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Identification of Suitable Meloidogyne spp. Housekeeping Genes

Journal of Nematology
Volume 51 (2019): Issue 1 (January 2019)
By:
Open Access
|Sep 2019

Figures & Tables

Figure 1:

Candidate housekeeping gene selection pipeline.
Candidate housekeeping gene selection pipeline.

Figure 2:

Summary of KEGG annotation of the candidate housekeeping genes.
Summary of KEGG annotation of the candidate housekeeping genes.

Figure 3:

Stability value of validated housekeeping genes across life cycle of M. incognita calculated by geNorm. Juvenile stage samples were collected within the first week, adult stage samples were collected 3 wk after inoculation, and all stages samples include previous stages, eggs and uninfected juveniles. Orange bars are negative control which were differently expressed genes (Colla: DEG = 10; NHL: DEG = 1), and blue bars are candidate housekeeping genes. Genes with stability value below 0.15 are considered suitable reference genes.
Stability value of validated housekeeping genes across life cycle of M. incognita calculated by geNorm. Juvenile stage samples were collected within the first week, adult stage samples were collected 3 wk after inoculation, and all stages samples include previous stages, eggs and uninfected juveniles. Orange bars are negative control which were differently expressed genes (Colla: DEG = 10; NHL: DEG = 1), and blue bars are candidate housekeeping genes. Genes with stability value below 0.15 are considered suitable reference genes.

Figure 4:

Stability value of validated housekeeping genes across life cycle of M. incognita calculated by NormFinder. Juvenile stage samples were collected within the first week, adult stage samples were collected 3 wk after inoculation, and all stages samples include previous stages, eggs and uninfected juveniles. Orange bars are negative control which were differently expressed genes (Colla: DEG = 10; NHL: DEG = 1), and blue bars are candidate housekeeping genes. Genes with stability value below 0.08 are considered suitable reference genes for this study.
Stability value of validated housekeeping genes across life cycle of M. incognita calculated by NormFinder. Juvenile stage samples were collected within the first week, adult stage samples were collected 3 wk after inoculation, and all stages samples include previous stages, eggs and uninfected juveniles. Orange bars are negative control which were differently expressed genes (Colla: DEG = 10; NHL: DEG = 1), and blue bars are candidate housekeeping genes. Genes with stability value below 0.08 are considered suitable reference genes for this study.
DOI: https://doi.org/10.21307/jofnem-2019-055 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 11
Submitted on: Apr 11, 2019
Published on: Sep 20, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
housekeeping gene,
Root-knot nematode,
RT-qPCR,
method,
molecular biology,
genetics.
Related subjects:
Life sciences,
Life sciences, other

© 2019 Weiming Hu, Peter M. DiGennaro, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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