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Active and inactive forms of biotin synthase occur in Heterodera glycines

Journal of Nematology
Volume 51 (2019): Issue 1 (January 2019)
By:
Open Access
|Oct 2019

Figures & Tables

Figure 1

Complementation by expression of HgBioB in biotin synthase-deficient E. coli strain. (A) HgBioB construct diagrams showing polymorphic amino acids, from N-terminus to C-terminus, at positions 24 and 44. HgBioB-vir was mutagenized to produce HgBioB-vir-M1, by generating an alanine to proline mutation at position 24 (A24P); and HgBioB-vir-M2, by generating a glutamine to arginine mutation at position 44 (Q44R). (B) The expression of the vector control, HgBioB-avr, HgBioB-vir, and two mutagenized forms of the virulent allele HgBioB-vir-M1 and HgBioB-vir-M2 on minimal media with and without supplemental biotin.
Complementation by expression of HgBioB in biotin synthase-deficient E. coli strain. (A) HgBioB construct diagrams showing polymorphic amino acids, from N-terminus to C-terminus, at positions 24 and 44. HgBioB-vir was mutagenized to produce HgBioB-vir-M1, by generating an alanine to proline mutation at position 24 (A24P); and HgBioB-vir-M2, by generating a glutamine to arginine mutation at position 44 (Q44R). (B) The expression of the vector control, HgBioB-avr, HgBioB-vir, and two mutagenized forms of the virulent allele HgBioB-vir-M1 and HgBioB-vir-M2 on minimal media with and without supplemental biotin.

Figure 2

Box plots of HgBioB amino acid polymorphism trends among SCN field populations, showing the percentage of amino acid differences for A24P (A) and Q44R (B).
Box plots of HgBioB amino acid polymorphism trends among SCN field populations, showing the percentage of amino acid differences for A24P (A) and Q44R (B).

Figure 3

Scatterplots showing the HgBioB avirulent and virulent polymorphic amino acid pairs. (A) The avirulent pair shows a positive linear relationship between amino acid positions P24 and R44. (B) Similarly, the virulent pair also shows a positive linear relationship between amino acid positions A24 and Q44.
Scatterplots showing the HgBioB avirulent and virulent polymorphic amino acid pairs. (A) The avirulent pair shows a positive linear relationship between amino acid positions P24 and R44. (B) Similarly, the virulent pair also shows a positive linear relationship between amino acid positions A24 and Q44.
DOI: https://doi.org/10.21307/jofnem-2019-069 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 12
Submitted on: Apr 29, 2019
Published on: Oct 25, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Biochemistry,
Biotin,
Biotin synthase,
Dethiobiotin,
Horizontal gene transfer,
Soybean cyst nematode,
Vitamin B
Related subjects:
Life sciences,
Life sciences, other

© 2019 Khee Man Kwon, Sadia Bekal, Leslie L. Domier, Kris N. Lambert, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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