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Morphological and molecular characterization of two species of Neothada Khan, 1973 (Nematoda: Tylenchidae) from Iran, with notes on N. cancellata

Open Access
|Feb 2021

Figures & Tables

Figure 1:

Iranian population of Neothada hades. Female (A-I): (A) Entire body; (B) Anterior end and pharyngeal region; (C) Lip region and stylet; (D) Stylet; (E) Reproductive system; (F) Amphidial aperture; (G) Cross section from mid-body; (H-I) Posterior end.
Iranian population of Neothada hades. Female (A-I): (A) Entire body; (B) Anterior end and pharyngeal region; (C) Lip region and stylet; (D) Stylet; (E) Reproductive system; (F) Amphidial aperture; (G) Cross section from mid-body; (H-I) Posterior end.

Figure 2:

Iranian population of Neothada hades. Female (A-K): (A) Entire body; (B-D) Lip region and stylet; (E) Amphidial aperture; (F) Cross section from mid-body; (G) Basal pharyngeal bulb and secretory-excretory pore; (H) Deirid and lateral field; (I-K) Posterior end. (Scale bars: A = 100 µm; B-K = 10 µm).
Iranian population of Neothada hades. Female (A-K): (A) Entire body; (B-D) Lip region and stylet; (E) Amphidial aperture; (F) Cross section from mid-body; (G) Basal pharyngeal bulb and secretory-excretory pore; (H) Deirid and lateral field; (I-K) Posterior end. (Scale bars: A = 100 µm; B-K = 10 µm).

Figure 3:

Iranian population of Neothada major. Female (A-I): (A) Entire body; (B) Anterior end and pharynx; (C) Lip region; (D) Stylet; (E) Amphidial aperture; (F) Cross section from mid-body; (G-H) Posterior end; (I) Reproductive system.
Iranian population of Neothada major. Female (A-I): (A) Entire body; (B) Anterior end and pharynx; (C) Lip region; (D) Stylet; (E) Amphidial aperture; (F) Cross section from mid-body; (G-H) Posterior end; (I) Reproductive system.

Figure 4:

Iranian population of Neothada major. Female (A-N): (A) Entire body; (B) Anterior end and pharyngeal region; (C-F) Lip region and stylet; (G) Amphidial aperture; (H) Annules at mid-body; (I) Vulval region; (J-L) Posterior end; (M, N) Cross section from mid-body. (Scale bars: A = 100 µm; B-N = 10 µm).
Iranian population of Neothada major. Female (A-N): (A) Entire body; (B) Anterior end and pharyngeal region; (C-F) Lip region and stylet; (G) Amphidial aperture; (H) Annules at mid-body; (I) Vulval region; (J-L) Posterior end; (M, N) Cross section from mid-body. (Scale bars: A = 100 µm; B-N = 10 µm).

Figure 5:

Iranian population of Neothada cancellata. Female (A-N): (A) Entire body; (B-F) Anterior end and stylet; (G) Vulval region; (H) Cross section from mid-body; (I-N) Posterior end.
Iranian population of Neothada cancellata. Female (A-N): (A) Entire body; (B-F) Anterior end and stylet; (G) Vulval region; (H) Cross section from mid-body; (I-N) Posterior end.

Figure 6:

Bayesian Inference analysis tree under general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution model (GTR + I + G) from known and the newly sequenced Neothada hades and N. major based on sequences of the D2-D3 expansion fragments of the 28 S rDNA region. Bayesian posterior probabilities (50%) are given for each clade. Scale bar shows the number of substitutions per site.
Bayesian Inference analysis tree under general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution model (GTR + I + G) from known and the newly sequenced Neothada hades and N. major based on sequences of the D2-D3 expansion fragments of the 28 S rDNA region. Bayesian posterior probabilities (50%) are given for each clade. Scale bar shows the number of substitutions per site.

Morphometric data of Neothada major, N_ hades and N_ cancellata from Iran_

Neothada majorN. hadesN. cancellata
Present studyMaqbool and Shahina (1989)Present studyHeyns and Van den Berg (1996)Present study
n15cv1510cv108
L657±40.6 (600–728)6.1640–800586±50.3 (505–674)8.5530–620561±32.5 (501–596)
L'579±37.8 (521–645)6.5515±48.1 (440–600)9.3491±30.9 (431–527)
Head-Vulva461±27.2 (420–508)5.9414±35.5 (354–467)8.5396±24.4 (349–424)
R Head-Vulva142±11.7 (120–160)8.2154–156147±8.3 (127–157)5.6146±9.9 (125–156)
R Head-Anus174±12.4 (149–194)7.1175±9.2 (152–185)5.2173± 11.1 (150–185)
Stylet10.9±0.3 (10.3–11.7)3.312–14.410.5±0.2 (10.0–10.8)2.19.0–10.510.8±0.2 (10.6–11.2)
a35.9±4.1 (29–44)11.434–3930.3±2.0 (27.0–33.7)6.825–3129.5±1.7 (27–32)
b5.7±0.4 (5.2–6.5)6.95.9–6.35.3±0.2 (4.8–5.7)5.45.3–6.45.2±0.2 (4.7–5.5)
c8.3±0.3 (7.5–8.7)3.79.0–10.28.3±0.4 (7.5–9.1)5.88.1–10.38.0±0.3 (7.5–8.6)
c'6.4±0.3 (5.6–7.1)6.15.5–6.06.0±0.3 (5.5–6.5)5.84.6–6.06.1±0.4 (5.5–6.6)
V70.2±0.7 (68.8–71.2)1.070–7370.6±1.1 (68.5–72.7)1.670–7470.9 ± 0.6 (70.0–71.8)
V'79.7±0.9 (78.0–81.1)1.180.3±1.3 (77.0–81.9)1.680.8±0.5 (80.4–82.4)
R202±12.5 (175–222)6.2215–245204±10.1 (179–216)4.9149–160189± 12.3 (175–191)
Excretory pore97.2±8.7 (75–110)9.094.7±5.8 (87–104)6.192.5±6.7 (86–100)
Pharynx114±7.6 (94–127)6.7108–133109.5±8.4 (95–119)7.7107±9.3 (90–110)
R Pharynx41±3.0 (36–50)7.245–5047±3.1 (43–52)6.630–3845±2.5 (42–50)
Annulus width3.8±0.7 (3.2–5.6)20.03.2–4.02.9±0.2 (2.7–3.5)8.34.0–4.63.0±0.2 (2.6–3.6)
Body width18.4±1.7 (15.6–21.0)9.619.2±0.8 (17.8–21.0)4.518.8±0.6 (18–20)
Vulva body width17.5±1.5 (15–20)9.017.9±0.7 (16.5–19.0)4.017.5±0.7 (15.5–18.8)
Vulva-Anus117±11.8 (101–137)10.0101.6±14.9 (86–138)14.7 95±7.1 (81–103)
R Vulva-Anus32±3.8 (28–42)11.836–4027±1.4 (25–29)5.421–2627±1.5 (25–28)
Tail/Vulva-Anus0.6±0.1 (0.6–0.7)7.70.7±0.1 (0.5–0.7)10.10.7±0.1 (0.6–0.7)
Anal body width12.2±0.9 (10.8–14.0)7.311.5±0.5 (10.8–12.3)4.711.1±0.5 (10.5–12.0)
Tail length78.2±3.5 (70–83)4.567–8070.4±2.7 (65–74)3.955–6669±2.4 (65–71)
DOI: https://doi.org/10.21307/jofnem-2020-131 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 10
Published on: Feb 2, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2021 Manouchehr Hosseinvand, Ali Eskandari, Reza Ghaderi, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.