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Basilaphelenchus hyrcanus n. sp. (Rhabditida: Tylaphelenchinae) associated with bark of a beech tree (Fagus orientalis Lipsky) from northern Iran

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By:
Open Access
|Jan 2021

Figures & Tables

Figure 1:

Line drawing of Basilaphelenchus hyrcanus n. sp. (A) Female entire body – (B) Male entire body – (C) Female head – (D) Vulval region – (E) Anterior body – (F) Male posterior region in lateral view showing genital papillae (P2-P4) − (G) Female posterior region (Scale bars: A-C and E, F = 10 μm; D, G = 20 μm.)
Line drawing of Basilaphelenchus hyrcanus n. sp. (A) Female entire body – (B) Male entire body – (C) Female head – (D) Vulval region – (E) Anterior body – (F) Male posterior region in lateral view showing genital papillae (P2-P4) − (G) Female posterior region (Scale bars: A-C and E, F = 10 μm; D, G = 20 μm.)

Figure 2:

Photomicrographs of Basilaphelenchus hyrcanus n. sp. (A-C) Female anterior body – (D) Part of pharynx– (E-G) Metacorpus region showing excretory pore (arrowhead) – (H) Lateral field – (I) Male posterior body showing spicules and papillae arrangement (P2 + P4 arrowheads) – (J, K) Vulval region showing post-vulval uterine sac (arrowhead) – (L, M) Female tail (Scale bars = 10 μm.)
Photomicrographs of Basilaphelenchus hyrcanus n. sp. (A-C) Female anterior body – (D) Part of pharynx– (E-G) Metacorpus region showing excretory pore (arrowhead) – (H) Lateral field – (I) Male posterior body showing spicules and papillae arrangement (P2 + P4 arrowheads) – (J, K) Vulval region showing post-vulval uterine sac (arrowhead) – (L, M) Female tail (Scale bars = 10 μm.)

Figure 3:

Bayesian 50% majority rule consensus tree inferred from the small subunit (SSU) rDNA gene sequences of Basilaphelenchus hyrcanus n. sp. under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values greater than 0.50 and 50, respectively, are given for appropriate clades in the pattern of BPP/ML BS. The new species taxon is represented in bold.
Bayesian 50% majority rule consensus tree inferred from the small subunit (SSU) rDNA gene sequences of Basilaphelenchus hyrcanus n. sp. under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values greater than 0.50 and 50, respectively, are given for appropriate clades in the pattern of BPP/ML BS. The new species taxon is represented in bold.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from the D2-D3 large subunit (LSU) rDNA gene sequences of Basilaphelenchus hyrcanus n. sp. under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values greater than 0.50 and 50, respectively, are given for appropriate clades in the pattern of BPP/ML BS. The new species taxon is represented in bold.
Bayesian 50% majority rule consensus tree inferred from the D2-D3 large subunit (LSU) rDNA gene sequences of Basilaphelenchus hyrcanus n. sp. under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values greater than 0.50 and 50, respectively, are given for appropriate clades in the pattern of BPP/ML BS. The new species taxon is represented in bold.

Morphometric data for Basilaphelenchus hyrcanus n_ sp_ (measurements μm; mean ± s_d_ and (range) for paratypes)_

FemaleMale
CharacterHolotypeParatypesParatypes
n–107
L350383 ± 34.0 (316–418)370 ± 15.1 (350–395)
a32.037.0 ± 3.5 (31.6–41.8)40.0 ± 2.2 (37.4–44.0)
b7.68.3 ± 0.7 (7.3–9.5)7.3 ± 0.2 (7.0–7.6)
b′3.64.0 ± 0.2 (3.5–4.1)3.6 ± 0.3 (3.3–4.2)
c12.113.0 ± 0.9 (11.5–14.0)13.6 ± 0.5 (12.8–14.2)
c′4.85.4 ± 0.5 (4.8–6.2)3.8 ± 0.4 (3.1–4.3)
Vc or T71.469.7 ± 3 (63.0–75.5)57.3 ± 3.1 (53.5–60.8)
Lip region height2.02.0 ± 0.2 (2.0–2.5)2.1 ± 0.2 (2.0–2.5)
Lip region width5.04.8 ± 0.4 (4.0–5.0)4.9 ± 0.4 (4.0–5.0)
Stylet length9.09.4 ± 0.5 (9.0–10.0)9.3 ± 0.5 (9.0–10.0)
Conus length4.03.4 ± 0.5 (3.0–4.0)3.4 ± 0.5 (3.0–4.0)
m1 44.036.0 ± 3.4 (34.0–40.0)36.0 ± 2.7 (34.0–44.0)
Maximum body diam.11.010.3 ± 0.5 (10.0–11.0)9.3 ± 0.5 (9.0–10.0)
MB2 80.085.0 ± 1.8 (83.3–88.0)86.5 ± 1.3 (84.6–88.2)
Median bulb width6.06.2 ± 0.6 (5.0–7.0)6.3 ± 0.5 (6.0–7.0)
Median bulb length10.010.7 ± 0.7 (10.0–12.0)11.1 ± 0.4 (11.0–12.0)
Median bulb length/diam. Ratio1.71.7 ± 0.1 (1.6–2.0)1.8 ± 0.2 (1.6–2.0)
Nerve ring from anterior body54.051.1 ± 4.1 (45.0–55.0)56.0 ± 1.8 (55.0–60.0)
Excretory pore from anterior end44.044.1 ± 2.8 (40.0–48.0)48.6 ± 1.3 (46.0–50.0)
Ovary or Testis length130138 ± 10.3 (125–155)212 ± 16.3 (190–240)
Post-uterine sac42.045.3 ± 7.0 (38.0–60.0)–
Vulva to anus distance88.089.0 ± 9.2 (68.0–98.0)–
Post-uterine sac length/vulva to anus (%)47.751.4 ± 9.3 (42.1–70.6)–
Vulval body diam.10.09.3 ± 0.7 (8.0–10.0)–
Anal (cloacal) body diam.6.05.6 ± 0.5 (5.0–6.0)7.3 ± 0.5 (7.0–8.0)
Tail length29.030.0 ± 1.1 (28.0–31.0)27.3 ± 2.0 (25.0–30.0)
Spicule length (arc)––10.3 ± 0.5 (10.0–11.0)
DOI: https://doi.org/10.21307/jofnem-2021-028 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 11
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
LSU,
Golestan province,
Molecular,
Morphology,
New species,
Phylogeny,
SSU,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2021 Behrouz Golhasan, Esmaeil Miraeiz, Zahra Tanha maafi, Ramin Heydari, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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