Have a personal or library account? Click to login

Genetic intraspecific diversity of Meloidogyne javanica parasitizing vegetables in southern Iran

Journal of Nematology
Volume 52 (2020): Issue 1 (January 2020)
By:
Open Access
|Apr 2020

Figures & Tables

Figure 1:

Species-specific 670 bp band in the populations of Meloidogyne javanica recovered from southern Iran. (For population codes, see Table 1).
Species-specific 670 bp band in the populations of Meloidogyne javanica recovered from southern Iran. (For population codes, see Table 1).

Figure 2:

Species-specific 1200 bp band in the populations of Meloidogyne incognita recovered from southern Iran. (For population codes, see Table 1).
Species-specific 1200 bp band in the populations of Meloidogyne incognita recovered from southern Iran. (For population codes, see Table 1).

Figure 3:

RAPD (left) and ISSR (right) patterns for 19 populations of Meloidogyne javanica generated with the primer (CCA)5 and OPAD10. DNA Ladder = 200 bp. (For population codes, see Table 1).
RAPD (left) and ISSR (right) patterns for 19 populations of Meloidogyne javanica generated with the primer (CCA)5 and OPAD10. DNA Ladder = 200 bp. (For population codes, see Table 1).

Figure 4:

UPGMA cluster diagram based on Dice coefficient estimated from 180 RAPD markers for 37 populations of Meloidogyne javanica (For population codes, see Table 1).
UPGMA cluster diagram based on Dice coefficient estimated from 180 RAPD markers for 37 populations of Meloidogyne javanica (For population codes, see Table 1).

Figure 5:

UPGMA cluster diagram based on Dice coefficient estimated from 164 ISSR markers for 37 populations of the Meloidogyne javanica (For population codes, see Table 1).
UPGMA cluster diagram based on Dice coefficient estimated from 164 ISSR markers for 37 populations of the Meloidogyne javanica (For population codes, see Table 1).

Figure 6:

A Mantel test showing correlation between RAPD and ISSR datasets of the populations of Meloidogyne javanica recovered from southern Iran.
A Mantel test showing correlation between RAPD and ISSR datasets of the populations of Meloidogyne javanica recovered from southern Iran.

Average of the morphometrics of females, J2s and males of five populations of Meloidogyne javanica parasitizing vegetables in southern Iran_

Females Males Second-stage juveniles (J2s)†
Diagnostic characters15CV10CVMJ1MJ4MJ8MJ14MJ1550CV
L (including neck)686±127 (506-1,010)181482±155 (1189-1,694)10463457449482469464±19 (429-497)4
Body width (W)420±99 (274-568)2333±2.8 (29-38)9141415151615±1.5 (12-18)9
Neck length259±87 (153-540)33−−−−−−−−−
Stylet15±2 (13-18)1418±1.2 (16-19)69.710.410.510.110.210±0.5 (9-12)5
DGO3.1±0.6 (2.0-3.9)203.3±0.2 (3.1-3.5)52.72.12.21.92.02.2±0.5 (1.5-3.7)22
Excretory pore190±30 (145-270)16−−−−−−−−−
Vulval slit length21±3 (15-27)15−−−−−−−−−
Vulva-anus distance16±4 (12-28)25−−−−−−−−−
Inter-phasmidial distance78 ± 16 (58-108)20−−−−−−−−−
Tail length−−14±2.1 (10-17)15515555585755±5 (40-66)9
Hyaline length−−−−111211161613±4 (7-20)27
Pharynx (glands end)−−473±50 (390-552)11164202197201201193±23 (90-216)12
Body length to width (a)1.7±0.4 (1.2-2.6)2245±4.5 (37-51)10383229333031±3 (25-39)10
b−−6.6±1.3 (4.8-8.1)206.44.94.75.05.25.2±1.4 (4.3-12.4)27
b'−−3.1±0.6 (2.4-3.9)183.02.32.32.42.32.5±0.5 (2.0-5.2)22
c−−113±26 (87-169)239.48.48.28.38.28.5±0.8 (7.2-11.3)10
c'−−0.5±0.1 (0.3-1.0)194.65.35.55.95.25.7±0.5 (4.7-7.2)10
Spicules−−23±5.8 (15-29)24−−−−−−−
Gubernaculum−−13±2.8 (8-17)20−−−−−−−

Nei’s (1978) unbiased genetic distance between pairs of Meloidogyne javanica populations detected by RAPD and ISSR analyses_

Population† AbdanDeirBardkhonJiroftBeizaKhafrKazeron
Abdan–0.0320.0700.0740.0910.0840.088
Deir0.082–0.0540.0760.0680.0880.091
Bardkhon0.0900.076–0.0460.0450.0390.059
Jiroft0.0690.1270.119–0.0710.0400.025
Beiza0.0430.0800.1190.108–0.0730.071
Khafr0.0800.0550.1430.1340.075–0.051
Kazeron0.1200.0710.0500.1560.1320.162–

Analysis of molecular variance (AMOVA) of Meloidogyne javanica populations based on RAPD and ISSR data_

MarkerSource of variationDegree of freedomSum of squaresVariance componentsPercentage of variationFixation index (F ST )† Number of migrants (Nm)
Between populations6148.882.63*19
RAPDWithin populations30340.8511.36*810.1881.079
Total36489.7313.99
Between populations690.681.34*14
ISSRWithin populations30247.808.26*860.1401.535
Total36338.499.60

Genetic diversity estimates of Meloidogyne javanica populations recovered from southern Iran by RAPD and ISSR analyses_

ISSR RAPD
Population%P† HI%PHI
Abdan (10)†† 25.610.1100.15836.110.1500.218
Deir (5)20.730.0920.13030.000.1320.189
Bardkhon (7)24.390.0970.14130.560.1180.173
Jiroft (3)10.370.0390.05817.780.0790.113
Beiza (5)21.340.0880.12822.780.0950.137
Khafr (4)18.290.0760.11016.110.0640.093
Kazeron (3)12.800.0570.08118.890.0790.114
Mean19.080.0800.11524.600.1030.148

Populations of Meloidogyne species collected in this study_

NumberSampling areaNESampleHost plantMolecular code
1Boshehr province-Abdan28°11´00.38˝51°74´07.94˝Soil and rootTomatoMJ1
2Boshehr province-Abdan28°10´99.86˝51°74´08.46˝Soil and rootTomatoMJ2
3Boshehr province-Abdan28°11´00.58˝51°74´07.97˝Soil and rootTomatoMJ3
4Boshehr province-Abdan28°11´00.29˝51°74´08.27˝Soil and rootTomatoMJ4
5Boshehr province-Abdan28°11´00.31˝51°74´08.28˝Soil and rootTomatoMJ5
6Boshehr province-Abdan28°10´95.09˝51°74´04.66˝Soil and rootTomatoMJ6
7Boshehr province-Abdan28°10´94.93˝51°74´04.44˝Soil and rootTomatoMJ7
8Boshehr province-Abdan28°10´94.82˝51°74´04.43˝Soil and rootTomatoMJ8
9Boshehr province-Abdan28°10´94.56˝51°74´03.85˝Soil and rootTomatoMJ9
10Boshehr province-Abdan28°10´94.47˝51°74´03.81˝Soil and rootTomatoMJ10
11Boshehr province-Deir Port27°91´15.43˝51°98´02.27˝Soil and rootTomatoMJ11
12Boshehr province-Deir Port27°91´15.16˝51°98´02.71˝Soil and rootTomatoMJ12
13Boshehr province-Deir Port27°91´17.39˝51°98´03.58˝Soil and rootTomatoMJ13
14Boshehr province-Deir Port27°91´17.33˝51°98´03.47˝Soil and rootTomatoMJ14
15Boshehr province-Deir Port27°91´44.84˝51°98´43.36˝Soil and rootTomatoMJ15
16Boshehr province-Bardkhon28°07´54.24˝51°48´89.26˝Soil and rootTomatoMJ16
17Boshehr province-Bardkhon28°07´51.78˝51°48´90.21˝Soil and rootTomatoMJ17
18Boshehr province-Bardkhon28°36´13.93˝51°48´90.22˝Soil and rootTomatoMJ18
19Boshehr province-Bardkhon28°07´51.25˝51°48´84.44˝Soil and rootTomatoMJ19
20Boshehr province-Bardkhon28°07´51.92˝51°48´82.48˝Soil and rootTomatoMJ20
21Boshehr province-Bardkhon28°07´52.13˝51°48´82.42˝Soil and rootTomatoMJ21
22Boshehr province-Bardkhon28°07´52.15˝51°48´82.19˝Soil and rootTomatoMJ22
23Kerman province-Jiroft28°41´09.00˝57°40´13.81˝RootMallowMJ23
24Kerman province-Jiroft28°41´35.08˝57°39´25.18˝RootGoosefootMJ24
25Kerman province-Jiroft28°42´22.57˝57°43´05.86˝RootEggplantMJ25
26Fars province-Beyza29°55´30.53˝52°26´28.41˝Soil and rootCucumberMJ26
27Fars province-Beyza29°55´51.85˝52°26´44.88˝Soil and rootCucumberMJ27
28Fars province-Beyza29°55´59.49˝52°26´51.00˝Soil and rootCucumberMJ28
29Fars province-Beyza29°55´16.00˝52°27´20.15˝Soil and rootCucumberMJ29
30Fars province-Beyza29°55´11.72˝52°27´27.93˝Soil and rootCucumberMJ30
31Fars province-khafr28°57´44.57˝53°11´58.46˝Soil and rootOkraMJ31
32Fars province-khafr28°57´35.80˝53°12´32.31˝Soil and rootOkraMJ32
33Fars province-khafr28°57´34.97˝53°12´28.38˝Soil and rootOkraMJ33
34Fars province-khafr28°58´56.08˝53°11´47.87˝Soil and rootOkraMJ34
35Fars province-Kazeron29°33´23.23˝51°45´56.27˝Soil and rootTomatoMJ35
36Fars province-Kazeron29°33´25.22˝51°45´42.76˝Soil and rootTomatoMJ36
37Fars province-Kazeron29°33´42.68˝51°45´08.64˝Soil and rootTomatoMJ37
38Fars province-Fasa29°00´46.56˝53°37´42.27˝Soil and rootCucumberMI1
39Fars province-Fasa29°00´46.56˝53°37´42.27˝Soil and rootCucumberMI2
40Fars province-Fasa29°00´46.56˝53°37´42.27˝Soil and rootCucumberMI3
41Fars province-Fasa29°00´46.56˝53°37´42.27˝Soil and rootCucumberMI4

All primers used for ISSR, RAPD and species-specific identification of Meloidogyne species during the present study_

ISSRRAPD
(CCA)5 5´-CCACCACCACCACCA-3'OPA85’-GTGACGTAGG-3'
(GTG)6 5'-GTGGTGGTGGTGGTGGTG-3'OPA105'-GTGATCGCAG-3'
(ATG)6 5'-ATGATGATGATGATGATG-3'OPA135'-CAGCACCCAC-3'
(GAG)4GC5'-GAGGAGGAGGAGGC-3'OPAD105'-AAGAGGCCAG-3'
(GACA)4 5'-GACAGACAGACAGACA-3'OPE185'-GGACTGCAGA-3'
(GA)8C5'-GAGAGAGAGAGAGAGAC-3'OPP175'-TGACCCGCCT-3'
(AC)8T5'-ACACACACACACACACT-3'OPB115'-GTAGACCCGT-3'
(CA)8G5'-CACACACACACACACAG-3'OPB175'-AGGGAACGAG-3'
(CTC)4GC5'-CTCCTCCTCCTCGC-3'OPD85'-GTGTGCCCCA-3'
(GAGA)4GG5'-GAGAGAGAGAGAGAGAGG-3'OPC85'-TGGACCGGTG-3'
Species-specific primers
M. javanica Fjav (5'-GGTGCGCGATTGAACTGAGC-3') M. incognita Finc (5'-CTCTGCCCAATGAGCTGTCC-3')
Rjav (5'-CAGGCCCTTCAGTGGAACTATAC-3') Rinc (5'-CTCTGCCCTCACATTAAG-3')
M. arenaria Far (5'-TCGGCGATAGAGGTAAATGAC-3') M. hapla F (5'- TGACGGCGGTGAGTGCGA-3')
Rar (5'-TCGGCGATAGACACTACAAACT-3') R (5'-TGACGGCGGTACCTCATAG-3')
DOI: https://doi.org/10.21307/jofnem-2020-035 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Journal RSS Feed
Language: English
Page range: 1 - 13
Submitted on: Oct 30, 2019
Published on: Apr 23, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Diversity,
ISSR,
RAPD,
root-knot nematodes,
species-specific primer
Related subjects:
Life sciences,
Life sciences, other

© 2020 Reza Ghaderi, Ali Asghar Dehghan, Abbas Mokaram Hesar, Akbar Karegar, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 52 (2020): Issue 1 (January 2020)Next article