 (3.06-3.25)
a 53.2 ± 3.1 (50-59)52.1 ± 6.4 (45-62)40-4335-4547.1 (39.7-58.4)53.2 (51.6-54.2)
b 8.6 (n = 1)8.2 (n = 2)11.4-14.07.6-11.010.3 (8.7-11.6)10.8 (9.9-12.2)
c 117 ± 21 (100-158)101 ± 5.5 (94-110)93-13077-120137 (95-161)124 (105-152)
c′ 0.7 ± 0.1 (0.6-0.8)0.8 ± 0.1 (0.7-0.9)0.8a 0.9a 0.6 (0.5-0.7)0.85 (0.7-1.0)
V 47.3 ± 1.5 (45.4-49.7)–42-47–46 (45-47)–
Lip region diam. 16.8 ± 0.4 (16.5-17.5)16.6 ± 0.2 (16.5-17.0)17a –17.2 (17-18)17 (16-18)
Odontostyle length 47.0 ± 1.0 (46-49)46.3 ± 0.4 (46-47)72-75b –46 (42-50)46 (45-48)
Odontophore length 35.0 ± 0.8 (34-36)34.9 ± 1.0 (33-36)––33 (31-35)34 (31-36)
Guiding ring from ant. end 36.3 ± 1.5 (34-39)36.5 ± 1.0 (35-38)35-38–36 (30-42)37 (32-42)
Neck length 288 (n = 1)288, 296 (n = 2)––300 (276-333)296 (267-322)
Pharyngeal expansion length 84.0 ± 2.8 (81-87)82.2 ± 1.9 (80-85)75-85–84 (80-89)80 (76-85)
Diam. at neck base 45.1 ± 2.8 (42-50)44.6 ± 2.5 (42-48)––––
   at midbody 53.4 ± 4.8 (49-64)50.0 ± 3.4 (46-55)––––
   at anus 34.0 ± 2.6 (32-39)30.4 ± 1.2 (29-32)––39 (30-42)31 (30-32)
Prerectum length 611, 683 (n = 2)594 ± 142,3 (444-727) (n = 3)––557 (427-695) (n = 3)–
Rectum length 36.9 ± 6.0 (27-43)45.1 ± 4.4 (40-52)––39 (36-42)–
Tail length 24.0 ± 4.0 (18-28)25.2 ± 2.0 (23-28)––23 (20-30)26 (21-31)
Spicule length –42.6 ± 1.4 (41-45)–40-45–34 (31-36)
Ventromedian supplements –9 ± 0.6 (8-10)–––8-9
DOI: https://doi.org/10.21307/jofnem-2021-032 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 9
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
18S rRNA,
28S rRNA,
Bayesian inference,
Description,
D2-D3 expansion segments,
Molecular,
Morphology,
Morphometrics,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2021 Sergio Álvarez-Ortega, Sergei A. Subbotin, Renato N. Inserra, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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