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Description of Laimaphelenchus sinensis n. sp. (Nematoda: Aphelenchoididae) from declining Chinese pine, Pinus tabuliformis in Beijing, China

Open Access
|Mar 2020

Figures & Tables

Figure 1:

Line drawings of Laimaphelenchus sinensis n. sp. A: Entire female; B: Entire male; C: Anterior region; D: Female posterior region showing vulva and post-uterine sac; E: Lateral lines F, G: Female tail terminus; H: Male tail; I: Spicule. (Scale bars = A, B = 20 μm; C-I = 10 μm).
Line drawings of Laimaphelenchus sinensis n. sp. A: Entire female; B: Entire male; C: Anterior region; D: Female posterior region showing vulva and post-uterine sac; E: Lateral lines F, G: Female tail terminus; H: Male tail; I: Spicule. (Scale bars = A, B = 20 μm; C-I = 10 μm).

Figure 2:

Light photomicrographs of Laimaphelenchus sinensis n. sp. A: Entire female; B: Entire male; C: Lateral lines; D: Anterior region; E: Female posterior region showing vulva and post-uterine sac; F, G: Vulval regions; H: Female tail; I-K: Female tail terminus; L-N: Male tails arrows showing position of caudal papillae (Scale bars = A, B = 20 μm; C-N = 10 μm; Abbreviations: ex, excretory pore).
Light photomicrographs of Laimaphelenchus sinensis n. sp. A: Entire female; B: Entire male; C: Lateral lines; D: Anterior region; E: Female posterior region showing vulva and post-uterine sac; F, G: Vulval regions; H: Female tail; I-K: Female tail terminus; L-N: Male tails arrows showing position of caudal papillae (Scale bars = A, B = 20 μm; C-N = 10 μm; Abbreviations: ex, excretory pore).

Figure 3:

Phylogenetic relationships of Laimaphelenchus sinensis n. sp. and aphelenchid nematodes based on full length of 18 S rDNA. The 100001st Bayesian tree inferred from 18 S rDNA under TVM + I + G model. Aphelenchus avenae (JQ348399) served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.
Phylogenetic relationships of Laimaphelenchus sinensis n. sp. and aphelenchid nematodes based on full length of 18 S rDNA. The 100001st Bayesian tree inferred from 18 S rDNA under TVM + I + G model. Aphelenchus avenae (JQ348399) served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

Figure 4:

Phylogenetic relationships of Laimaphelenchus sinensis n. sp. and aphelenchid nematodes based on D2-D3 expansion segments of 28 S rDNA. The 100001st Bayesian tree inferred from 28 S rDNA under TIM2 + I + G model. Aphelenchus avenae (JQ348400) served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.
Phylogenetic relationships of Laimaphelenchus sinensis n. sp. and aphelenchid nematodes based on D2-D3 expansion segments of 28 S rDNA. The 100001st Bayesian tree inferred from 28 S rDNA under TIM2 + I + G model. Aphelenchus avenae (JQ348400) served as the outgroup species. Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics data for Laimaphelenchus sinensis n_ sp_

FemaleMale
CharacterHolotypeParatypesParatypes
n65
L914968±46.1 (914-1064)876±69.1 (750-956)
a42.141.5±1.9 (38.6-44.8)46.2±2.6 (42.4-50)
b11.211.7±0.6 (11.2-12.7)10.5±0.6 (9.4-11.1)
b’4.34.8±0.4 (4.3-5.6)4.6±0.3 (4.1-4.9)
c25.328±3.1 (25-32.9)19.9±1.4 (17.9-22.3)
c’2.62.6±0.3 (2.1-2.9)2.7±0.2 (2.4-2.9)
V or T70.569.1±0.9 (67.7-70.7)70.8±4.4 (63.4-76.5)
Lip region height2.32.4±0.1 (2.2-2.6)2.7±0.1 (2.6-2.8)
Lip region width6.97.1±0.3 (6.8-7.4)7.1±0.6 (6.4-7.9)
Stylet length12.212.3±0.3 (11.8-12.6)12.3±0.6 (11.1-12.9)
Body diam.21.723.4±1.7 (21.6-26.4)19±1.1 (17.7-20.9)
Median bulb width18.913.2±0.7 (12.4-14)12±0.7 (11-12.8)
Median bulb length12.618.5±0.5 (17.6-18.9)17.4±0.8 (16.2-18)
Median bulb length/diam. ratio1.51.4±0.1 (1.3-1.5)1.5±0.1 (1.4-1.5)
Excretory pore from anterior end92.296.3±4.7 (88-104)83.2±2.5 (80.1-87)
Ovary length or testis430454±24 (420-480)622.6±76.6 (476-690)
Post-uterine sac121130.3±6.1 (119-138)
Vulva to anus distance270264.2±20.9 (233-300)
Post-uterine sac length/vulva to anus (%)44.849.8±5.5 (39.7-55.8)
Anal (cloacal) body diameter14.113.3±0.7 (12-14.1)16.3±1.0 (15.3-18.2)
Tail length36.134.9±3.2 (29-38.6)44.1±1.8 (41.8-46.4)
Spicule (curved median line)14.0±0.6 (13.2-15)
Spicule (Chord)15.6±0.9 (14.1-16.6)
DOI: https://doi.org/10.21307/jofnem-2020-019 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 9
Submitted on: Sep 8, 2019
Published on: Mar 17, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Jianfeng Gu, Munawar Maria, Yiwu Fang, Lele Liu, Yong Bian, Xianfeng Chen, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.