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Performance of entomopathogenic nematodes on the mealybug, Dysmicoccus brevipes (Hemiptera: Pseudococcidae) and the compatibility of control agents with nematodes

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By:
Open Access
|Jan 2021

Figures & Tables

Figure 1:

Mortality (%) from Dysmicoccus brevipes adult female (mean ± SD) caused by entomopathogenic nematodes applied in different concentrations in laboratory conditions (25 ± 1°, RH 70 ± 10% and without photophase). *Regression equations: NEPET11: y = −0.006 x 2 + 1,5478x + 22,985. R 2 = 0.732; IBCB40: y = 0.0045 x 2 + 12,568x + 15.6. R 2 = 0.8257.
Mortality (%) from Dysmicoccus brevipes adult female (mean ± SD) caused by entomopathogenic nematodes applied in different concentrations in laboratory conditions (25 ± 1°, RH 70 ± 10% and without photophase). *Regression equations: NEPET11: y = −0.006 x 2 + 1,5478x + 22,985. R 2 = 0.732; IBCB40: y = 0.0045 x 2 + 12,568x + 15.6. R 2 = 0.8257.

Figure 2:

Mortality (%) from Dysmicoccus brevipes adult female (mean ± SD) caused by Heterorhabditis amazonensis (NEPET11) at different depths of sand column, under laboratory conditions.
Mortality (%) from Dysmicoccus brevipes adult female (mean ± SD) caused by Heterorhabditis amazonensis (NEPET11) at different depths of sand column, under laboratory conditions.

Figure 3:

Mortality (%) of Dysmicoccus brevipes adult females (mean ± SD) caused by Heterorhabditis amazonensis (NEPET11) in a circular arena for observation of horizontal displacement.
Mortality (%) of Dysmicoccus brevipes adult females (mean ± SD) caused by Heterorhabditis amazonensis (NEPET11) in a circular arena for observation of horizontal displacement.

Compatibility of the entomopathogenic nematode Heterohabditis amazonensis (NEPET11) exposed for 48 hours to phytosanitary products registered or in the process of registration for cassava culture (IOBC/WPRS Protocol) (Vainio, 1992)_

DataTreatmentViability (%)a Infectivity (%)MC%Rinf%E%Classificationb
28/05/2015Control92.2 ± 0.6 aa 92 ± 3.7 a––––
Curyom 550 EC90.8 ± 1.2 a8 ± 3.7 b1.5291.3092.82Moderately toxic
Poquer79.8 ± 3.1 b90 ± 6.3 a13.452.1715.62Innocuous
20/06/2015Control99.4 ± 0.2 a86 ± 5.1 a––––
Fusilade 250 EW99.2 ± 0.4 a62 ± 8.6 a0.2027.9128.11Innocuous
Cipermetrina 250 EC97.4 ± 0.6 b80 ± 8.9 a2.016.988.99Innocuous
Gamit 360 CS99.0 ± 0.3ab74 ± 9.3 a0.4013.9514.36Innocuous
Nomolt® 15099.2 ± 0.2 a66 ± 6.8 a0.2023.2623.46Innocuous
Callisto98.0 ± 0.5 ab90 ± 5.5 a1.40.001.41Innocuous
24/07/2015Control92.8 ± 0.9 a86 ± 2.4 a––––
Tiger 100 EC76.6 ± 2.5 b76 ± 2.4 a17.4611.6329.08Innocuous
Standak top®87.2 ± 1.7 a72 ± 4.9 a6.0316.2822.31Innocuous
Actara 250 WG70.8 ± 1.9 b86 ± 5.1 a23.710.0023.71Innocuous
Gaucho FS71.2 ± 2.5 b86 ± 2.4 a23.280.0023.28Innocuous

Isolates, species, and collection sites of Heterorhabditis amazonensis and Heterorhabditis indica used in the selection test for adult females of Dysmicoccus brevipes in laboratory conditions_

IsolateSpeciesReferenceCollection location
RSC05 H. amazonensis Andaló et al. (2006) Benjamin Constant – AM – Brazil
GL (Alho) H. amazonensis Andaló et al. (2009) Lavras – MG – Brazil
JPM4 H. amazonensis Molina et al. (2005), Andaló et al. (2014) Lavras – MG – Brazil
RSC 03 H. amazonensis Andaló et al. (2006) Benjamin Constant – AM – Brazil
JPM3 H. amazonensis Molina et al. (2005), Andaló et al. (2014) Lavras – MG – Brazil
IBCB-n40 H. amazonensis Dell’Acqua et al. (2013) Taboporã – SP – Brazil
IBCB-n46 H. amazonensis Dell’Acqua et al. (2013) Santo Antônio de Posse – SP– Brazil
IBCB-n44 H. amazonensis Dell’Acqua et al. (2013) Santa Adélia – SP – Brazil
NEPET11 H. amazonensis Ribeiro (2018) Palmeira das Missões – RS– Brazil
IBCB-n05 H. indica Dell’Acqua et al. (2013) Itapetininga – SP – Brazil

Trade name, biological activity, active ingredient, and dose of commercial product of phytosanitary products registered or in the process of registration for cassava culture, evaluated for compatibility with Heterohabditis amazonensis (isolated NEPET11) (AGROFIT, 2020)_

Commercial nameBiological activityActive ingredientSituationDose/Haa Dose/Lb
Curyom 550 ECInsecticideLufenuron 50 g/L + profenofos 500 g/LRegistered300 mL2.08 mL
Gaucho FSInsecticideImidacloprid 600 g/LNot registered360 mL2.50 mL
Actara 250 WGInsecticideThiamethoxam 250 g/kgNot registered150 g1.04 g
Tiger 100 ECInsecticidePyriproxyfen 100 g/LNot registered220 g2.50 g
Nomolt 150InsecticideTeflubenzuron 150 g/LRegistered300 mL1.50 mL
Cipermetrina 250ECInsecticideCypermethrin 250.0 g/LRegistered50 mL2.08 mL
Standak TopInsecticide FungicidePyraclostrobin 25 g/L + Thiophanate-methyl 225 g/L + Fipronil 250 g/LNot registered360 mL0.69 mL
PoquerHerbicideClethodim 240g/LRegistered400 mL2.77 mL
Fusilade 250 EWHerbicidefluazifop-butyl 250 g/LRegistered700 mL4.84 mL
Gamit 360 CSHerbicideClomazone 360.0 g/LRegistered2,000 mL13.83 mL
CallistoHerbicideMesotrione 480.0 g/LNot registered400 mL2.77 mL

Mortality (%) of Dysmicoccus brevipes female (mean ± SD) caused by isolates of Heterorhabditis in laboratory conditions (25 ± 1°, UR 70 ± 10% and without photophase)_

IsolateSpecies% Mortality
Control–0.0 ± 0.0 aa
RSC05 H. amazonensis 46.0 ± 6.0 bc
IBCB-n46 H. amazonensis 48.5 ± 10.3 bc
IBCB-n44 H. amazonensis 48.9 ± 20.1 bc
IBCB-n05 H. indica 52.5 ± 6.5 bc
JPM4 H. amazonensis 54.0 ± 8.7 bc
JPM3 H. amazonensis 54.7 ± 6.3 bc
RSC 03 H. amazonensis 59.4 ± 7.4 cd
GL (Alho) H. amazonensis 61.0 ± 14.8 cd
IBCB-n40 H. amazonensis 84.0 ± 8.1 de
NEPET11 H. amazonensis 93.8 ± 4.1 e
DOI: https://doi.org/10.21307/jofnem-2021-020 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 10
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Biological control,
Pineapple mealybug
Related subjects:
Life sciences,
Life sciences, other

© 2021 Marcelo Zart, Mariana Ferracim de Macedo, Jael Simões Santos Rando, Gabriela Souza Doneze, Cassia Pereira Brito, Rodrigo de Souza Poletto, Viviane Sandra Alves, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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