Table 1.
The used sequences in 28S phylogeny of Longidorella (Saevadorella) caspica n. sp., their accession number, locality, and related plants.
| Species name | Accession number | Location, related plant |
|---|---|---|
| Allodorylaimus sp. | KY942069 | China, unknown |
| Allodorylaimus andrassyi | AY593016 | Unknown |
| Allodorylaimus andrassyi | AY593015 | Unknown |
| Dorydorella bryophila | HM235514 | Unknown |
| Enchodelus sp. | KP190120 | Hamedan province, Iran, mosses |
| Enchodelus longispiculus | KP190119 | Hamedan province, Iran, mosses |
| Enchodelus sp. | EF207240 | Unknown |
| Enchodorus dolichurus | KR184125 | Golestan province, Iran, mosses |
| Enchodorus dolichurus | KR184124 | Golestan province, Iran, mosses |
| Enchodorus yeatsi | KX691911 | East Azarbaijan province, Iran, grasses |
| Eudorylaimus sp. | AY593037 | Unknown |
| Heterodorus brevidentatus | KP963962 | Kerman city, Iran, mosses |
| Heterodorus brevidentatus | KP963960 | Maragheh city, Iran, mosses |
| Heterodorus brevidentatus | KP963963 | Maragheh city, Iran, mosses |
| Heterodorus brevidentatus | KP963964 | Kermanshah city, Iran, mosses |
| Heterodorus brevidentatus | KP963965 | Kermanshah city, Iran, mosses |
| Heterodorus brevidentatus | KP963961 | Tehran city, Iran, mosses |
| Heterodorus morgensis | KX691912 | Mazandaran province, Iran, grasses |
| Heterodorus youbertghostai | KR184126 | Sabalan region, Iran, grasses |
| Heterodorus youbertghostai | KR184127 | Sabalan region, Iran, grasses |
| Longidorella macramphis | AY593042 | Unknown |
| Longidorella sp. | AY593043 | Unknown |
| Longidorella penetrans | HM235515 | Unknown |
| Longidorella sp. | AY593044 | Unknown |
| Longidorella sp. | AY593045 | Unknown |
| Mesodorylaimus sp. | AY593006 | Unknown |
| Mesodorylaimus sp. | AY593005 | Unknown |
| Microdorylaimus miser | AY593046 | Unknown |
| Microdorylaimus modestus | HM235513 | Unknown |
| Microdorylaimus modestus | AY593049 | Unknown |
| Mononchus truncatus | AY593064 | Unknown |
| Nordiidae sp. | KP202362 | Tehran city, Iran, grasses |
| Nordiidae sp. | KP202361 | Tehran city, Iran, grasses |
| Nordiidae sp. | AY593054 | Unknown |
| Nordiidae sp. | MH346478 | Tehran city, Iran, mosses/pine tree |
| Prionchulus punctatus | MG994945 | Unknown |
| Prodorylaimus uliginosus | AY593034 | Unknown |
| Pungentus engadinensis | MH346473 | Semnan province, Iran, fruit trees |
| Pungentus engadinensis | MH346474 | Mazandaran province, Iran, forest trees |
| Pungentus silvestris | AY593052 | Unknown |
| Pungentus silvestris | AY593054 | Unknown |
| Pungentus silvestris | AY593053 | Unknown |
| Pungentus azarbaijanensis | MH346476 | West Azarbaijan province, Iran, grasses |
| Pungentus azarbaijanensis | MH346477 | West Azarbaijan province, Iran, grasses |
| Pungentus engadinensis | AY593050 | Unknown |
| Pungentus monohystera | MF325344 | Germany, lime tree |
| Pungentus monohystera | MF325343 | Germany, lime tree |
| Rhyssocolpus vinciguerrae | KP204547 | Gilan province, Iran, forest trees |
| Thonus minutus | AY593047 | Unknown |
| Thonus minutus | AY593048 | Unknown |
| Thonus circulifer | AY593039 | Unknown |
| Thonus sp. | AY593041 | Unknown |
| Thonus sp. | AY593040 | Unknown |
| Thonus circulifer | AY593038 | Unknown |
| Tylencholaimellus sp. | AY593055 | Unknown |
Figure 1:
Line drawings of Longidorella (Saevadorella) caspica n. sp. A-C, E-G: Female; D: Male. A: Pharynx; B: Anterior body region; C: Anterior end; D: Posterior body region; E and G: Tail and posterior body region, F: Posterior genital tract.
Figure 2:
Photomicrographs of Longidorella (Saevadorella) caspica n. sp. A-D, F-I: Female; E: Male. A-D: Anterior and cephalic region; E and F: Entire body; G: Vulva in ventral view; H: Mature egg inside female reproductive system; I: Sperm inside the uterus. (Scale bars = 10 μm, except E and F = 100 μm).
Figure 3:
Photomicrographs of Longidorella (Saevadorella) caspica n. sp. A and B, F and G: Male; C-E: Female. A and B: Posterior body region (Arrows showing supplements). C. Pharyngeal bulb. F-G: Posterior body region with spicules. (All scale bars = 10 μm).
Table 2.
Morphometrics of Longidorella (Saevadorella) caspica n. sp.
| Holotype | Paratype | ||
|---|---|---|---|
| Characters | Female | Females | Males |
| n | 1 | 3 | 3 |
| L | 852 | 832 ± 38.5 (788-874) | 811 ± 74 (725-856) |
| a | 19.8 | 19 ± 2 (16.8-21.3) | 22.0 ± 1.5 (20.7-23.8) |
| b | 3.8 | 3.7 ± 0.2 (3.5-3.8) | 3.8 ± 0.2 (3.6-4.0) |
| c | 25.8 | 27.2 ± 7 (20.7-37.0) | 25.2 ± 2.2 (23.0-27.5) |
| c′ | 1.3 | 1.3 ± 0.3 (0.9-1.5) | 1.3 ± 0.1 (1.2-1.4) |
| V | 59.2 | 55.7 ± 3.0 (52.5-59.0) | – |
| Anterior end-vulva | 504 | 463.5 ± 32.5 (425-504) | – |
| Cephalic region diam. | 12 | 11.5 ± 1.0 (10-12.5) | 12.5 ± 0.5 (12-13) |
| Cephalic region height | 5 | 5.0 ± 0.0 (5-5) | 4.7 ± 0.6 (4-5) |
| Odontostyle length | 33 | 33.0 ± 0.5 (32-33) | 35 ± 1 (34-36) |
| Odontophore length | 38 | 35.5 ± 2.5 (33-38) | 33.3 ± 3.0 (30-36) |
| Stylet total length | 71 | 68.5 ± 2.0 (66-71) | 68.5 ± 2.5 (66-71) |
| Guiding ring from ant. end | 2.8 | 18 ± 1 (18-20) | 18.0 ± 0.5 (18.0-18.5) |
| Neck length | 18 | 229 ± 2 (227-231) | 212 ± 8 (203-220) |
| Pharyngeal expansion length | 92 | 93 ± 1 (92-94) | 85.5 ± 5.5 (80-91) |
| Diam. at guiding ring level | 16 | 18.5 ± 0.0 (18.5-18.5) | 18.5 ± 0.0 (18.5-18.5) |
| -at neck base | 40 | 41.0 ± 3.5 (38-46) | 36.5 ± 2.5 (34-39) |
| -at mid-body | 43 | 44.0 ± 2.5 (41-47) | 37.0 ± 2.5 (35-40) |
| -at anus | 25 | 25.0 ± 0.5 (24-25) | 25 ± 1 (24-26) |
| Prerectum | 56 | 57.0 ± 7.5 (47-65) | 81 ± 20 (67-95) |
| Rectum | 22 | 23.5 ± 3.5 (19-27) | 38.0 ± 1.5 (37-39) |
| Tail length | 33 | 34.5 ± 2.5 (33-38) | 32.5 ± 4.0 (29-37) |
| Spicules length | – | – | 33.0 ± 1.5 (32-35) |
Figure 4:
Bayesian 50% majority rule consensus tree inferred from the 28S rDNA D2-D3 sequences of Longidorella (Saevadorella) caspica n. sp. under the GTR + G + I model. Bayesian posterior probabilities (BPP) and maximum likelihood bootstrap (ML BS) values > 0.50 are given for appropriate clades in the form: BPP/ML BS. The newly generated species is in bold font.