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A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Open Access
|Jan 2021

Figures & Tables

Figure 1:

Line drawing of C. tianzhuensis n. sp. A: Anterior region of second-stage juvenile; B: Head of second-stage juvenile; C, H: Cyst and cysts. D: Lateral field; E: Fenestration in vulval cone; F, G: Tail of second-stage juvenile (Scale bar: H = 500 μm; C = 200 μm; A, F, E = 20 μm; B = 10 μm; D, G = 5 μm).
Line drawing of C. tianzhuensis n. sp. A: Anterior region of second-stage juvenile; B: Head of second-stage juvenile; C, H: Cyst and cysts. D: Lateral field; E: Fenestration in vulval cone; F, G: Tail of second-stage juvenile (Scale bar: H = 500 μm; C = 200 μm; A, F, E = 20 μm; B = 10 μm; D, G = 5 μm).

Figure 2:

Light micrographs of C. tianzhuensis n. sp. A: Cyst; B: Cysts; C: Crush cyst; C: Female attached on the root (Scale bar: A, D = 500 μm; B, C = 1 mm).
Light micrographs of C. tianzhuensis n. sp. A: Cyst; B: Cysts; C: Crush cyst; C: Female attached on the root (Scale bar: A, D = 500 μm; B, C = 1 mm).

Figure 3:

Vulval cones of C. tianzhuensis n. sp. A, C: Fenestration in vulval cone (inside); B, D: Fenestration in vulval cone (outside); E, F: Cyst surface punctations. (Scale bar = 20 µm).
Vulval cones of C. tianzhuensis n. sp. A, C: Fenestration in vulval cone (inside); B, D: Fenestration in vulval cone (outside); E, F: Cyst surface punctations. (Scale bar = 20 µm).

Figure 4:

Egg of C. tianzhuensis n. sp. A: Smooth eggshell; B: Embryo egg; C: Body of developed J2 in egg. (Scale bar = 20 µm).
Egg of C. tianzhuensis n. sp. A: Smooth eggshell; B: Embryo egg; C: Body of developed J2 in egg. (Scale bar = 20 µm).

Figure 5:

Light micrographs of second stage juvenile of C. tianzhuensis n. sp. A: Entire body; B, C: Head region; D: Anterior region of J2; E: Lateral field; F: Genital primordium; G: Annulation of tail; H-I: Tail; J: Lateral field of tail. (Scale bar: A = 50 μm, B, C, D, E, F = 20 μm, H, I = 10 μm, G, J = 5 μm).
Light micrographs of second stage juvenile of C. tianzhuensis n. sp. A: Entire body; B, C: Head region; D: Anterior region of J2; E: Lateral field; F: Genital primordium; G: Annulation of tail; H-I: Tail; J: Lateral field of tail. (Scale bar: A = 50 μm, B, C, D, E, F = 20 μm, H, I = 10 μm, G, J = 5 μm).

Figure 6:

Molecular phylogenetic tree of C. tianzhuensis n. sp. (highlighted in bold) inferred from 28 S D2/D3 extension region under GTR + I + G model. The posterior probability values exceeding 50% are given on appropriate clades. *Originally identified as C. estonica in the GenBank. **Originally identified as C. rosae in the GenBank. ***Originally identified as C. estonica in the GenBank.
Molecular phylogenetic tree of C. tianzhuensis n. sp. (highlighted in bold) inferred from 28 S D2/D3 extension region under GTR + I + G model. The posterior probability values exceeding 50% are given on appropriate clades. *Originally identified as C. estonica in the GenBank. **Originally identified as C. rosae in the GenBank. ***Originally identified as C. estonica in the GenBank.

Figure 7:

Molecular phylogenetic tree of C. tianzhuensis n. sp. (highlighted in bold) inferred from ITS region under GTR + I + G model. The posterior probability values exceeding 50% are given on appropriate clades. *Originally identified as C. estonica in the GenBank. **Originally identified as C. eremica in the GenBank. ***Originally identified as C. estonica in the GenBank. ****Originally identified as C. estonica in the GenBank.
Molecular phylogenetic tree of C. tianzhuensis n. sp. (highlighted in bold) inferred from ITS region under GTR + I + G model. The posterior probability values exceeding 50% are given on appropriate clades. *Originally identified as C. estonica in the GenBank. **Originally identified as C. eremica in the GenBank. ***Originally identified as C. estonica in the GenBank. ****Originally identified as C. estonica in the GenBank.

Morphological and morphometrics of characters of cysts, eggs and J2s, useful for identification of Cactodera species_

Stage
CystSecond-stage juvenileEgg
SpeciesLengthWidthL/W ratioVulval denticlesFenestral diam.LengthDGOStylet LengthTail LengthHyaline tail lengthEggshell surfaceOriginal des.
C. acnidae 504–857319–4931.2–2.430 × 33361–4482.5–3.019–2543–4817–26Smooth Schuster and Brezina (1979)
C. amaranthi 525–774370–5501.1–1.7Present25–38340–4603.9–5.120–2132–4012–16Smooth Golden and Raski (1977)
C. cacti 389–658323–5981.0–1.4Present16–30456–5402.9–4.424–2649–6014–21Punctate Graney and Bird (1990)
C. chenopodiae 423–585283–3981.2–1.7Absent20–26438–5393.1–4.322–2639–5117–28Punctate Feng et al. (2018)
C. eremica 530–810290–5901.2–1.9Absent14–25440–5103.5–6.025–2836–4717–23Punctate Baldwin and Bell (1985)
C. estonica 686–1014312–4682.0–2.4Present18–30426–4653.4–4.322–2436–4414–21Smooth Golden and Raski (1977)
C. evansi 416–528284–3841.2–1.718–23358–4202.8–4.020–2434–4416–23Punctate Cid Del Prado and Rowe (2000)
C. galinsogae 453–675284–5081.1–1.7Absent33–56358–44319–3126–4510–24Punctate Soto et al. (2003)
C. milleri 550–849419–5981.2–1.6Present14–18370–4793.2–5.121–2337–4915–21Punctate Graney and Bird (1990)
C. radicale 553–986220–6261.3–2.6Absent17–28467–5203.6–5.820–2746–6015–28Smooth Subbotin et al. (2010)
C. rosae 460–840280–5601.2–2.1Present10–21348–47216–2631–684–8Punctate Cid Del Prado and Miranda (2008)
C. salina 415–742193–4751.4–2.2Absent20–28410–5142.5–4.023–2531–4810–31Smooth Baldwin et al. (1997)
C. solani 291–581204–5051.2–1.4Present20–36379–5113.7–6.924–2728–4912–23Punctate Escobar-Avila et al. (2020)
C. thornei 485–806286–5811.2–1.9Present31–36446–6205–725–2849–6423–28Punctate Golden and Raski (1977)
C. tianzhuensis n. sp. 511–761 361–558 1.1–1.6 Present 20–32 495–592 4.0–6.5 23–26 47–59 22–29 Smooth In this study
C. torreyanae 364–71292–4321.4–2.9Absent20×26390–5502–421–2332–4516–25Smooth Cid Del Prado and Subbotin (2014)
C. weissi 524–598350–3941.2–2.3Present29–38407–4894.5–5.620–2243–5017–24Smooth Golden and Raski (1977)

Morphometrics of C_ tianzhuensis n_ sp_

StageCharacterHolotypeParatype
Cyst
n 20
L (excluding length)531571.2 ± 79.1 (511.0–761.0)
Diam.429454.3 ± 56.8 (360.5–558.0)
L/Diam.1.231.3 ± 0.1 (1.1–1.6)
Fenestral diam.22.823.4 ± 3.8 (20.0–31.5)
Second-stage juvenile
n 20
Body length 538.5 ± 25.0 (494.5–591.5)
Body width at mid-body 23.2 ± 1.4 (20.0–25.0)
a 23.4 ± 2.0 (20.6–27.2)
b 3.7 ± 0.3 (3.1–4.2)
c 10.0 ± 0.8 (8.4–11.6)
c′ 3.9 ± 0.3 (3.5–4.5)
Lip region height 4.8 ± 0.4 (4.0–5.0)
Lip region diam. 10.7 ± 0.8 (9.0–12.0)
Stylet length 24.9 ± 0.8 (22.5–26.0)
Stylet base height 2.5 ± 0.3 (2.0–3.0)
Stylet base width 5.1 ± 0.54 (4.0–5.5)
Median bulb from anterior end (MB) 77.8 ± 4.2 (69.5–85.0)
Opening of dorsal pharyngel gland from stylet base (DGO) 4.9 ± 0.5 (4.0–6.5)
Excretory pore from anterior end (EP) 111.2 ± 6.2 (97.5–124.0)
Median bulb width (MBW) 11.8 ± 1.1 (9.5–13.5)
Diam. at anus 13.8 ± 0.5 (11.5–15.0)
Tail length 54.1 ± 3.6 (46.5–59.0)
Hyaline portion tail 25.8 ± 2.1 (21.5 –28.5)
L/MB 6.9 ± 0.5 (6.1–8.0)
TL/H 2.1 ± 0.2 (1.8–2.4)
Egg
n 20
Length 117.9 ± 11.6 (101.5–144.0)
Width 51.3 ± 4.1 (41.0–61.5)
Length/Width 2.3 ± 0.3 (1.9–2.9)
DOI: https://doi.org/10.21307/jofnem-2021-029 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 15
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2021 Wenhao Li, Huixia Li, Chunhui Ni, Mingming Shi, Xuejuan Wei, Yonggang Liu, Yiwen Zhang, Deliang Peng, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.