Figure 1:
The morphological variations within the P. vulnus Taiwan strawberry population. Two lateral field types and four tail termini were observed. Photographs were taken at 1000X magnification with compound microscope and SEM.
Table 1.
GenBank sequence deposit information of multiple gene regions of P. vulnus Taiwan isolates from strawberry fields GS and KDL.
| P. vulnus sequences GenBank deposit information | ||||
|---|---|---|---|---|
| rDNA | mtDNA | |||
| Isolate location | LSU (730 bp) | ITS (669 bp) | SSU (883 bp) | COI (441 bp) |
| GS | MG372808 | MG372806.1 | MG372807 | MN431203 |
| KDL | MK713641 | MK713613 | MK713614 | MK764689 |
Table 2.
Measurements of the Taiwan P. vulnus population morphological characteristics.
| Female (n = 30) | Male (n = 30) | |||||
|---|---|---|---|---|---|---|
| Characteristics | Mean | SD m. | Range | Mean | SD m. | Range |
| L (μm) | 704.01 | 74.85 | 573.46–839.91 | 587.02 | 46.83 | 492.76–678.1 |
| K (μm) | 25.97 | 4.06 | 17.76–36.18 | 18.47 | 1.47 | 15.49–21.50 |
| Stylet (μm) | 14.5 | 1.1 | 12.6–16.5 | 13.76 | 0.73 | 12.18–15.40 |
| Tail (μm) | 32.87 | 3.93 | 24.35–41.10 | 28.74 | 4.07 | 22.28–39.80 |
| a (ratio) | 27.47 | 3.15 | 22.74–34.30 | 31.85 | 2.53 | 26.83–36.62 |
| b (ratio) | 6.1 | 0.6 | 4.87–7.16 | 5.7 | 0.47 | 4.88–6.95 |
| b’ (ratio) | 4.86 | 0.42 | 4.07–5.59 | 4.51 | 0.37 | 3.92–5.26 |
| c (ratio) | 21.57 | 2.38 | 17.83–27.46 | 20.71 | 2.68 | 13.51–23.99 |
| c’ (ratio) | 2.29 | 0.31 | 1.59–2.86 | 2.29 | 0.38 | 1.77–3.83 |
| V (%) | 78.34 | 1.67 | 73.84–81.09 | – | – | – |
| Spicules (μm) | – | – | – | 16.88 | 1.89 | 12.06–20.15 |
| DGO (μm) | 3.55 | 0.32 | 3.00–4.42 | 3.41 | 0.33 | 2.77–4.31 |
Figure 2:
The phylogenetic tree of the combined partial 28S D2-D3 region and complete ITS region of the P. vulnus rDNA sequences. Morphologically similar species, Pratylenchus penetrans F1 isolate and of P. kumamotoensis Chilgok isolate, were used as out groups. Each node is marked with the isolate code, species and origin. The number at the fork represents the percentage of the bootstrap tested with 10,000 times for the indicating result.