Table 1.
Morphometrics of Meloidogyne enterolobii Yang and Eisenback, 1983 females from Tamil Nadu, India; compared with earlier descriptions of M. enterolobii.
| Characters | Present population (n = 20) | M. enterolobii Yang and Eisenback, 1983 | M. enterolobii (Karssen et al., 2012) |
|---|---|---|---|
| Body length | 617.0±59.7 (520-710) | 735.0±92.8 (541.3-926.3) | 693 |
| Body width | 465.5±54.8 (380-580) | 606.8±120.5 (375.7-809.7) | 462 |
| Neck length | – | 218.4±74.1 (114.3-466.8) | 262 |
| Stylet length | 13.4±0.4 (13.1-14.2) | 15.1±1.35 (13.2-18.0) | 14.7 |
| Stylet knob height | – | 2.4±0.26 (1.9-3.1) | 2.3 |
| Stylet knob width | – | 4.9±0.39 (4.1-5.6) | 4.5 |
| Stylet cone | 6.3±1.2 (5.6-9.5) | – | – |
| DEGO | 4.3±0.6 (3.7-5.7) | 4.9±0.78 (3.7-6.2) | 4.8 |
| Length median bulb | 36.1±3.3 (31.0-42.7) | – | – |
| Width median bulb | 26.6±4.6 (20.6-35.7) | – | – |
| Excretory pore to head end | 64.9±3.4 (57.7-69.6) | 62.9±10.5 (42.3-80.6) | 64.0 |
| Length vulva-slit | 24.8±1.4 (22.8-27.5) | 28.7±2.0 (25.3–32.4) | 28.0±1.0 (25.9-29.1) |
| Inter-phasmid distance | 24.6±3.4 (19.0-29.4) | 30.7±4.8 (22.2-42.0) | 33.5±7.6 (22.4-41.9) |
| Vulva-anus | 20.2±0.9 (19.0-21.8) | 22.2±1.8 (19.7-26.6) | 23.4±1.6 (21.1-26.2) |
| Vulval slit-tail terminus | 32.9±2.1 (29.4-37.0) | – | – |
| a | 1.3±0.1 (1.1-1.5) | 1.2±0.2 (0.9-1.9) | – |
| Body length/head end to posterior end of metacorpus | – | 7.1±0.9 (5.1-9.3) | – |
Table 2.
Morphometrics of Meloidogyne enterolobii Yang and Eisenback, 1983 males from Tamil Nadu, India; compared with earlier descriptions of M. enterolobii.
| Characters | Present population (n = 20) | M. enterolobii Yang and Eisenback, 1983 | M. enterolobii (Karssen et al., 2012) |
|---|---|---|---|
| Body length | 1089.1±236.6 (800-1460) | 1599.8±159.91 (1348.6-1913.3) | 1230±316 (865-1667) |
| Body width | 27.0±3.7 (20.9-33.2) | 42.3±3.5 (37.0-48.3) | 32.0±6.0 (23.7-39.2) |
| Tail length | – | 12.5±2.24 (8.6-20.2) | 11.9±1.2 (10.2-13.4) |
| Lip height | 5.9±0.5 (4.7-6.6) | – | – |
| Lip width | 9.9±0.7 (8.5-11.4) | – | – |
| Stylet length | 21.2±1.3 (19.0-22.8) | 23.4±0.96 (21.2-25.5) | 21.5±1.7 (19.2-23.4) |
| Stylet cone | 9.7±0.8 (8.5-11.4) | – | – |
| Stylet shaft | 8.3±0.6 (7.1-9.5) | – | – |
| Stylet knob length | 3.2±0.5 (2.3-3.8) | 3.3±0.33 (2.6-3.9) | 2.5±0.3 (2.1-3.2) |
| Stylet knob width | 4.3±0.4 (3.8-7.6) | 5.4±0.34 (4.5-5.8) | 4.5±0.6 (3.5-5.0) |
| DEGO | 5.3±1.0 (3.8-7.6) | 4.7±0.4 (3.7-5.3) | 4.7±0.6 (3.7-5.8) |
| Head-metacorpus | 86.2±7.9 (74.1-101.6) | – | – |
| Metacarpus length | 20.1±2.5 (17.1-25.6) | – | – |
| Metacarpus width | 9.5±0.9 (8.5-11.4) | – | – |
| Metacarpus-gland end | 111.3±12.7 (93.1-133.0) | – | – |
| Head-SE pore | 130.4±21.7 (86.4-153.9) | 178.2±11.2 (159.7-206.2) | 155.8±22.3 (129.9-199.7) |
| Spicule length | 25.2±4.3 (17.1-31.3) | 30.4±1.2 (27.3-32.1) | 28.0±1.1 (26.2-29.4) |
| Gubernaculum length | 7.5±0.8 (5.7-8.5) | 6.2±1.0 (4.8-8.0) | 6.5±0.8 (6.1-8.0) |
| Testis length | 300.9±84.4 (190.0-422.7) | – | – |
| a | 40.2±6.0 (32.1-51.2) | 37.9± 3.2 (34.1-45.5) | 38.1±4.0 (30.0-43.4) |
| b″ | 12.58±2.21 (9.9-16.1) | – | – |
| Body length/head end to posterior end of metacorpus | – | 15.8±1.32 (13.8-18.4) | - |
| c | – | 131.6±24.1 (72.0-173.4) | 103.2±23.7 (71.4-135.9) |
Table 3.
Morphometrics of Meloidogyne enterolobii Yang and Eisenback, 1983 second-stage juveniles (J2s) from Tamil Nadu, India; compared with earlier descriptions of M. enterolobii.
| Character | Present population (n = 20) | M. enterolobii Yang and Eisenback, 1983 | M. enterolobii (Karssen et al., 2012) |
|---|---|---|---|
| Body length | 413.5±26.7 (375-460) | 436.6±16.6 (405.0-472.9) | 408±18 (380-442) |
| Body width | 13.9±1.5 (12.3-17.1) | 15.3±0.9 (13.9-17.8) | 14.8±2.1 (11.0-18.0) |
| Body width at anus | 10.2±1.3 (8.5-13.3) | – | 9.8±0.9 (8.0-11.0) |
| a | 30.0±3.2 (24.1-35.2) | 28.6±1.9 (24.0-32.5) | 28.0±3.7 (23.3-34.6) |
| Stylet length | 12.1±0.4 (11.4-12.3) | 11.7±0.5 (10.8-13.0) | 11.3±0.7 (10.5-13.0) |
| Stylet knob height | – | 1.6±0.1 (1.9-1.8) | 1.8±0.3 (1.5-2.0) |
| Stylet knob width | – | 2.9±0.2 (2.4-3.4) | 3.0±0.4 (2.5-4.0) |
| Stylet base to head end | – | – | 15.0±0.7 (14.0-16.0) |
| DGO | 2.8±0.3 (2.3-3.8) | 3.4±0.3 (2.8-4.3) | 3.8±0.3 (3.0-4.5) |
| Length median bulb | 12.3±0.7 (11.4-13.3) | – | – |
| Width median bulb | 7.5±0.9 (6.6-9.5) | – | – |
| Head-metacorpus | 56.1±2.3 (51.3-59.8) | – | – |
| b″ | 7.3±0.5 (6.3-8.4) | – | – |
| Head-SE pore | 78.9±4.3 (73.1-92.1) | 91.7±3.3 (84.0-98.6) | 80.8±4.4 (70.0-88.0) |
| Tail length | 50.1±7.0 (42.7-75.1) | 56.4±4.5 (41.5-63.4) | 52.1±3.4 (45.0-57.0) |
| c | 8.3±1.0 (6.1-10.6) | 7.8±0.7 (6.8-10.1) | 7.9±0.6 (7.0-9.0) |
| Body length/head end to posterior end of metacorpus | – | 6.5±0.1 (6.2-6.9) | – |
| Hyaline tail terminus length | 13.5±1.31 (11.4-16.1) | – | – |
| c′ | 4.9±0.5 (4.3-5.7) | – | – |
| Rectum inflated | all | – | – |
Figure 1:
Symptoms induced by Meloidogyne enterolobii Yang and Eisenback, 1983 in guava. A: infested guava plant; B: infected plant root showing heavy galling.
Figure 2:
Photomicrographs of perineal patterns of Meloidogyne enterolobii Yang and Eisenback, 1983. (scale bar = 20 µm).
Figure 3:
Photomicrographs of female, male, and second-stage juveniles (J2s). A: Anterior end of female; B, C: Anterior ends of male (lateral, dorsal); D: Posterior end of male; E, F: Anterior ends of J2; G, H: Posterior ends of J2 (arrow showing the constriction). (scale bar = 20 µm).
Figure 4:
Biochemical and molecular characterization of Meloidogyne enterolobii Yang and Eisenback, 1983 population isolated from Tamil Nadu, India. A: Esterase enzyme phenotypic pattern (J3: M. javanica (Treub, 1885) Chitwood, 1949; M2: M. enterolobii Yang and Eisenback, 1983); B: PCR amplification with SCAR marker (M: DNA weight marker; lanes 1 and 2: M. incognita (Kofoid and White, 1919) Chitwood, 1949 control; lane 3: M. javanica (Treub, 1885) Chitwood, 1949 control; lanes 4-6: M. enterolobii Yang and Eisenback, 1983 populations).