Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet

Quick, Richard A.; Cimrhakl, Launa; Mojtahedi, Hassan; Sathuvalli, Vidyasagar; Feldman, Maximilian J.; Brown, Charles R.

Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet

Journal of Nematology

Volume 52 (2020): Issue 1 (January 2020)

By:

Richard A. Quick, Launa Cimrhakl, Hassan Mojtahedi, Vidyasagar Sathuvalli, Maximilian J. Feldman and Charles R. Brown

Open Access

|Mar 2020

Figures & Tables

Two weeks post-transplant, plant hosts were inoculated to evaluate their ability to host Stubby root nematodes (SRN) and Tobacco rattle virus (TRV). At month zero, test hosts were transplanted into pots containing viruliferous SRNs and cultured for one to three months. Each month a subset of hosts is sacrificed, nematode counts are taken, and a tobacco bait plant is transplanted into the test pot. After one month the tobacco bait plant is harvested and both foliar and root tissue was tested for the presence of TRV using RT-PCR.

Population counts of stubby root nematode, Paratrichodorus allius, across three months in pots grown with alfalfa, Russet Burbank potato, Castle Russet potato and tobacco; inoculated with 60 and 1,060 nematodes/10 L soil in Experiment 1 and 2, respectively.

Number of bait plant tissue samples that tested positive and negative for the presence of TRV by RT-PCR. (A) Foliage tissue in Experiment 1 (B) Root tissue in Experiment 1 (C) Foliage tissue in Experiment 2 (D) Root tissue in Experiment 2.

Nematode reproduction factor (Rf) estimates calculated from 250 cm3 soil samples collected from four plant genotypes, across three months, in two independent experiments. Individual data points are plotted as circular dots, whereas the population mean is plotted using an “X” symbol and standard error of the mean is denoted by the error bars. Experiments are differentiated by inoculation pressure. (A) Experiment 1 (60 nematodes/10 L) is plotted in orange (B) Experiment 2 (1,060 nematodes/10 L) plotted in red.

Average nematode count, reproduction factor (Rf) values and proportion of foliage and root tissue samples with TRV infection at the final sampling time point_

Plant	Experiment	Nematode count	Rf value	Foliage TRV infection (%)	Root TRV infection (%)
Alfalfa	1	126.40^b	84.26^b	0^a,b	0^b
Burbank	1	20.00^d	13.33^b	33^a,b	50^a,b
Castle	1	119.87^c	79.91^b	0^b	0^b
Tobacco	1	720.80^a	480.53^a	80^a	100^a
Alfalfa	2	6.00^d	0.22^c	0^b	20^a
Burbank	2	64^b	2.41^b	100^a	100^a
Castle	2	19.6^c	0.73^b,c	0^b	0^a
Tobacco	2	768^a	28.98^a	100^a	80^a

DOI: https://doi.org/10.21307/jofnem-2020-011 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 1 - 10

Submitted on: Aug 12, 2019

Published on: Mar 20, 2020

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Potato,

Related subjects:

© 2020 Richard A. Quick, Launa Cimrhakl, Hassan Mojtahedi, Vidyasagar Sathuvalli, Maximilian J. Feldman, Charles R. Brown, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet

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Average nematode count, reproduction factor (Rf) values and proportion of foliage and root tissue samples with TRV infection at the final sampling time point_

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