Morphological characters of 3rd stage infective juveniles for three entomopathogenic nematode isolates from Montana, USA_
| Character | Steinernema feltiae 1 | Steinernema feltiae 2 | ANOVA results | Heterorhabditis bacteriophora |
|---|---|---|---|---|
| L | 820.25±11.16 (711-900)a | 807.85±9.60 (743-906)a | t=0.82, df=37, P=0.11 | 602.25±9.48 (513-654) |
| D | 28.48±0.73 (22-34.4)a | 29.93±0.57 (26-35)a | t=−1.63, df=36, P=0.11 | 24.74±0.64 (18-31) |
| EP | 64.45±1.94 (48-80)a | 55.25±1.72 (43-67)b | t=3.45, df=37, P=0.001 | 77.35±2.24 (55-95) |
| NR | 72±2.83 (42-91)a | 59.65±2.01 (48-81)b | t=3.47, df=34, P=0.001 | 78.2±1.32 (68-90) |
| ES | 90.2±2.97 (61-114)a | 87.7±2.02 (48-81)a | t=0.68, df=33, P=0.50 | 106.5±3.10 (93-140) |
| T | 79.34±1.49 (61-90)a | 80.85±1.09 (74-89)a | t=−0.82, df=35, P=0.42 | 91.26±4.52 (56-140.3) |
| A | 29.15±0.81 (24.58-39.68)a | 27.19±0.6 (23.23-32.78)a | t=1.90, df=36, P=0.07 | 24.34±0.60 (14.93-28.5) |
| B | 9.31±0.36 (7.45-13.15)a | 9.31±0.25 (7.40-11.13)a | t=0.0, df=34, P=1.00 | 5.65±0.18 (3.063-5.52) |
| C | 10.40±0.21 (8.67-13.14)a | 10.02±0.16 (8.78-11.19)a | t=1.63, df=37, P=0.11 | 6.60±0.29 (2.10-9.16) |
| D% | 72.42±2.36 (55.24-87.91)a | 63.62±2.37 (39.81-83.33)a | t=−1.63, df=36, P=0.11 | 72.63±2.61 (72.29-59.14) |
| E% | 81.60±2.63 (63.16-108.11)a | 68.42±2.11 (51.81-86.84)b | t=3.82, df=36, P=0.0005 | 84.75±5.04 (49.52-98.21) |
Morphological characteristics of males of three entomopathogenic nematode isolates from Montana, USA_
| Character | Steinernema feltiae 1 (1st gen.) | Steinernema feltiae 2 (1st gen.) | t-test results | Heterorhabditis bacteriophora (2nd gen.) |
|---|---|---|---|---|
| L | 1570.35±36.84 (1259-1813)a | 1304.8±43.3 (913-1570)b | t=4.55, df=37, P<0.0001 | 802.1±20.16 (610-920) |
| D | 96.93±3.01 (76-130)a | 96.05±3.14 (65-119)a | t=0.20, df=38, P=0.85 | 62.3±1.99 (50-78) |
| EP | 90.06±2.25 (73-115)a | 93±1.55 (84-109)a | t=−1.05, df=34, P=0.30 | 77.9±1.17 (70-90) |
| NR | 98.35±1.63 (88-113)a | 100±1.18 (88-110)a | t=−0.80, df=35, P=0.43 | 80.45±1.32 (70-91) |
| ES | 145.55±2.80 (121-167)b | 164.55±1.72 (154-180)a | t=−5.63, df=32, P<0.0001 | 110.25±1.97 (97-129) |
| T | 41.65±0.75 (36-48)b | 52.75±2.62 (34-72) a | t=−3.96, df=22, P=0.0006 | 61.58±3.59 (41-110) |
| ABD | 44.72±1.07 (35-51)b | 62.85±3.00 (45-91)a | t=−5.56, df=24, P<0.0001 | 34.51±0.67 (30-39.4) |
| SP | 80.35±1.41 (67-92)a | 71.5±2.21 (58-98)b | t=4.49, df=36, P<0.0001 | 41±1.26 (32-51) |
| GU | 49.95±1.11 (41-59) a | 45.85±0.86 (40-53) b | t=2.85, df=36, P=0.007 | 22.95±0.74 (14-28) |
| D% | 62.09±1.47 (49.08-72.34)a | 56.56±0.86 (51.14-65.19)a | t=0.20, df=38, P=0.85 | 70.90±1.19 (63.2-83.50) |
| SW% | 181.27±4.51 (145.65-235.90)a | 117.34±6.81 (63.74-168)a | t=7.63, df=33, P<0.0001 | 119.83±4.69 (91.37-164.52) |
| GS% | 62.74±2.13 (49.40-85.51)a | 66.35±2.16 (51.19-91.38)a | t=−1.16, df=38, P=0.25 | 56.85±2.61 (41.18-87.5) |
Different agricultural field sites surveyed in Montana, USA, for native entomopathogenic nematodes during 2018_
| Sampling site | Farmer | Latitude | Longitude | Elevation (m) | Vegetation | Soil texture | Soil pH | Organic Matter (%) | Presence/Absence of EPNs | Re-culture* |
|---|---|---|---|---|---|---|---|---|---|---|
| Pendroy | John Stoltz | N48.04130° | W112.16945° | 4220 | Wheat/Canola | Clay loam | 8.2 | 3.9 | +(3) | No |
| Pendroy | Kevin Johnson | N48.04206° | W112.20099° | 4328 | Wheat | Sandy clay loam | 7.9 | 4.4 | +(1) | No |
| Choteau | Joe Miller | N47.56785° | W112.13926° | 3906 | Fallow | Clay | 7.8 | 3.2 | − | − |
| Choteau | Mike Leys | N47.90238° | W112.3802° | − | Wheat | Sandy clay loam | 8 | 3.3 | +(2) | Yes (1 Steinernematid and 1 Heterorhabditid) |
| Valier | John Majerus | N48.18454° | W111.55524° | − | Wheat | Sandy clay loam | 8.1 | 3.4 | − | |
| Conrad | Mark Grub | N48.6334° | W111.53175° | − | Peas | Sandy clay loam | 7 | 2.9 | − | − |
| Conrad | Mark Orcutt | N48.16259° | W111.52896° | 3498 | Fallow | Clay loam | 8.1 | 2.3 | − | − |
| Conrad | Garett Grub | N47.56786° | W112.13930° | − | Wheat/Vegetables | Sandy clay loam | 7.8 | 2.4 | +(2) | No |
| Kalispell | Ron De Yong | N48.1945° | W114.2341° | − | Fallow/Winter wheat | Loam | 8.1 | 3.4 | +(1) | Yes (1 Steinernematid) |
| Valier | Jeremy Curry | N48.3032° | W112.4055° | 3749 | Fallow/Wheat | Sandy clay loam | 8.2 | 2.1 | +(2) | No |
| Conrad | Zane Drishinski | N48.77764° | W111.8948° | 3508 | Alfalfa | Clay loam | 6.8 | 2.8 | − | − |
| Conrad | WTARC | N48.3076° | W111.9255° | − | Fallow/Wheat | Clay loam | 7.8 | 2.9 | − | − |
| Conrad | Garman | N48.27808° | W111.92616° | 1116.05 | Alfalfa | Clay loam | 8.1 | 3.7 | − | − |
| Choteau (Knees) | A Killion | N48.00729° | W111.36596° | 1081.26 | Chickpea | Clay loam | 8.2 | 2.5 | − | − |
| Knees | A Killion | N48.00729° | W111.36596° | 1081.26 | Flax | Clay loam | 7.5 | 3.5 | +(1) | No |
| Brady | Winterhood | N47.98468° | W111.84313° | 1112.80 | Fallow/Winter wheat | Sandy clay loam | 7.3 | 1.9 | +(1) | No |
| Collins | Chickenhead | N47.95588° | W111.90971° | 1122.68 | Chickpea | Clay | 7.8 | 2.3 | +(1) | No |
| Choteau | N47.91965° | W112.04917° | 1177.36 | Hemp | Clay | 8.2 | 2.8 | − | − | |
| Choteau | Nesbitt | N47.79007° | W111.16589° | 1224.32 | Durum Wheat | Clay loam | 6.6 | 4.4 | − | − |
| Dutton | Tims | N47.83529° | W111.53990° | 1144.20 | Lentils | Clay loam | 7.9 | 3.4 | − | − |
| Shelby west | John Wigand | N48.56643° | W111.98537° | 1103.57 | Peas | Sandy clay loam | 6.9 | 3.9 | − | − |
| Shelby west | John Wigand | N48.57870° | W111.99317° | 1087.12 | Triticale | Sandy loam | 7.6 | 2.6 | − | − |
| Shelby West | John Wigand | N48.54374° | W111.99096° | 1071.82 | Fallow | Clay loam | 7.6 | 2.3 | − | − |
| Potter Rd Shelby | John Wigand | N48.65339° | W111.96044° | 1057.40 | Fallow | Clay loam | 7.9 | 2.7 | − | − |
| Sunburst | Koeye Fauque | N48.82692° | W111.85641° | 1093.77 | Chickpea | Sandy clay loam | 8 | 2.9 | +(1) | No |
| Sunburst | Koeye Fauque | N48.82701° | W111.81002° | 1102.46 | Spring wheat | Clay loam | 8.2 | 2.3 | − | − |
| Tiber | Duncan | N48.19011° | W111.25206° | 993.03 | Fallow | Clay loam | 8.2 | 2.6 | − | − |
| Tiber | Paul Kolstad | N48.17184° | W111.17372° | 960.91 | Fallow | Clay loam | 8.1 | 2.3 | − | − |
| Tiber | Wicks | N48.23188° | W111.14511° | 1029.37 | Lentil/Fallow | Clay loam | 7.7 | 2.5 | − | − |
| Tiber | Broadhurst | N48.27280° | W111.14571° | 930.70 | Fallow | Clay loam | 8.1 | 2.6 | − | − |