Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Phougeishangbam Rolish Singh; Gerrit Karssen; Kelvin Gitau; Cecilia Wanjau; Marjolein Couvreur; Njira Njira Pili; Godelieve Gheysen; Wim Bert

doi:10.21307/jofnem-2021-016

Figures & Tables

Light microscopy and scanning electron microscopy images of Rotylenchus wimbii n. sp. female. A to C: En face view; D to I: Anterior part of the body showing lip and neck region; J: Whole female body; K to N: Vulva region; O to V: Tail region.

Illustrations of Rotylenchus wimbii n. sp. female. A, B: Anterior part of the body showing lip and neck region; C: En face view; D, E: Lip region; F: Whole body; G to J: Tail region; K: Vulva region. Scales are given in µm.

BI phylogenetic tree generated from the analysis of D2-D3 of 28S rDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.

BI phylogenetic tree generated from the analysis of ITS of rDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.

BI phylogenetic tree generated from the analysis of COI of mtDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.

Morphometric data for fixed female Rotylenchus wimbii n_ sp_ specimens mounted in glycerin_

Female character
n	19
Body length (L)	690 ± 44 (620-760)
a = L/MBD	27.3 ± 3.0 (23.0-33.0)
b = L/Anterior end to pharynx-intestine junction	5.8 ± 0.3 (5.0-6.0)
b′ = L/Anterior end to end of pharyngeal gland	5.1 ± 0.4 (4.5-6.1)
c = L/Tail length	66.0 ± 12.0 (49.0-91.0)
c′ = Tail length/ABD	0.6 ± 0.1 (0.3-0.8)
V = Anterior end to vulva opening/L × 100	58 ± 1 (55-60)
Lip region height	4.6 ± 0.3 (4.2-5.3)
Lip region width	8.2 ± 0.3 (7.7-8.7)
Stylet length	25.3 ± 0.9 (22.5-26.6)
Cone length	12.5 ± 0.7 (10.8-13.7)
Shaft length	10.3 ± 0.5 (9.4-11.2)
Stylet knob height	2.6 ± 0.4 (1.9-3.4)
Cone % Stylet	49.3 ± 1.8 (44.8-52.6)
Dorsal gland opening from stylet base	5.4 ± 0.6 (4.1-6.3)
Anterior end to secretory-excretory pore	107 ± 6.2 (93.2-118)
Anterior end to pharynx-intestine junction	118 ± 5.3 (105-128)
Anterior end to pharyngeal gland end	135 ± 6.0 (121-144)
Anterior end to vulva opening	400 ± 24.8 (341-408)
Maximum body diameter (MBD)	25.5 ± 2.9 (21.5-31.7)
Anal body diameter (ABD)	16.9 ± 1.4 (15.2-19.9)
Tail length	10.8 ± 2.1 (6.9-13.4)
Phasmid to tail tip length	31.6 ± 3.1 (25.1-36.3)
Number of annuli between phasmid and tail tip	20 ± 3 (12-26)
Number of tail annuli	7 ± 1 (5-9)

Comparison of some important female characters and information on availability of males of 23 Rotylenchus species reported from Africa_

Species	Body length (mm)	Lip region	Lip annuli number	Stylet length (µm)	Cone% of stylet	Spermatheca in matured females	V%	Male	Tail annuli number	Phasmid position
R. abnormecaudatus	0.6-0.7	Broadly rounded, setoff to continuous	4-5	24-25	42-45	Not seen	56-59	Not found	8-11	8-14 annuli anterior to anus
R. acuspicaudatus	0.8-1.0	Broadly rounded, slightly setoff	4-5	26-28	43-51	Rounded, filled with sperms	54-57	Found	13-16	7-15 annuli anterior to anus
R. alius	0.5-0.9	Truncate, blunt anterior, not setoff	4-5	20-31	42-51	Rounded, filled with sperms	54-66	Found	8-15	3-4 annuli posterior to anus
R. bialaebursus	0.6-0.8	Rounded, not setoff	4	26-29	46-50	Rounded, filled with sperms	54-59	Found	6-8	9-12 annuli anterior to anus
R. brevicaudatus	0.5-0.8	Broad, rounded, not setoff	4-5	18-26	–	Present	52-62	Found	5-11	8-14 annuli anterior to anus
R. capensis	0.7-1.2	Hemispherical, slightly setoff	5-7	25-35	39-45	Rounded, filled with sperms	54-59	Found	9-14	1-3 annuli anterior to anus
R. catharinae	0.8-1.1	Hemispherical, slightly setoff	7-8	30-33	45-48	Rounded, filled with sperms	55-56	Found	13-14	1-4 annuli anterior to anus
R. caudaphasmidius	0.6-0.9	Hemispherical, not setoff	5	24-32	–	Rounded, filled with sperms	55-63	Found	12	5 annuli posterior to anus
R. cypriensis	0.5-0.8	Hemispherical, well setoff	3-4	21-28	–	Small and empty	57-67	Not found	6-7	7-14 annuli anterior to anus
R. devonensis	0.7-0.9	Conoid, blunt, not setoff	4	25-34	43-46	Rounded, filled with sperms	53-64	Found	7-9	8-14 annuli anterior to anus
R. wimbii n. sp.	0.6-0.8	Hemispherical, not setoff	4	23-27	45-53	Indistinct to empty	55-60	Not found	5-9	7-17 annuli anterior to anus
R. gracilidens	0.9-1.2	Hemispherical, slightly setoff	4	29-35	–	Rounded, filled with sperms	53-59	Found	8-12	15-20 annuli anterior to anus
R. incultus	0.6-1.0	Hemispherical, not setoff	4-5	22-32	–	Developed, filled with sperms	53-61	Found	ca 8	13-14 annuli anterior to anus
R. karooensis	0.7-1.0	Rounded, slightly setoff	4-5	21-27	43-47	Rounded, filled with sperms	51-60	Found	7-15	1-6 annuli anterior to anus
R. kenti	0.5-0.7	Rounded, not setoff	4-5	21-25	44-52	Rounded, filled with sperms	60-66	Found	9-17	3-4 annuli anterior to anus
R. mabelei	0.9-1.1	Hemispherical, slightly setoff	4-5	27-30	43-50	Empty	48-57	Not found	14-18	3 annuli anterior to 5 annuli posterior of anus
R. minutus	0.5-0.8	Angularly hemispherical, slightly setoff	3-4	20-27	56-58	Rounded, filled with sperms	53-66	Found	9-14	1 annule anterior to 4 annuli posterior of anus
R. mirus	0.6-0.7	Hemispherical, setoff	4-5	20-23	43-50	Rounded, filled with sperms	55-58	Found	6-10	5-12 annuli anterior to anus
R. robustus	0.9-1.6	Hemispherical, well setoff	6-7	33-50	50-56	Rounded, filled with sperms	52-58	Found	8-17	7 annuli anterior to 3 annuli posterior of anus
R. rugatocuticulatus	1.0-1.3	Rounded, slightly setoff	4	35-42	–	Developed, filled with sperms	51-56	Found	12	8-10 annuli anterior to anus
R. triannulatus	0.6-0.8	Rounded, well setoff	3	24-25	43-48	Rounded, filled with sperms	56-60	Found	10-11	Anal level to 8 annuli anterior
R. unisexus	0.5-0.9	Hemispherical, not setoff	4	20-29	–	Not seen	47-59	Not found	9-13	6-7 annuli anterior to anus
R. usitatus	1.1-1.2	Flattened anteriorly, not setoff	4-5	29-29	43-47	Rounded, filled with sperms	45-55	Found	10-16	8-14 annuli anterior to anus

Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Figures & Tables

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Morphometric data for fixed female Rotylenchus wimbii n_ sp_ specimens mounted in glycerin_

Comparison of some important female characters and information on availability of males of 23 Rotylenchus species reported from Africa_

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