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Identification of Suitable Meloidogyne spp. Housekeeping Genes Cover

Identification of Suitable Meloidogyne spp. Housekeeping Genes

Journal of Nematology
Volume 51 (2019): Issue 1 (January 2019)
By: Weiming Hu and  Peter M. DiGennaro  
Open Access
|Sep 2019
Figures & tables

Figures & Tables

Figure 1:

Candidate housekeeping gene selection pipeline.

Figure 2:

Summary of KEGG annotation of the candidate housekeeping genes.

Figure 3:

Stability value of validated housekeeping genes across life cycle of M. incognita calculated by geNorm. Juvenile stage samples were collected within the first week, adult stage samples were collected 3 wk after inoculation, and all stages samples include previous stages, eggs and uninfected juveniles. Orange bars are negative control which were differently expressed genes (Colla: DEG = 10; NHL: DEG = 1), and blue bars are candidate housekeeping genes. Genes with stability value below 0.15 are considered suitable reference genes.

Figure 4:

Stability value of validated housekeeping genes across life cycle of M. incognita calculated by NormFinder. Juvenile stage samples were collected within the first week, adult stage samples were collected 3 wk after inoculation, and all stages samples include previous stages, eggs and uninfected juveniles. Orange bars are negative control which were differently expressed genes (Colla: DEG = 10; NHL: DEG = 1), and blue bars are candidate housekeeping genes. Genes with stability value below 0.08 are considered suitable reference genes for this study.
DOI: https://doi.org/10.21307/jofnem-2019-055 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 11
Submitted on: Apr 11, 2019
Published on: Sep 20, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
housekeeping gene,
Root-knot nematode,
RT-qPCR,
method,
molecular biology,
genetics.
Related subjects:
Life sciences,
Life sciences, other

© 2019 Weiming Hu, Peter M. DiGennaro, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 51 (2019): Issue 1 (January 2019)
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