Table 1.
Morphometric data of Neothada major, N. hades and N. cancellata from Iran.
| Neothada major | N. hades | N. cancellata | |||||
|---|---|---|---|---|---|---|---|
| Present study | Maqbool and Shahina (1989) | Present study | Heyns and Van den Berg (1996) | Present study | |||
| n | 15 | cv | 15 | 10 | cv | 10 | 8 |
| L | 657±40.6 (600–728) | 6.1 | 640–800 | 586±50.3 (505–674) | 8.5 | 530–620 | 561±32.5 (501–596) |
| L' | 579±37.8 (521–645) | 6.5 | – | 515±48.1 (440–600) | 9.3 | – | 491±30.9 (431–527) |
| Head-Vulva | 461±27.2 (420–508) | 5.9 | – | 414±35.5 (354–467) | 8.5 | – | 396±24.4 (349–424) |
| R Head-Vulva | 142±11.7 (120–160) | 8.2 | 154–156 | 147±8.3 (127–157) | 5.6 | – | 146±9.9 (125–156) |
| R Head-Anus | 174±12.4 (149–194) | 7.1 | – | 175±9.2 (152–185) | 5.2 | – | 173± 11.1 (150–185) |
| Stylet | 10.9±0.3 (10.3–11.7) | 3.3 | 12–14.4 | 10.5±0.2 (10.0–10.8) | 2.1 | 9.0–10.5 | 10.8±0.2 (10.6–11.2) |
| a | 35.9±4.1 (29–44) | 11.4 | 34–39 | 30.3±2.0 (27.0–33.7) | 6.8 | 25–31 | 29.5±1.7 (27–32) |
| b | 5.7±0.4 (5.2–6.5) | 6.9 | 5.9–6.3 | 5.3±0.2 (4.8–5.7) | 5.4 | 5.3–6.4 | 5.2±0.2 (4.7–5.5) |
| c | 8.3±0.3 (7.5–8.7) | 3.7 | 9.0–10.2 | 8.3±0.4 (7.5–9.1) | 5.8 | 8.1–10.3 | 8.0±0.3 (7.5–8.6) |
| c' | 6.4±0.3 (5.6–7.1) | 6.1 | 5.5–6.0 | 6.0±0.3 (5.5–6.5) | 5.8 | 4.6–6.0 | 6.1±0.4 (5.5–6.6) |
| V | 70.2±0.7 (68.8–71.2) | 1.0 | 70–73 | 70.6±1.1 (68.5–72.7) | 1.6 | 70–74 | 70.9 ± 0.6 (70.0–71.8) |
| V' | 79.7±0.9 (78.0–81.1) | 1.1 | – | 80.3±1.3 (77.0–81.9) | 1.6 | – | 80.8±0.5 (80.4–82.4) |
| R | 202±12.5 (175–222) | 6.2 | 215–245 | 204±10.1 (179–216) | 4.9 | 149–160 | 189± 12.3 (175–191) |
| Excretory pore | 97.2±8.7 (75–110) | 9.0 | – | 94.7±5.8 (87–104) | 6.1 | – | 92.5±6.7 (86–100) |
| Pharynx | 114±7.6 (94–127) | 6.7 | 108–133 | 109.5±8.4 (95–119) | 7.7 | – | 107±9.3 (90–110) |
| R Pharynx | 41±3.0 (36–50) | 7.2 | 45–50 | 47±3.1 (43–52) | 6.6 | 30–38 | 45±2.5 (42–50) |
| Annulus width | 3.8±0.7 (3.2–5.6) | 20.0 | 3.2–4.0 | 2.9±0.2 (2.7–3.5) | 8.3 | 4.0–4.6 | 3.0±0.2 (2.6–3.6) |
| Body width | 18.4±1.7 (15.6–21.0) | 9.6 | – | 19.2±0.8 (17.8–21.0) | 4.5 | – | 18.8±0.6 (18–20) |
| Vulva body width | 17.5±1.5 (15–20) | 9.0 | – | 17.9±0.7 (16.5–19.0) | 4.0 | – | 17.5±0.7 (15.5–18.8) |
| Vulva-Anus | 117±11.8 (101–137) | 10.0 | – | 101.6±14.9 (86–138) | 14.7 | 95±7.1 (81–103) | |
| R Vulva-Anus | 32±3.8 (28–42) | 11.8 | 36–40 | 27±1.4 (25–29) | 5.4 | 21–26 | 27±1.5 (25–28) |
| Tail/Vulva-Anus | 0.6±0.1 (0.6–0.7) | 7.7 | – | 0.7±0.1 (0.5–0.7) | 10.1 | – | 0.7±0.1 (0.6–0.7) |
| Anal body width | 12.2±0.9 (10.8–14.0) | 7.3 | – | 11.5±0.5 (10.8–12.3) | 4.7 | – | 11.1±0.5 (10.5–12.0) |
| Tail length | 78.2±3.5 (70–83) | 4.5 | 67–80 | 70.4±2.7 (65–74) | 3.9 | 55–66 | 69±2.4 (65–71) |
Figure 1:
Iranian population of Neothada hades. Female (A-I): (A) Entire body; (B) Anterior end and pharyngeal region; (C) Lip region and stylet; (D) Stylet; (E) Reproductive system; (F) Amphidial aperture; (G) Cross section from mid-body; (H-I) Posterior end.
Figure 2:
Iranian population of Neothada hades. Female (A-K): (A) Entire body; (B-D) Lip region and stylet; (E) Amphidial aperture; (F) Cross section from mid-body; (G) Basal pharyngeal bulb and secretory-excretory pore; (H) Deirid and lateral field; (I-K) Posterior end. (Scale bars: A = 100 µm; B-K = 10 µm).
Figure 3:
Iranian population of Neothada major. Female (A-I): (A) Entire body; (B) Anterior end and pharynx; (C) Lip region; (D) Stylet; (E) Amphidial aperture; (F) Cross section from mid-body; (G-H) Posterior end; (I) Reproductive system.
Figure 4:
Iranian population of Neothada major. Female (A-N): (A) Entire body; (B) Anterior end and pharyngeal region; (C-F) Lip region and stylet; (G) Amphidial aperture; (H) Annules at mid-body; (I) Vulval region; (J-L) Posterior end; (M, N) Cross section from mid-body. (Scale bars: A = 100 µm; B-N = 10 µm).
Figure 5:
Iranian population of Neothada cancellata. Female (A-N): (A) Entire body; (B-F) Anterior end and stylet; (G) Vulval region; (H) Cross section from mid-body; (I-N) Posterior end.
Figure 6:
Bayesian Inference analysis tree under general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution model (GTR + I + G) from known and the newly sequenced Neothada hades and N. major based on sequences of the D2-D3 expansion fragments of the 28 S rDNA region. Bayesian posterior probabilities (50%) are given for each clade. Scale bar shows the number of substitutions per site.