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Number of plant-parasitic nematodes of six different genera counted in nematode extract from 100cc of soil from 12 sugarcane plantation sites at Tanganyika Planting Company Limited in Kilimanjaro region of Tanzania in July, 2017_
| Collection sites | GPS coordinates | Rotylenchulus | Pratylenchus | Tylenchorhynchus | Scutellonema | Hemicycliophora | Helicotylenchus |
|---|---|---|---|---|---|---|---|
| N50-North | 3° 25´ 1.20˝ S, 37° 18´ 49.68˝ E | 33 | 18 | 0 | 5 | 0 | 0 |
| N54-North | 3° 25´ 20.32˝ S, 37° 18´ 49.68˝ E | 117 | 6 | 3 | 0 | 0 | 1 |
| N84-North | 3° 23´ 58.34˝ S, 37° 20´ 1.43˝ E | 14 | 13 | 0 | 0 | 0 | 0 |
| D8-East | 3° 28´ 1.38˝ S, 37° 20´ 15.04˝ E | 7 | 11 | 0 | 0 | 0 | 0 |
| D30-East | 3° 30´ 11.12˝ S, 37° 20´ 56.86˝ E | 21 | 11 | 0 | 0 | 0 | 0 |
| C6-East | 3° 28´ 2.25˝ S, 37° 19´ 33.68˝ E | 12 | 2 | 0 | 0 | 5 | 1 |
| D20-East | 3° 29´ 1.62˝ S, 37° 20´ 42.02˝ E | 12 | 20 | 1 | 1 | 7 | 1 |
| E11-East | 3° 28´ 58.72˝ S, 37° 21´ 0.07˝ E | 22 | 84 | 1 | 0 | 0 | 0 |
| F13-South | 3° 33´ 44.52˝ S, 37° 18´ 51.03˝ E | 1037 | 11 | 5 | 0 | 0 | 0 |
| F10-South | 3° 31´ 9.67˝ S, 37° 20´ 15.71´ E | 170 | 0 | 16 | 0 | 1 | 0 |
| 11E-South | 3° 28´ 28.45˝ S, 37° 20´ 44.26˝ E | 7 | 15 | 1 | 0 | 0 | 0 |
| R7S-South | 3° 29´ 48.49˝ S, 37° 18´ 10.96˝ E | 47 | 10 | 0 | 0 | 0 | 0 |
Morphometrics of immature females and males of Rotylenchulus parvus collected from F13-South of Tanganyika Planting Company Limited in Kilimanjaro region of Tanzania_
| Character | Immature females | Males |
|---|---|---|
| n | 28 | 5 |
| L | 327 ± 29 (271-352) | 393 ± 38 (342-426) |
| a | 24.9 ± 1.6 (21.9-26.8) | 31.1 ± 1.6 (29.4-33.2) |
| b | 3.2 ± 0.5 (2.6-3.9) | 3.7 ± 0.2 (3.5-3.9) |
| c | 15.3 ± 0.4 (12.3-17.5) | 17.0 ± 0.9 (16.0-18.1) |
| DGO | 17.3 ± 1.1 (16.6-18.0) | – |
| V | 62% (60%-66%) | – |
| Stylet length | 14.5 ± 0.4 (13.1-15.4) | 11.9 ± 0.3 (11.7-12.3) |
| Metenchium length | 6.4 ± 0.3 (6.0-6.5) | 5.2 ± 0.1 (5.2-5.3) |
| Telenchium length | 8.1 ± 0.2 (6.3-8.7) | 6.6 ± 0.2 (6.4-6.9) |
| Stylet knob width | 2.7 ± 0.3 (2.5-2.9) | 1.5 ± 0.2 (1.4-1.6) |
| Stylet knob height | 1.6 ± 0.3 (1.4-1.8) | 0.9 ± 0.1 (0.9-1.0) |
| Pharyngeal length | 102 ± 8.7 (97.9-110) | 109 ± 1.1 (108-110) |
| SE pore from anterior end | 75.4 ± 3.2 (74.0-77.0) | 77.9 ± 0.6 (77.5-78.5) |
| Mid-body diameter | 12.7 ± 1.6 (12.7-13.4) | 12.6 ± 1.1 (11.2-13.8) |
| Median bulb length | 9.1 ± 0.8 (8.6-9.8) | 8.0 ± 0.9 (7.4-8.6) |
| Median bulb diameter | 7.0 ± 0.2 (6.9-7.6) | 5.1 ± 0.3 (4.9-5.3) |
| Lip region diameter | 3.8 ± 0.8 (3.1-4.2) | 3.5 ± 0.2 (3.4-3.5) |
| Lip region height | 2.4 ± 0.3 (2.2-2.6) | 2.1 ± 0.5 (1.6-2.7) |
| Tail | 21.4 ± 0.8 (18.5-25.3) | 23.1 ± 2.0 (20.3-25.0) |
| Hyaline tail (h) | 2.5 ± 0.4 (1.3-3.1) | 3.4 ± 0.5 (2.9-3.9) |
| Spicule length | – | 16.7 ± 1.2 (16.0-17.5) |
| Gubernaculum length | – | 5.6 ± 0.5 (5.0-6.0) |
Comparison of important morphological characters and morphometrics of the Tanzanian Tylenchorhynchus crassicaudatus, found from sugarcane field F10-South of Tanganyika Planting Company Limited in Kilimanjaro region of Tanzania, with the original measurements of T_ crassicaudatus from Mauritius and T_ agri from the USA along with three other populations of T_ agri and T_ crassicaudatus from Niger, USA and Haiti_
| Character | T. crassicaudatus from cane roots in Mauritius (Williams, 1960a, b) | T. agri from corn field in Urbana, Illinois, USA (Ferris, 1963) | T. crassicaudatus from Niger (2011) | T. agri from dwarf date palm in Lake Worth, Florida, USA (Handoo et al., 2014) | T. crassicaudatus from sugarcane field in Kilimanjaro, Tanzania (2017) | T. agri from rice field in Haiti (2018) |
|---|---|---|---|---|---|---|
| n | 5 | 10 | 6 | 1 | 5 | 10 |
| Body length | 580-690 | 660-770 | 521-666 | 662 | 725-755 | 538-695 |
| a | 28-33 | 28-33 | 27-31 | 32.9 | 28-35 | 23-32 |
| b | 4.9-5.7 | 4.7-5.5 | 4.3-5.0 | 4.9 | 4.7-5.2 | 4.4-5.3 |
| c | 14-15 | 15-21 | 13-16 | 13.3 | 14-17 | 12-15 |
| V% | 53-57 | 55-58 | 53-59 | 55.1 | 53-55 | 51-55 |
| Stylet length | ca 20 | 20-23 | 18-20 | 21 | 19-20 | 18-19 |
| Tail length | ca 50 | – | 37-42 | 50 | 43-56 | 43-53 |
| Tail annuli number | 17-19 | 18-26 | 17-23 | 25 | 21-26 | 18-20 |
| Tail shape | Straight to slightly arcuate, thick and clavate | Subcylindrical | Clavate to subcylindrical | Subcylindrical | Subcylindrical | Subcylindrical |
| Tail terminus | Smooth, rounded and virtually hemispherical | Broadly rounded and smooth | Smooth, rounded and virtually hemispherical | Smooth and broadly rounded | Smooth and rounded | Broadly rounded and smooth |
| Post anal intestinal sac | Not mentioned (absent?) | Present | Absent | Not mentioned | Not present | Present or absent |
| Labial region | Broadly rounded, low, not setoff | Separated from body contour by slight depression | Broadly rounded, low, not set off, sometimes seems separated from body contour by slight depression | Continuous with body contour or separated by slight depression | Broadly rounded, not set off, sometimes seems separated from body contour by slight depression | Seems separated from body contour by slight depression |
| Labial annuli | 3 | 3 + labail disc | 3 | 3 to 4 | 3 | 3 |
Comparison of important morphological characters and morphometrics of immature females of the Tanzanian Rotylenchulus parvus found from sugarcane field F13-South of Tanganyika Planting Company Limited in Kilimanjaro region of Tanzania, with seven other Rotylenchulus spp_ and the original measurements of R_ parvus from Mauritius and from South Africa_
| Character | R. clavicaudatus from South Africa after Van den Berg et al. (2016) | R. leptus from South Africa after Van den Berg et al. (2016) | R. macrodoratus from Italy after Van den Berg et al. (2016) | R. macrosoma from Spain after Castillo et al. (2003a) | R. macrosomoides from South Africa after Van den Berg et al. (2016) | R. parvus (=Helicotylenchus parvus) from Mauritius after Williams (1960a, b) | R. parvus from South Africa after Van den Berg (1978) | R. parvus from Kilimanjaro, Tanzania (2017) | R. reniformis after Agudelo et al. (2005) | R. sacchari from South Africa after Van den Berg et al. (2016) |
|---|---|---|---|---|---|---|---|---|---|---|
| n | 11 | 14 | 9 | 12 | 10 | 6 | 198 | 28 | 20 | 25 |
| Body length | 483-624 | 321-434 | 407-489 | 408-510 | 463-590 | 210-270 | 231-432 | 271-352 | 340-560 | 574-796 |
| a | 27.6-36.9 | 25.7-29.9 | 22.6-27.4 | 26.3-34.2 | 31.4-37.3 | 19.0-24.0 | 15.3-32.3 | 21.9-26.8 | 20.3-31.9 | 28.8-36.9 |
| b | 3.8-4.7 | 2.9-4.3 | 2.9-4.0 | 3.5-4.4 | 3.8-4.7 | 2.9-3.3 | 2.0-4.4 | 2.6-3.9 | 2.3-4.1 | 3.1-5.0 |
| c | 9.1-11.8 | 14.5-17.3 | 18.5-22.2 | 11.7-16.8 | 14.1-21.4 | 16.0-20.0 | 13.1-27.1 | 12.3-17.5 | 10.0-22.8 | 26.1-43.0 |
| DGO | 11.0-18.0 | 20.0-24.5 | 13.0-18.0 | 22.0-27.0 | 30.0-33.0 | – | 7.7-17.3 | 16.6-18.0 | – | 8.0-15.5 |
| V% | 55-61 | 59-65 | 62-67 | 59-64 | 76-83 | 61-65 | 56-69 | 60-66 | 66-73 | 62-71 |
| Stylet length | 16.0-20.0 | 12.5-14.5 | 21.0-24.0 | 15.0-18.0 | 21.5-25.5 | ca 12.5 | 10.7-19.9 | 13.1-15.4 | 16.0-22.0 | 26.5-34.5 |
| Tail length | 43.0-66.0 | 20.0-28.0 | 20.0-24.0 | 26.0-40 | 26.5-36.0 | – | 10.5-27.2 | 18.5-25.3 | – | 15.5-28.0 |
| Hyaline length | 19.0-35.5 | 1.4-4.0 | 8.0-12.0 | 9.0-12.0 | 17.0-25.5 | – | 0.8-5.2 | 1.3-3.1 | 4.0-9.0 | 4.5-10.5 |
| Tail shape and structure | Broadly rounded, clavate, annulated tip | Tapering gradually to a finely rounded annulated tip | Bluntly rounded and slightly annulated terminus | Bluntly rounded and prominently annulated terminus | Tapering to a rounded or slightly clavate annulated tip | Arcuate, conoid, terminus knob like, length twice anal body diameter | Conoid, ventrally arcuate with sharply pointed or irregular or broadly rounded tip | Conoid, tapering to rounded tip | Tapering to rounded terminus | Broadly rounded, faint annulation at the tip |
| Habitus | Open letter C to complete circle | Open Figure 6 to 1.5 circle | Closed C-shape | Closed C-shape | C to curved into 1.5 circles | Loose spiral resembling 6-shape | C- or 6-shape or complete circle | C- to 6-shape | Open spiral to C-shape | Almost straight to C-shape rarely 1.5 circles |
| Labial region | Slightly sloping anteriorly to a slightly rounded tip, not set off | Sloping to a flattened tip | Conoid rounded, not set off | Conoid rounded, not set off | High, sloping slightly to a slightly flat or rounded tip | Continuous with neck, sloping to a rounded, distinctly flattened front | Broadly rounded, almost flat, not set off | Low, not set off and flattened front | High, conoid and continuous | Slightly sloping to flattened tip |
| Labial annuli | Not present | 4 to 5 | Fine annuli | Fine annuli | Not visible | – | 3-5 faint annuli | 4 to 5 faint annuli | 4 to 6 | 5 to 6 faint annuli |