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Mitochondrial COI gene is valid to delimitate Tylenchidae (Nematoda: Tylenchomorpha) species

Journal of Nematology
Volume 52 (2020): Issue 1 (January 2020)
By:
Open Access
|Apr 2020

Figures & Tables

Figure 1:

SEM pictures of Lelenchus leptosome population 1, 2 (de Man, 1880; Andrássy, 1954). (A, B) Lelenchus leptosome population 1; (C-J) Lelenchus leptosome population 2. (A, B, C) lip region; (D) anterior body (excretory pore indicated by arrow); (E) lateral view of the vulva; (F) excretory pore; (G) annulation at mid-body; (H) lateral view of cloacal aperture; (I) anus; (J) tail. (Scale bars: A, B, C, E, G=1 μm; D, H, J=10 μm ; F, I=2 μm).
SEM pictures of Lelenchus leptosome population 1, 2 (de Man, 1880; Andrássy, 1954). (A, B) Lelenchus leptosome population 1; (C-J) Lelenchus leptosome population 2. (A, B, C) lip region; (D) anterior body (excretory pore indicated by arrow); (E) lateral view of the vulva; (F) excretory pore; (G) annulation at mid-body; (H) lateral view of cloacal aperture; (I) anus; (J) tail. (Scale bars: A, B, C, E, G=1 μm; D, H, J=10 μm ; F, I=2 μm).

Figure 2:

LM pictures of Lelenchus leptosome populations 1 and 2. (K-N) Lelenchus leptosome population 1; (A-J) Lelenchus leptosome population 2. (A, B, K) body habitus; (C) anterior body; (D) ventral view of the vulva; (E) pharyngeal bulb; (F) lateral view of female reproductive system; (G-I, L, M) different image planes of cephalic region; (J) spicule and gubernaculum; (N) vulval to the anus. (Scale bar: 10 μm).
LM pictures of Lelenchus leptosome populations 1 and 2. (K-N) Lelenchus leptosome population 1; (A-J) Lelenchus leptosome population 2. (A, B, K) body habitus; (C) anterior body; (D) ventral view of the vulva; (E) pharyngeal bulb; (F) lateral view of female reproductive system; (G-I, L, M) different image planes of cephalic region; (J) spicule and gubernaculum; (N) vulval to the anus. (Scale bar: 10 μm).

Figure 3:

Line drawing of Lelenchus leptosoma population 2. (A, C, D, F) female; (B, E) male; (A, B) body habitus; (C) tail; (D) cephalic region; (E) spicule and gubernaculum; (F) anterior body; (G) reproductive system.
Line drawing of Lelenchus leptosoma population 2. (A, C, D, F) female; (B, E) male; (A, B) body habitus; (C) tail; (D) cephalic region; (E) spicule and gubernaculum; (F) anterior body; (G) reproductive system.

Figure 4:

Bayesian 50% majority rule consensus tree interfered with mitochondrial COI gene. New sequences original to this study are indicated in bold. Branch support is PP value in BI analysis.
Bayesian 50% majority rule consensus tree interfered with mitochondrial COI gene. New sequences original to this study are indicated in bold. Branch support is PP value in BI analysis.

Figure 5:

The maximum likelihood tree interfered on the mitochondrial COI gene. New sequences original to this study are indicated in bold. Branch support is BS value from ML analysis.
The maximum likelihood tree interfered on the mitochondrial COI gene. New sequences original to this study are indicated in bold. Branch support is BS value from ML analysis.

Figure 6:

Bayesian 50% majority rule consensus tree interfered with the 18S rRNA gene. New sequences original to this study are indicated in bold. Branch support is indicated in the following order: PP value in BI analysis/BS value from ML analysis.
Bayesian 50% majority rule consensus tree interfered with the 18S rRNA gene. New sequences original to this study are indicated in bold. Branch support is indicated in the following order: PP value in BI analysis/BS value from ML analysis.

Figure 7:

Bayesian 50% majority rule consensus tree interfered with the 28S rRNA gene. New sequences original to this study are indicated in bold. Branch support is indicated in the following order: PP value in BI analysis/BS value from ML analysis.
Bayesian 50% majority rule consensus tree interfered with the 28S rRNA gene. New sequences original to this study are indicated in bold. Branch support is indicated in the following order: PP value in BI analysis/BS value from ML analysis.

The compositional bias (GC content) and 1st, 2nd, and 3rd codon position nucleotide alignments_

Taxa
Nucleotide compositionTylenchidaeCriconematinaHoplolaimina
GC28.7222.5429.42
GC 1st39.8028.0738.82
GC 2nd35.1435.0736.61
GC 3rd11.234.4812.84

The p-distance of COI gene between studied Tylenchidae species_

LFJLFZCRAGBABTPHCCFVLL1LL2MBTALB
LFJ99.5
LFZ78.999.5
CR83.081.4100
AG83.281.399.899.5
BA79.876.281.581.399.5
BT78.776.682.482.379.499.8
PH72.573.577. 877.773.277.2100
CC80.578.287.587.379.880.875.798.6
FV82.479.583.383.280.982.576.382.4100
LL176.876.881.081.171.773.970.877.675.196.0
LL279.281.284.484.275.579.075.182.880.186.997.7
MB81.178.882.682.578.181.874.881.082.774.079.699.7
TA82.479.288. 988.881.885.678.583.783.876.781.384.7100
LB84.181.286.687.081.581.572.987.284.377.984.684.084.90

List species examined in this study and their corresponding sampling locations_

SpeciesGPS coordinatesAl.Vegetation environment
Labrys fujianensis 26°04´52.9˝N,119°14´26.7˝E28Scrubland soil with ferns and bamboo
Labrys fuzhouensis 26°08´57.6˝N,119°17´34.4˝E107Rhizosphere of Alpinia zerumbet
Coslenchus rafiqi 26°05´08.2˝N,119°14´10.0˝E27Swamp soil
Coslenchus costatus 26°05´00.9˝N, 119°14´32.6˝E25Rhizosphere soil of bamboo
Boleodorus thylactus 26°08´57.6˝N,119°17´34.4˝E107Rhizosphere soil of Alpinia zerumbet
Aglenchus geraerti 26°09´09.2˝N,119°17´35.7˝E88Rhizosphere soil of grass near the bamboo
Basiria aberrans 26°09´56.3˝N,117°55´34.2˝E644Rhizosphere soil of peanut
Filenchus vulgaris 26°05´00.9˝N,119°14´32.6˝E25.Rhizosphere soil of bamboo
Lelenchus leptosoma 126°05´00.9˝N,119°14´32.6˝E25.Rhizosphere soil of bamboo
Lelenchus leptosoma 226°08´57.3˝N,119°17´34.1˝E107Rhizosphere soil of Litchi chinensis
Malenchus bryanti 43°48´53.1˝N,125°24´40.3˝E225Rhizosphere soil of aspen
Tylenchus arcuatus 26°05´23.9˝N,119°14´00.3˝E12Rhizosphere soil of locust tree
Psilenchus hilarulus 26°05´09.4˝N,119°13´50.2˝E7Rhizosphere soil of grass
DOI: https://doi.org/10.21307/jofnem-2020-038 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 12
Submitted on: Jan 3, 2020
Published on: Apr 24, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Phylogeny,
28S rRNA,
18S rRNA,
Species identification
Related subjects:
Life sciences,
Life sciences, other

© 2020 Mengxin Bai, Xue Qing, Kaikai Qiao, Xulan Ning, Shun Xiao, Xi Cheng, Guokun Liu, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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