Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)

Nguyen, Huu Tien; Trinh, Quang Phap; Couvreur, Marjolein; Singh, Phougeishangbam Rolish; Decraemer, Wilfrida; Bert, Wim

Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)

Journal of Nematology

Volume 51 (2019): Issue 1 (January 2019)

By:

Huu Tien Nguyen, Quang Phap Trinh, Marjolein Couvreur, Phougeishangbam Rolish Singh, Wilfrida Decraemer and Wim Bert

Open Access

|Apr 2019

Figures & Tables

Rotylenchus rhomboids n. sp., (drawings ♀). (A) entire body; (B) en face view; (C) head region; (D) esophageal region; (E) lateral field at vulva region; (F) vulva region; (G) tail region; (H) lateral field at tail region. (B, C based on SEM illustrations) (A, E, F, G, H: Holotype) (Scale bar: A: 25 µm; B: 5 µm; C: 20 µm; D–H: 25 µm).

Rotylenchus rhomboides n. sp. (LM ♀). (A) esophageal region; (D) vulva region; (E) lateral field at vulva region with a rhomboid widening (see arrow); (G) entire body; (H) tail region; (I) lateral field with phasmid at tail region (SEM ♀); (B) anterior body region, lateral view; (C) en face view (arrow indicates amphid aperture); (F) vulva region, lateral view; (J) vulva region, ventral view; (K) tail region of female, lateral view; (L) tail region of female, oblique ventral view; (M) tail region of juveniles (D, E, G, H, I: Holotype; others paratypes) (Scale bar: B, C: 1 µm; A, D–M: 25 µm).

BI phylogenetic tree generated from the D2-D3 of 28S rDNA region data set with the HKY + G model (94 parameters). Bayesian posterior probabilities are given next to each node. Sequences of Rotylenchus rhomboides n. sp. and R. buxophilus (Belgium) are in bold.

BI phylogenetic tree generated from ITS sequences with HKY + G model (70 parameters). Bayesian posterior probabilities are given next to each node. Sequences of Rotylenchus rhomboides n. sp. and R. buxophilus (Belgium) are in bold.

BI phylogenetic tree generated from COI sequences with the GTR +G + I model (77 parameters). Bayesian posterior probabilities are given next to each node. Sequences of Rotylenchus rhomboides n. sp. and R. buxophilus (Belgium) are in bold.

The cluster analysis of 104 species (included Rotylenchus n. sp.) based on Bray–Curtis similarity measure of 11 ranked features. Rotylenchus rhomboides n. sp. is in bold. The dotted line indicates 85% similarity.

Belgian population of Rotylenchus buxophilus (LM ♀). (A) entire body; (B) esophageal region; (C) lateral field at vulva region; (D) vulva region; (E) lateral field with phasmid at tail region; (F) tail region (Scale bar: A–F: 25 µm).

Morphometric data of Rotylenchus buxophilus from Belgium_ All measurements are in μm (except for ratio) and in the form: mean ± sd (range)_

Character	Females
n	15
Body length (L)	972 ± 87 (818–1065)
a = L/MBD	30.1 ± 1.9 (27.2–32.5)
b″ = L/Anterior end to the end of esophageal gland	7.0 ± 0.8 (5.3–8.2)
c = L/Tail length	40.1 ± 4.1 (34.6–49.8)
c″ = Tail length/ABD	1.2 ± 0.1 (1.0–1.4)
V = Anterior end to vulva/L × 100	53 ± 3 (48–58)
Lip height	7 ± 0.3 (6–8)
Lip width	11 ± 0.6 (10–12)
Stylet length	36 ± 1.6 (32–38)
Conus length	18 ± 1.1 (16–20)
Shaft length	14 ± 0.7 (13–14)
Knob height	4 ± 0.6 (3–5)
Dorsal gland opening from stylet base	4 ± 1.2 (3–7)
Anterior end to secretory-excretory pore	123 ± 10 (105–139)
Anterior end to nerve ring	99 ± 7 (87–109)
Anterior end to esophageal gland end	140 ± 11 (124–157)
Esophagus overlapping	22 ± 5 (18–28)
Max body diameter (MBD)	32 ± 2 (29–37)
Anal body diameter (ABD)	21 ± 1.6 (19–24)
Tail length	24 ± 3.1 (18–28)
Tail annuli number	14 ± 1.3 (12–16)

Comparison of the matrix code of Rotylenchus rhomboides n_ sp_ with other closely related species_

Species	A	B	C	D	E	F	G	H	I	J	K
Rotylenchus rhomboides n. sp.	3–4	2	1	2	2	2	2	1–2	2	2	2
R. buxophilus from Belgium	4	1	1	4	2	2	3	3	2	2	1
R. buxophilus	4	1	1	4	2	2	3	3	2	2	1
R. corsicus	4	1	1	2	2	2	2	3	2	2	1
R. goodeyi	3	1	1	4	2	2	4	2	2	1	2
R. gracilidens	3	1	1	2	2	2	3	1	2	1	1
R. incultus	4	1	1	4	1	2	3	1	2	1	1
R. laurentinus	4	1	1	3	2	2	3	1	2	1	2
R. pumilus	4	1	1	4	1	2	2	1	2	1	2
R. rugatocuticulatus	3	2	1	2	3	2	4	1	2	1	1

The measurements of known and new populations of Rotylenchus buxophilus_

Female	R. buxophilus (Belgian population)	R. buxophilus Golden (1956)	R. buxophilus Sher (1965)	R. buxophilus = R. sheri Jairajpuri (1963)	R. buxophilus Geraert and Barooti (1996)	R. buxophilus Wouts and Sturhan (1999)
Host, location	Yam (Dioscorea tokoro), Belgium	English boxwood (Buxus sempervirens var. suffruticosa L.), USA	camellia (Camellia japonica L.), France	Cedrus libani var. deodara, India	Magnolia sp. L., Iran	Cabbage tree (Cordyline sp. Comm, ex R.Br.), New Zealand
n	15	20	20	10	4	6
L	972 (818–1065)	1,090	920–1,310	900–1,200	850–1,070	1,020–1,250
V	53 (48–58)	55	52–58	52–56	54–58	55–58
STL	36 (32–38)	33.5	34–38	35–39	34	38–40
a	30.1 (27.2–32.5)	31	28–38	27–35	25–32	32–43
b	7.0 (5.3–8.2)	7	6.4–8.9	5.5–7.0	6–8	7.3–8.7
c	40.1 (34.6–49.8)	43	36–48	38–47	33–45	41–58
c′	1.2 (1.0–1.4)	–	–	–	1.1–1.4	0.8–1.2

Rotylenchus species and populations used and sequenced in the present study_

	Accession number
Species	28S	ITS	COI	Host	Locality
H. dihystera	AB933471			Sugarcane	Japan
H. multicinctus	MF401446			Banana	Greece
H. columbus		FJ485623		Unknown	USA
H. seinhorsti		KX446971		Unknown	China
R. arasbaranensis	KJ765335	KJ765345		Grasses	Iran
	KJ765336	KJ765346		Grasses	Iran
R. brevicaudatus	JX015419			Grasses	Australia
	JX015420			Grasses	Australia
		JX015431		Grasses	Australia
R. buxophilus	JX015421			–	USA
	KJ765337			Euonymus sp.	Iran
	KJ765338			Euonymus sp.	Iran
	KJ765340			Euonymus sp.	Iran
	KU722390			–	Iran
		JX015432		Unknown	USA
		JX015433		Unknown	USA
		KJ765347		Euonymus sp.	Iran
R. buxophilus	MK114140	MK114142	MK114155	Dioscorea tokoro	Belgium
	MK114141	MK114143	MK114156	Dioscorea tokoro	Belgium
R. castilloi	KM251415			Fagus orientalis Lipsky (oriental beech tree)	Iran
R. cazorlaensis			JX015399	Quercus faginea (Portuguese oak)	Spain
			JX015400	Quercus faginea (Portuguese oak)	Spain
R. conicaudatus	HQ700698			Grasses	Iran
R. cretensis	KT288060			Olive	Greece
R. cypriensis	KP843116			Prunus sp.	Iran
	KT288062			Olive	Greece
R. dalikhaniensis	KM042108			Ruscus hyrcanus Woronow	Iran
R. eximius	KX398058			Tamarix smyrnensis	Greece
	KX669231		KX669244	Olive	Tunisia
	KX669232			Olive	Tunisia
	KX669233		KX669245	Olive	Tunisia
			JX015401	Pistacia lentiscus (lentisc)	Italy
			JX015402	Pistacia lentiscus (lentisc)	Italy
R. goodeyi	DQ328756			Olea europaea subsp. silvestris (wild olive)	Spain
R. incultus	KX669234			Olive	Tunisia
	KX669235			Olive	Tunisia
	KX669236			Olive	Tunisia
			JX015403	Vitis vinifera (grapevine)	Spain
			KX669246	Olive	Tunisia
			KX669247	Olive	Tunisia
			KX669248	Olive	Tunisia
			KX669249	Olive	Tunisia
R. iranicus	HQ700697			Unidentified forest tree	Iran
R. jaeni			JX015404	Quercus suber (cork tree)	Spain
R. laurentinus	DQ328757		JX015405	Pistacia lentiscus (lentisc)	Italy
			JX015406	Pistacia lentiscus (lentisc)	Italy
			JX015407	Pistacia lentiscus (lentisc)	Italy
	EU280798			Pistacia lentiscus (lentisc)	Spain
R. magnus			JX015409	Ilex aquifolium (holly)	Spain
			JX015410	Ilex aquifolium (holly)	Spain
R. paravitis	JX015422		JX015415	Helianthus annuus (sunflower)	Spain
			JX015416	Helianthus annuus (sunflower)	Spain
R. pumilus	JX015423	JX015435		Urtica sp.	USA
		JX015436		Urtica sp.	USA
R. rhomboides n. sp.	MK114123	MK114127	MK114144	Musa basjoo	Belgium
	MK114124	MK114128	MK114145	Musa basjoo	Belgium
	MK114125	MK114129	MK114146	Musa basjoo	Belgium
	MK114126	MK114130		Musa basjoo	Belgium
		MK114131		Musa basjoo	Belgium
		MK296738		Musa basjoo	Belgium
R. robustus	JX015424		JX015413	Pinus pinea (stone pine)	Spain
	JX015425	JX015438		Grasses	USA
	JX015426	JX015439	JX015411	Grasses	USA
		JX015440		Grasses	USA
			JX015414	Pinus pinea (stone pine)	Spain
R. sardashtensis	KP843115	KP843113		Unidentified grasses	Iran
R. striaticeps	KJ765343			Unidentified forest tree	Iran
	KJ765344			Unidentified forest tree	Iran
	KU722389			Unknown	Iran
			KP718973	Unknown	Iran
R. unisexus		EU373674		Citrus aurantium (citrus)	Spain
		EU373675		Citrus aurantium (citrus)	Spain
R. urmiaensis	KP718966		KP718971	Corylus spp.	Iran
	KP718967		KP718972	Corylus spp.	Iran
R. vitis	JN032581		JX015417	Vitis vinifera (grapevine)	Spain
			JX015418	Vitis vinifera (grapevine)	Spain
S. brachyurus			JX472089	Sansevieria laurentii	USA
S. truncatum			KX959308	Unknown	USA

Morphometric data of Rotylenchus rhomboides n_ sp_ All measurements are in μm (except for ratio) and in the form: mean ± sd (range)_

Character	Holotype female	Paratype females
n	1	12
Body length (L)	827	861 ± 101 (634–1049)
a = L/MBD	28.5	31.8 ± 2.8 (27.8–35.1)
b’ = L/Anterior end to the end of esophageal gland	7.6	6.9 ± 0.5 (5.6–7.6)
c = L/Tail length	55	63.3 ± 10.7 (50.3–85.5)
c’ = Tail length/ABD	68	0.7 ± 0.1 (0.5–0.8)
V = Anterior end to vulva/L × 100	59	56 ± 2.6 (53–61)
Lip height	6	6 ± 0.7 (5–7)
Lip width	11	10 ± 0.8 (8–11)
Stylet length	35	33 ± 1.5 (31–37)
Conus length	17	17 ± 0.8 (16–18)
Shaft length	14	13 ± 0.9 (12–14)
Knob height	3	3 ± 0.4 (3–4)
Dorsal gland opening from stylet base	4	3 ± 0.9 (2–5)
Anterior end to secretory-excretory pore	100	107 ± 10.6 (77–123)
Anterior end to nerve ring	78	86 ± 9.9 (60–96)
Anterior end to esophageal gland end	108	125 ± 10.5 (108–146)
Esophagus overlapping	12	14 ± 2.3 (9–19)
Max body diameter (MBD)	29	27 ± 2.3 (23–31)
Anal body diameter (ABD)	22	20 ± 1.8 (16–23)
Tail length	15	14 ± 2.0 (9–16)
Tail annuli number	11	10 ± 1.0 (8–11)

DOI: https://doi.org/10.21307/jofnem-2019-023 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 1 - 20

Submitted on: Dec 18, 2018

Published on: Apr 24, 2019

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

28S–D2D3,

Hierarchical cluster,

ITS,

Related subjects:

© 2019 Huu Tien Nguyen, Quang Phap Trinh, Marjolein Couvreur, Phougeishangbam Rolish Singh, Wilfrida Decraemer, Wim Bert, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)

Figures & Tables

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Morphometric data of Rotylenchus buxophilus from Belgium_ All measurements are in μm (except for ratio) and in the form: mean ± sd (range)_

Comparison of the matrix code of Rotylenchus rhomboides n_ sp_ with other closely related species_

The measurements of known and new populations of Rotylenchus buxophilus_

Rotylenchus species and populations used and sequenced in the present study_

Morphometric data of Rotylenchus rhomboides n_ sp_ All measurements are in μm (except for ratio) and in the form: mean ± sd (range)_

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