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Further observations on Meloidogyne enterolobii (Nematoda: Meloidogynidae) infecting guava (Psidium guajava) in India

Open Access
|Dec 2020

Figures & Tables

Figure 1:

Symptoms induced by Meloidogyne enterolobii Yang and Eisenback, 1983 in guava. A: infested guava plant; B: infected plant root showing heavy galling.
Symptoms induced by Meloidogyne enterolobii Yang and Eisenback, 1983 in guava. A: infested guava plant; B: infected plant root showing heavy galling.

Figure 2:

Photomicrographs of perineal patterns of Meloidogyne enterolobii Yang and Eisenback, 1983. (scale bar = 20 µm).
Photomicrographs of perineal patterns of Meloidogyne enterolobii Yang and Eisenback, 1983. (scale bar = 20 µm).

Figure 3:

Photomicrographs of female, male, and second-stage juveniles (J2s). A: Anterior end of female; B, C: Anterior ends of male (lateral, dorsal); D: Posterior end of male; E, F: Anterior ends of J2; G, H: Posterior ends of J2 (arrow showing the constriction). (scale bar = 20 µm).
Photomicrographs of female, male, and second-stage juveniles (J2s). A: Anterior end of female; B, C: Anterior ends of male (lateral, dorsal); D: Posterior end of male; E, F: Anterior ends of J2; G, H: Posterior ends of J2 (arrow showing the constriction). (scale bar = 20 µm).

Figure 4:

Biochemical and molecular characterization of Meloidogyne enterolobii Yang and Eisenback, 1983 population isolated from Tamil Nadu, India. A: Esterase enzyme phenotypic pattern (J3: M. javanica (Treub, 1885) Chitwood, 1949; M2: M. enterolobii Yang and Eisenback, 1983); B: PCR amplification with SCAR marker (M: DNA weight marker; lanes 1 and 2: M. incognita (Kofoid and White, 1919) Chitwood, 1949 control; lane 3: M. javanica (Treub, 1885) Chitwood, 1949 control; lanes 4-6: M. enterolobii Yang and Eisenback, 1983 populations).
Biochemical and molecular characterization of Meloidogyne enterolobii Yang and Eisenback, 1983 population isolated from Tamil Nadu, India. A: Esterase enzyme phenotypic pattern (J3: M. javanica (Treub, 1885) Chitwood, 1949; M2: M. enterolobii Yang and Eisenback, 1983); B: PCR amplification with SCAR marker (M: DNA weight marker; lanes 1 and 2: M. incognita (Kofoid and White, 1919) Chitwood, 1949 control; lane 3: M. javanica (Treub, 1885) Chitwood, 1949 control; lanes 4-6: M. enterolobii Yang and Eisenback, 1983 populations).

Morphometrics of Meloidogyne enterolobii Yang and Eisenback, 1983 males from Tamil Nadu, India; compared with earlier descriptions of M_ enterolobii_

CharactersPresent population (n = 20) M. enterolobii Yang and Eisenback, 1983 M. enterolobii (Karssen et al., 2012)
Body length1089.1±236.6 (800-1460)1599.8±159.91 (1348.6-1913.3)1230±316 (865-1667)
Body width27.0±3.7 (20.9-33.2)42.3±3.5 (37.0-48.3)32.0±6.0 (23.7-39.2)
Tail length12.5±2.24 (8.6-20.2)11.9±1.2 (10.2-13.4)
Lip height5.9±0.5 (4.7-6.6)
Lip width9.9±0.7 (8.5-11.4)
Stylet length21.2±1.3 (19.0-22.8)23.4±0.96 (21.2-25.5)21.5±1.7 (19.2-23.4)
Stylet cone9.7±0.8 (8.5-11.4)
Stylet shaft8.3±0.6 (7.1-9.5)
Stylet knob length3.2±0.5 (2.3-3.8)3.3±0.33 (2.6-3.9)2.5±0.3 (2.1-3.2)
Stylet knob width4.3±0.4 (3.8-7.6)5.4±0.34 (4.5-5.8)4.5±0.6 (3.5-5.0)
DEGO5.3±1.0 (3.8-7.6)4.7±0.4 (3.7-5.3)4.7±0.6 (3.7-5.8)
Head-metacorpus86.2±7.9 (74.1-101.6)
Metacarpus length20.1±2.5 (17.1-25.6)
Metacarpus width9.5±0.9 (8.5-11.4)
Metacarpus-gland end111.3±12.7 (93.1-133.0)
Head-SE pore130.4±21.7 (86.4-153.9)178.2±11.2 (159.7-206.2)155.8±22.3 (129.9-199.7)
Spicule length25.2±4.3 (17.1-31.3)30.4±1.2 (27.3-32.1)28.0±1.1 (26.2-29.4)
Gubernaculum length7.5±0.8 (5.7-8.5)6.2±1.0 (4.8-8.0)6.5±0.8 (6.1-8.0)
Testis length300.9±84.4 (190.0-422.7)
a 40.2±6.0 (32.1-51.2)37.9± 3.2 (34.1-45.5)38.1±4.0 (30.0-43.4)
b″ 12.58±2.21 (9.9-16.1)
Body length/head end to posterior end of metacorpus15.8±1.32 (13.8-18.4)-
c 131.6±24.1 (72.0-173.4)103.2±23.7 (71.4-135.9)

Morphometrics of Meloidogyne enterolobii Yang and Eisenback, 1983 second-stage juveniles (J2s) from Tamil Nadu, India; compared with earlier descriptions of M_ enterolobii_

CharacterPresent population (n = 20) M. enterolobii Yang and Eisenback, 1983 M. enterolobii (Karssen et al., 2012)
Body length413.5±26.7 (375-460)436.6±16.6 (405.0-472.9)408±18 (380-442)
Body width13.9±1.5 (12.3-17.1)15.3±0.9 (13.9-17.8)14.8±2.1 (11.0-18.0)
Body width at anus10.2±1.3 (8.5-13.3)9.8±0.9 (8.0-11.0)
a 30.0±3.2 (24.1-35.2)28.6±1.9 (24.0-32.5)28.0±3.7 (23.3-34.6)
Stylet length12.1±0.4 (11.4-12.3)11.7±0.5 (10.8-13.0)11.3±0.7 (10.5-13.0)
Stylet knob height1.6±0.1 (1.9-1.8)1.8±0.3 (1.5-2.0)
Stylet knob width2.9±0.2 (2.4-3.4)3.0±0.4 (2.5-4.0)
Stylet base to head end15.0±0.7 (14.0-16.0)
DGO2.8±0.3 (2.3-3.8)3.4±0.3 (2.8-4.3)3.8±0.3 (3.0-4.5)
Length median bulb12.3±0.7 (11.4-13.3)
Width median bulb7.5±0.9 (6.6-9.5)
Head-metacorpus56.1±2.3 (51.3-59.8)
b″ 7.3±0.5 (6.3-8.4)
Head-SE pore78.9±4.3 (73.1-92.1)91.7±3.3 (84.0-98.6)80.8±4.4 (70.0-88.0)
Tail length50.1±7.0 (42.7-75.1)56.4±4.5 (41.5-63.4)52.1±3.4 (45.0-57.0)
c 8.3±1.0 (6.1-10.6)7.8±0.7 (6.8-10.1)7.9±0.6 (7.0-9.0)
Body length/head end to posterior end of metacorpus6.5±0.1 (6.2-6.9)
Hyaline tail terminus length13.5±1.31 (11.4-16.1)
c′ 4.9±0.5 (4.3-5.7)
Rectum inflatedall

Morphometrics of Meloidogyne enterolobii Yang and Eisenback, 1983 females from Tamil Nadu, India; compared with earlier descriptions of M_ enterolobii_

CharactersPresent population (n = 20) M. enterolobii Yang and Eisenback, 1983 M. enterolobii (Karssen et al., 2012)
Body length617.0±59.7 (520-710)735.0±92.8 (541.3-926.3)693
Body width465.5±54.8 (380-580)606.8±120.5 (375.7-809.7)462
Neck length218.4±74.1 (114.3-466.8)262
Stylet length13.4±0.4 (13.1-14.2)15.1±1.35 (13.2-18.0)14.7
Stylet knob height2.4±0.26 (1.9-3.1)2.3
Stylet knob width4.9±0.39 (4.1-5.6)4.5
Stylet cone6.3±1.2 (5.6-9.5)
DEGO4.3±0.6 (3.7-5.7)4.9±0.78 (3.7-6.2)4.8
Length median bulb36.1±3.3 (31.0-42.7)
Width median bulb26.6±4.6 (20.6-35.7)
Excretory pore to head end64.9±3.4 (57.7-69.6)62.9±10.5 (42.3-80.6)64.0
Length vulva-slit24.8±1.4 (22.8-27.5)28.7±2.0 (25.3–32.4)28.0±1.0 (25.9-29.1)
Inter-phasmid distance24.6±3.4 (19.0-29.4)30.7±4.8 (22.2-42.0)33.5±7.6 (22.4-41.9)
Vulva-anus20.2±0.9 (19.0-21.8)22.2±1.8 (19.7-26.6)23.4±1.6 (21.1-26.2)
Vulval slit-tail terminus32.9±2.1 (29.4-37.0)
a1.3±0.1 (1.1-1.5)1.2±0.2 (0.9-1.9)
Body length/head end to posterior end of metacorpus7.1±0.9 (5.1-9.3)
DOI: https://doi.org/10.21307/jofnem-2020-120 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 9
Published on: Dec 14, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:

© 2020 Tushar Manohar Ghule, Victor Phani, Vishal Singh Somvanshi, Maya Patil, Somnath Bhattacharyya, Matiyar Rahaman Khan, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.