Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Mgonja, Doreen M.; Temu, Gladness E.; Ndunguru, Joseph C.; Mziray, Magreth F.; Lyantagaye, Sylvester L.; Luambano, Nessie D.

Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Volume 52 (2020): Issue 1 (January 2020)

By:

Doreen M. Mgonja, Gladness E. Temu, Joseph C. Ndunguru, Magreth F. Mziray, Sylvester L. Lyantagaye and Nessie D. Luambano

Open Access

|Mar 2020

Figures & Tables

Micrographs of Radophilis similis collected from Tanzania. Representative anatomy shown for males (A-F) and females (G-L): (A, G) Entire body structure; (B, H) pharyngeal region; (C, I) posterior region; (D, J) anterior region (stylet, stylet knobs, and median bulb shown by red, yellow, and purple arrows, respectively); (E, K) posterior region (vulva and spicule shown by white arrows); and (F, L) tail region. Scale bars: 50 µm (A-G) and 25 µm (B-F, H-L).

Bayesian consensus trees inferred for Radophilis similis. (A) Tree inferred from SSU & ITS1 rRNA. (B) Tree inferred from ITS1 and ITS2 rRNA. (C) Tree inferred from LSU rRNA. Posterior probability values >50% are shown. Sequences original to this study are labeled green, red, and blue for trees in (A), (B), and (C) respectively; sequences labeled pink represent outgroup species.

Morphometrics (µm) of adult R_ similis female and male populations collected from the agro-ecological zones sampled_ Data are means ± SD and standard range (Roy et al_, 2018; Elbadri et al_, 1999) in parentheses_

	Female populations				Male populations
Parameter^a	Lake zone	Northern zone	Southern Highlands zone	Zanzibar islands	Lake zone	Northern zone	Southern Highlands zone	Zanzibar islands
L	520.5 ± 11.4	564.1 ± 11.4	554.3 ± 11.4	581.0 ± 11.4	486.3 ± 12.2	566.1 ± 12.2	542.8 ± 12.2	570.5 ± 12.2
	(500 – 600)	(500–600)	(500 – 600)	(500 – 600)	(440 – 685)	(440–685)	(440 – 685)	(440 – 685)
a	28.5 ± 5.1	28.5 ± 5.1	27.3 ± 3.9	30.3 ± 2.7	29.5 ± 3.8	31.8 ± 5.2	33.1 ± 3.6	33.1 ± 3.6
	(20 – 34.0)	(20 – 34.0)	(20 – 34.0)	(20 – 34.0)	(24.5 – 42.5)	(24.5 – 42.5)	(24.5 – 42.5)	(24.5 – 42.5)
b	6.3 ± 1.1	4.93 ± 0.7	5.79 ± 0.7	5.6 ± 0.8	5.2 ± 0.3	5.3 ± 0.5	5.4 ± 0.3	5.1 ± 0.2
	(4.2 – 6.6)	(4.2 – 6.6)	(4.2 – 6.6)	(4.2 – 6.6)	(4.1 – 5.6)	(4.1 – 5.6)	(4.1 – 5.6)	(4.1 – 5.6)
b'	5.1 ± 0.5	4.2 ± 0.6	4.9 ± 0.5	4.8 ± 0.6	5.4 ± 0.5	5.4 ± 0.7	5.5 ± 0.8	5.9 ± 0.7
	(3.3 – 5.7)	(3.3 – 5.7)	(3.3 – 5.7)	(3.3 – 5.7)	(3.7 – 6.8)	(3.7 – 6.8)	(3.7 – 6.8)	(3.7 – 6.8)
c	8.2 ± 1.0	7.9 ± 0.5	7.5 ± 0.5	8.4 ± 1.2	6.8 ± 0.3	6.8 ± 0.6	7.3 ± 0.1	6.9 ± 0.6
	(6.8 – 12.2)	(6.8 – 12.2)	(6.8 – 12.2)	(6.8 – 12.2)	(6.3 – 10.3)	(6.3 – 10.3)	(6.3 – 10.3)	(6.3 – 10.3)
c'	4.4 ± 0.7	4.2 ± 1.0	5.12 ± 0.8	4.8 ± 0.5	5.8 ± 0.7	5.7 ± 1.0	5.8 ± 1.1	6.4 ± 0.7
	(2.8 – 6.2)	(2.8 – 6.2)	(2.8 – 6.2)	(2.8 – 6.2)	(3.7 – 6.9)	(3.7 – 6.9)	(3.7 – 6.9)	(3.7 – 6.9)
Sl	10.8 ± 1.1	10.6 ± 1.1	10.7 ± 1.1	10.4 ± 1.1	10.3 ± 2.2	10.0 ± 2.0	9.8 ± 2.2	8.7 ± 2.2
	(14 – 18)	(14 – 18)	(14 – 18)	(14 – 18)	(8 – 13)	(8 – 13)	(8 – 13)	(8 – 13)
V	51 ± 0.9	55.5 ± 0.9	55.4 ± 0.8	58.2 ± 0.7	131.7 ± 2.1	144.0 ± 2.1	131.6 ± 2.2	131.7 ± 2.1
	(50.7 – 59)	(50.7 – 59)	(50.7 – 59)	(50.7 – 59)	(128 – 250)	(128 – 250)	(128 – 250)	(128 – 250)
W	16.0 ± 3.0	18.0 ± 3.0	17.6 ± 3.0	18.8 ± 3.0	14.5 ± 2.0	16.1 ± 2.1	14.7 ± 2.1	14. 7 ± 2.3
	(13 – 21)	(13 – 21)	(13 – 21)	(13 – 21)	(14 – 18)	(14 – 18)	(14 – 18)	(14 – 18)
t	64.7 ± 2.0	73.3 ± 2.3	75.2 ± 2.0	72.3 ± 2.6	69.2 ± 2.0	81.8 ± 1.9	72.0 ± 2.1	80.5 ± 2.0
	(52 – 100)	(52 – 100)	(52 – 100)	(52 – 100)	(60 – 90)	(60 – 90)	(60 – 90)	(60 – 90)
OL	72.5 ± 2.6	91.8 ± 2.6	82.7 ± 3.1	84.8 ± 3.6	63.8 ± 2.1	80.1 ± 2.2	76.4 ± 2.2	72.5 ± 2.0
	(69 – 99)	(69 – 99)	(69 – 99)	(69 – 99)	(62 – 123)	(62 – 123)	(62 – 123)	(62 – 123)
DGO	3.0 ± 0.4	3.5 ± 0.5	3.2 ± 0.5	2.6 ± 0.5	1.8 ± 0.1	2.4 ± 0.5	2.6 ± 0.3	1.5 ± 0.3
	(2.0 – 5.5)	(2.0 – 5.5)	(2.0 – 5.5)	(2.0 – 5.5)	(1.5–3.0)	(1.5 – 3.0)	(1.5 – 3.0)	(1.5 – 3.0)

DOI: https://doi.org/10.21307/jofnem-2020-020 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 1 - 8

Submitted on: Aug 2, 2019

Published on: Mar 17, 2020

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Banana,

Burrowing nematodes,

Genetic variation,

spp.,

Pest management,

Plant-parasitic nematodes,

Taxonomy.

Related subjects:

Life sciences,

Life sciences, other

© 2020 Doreen M. Mgonja, Gladness E. Temu, Joseph C. Ndunguru, Magreth F. Mziray, Sylvester L. Lyantagaye, Nessie D. Luambano, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Figures & Tables

Figure 1:

Figure 2:

Morphometrics (µm) of adult R_ similis female and male populations collected from the agro-ecological zones sampled_ Data are means ± SD and standard range (Roy et al_, 2018; Elbadri et al_, 1999) in parentheses_

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