Figure 1:
Figure 2:
Figure 3:
Figure 4:
Figure 5:
Figure 6:
Morphometric data for Acrobeloides saeedi DH1 isolated from Galleria culture_
| Characters | Female | Male | Juveniles |
|---|---|---|---|
| n | 20 | 20 | 27 |
| Total body length | 1271 ± 112 (1060–1446) | 959 ± 74 (798–1144) | 474 ± 54 (404–636) |
| a | 14.1 ± 1.3 (11.5–16.6) | 20 ± 1.8 (17.1–24.0) | 21 ± 3.5 (15.3–28.0) |
| b | 6.9 ± 0.4 (6.1–7.8) | 5.0 ± 0.3 (4.2–5.6) | 3.8 ± 0.36 (3.2–4.9) |
| c | 27 ± 2.2 (22.0–30.0) | 27 ± 3.0 (22–34) | 13 ± 2.3 (5.7–17.6) |
| c' | 1.7 ± 0.2 (1.5–2.4) | 1.6 ± 0.1 (1.3–1.8) | 2.7 ± 0.7 (2.0–6.1) |
| V | 71 ± 3.6 (60–77) | – | – |
| Lip length | 4.8 ± 0.7 (3–6) | 3.8 ± 0.5 (3–5) | 2.8 ± 0.5 (2–4) |
| Lip region width | 7.8 ± 1.4 (5–10) | 6.3 ± 0.7 (5–8) | 5.1 ± 0.7 (4–7) |
| Stoma length | 13.1 ± 1.2 (11–16) | 12.2 ± 1.6 (8–15) | 10.4 ± 1.9 (8–15) |
| Pharyngeal corpus | 107 ± 9.8 (86–125) | 113 ± 6.7 (99–123) | 85 ± 8.8 (64–100) |
| Isthmus | 26 ± 4.5 (16–34) | 28 ± 4.4 (21–36) | 18.2 ± 4.0 (9–27) |
| Basal bulb length | 37 ± 3.6 (30–43) | 34 ± 4.1 (26–40) | 21 ± 2.9 (16–30) |
| Pharynx length | 170 ± 10.3 (155–188) | 174 ± 8.4 (146–184) | 122 ± 11.9 (105–142) |
| Nerve ring – ant. end | 112 ± 6.6 (98–124) | 131 ± 8.0 (114–143) | 82 ± 9.1 (65–102) |
| Excretory pore – ant. end | 134 ± 12.6 (115–161) | 157 ± 12.5 (141–192) | 95 ± 11.8 (77–123) |
| Deirid – ant. end | 127 ± 17.3 (95–159) | 125 ± 7.5 (105–139) | ? |
| Neck length | 184 ± 10.2 (170–202) | 191 ± 8.1 (175–207) | 125 ± 9.4 (108–153) |
| Body diam. at midbody | 90 ± 10.9 (70–108) | 47 ± 3.1 (40–54) | 23 ± 3.4 (18–31) |
| Ovary length | 437 ± 51 (348–532) | – | – |
| Spermatheca length | 50 ± 10.4 (39–68) | – | – |
| Uterus length | 385 ± 85 (256–537) | – | – |
| Postvulval uterine sac length | 92 ± 8.6 (73–101) | – | – |
| Vagina length | 23 ± 1.83 (19–25) | – | – |
| Body diam. at vulva level | 75 ± 10.8 (56–91) | – | – |
| Vulva – anterior end | 904 ± 87 (749–1043) | – | – |
| Rectum length | 33 ± 4.8 (22–43) | – | 13.4 ± 2.2 (10–18) |
| Body diam. at anus | 28 ± 2.4 (21-32) | 23 ± 2.7 (20-28) | 14.3 ± 1.6 (11-17) |
| Tail length | 48 ± 3.8 (41-54) | 36 ± 2.5 (32-40) | 38 ± 9.9 (29-80) |
| Phasmid to anus distance | 27 ± 4.0 (21-37) | 21 ± 2.1 (18–25) | – |
| Spicule length | – | 45 ± 2.8 (41-50) | – |
| Gubernaculum length | – | 26 ± 2.0 (22-30) | – |
Comparative morphometrics of males from populations of Acrobeloides maximus – group (all measurements in µm except L in mm)_
| Species | A. saeedi (KMW-DH1) | A. longiuterus | A. longiuterus (as camberenensis) | A. longiuterus (as A. camberenensis) | A. longiuterus | A. saeedi (as A. gossypii) | A. saeedi (as A. gossypii) | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri (as A. mushtaqi) | A. maximus |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Reference | Present paper | Rashid and Heyns (1990) | De Ley et al. (1990) | De Ley et al. (1999) | Abolafia and Peña-Santiago, 2017a, b | Nahiyoon et al. (2019) | Nahiyoon et al. (2019) | Steiner (1936); Siddiqi et al. (1992) | Andrássy (1967) | Siddiqi et al. (1992) | De Ley et al. (1999) | Abolafia, Peña-Santiago (2002) | Mehdizadeh et al. (2013) | Pervez (2011) | Steiner (1935) |
| Country (State) | India | Namibia | Senegal | USA | Namibia | Pakistan | Pakistan | Israel | Mongolia | Malawi | USA | Spain | Iran | India | USA |
| n (♂) | 40 | 26 | 6 | 20 | 6 | 15 | 15 | 8 | 6 | 10 | 10 | 2 | 4 | 10 | 1 |
| L | 0.79–1.16 | 0.53–0.94 | 0.54–0.65 | 0.70–1.03 | 0.68–0.84 | 0.71–1.39 | 0.71–1.39 | 0.63–0.71 | 0.56–0.59 | 0.56–0.87 | 0.98–1.18 | 0.69, 0.73 | 0.70–0.86 | 0.61–0.62 | 0.9 |
| a | 13.0–24.0 | 16.7–26.4 | 20.2–21.7 | 16.0–26.0 | 17.8–23.7 | 11.1–16.6 | 11.1–16.6 | 14.0–16.0 | 12.0–15.0 | 22–26 | 20.0–25.0 | 24.0, 25.0 | 22.0–30.0 | 27.0–31.0 | 27.0 |
| b | 4.2–7.0 | 4.0–5.9 | 4.0–4.8 | 4.3–6.8 | 4.1–5.1 | 4.6–7.5 | 4.6–7.5 | 4.9–5.1 | 4.2–4.8 | 4.3–5.4 | 6.0–7.8 | 5.0, 5.2 | 4.8–5.4 | 4.0–4.2 | 7.2 |
| c | 21.0–34.0 | 14.4–21.7 | 14.9–18.0 | 17.0–23.0 | 17.3–20.3 | 19.5–27.8 | 19.5–27.8 | 15–18 | 14.0–16.0 | 15–18 | 20.0–24.0 | 18.8, 16.6 | 14.0–18.0 | 16.0–18.0 | 18.0 |
| c’ | 1.2–2.2 | 1.4–2.1 | 1.6–2.0 | 1.2–1.6 | 1.2–1.5 | 0.9–1.3 | 0.9–1.3 | ? | 1.9* | 2.1* | 1.3–1.7 | 1.3, 1.7 | 1.6–2.0 | 1.4–1.8 | 1.8* |
| Lip region width | 5–8 | ? | ? | ? | ? | 11–14 | 11–14 | ? | ? | ? | ? | 3, 4 | 3–4 | ? | ? |
| Stoma length | 8–17 | ? | 08–10 | 11–13 | 11–12 | 12–15 | 12–15 | ? | ? | ? | 10–14 | 11, 12 | 10–11 | 11–12 | ? |
| Procorpus length | 28–59 | ? | ? | 58–66 | ? | ? | ? | ? | ? | ? | ? | 11, 11 | 12–14 | ? | ? |
| Metacorpus length | 45–75 | ? | ? | 32–40 | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? |
| Isthmus length | 21–50 | ? | 15–25 | 16–23 | 19–22 | 16–20 | 16–20 | 22–23 | ? | ? | ? | ? | 17–32 | ? | ? |
| Bulb length | 26–41 | ? | 16–23 | 17–27 | 22–28 | 24–30 | 24–30 | ? | ? | ? | 22–30 | 22, 23 | 20–26 | ? | ? |
| Pharynx length | 142–184 | ? | 122–151* | 205–260 | 122–161 | 150–188 | 150–188 | 129–143 | ? | ? | 210–293 | 100, 119 | 131–149 | 145–152 | ? |
| Nerve ring – ant. End | 88–143 | ? | 90–104 | 108–135 | 120–132 | 112–134 | 112–134 | 100–115 | ? | ? | 117–151 | 140, 140 | 110–127 | 106–110 | ? |
| Excretory pore – ant end | 112–192 | ? | 78–91 | 121–175 | 119–141 | 132–144 | 132–144 | ? | ? | ? | 135–192 | 121, ? | 128–143 | ? | ? |
| Neck length | 156–207 | 130–174 | 128–148 | ? | 156–168 | 162–203* | 162–203* | ? | ? | ? | 133–188 | 143–160 | ? | ? | |
| Midbody diam. | 40–73 | ? | 25–31 | 39–51 | 35–42 | 64–88 | 64–88 | ? | ? | ? | 44–57 | 33, 40 | 24–40 | 19–22 | ? |
| Anal body diam. | 18–34 | ? | 20–23 | 27–35 | 27–34 | 32–40 | 32–40 | ? | ? | 25* | 31–35 | 18, 22 | 24–27 | 17–24 | ? |
| Tail length | 32–48 | 37–50 | 36–42 | 39–47 | 38–44 | 40–50 | 40–50 | 38–43 | ? | 54–80 | 40–58 | 22, 25 | 43–54 | 34–37 | ? |
| Spicules length | 41–54 | 29–51 | 29–34 | 40–48 | 41–46 | 38–57 | 38–57 | 39–44 | 30–40 | 35–43 | 42–50 | 37 | 38–41 | 39–43 | ? |
| Gubernaculum length | 21–30 | 18–35 | 14–19 | 24–30 | 26–31 | 24–35 | 24–35 | 22–28 | 20 | 19–24 | 27–34 | 23, 22 | 19–28 | 23–24 | ? |
| D% (EP/ES×100) | 69–94 | ? | ? | 57–67* | 92* | 82* | 82* | ? | ? | ? | ? | 97* | ? | ? | |
| E% (EP/T×100) | 242–577 | ? | ? | 310–372* | 317* | 306* | 306* | ? | ? | ? | ? | 233, 300* | 279* | ? | ? |
| SW% (SL/ABD×100) | 150–294 | ? | ? | 151* | 143* | 126* | 126* | ? | ? | ? | ? | 137* | 155* | 194* | ? |
| GS% (GL/SL×100) | 40–70 | ? | ? | 67* | 66* | 55* | 55* | ? | ? | ? | ? | 65* | 59* | 58* | ? |
Pairwise distances of the D2D3 regions of 28 S rDNA regions between present strains of Acrobeloides and already described species_
| S. No. | 28S rDNA | Country | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | MK935147 A. saeedi KMW | India | 1 | 1 | 5 | 5 | 5 | 38 | 51 | 59 | 67 | 74 | 75 | 76 | 76 | |
| 2 | MN101167 A. saeedi DH1 | India | 100 | 0 | 5 | 5 | 5 | 38 | 51 | 59 | 68 | 74 | 75 | 77 | 77 | |
| 3 | KY914573 A. saeedi | Iran | 100 | 100 | 10 | 10 | 10 | 38 | 56 | 64 | 68 | 79 | 80 | 82 | 82 | |
| 4 | MF325168 A. sexlineatus | Germany | 98.9 | 98.9 | 98.0 | 0 | 0 | 5 | 8 | 0 | 11 | 0 | 0 | 0 | 0 | |
| 5 | MF325157 A. buchneri | Germany | 98.9 | 98.9 | 98.0 | 100 | 0 | 5 | 8 | 0 | 11 | 0 | 0 | 0 | 0 | |
| 6 | DQ903087 A. tricornis | Germany | 95.1 | 95.0 | 94.7 | 100 | 100 | 5 | 8 | 0 | 11 | 0 | 0 | 0 | 0 | |
| 7 | AF147069 A. longiuterus | USA | 96.7 | 96.7 | 96.8 | 99.0 | 99.0 | 96.9 | 30 | 35 | 45 | 34 | 35 | 36 | 36 | |
| 8 | AF147067 A. maximus | USA | 95.6 | 95.5 | 95.2 | 98.5 | 98.5 | 96.1 | 97.5 | 43 | 51 | 44 | 45 | 46 | 46 | |
| 9 | KX889089 A. varius | South Korea | 94.6 | 94.6 | 94.3 | 100 | 100 | 99.8 | 97.0 | 96.3 | 41 | 1 | 0 | 3 | 3 | |
| 10 | DQ145625 A. bodenheimeri | Belgium | 95.8 | 95.6 | 95.7 | 97.7 | 97.7 | 96.9 | 96.1 | 95.6 | 96.4 | 50 | 49 | 50 | 50 | |
| 11 | DQ903076 A. nanus | Sweden | 95.2 | 95.2 | 94.9 | 100 | 100 | 99.9 | 97.1 | 96.3 | 99.9 | 96.9 | 1 | 2 | 2 | |
| 12 | DQ903083 A. thornei | USA | 95.1 | 95.1 | 94.9 | 100 | 100 | 99.8 | 97.0 | 96.2 | 100 | 96.9 | 99.9 | 3 | 3 | |
| 13 | DQ145624 A. ellesmerensis | USA | 95.1 | 95.0 | 94.8 | 100 | 100 | 100 | 96.9 | 96.1 | 99.8 | 96.9 | 99.9 | 99.8 | 0 | |
| 14 | DQ903081 A. buetschlii | USA | 95.1 | 95.0 | 94.8 | 100 | 100 | 100 | 96.9 | 96.1 | 99.8 | 96.9 | 99.9 | 99.8 | 100 |
Morphometric data for Acrobeloides saeedi KMW isolated from Galleria culture_
| Characters | Female | Male | Juvenile |
|---|---|---|---|
| n | 20 | 20 | 27 |
| Total body length | 1387 ± 63 (1307–1566) | 987 ± 89 (812–1156) | 653 ± 20 (626–704) |
| a | 14.8 ± 1.4 (12.8–17.4) | 21 ± 2.7 (13.0–24.0) | 22 ± 1.7 (19.6–28.0) |
| b | 7.8 ± 0.7 (6.9–10.0) | 5.9 ± 0.6 (4.7–7.0) | 4.4 ± 0.2 (4.0–5.0) |
| c | 26 ± 2.2 (22.0–33.0) | 25 ± 2.7 (21.0–30.0) | 17 ± 1.2 (15.0–21.0) |
| c’ | 1.8 ± 0.2 (1.5–2.3) | 1.7 ± 0.2 (1.2–2.2) | 2.0 ± 0.2 (1.4–2.6) |
| V | 70 ± 2.1 (66–74) | – | – |
| Lip length | 5.0 ± 0.6 (4–7) | 4.6 ± 0.8 (3–6) | 4.3 ± 0.7 (3–6) |
| Lip region width | 8.9 ± 0.8 (8–11) | 6.3 ± 0.7 (5–8) | 5.0 ± 0.5 (4–5) |
| Stoma length | 12.9 ± 2.2 (9–15) | 14.6 ± 1.9 (11–17) | 12 ± 1.6 (7–14) |
| Pharyngeal corpus length | 108 ± 8.1 (88–124) | 93 ± 8.9 (81–108) | 82 ± 7.4 (69–99) |
| Isthmus length | 37 ± 4.4 (28–46) | 35 ± 6.0 (28–50) | 29 ± 4.4 (21–37) |
| Basal bulb length | 39 ± 4.9 (31–53) | 34 ± 3.4 (28–41) | 24 ± 1.5 (21–27) |
| Pharynx length | 184 ± 11.1 (159–202) | 161 ± 12.9 (142–181) | 136 ± 7.8 (116–149) |
| Nerve ring – ant. end | 113 ± 14 (91–151) | 106 ± 10.2 (88–129) | 94 ± 8.4 (76–109) |
| Excretory pore – ant. end | 138 ± 11.0 (112–157) | 129 ± 12.4 (112–165) | 111 ± 8.9 (94–127) |
| Deirid – ant. end | 155 ± 13.4 (130–178) | 128 ± 11.9 (111–156) | – |
| Neck length | 191 ± 9.7 (168–208) | 181 ± 12.8 (156–203) | 162 ± 12.4 (130–181) |
| Body diam. at midbody | 95 ± 10.0 (80–112) | 49 ± 7.7 (40–73) | 30 ± 2.1 (24–33) |
| Ovary length | 542 ± 60 (401–652) | – | – |
| Spermatheca length | 49 ± 9.8 (33–61) | – | – |
| Uterus length | 280 ± 46 (211–376) | – | – |
| Postvulval uterine sac length | 100 ± 11.3 (85–112) | – | – |
| Vagina length | 26 ± 6.2 (17–35) | – | – |
| Body diam. at vulval level | 84 ± 5.7 (74–93) | – | – |
| Vulva – anterior end | 976 ± 36 (896–1046) | – | – |
| Rectum length | 24 ± 5.5 (12–32) | – | 19.5 ± 2.1 (14–22) |
| Body diam. at anus | 31 ± 3.4 (22–39) | 24 ± 3.3 (18–34) | 20 ± 2.7 (16–31) |
| Tail length | 54 ± 4.1 (44–62) | 39 ± 3.5 (34–48) | 40 ± 2.9 (31–45) |
| Phasmid to anus distance | 30 ± 3.4 (26–35) | 28 ± 3.9 (21–36) | – |
| Spicules length | – | 48 ± 4.2 (41–54) | – |
| Gubernaculum length | – | 25 ± 2.8 (21–30) | – |
Comparative morphometrics of females from populations of Acrobeloides maximus – group (all measurements in µm except L in mm)_
| Species | A. saeedi (KMW-DH1) | A. saeedi | A. saeedi | A. saeedi (as A. ishraqi) | A. saeedi (as A. gossypii) | A. saeedi (as A. gossypii) | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri (as A. rotundifolius) | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri | A. bodenheimeri (as A. mushtaqi) | A. longiuterus | A. longiuterus (as A. camberenensis) | A. longiuterus (as A. camberenensis) | A. longiuterus | A. maximus | Paracephalobus litoralis |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Reference | Present paper | Siddiqi et al. (1992) | Khan and Hussain (1997) | Pervez (2011) | Nahiyoon et al. (2019) | Nahiyoon et al. (2019) | Steiner (1936); Siddiqi et al. (1992) | Andrássy (1967) | Bussau (1991) | Siddiqi et al. (1992) | De Ley et al. (1999) | Abolafia and Peña-Santiago (2002) | Mehdizadeh et al. (2013) | Pervez (2011) | Rashid and Heyns (1990) | De Ley et al. (1990) | De Ley et al. (1999) | Abolafia and Peña-Santiago, 2017a, b | Thorne (1925) | Akhtar (1962) |
| Country | India | Pakistan | Pakistan | India | Pakistan | Pakistan | Israel | Mongolia | Denmark | Malawi | USA | Spain | Iran | India | Namibia | Senegal | USA | Namibia | USA | Pakistan |
| n (♀) | 40 | 30 | 20 | 10 | 15 | 15 | 8 | 12 | 1 | 22 | 30 | 4 | 3 | 10 | 21 | 5 | 15 | 3 | 1 | 1 |
| L | 1.06–1.57 | 0.88–1.21 | 0.86–1.20 | 0.99–1.19 | 0.80–1.70 | 0.8–1.70 | 0.63–0.78 | 0.67–0.77 | 0.88 | 0.64–0.91 | 0.86–1.53 | 0.70–0.91 | 0.63–0.72 | 0.61–0.68 | 0.57–0.88 | 0.45–0.67 | 0.88–1.19 | 0.74–0.84 | 1.2 | 0.8 |
| A | 11.5–17.4 | 21.0–30.0 | 21.0–30.0 | 10.5–15.8 | 8.1–15.5 | 8.1–15.5 | 15.0–16.0 | 15.0–17.0 | 24.4 | 17–23 | 16.0–23.0 | 17.5–22.9 | 21.0–22.0 | 31.8–33.1 | 16.8–24.0 | 18.3–19.6 | 15.0–19.0 | 18.1–22.8 | 20.0 | 23.2 |
| B | 6.1–10.0 | 4.6–5.9 | 4.86–5.8 | 4.8–6.1 | 4.5–8.0 | 4.5–8.0 | 5.0–5.6 | 4.8–5.5 | 4.7 | 4.7–5.6 | 5.6–7.8 | 4.5–5.5 | 4.2–5.1 | 4.2–4.4 | 3.5–5.3 | 3.4–4.6 | 5.7–6.9 | 4.4–5.0 | 5.7 | 6.0 |
| C | 22.0–33.0 | 20.0–26.0 | 20–26 | 20.6–23 | 16.0–29.2 | 16–29.2 | 17–21 | 16.0–19.0 | 18.1 | 16.0–18.3 | 20.0–30.0 | 19.0–20.3 | 16.0–20.0 | 14.3–17.9 | 16.2–21.8 | 15.1–18.0 | 20.0–26.0 | 16.5–19.6 | 18.0 | 17.7 |
| c' | 1.5–2.4 | 1.8–2.4 | 1.8–2.3 | 1.4–1.8 | 1.1–1.9 | 1.1–1.9 | ? | 1.7–2.1 | 2.1* | 2.0–2.5 | 1.4–2.1 | 1.6–2.1 | 1.8–2.1 | 1.2–1.8 | 1.4–2.3 | 2.0–2.4 | 1.4–1.9 | 1.7–2.1 | 2.0 | 2.5–2.7* |
| V | 60–77 | 65–74 | ? | 68–74 | 70–75 | 70.2–75 | 69–71 | 65–71 | 69 | 67–71 | 67–71 | 64–69 | 67–70 | 77* | 64–72 | 65–70 | 84* | 66–70 | 70 | 65 |
| Lip region width | 5–11 | 11–13 | 11–13 | 11–13 | 9–10 | 12–15 | 9–10 | ? | 6.7* | 5.9* | 9* | 3 | 10–11 | 8.6–12 | ? | ? | ? | 5.5* | ? | 7* |
| Stoma length | 9–18 | 12* | 12–18 | 16–16 | 12–14 | 12–22 | 12–14 | ? | 20* | 14.7* | 11–16 | 11–14 | 13–14 | 8–12 | 11–14 | 8–10 | 12–15 | 10–12 | 20 | 10* |
| Procorpus | 37–69 | ? | ? | ? | ? | ? | ? | ? | ? | ? | 50–79 | ? | ? | ? | ? | ? | 60–85 | ? | ? | ? |
| Metacorpus | 43–80 | ? | ? | ? | ? | ? | ? | ? | ? | ? | 30–49 | ? | ? | ? | ? | ? | 35–46 | ? | ? | ? |
| Isthmus | 16–46 | 21.9* | 20* | ? | 20–26 | 14–28 | 20–26 | ? | 23* | 22–36 | 20–37 | 22–25 | 21–25 | 27.6* | 14–15* | 15–24 | 17–23 | 15 –23 | ? | ? |
| Bulb length | 30–53 | 29–34 | 29–34 | 24–38 | 19–21 | 26–32.5 | 19–21 | ? | 19.8* | 19–21 | 22–32 | 22–27 | 19–26 | 24* | ? | 15–21 | 22–28 | 24–25 | ? | 13–25 |
| Pharynx length | 155–202 | 190–218 | 190–218 | 179–223 | 129–157 | 166–228 | 129–157 | ? | 157* | 135–178 | 205–316* | 135–166 | 133–141 | 145–154 | ? | 123 –159 | 229–313* | 137–164 | 210 | 8–14 |
| Nerve ring–ant. end | 91–151 | 163* | ? | 151* | 92–114 | 125–136 | 92–114 | ? | 132* | 106* | 113–174 | 107–126 | 109–117 | 101–105 | 89–142 | 82–108 | 125–148 | 119–135 | 162 | 78* |
| Excretory pore–ant. end | 112–161 | 140–188 | 140–188 | 155–174 | ? | 136–150 | ? | ? | 139* | 118–134 | 131–209 | 114–148 | 123–135 | ? | 89–157 | 83–118 | 154–195 | 127–142 | ? | 81* |
| Neck region | 168–208 | 215* | 202–236* | 194–239* | ? | 178–250* | ? | ? | 177* | 137* | 157–332* | 146–180 | 139–150 | 153–166* | 120–177 | 134–153 | 241–327* | 149–172 | ? | ? |
| Midbody diam. | 70–112 | 56.6* | ? | 59–113 | ? | 52–170 | ? | ? | 40* | 39* | 49–73 | 33–40 | 30–34 | 18–21 | ? | 13–17 | 49–72 | 35–41 | ? | 29.4* |
| Vulva – anterior end | 749–1046 | 680–840 | 573* | 637* | 503–550 | 616–1225 | 503–550 | ? | 650* | 503–550 | 1001* | 447–629 | 436–491 | 495.2* | ? | ? | 875* | ? | 840 | 291.6* |
| Body diam. at anus | 21–39 | 29* | 19* | 28–36 | ? | 25–50 | ? | ? | 30* | 18* | 22–31 | 18–22 | 19–20 | 19–21 | ? | 20–23 | 25* | 21–25 | 34 | 15* |
| Rectum length | 12–43 | 37.5* | ? | 31–42 | ? | 24–30 | ? | ? | ? | 19* | 27–42 | 22–25 | 21–29 | 17.2* | ? | ? | 23–32 | 17.8 | ? | ? |
| Tail length | 41–62 | 39–50 | 39–50 | 10–15 | 35–45 | 42–55 | 35–45 | ? | 50* | 39–56 | 41–60 | 36–45 | 35–40 | 37–42 | 61–111 | 30–37 | 41–52 | 88–91 | 66 | 39–43 |
| Phasmid to anus distance | 21–39 | 10–19 | 23.3* | ? | ? | ? | ? | ? | ? | 18.8* | 22–36 | 16–29 | 17.5* | 20.7* | 41–66 | ? | 21–32 | 56–64 | ? | ? |
| D% (EP/ES×100) | 64–101 | 74–86* | 74–86* | 82* | 79* | 79* | ? | ? | 88* | 80.5* | 64–66 | 87* | 94* | ? | ? | ? | 68* | 88* | ? | ? |
| E% (EP/T×100) | 206–320 | 359–376* | 359–376* | ? | ? | 285* | ? | ? | 278* | 265.3* | 312–348 | 323* | 344* | ? | ? | ? | 378* | 150* | ? | ? |
Pairwise distances of the 18 S rDNA regions between present strains of Acrobeloides and already described species_
| S. No. | 18S rDNA | Country | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | MK935150 A. saeedi KMW | India | 0 | 0 | 9 | 19 | 19 | 19 | 19 | 24 | 30 | 89 | |
| 2 | MK935149 A. saeedi DH1 | India | 100 | 0 | 9 | 19 | 19 | 19 | 19 | 24 | 30 | 89 | |
| 3 | KY090631 A. saeedi | Iran | 100 | 100 | 9 | 19 | 19 | 19 | 19 | 31 | 23 | 89 | |
| 4 | MK541681 A. tricornis | Germany | 98.5 | 98.5 | 98.3 | 0 | 0 | 0 | 0 | 10 | 10 | 9 | |
| 5 | DQ102707 A. nanus | UK | 98.5 | 98.5 | 98.5 | 100 | 16 | 2 | 0 | 39 | 13 | 97 | |
| 6 | KX889085 A. varius | South Korea | 98.5 | 98.5 | 98.5 | 100 | 99.3 | 2 | 0 | 39 | 13 | 96 | |
| 7 | AY284673 A. apiculatus | Netherlands | 98.5 | 98.5 | 98.5 | 100 | 99.9 | 99.9 | 0 | 37 | 13 | 95 | |
| 8 | MF325099 A. buchneri | Germany | 98.4 | 98.4 | 98.5 | 100 | 100 | 100 | 100 | 19 | 4 | 86 | |
| 9 | AF202159 A. bodenheimeri | France | 98.1 | 98.1 | 97.4 | 98.6 | 98.3 | 98.3 | 98.4 | 98.4 | 32 | 92 | |
| 10 | KY119635 A. thornei | Ireland | 97.5 | 97.5 | 98.1 | 98.2 | 99.0 | 99.0 | 99.0 | 99.7 | 97.4 | 95 | |
| 11 | JQ237850 A. maximus | USA | 92.3 | 92.3 | 91.7 | 98.8 | 95.0 | 95.1 | 95.1 | 92.0 | 95.3 | 91.3 |