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Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India Cover

Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Open Access
|Apr 2019

Figures & Tables

Figure 1

Oscheius indicus n. sp. (A) Entire male; (B) Entire female; (C) Pharyngeal region; (D) Anterior region; (E) Female reproductive system; (F) Male posterior region showing spicules and gubernaculum; (G) Male tail in dorso-ventral view (genital papillae and bursa); (H) Female posterior region.
Oscheius indicus n. sp. (A) Entire male; (B) Entire female; (C) Pharyngeal region; (D) Anterior region; (E) Female reproductive system; (F) Male posterior region showing spicules and gubernaculum; (G) Male tail in dorso-ventral view (genital papillae and bursa); (H) Female posterior region.

Figure 2

Oscheius indicus n. sp. (A, B) Anterior region showing stoma; (C) Posterior pharynx.; (D) Genital papillae; (E) Anterior pharynx; (F) Vulva; (G) Male posterior region; (H) Female posterior region; (I) Lateral lines. (Scale bar: 20 μm).
Oscheius indicus n. sp. (A, B) Anterior region showing stoma; (C) Posterior pharynx.; (D) Genital papillae; (E) Anterior pharynx; (F) Vulva; (G) Male posterior region; (H) Female posterior region; (I) Lateral lines. (Scale bar: 20 μm).

Figure 3

Oscheius indicus n. sp. (A) Enface view showing six labial papillae (a to f), (B) Crochet needle-shaped spicules (C) Male posterior region showing genital papillae (GP1 to GP9), (D) Female posterior region showing anal opening (Scale bar: A = 2 μm; B & C = 10 μm, D = 5 μm).
Oscheius indicus n. sp. (A) Enface view showing six labial papillae (a to f), (B) Crochet needle-shaped spicules (C) Male posterior region showing genital papillae (GP1 to GP9), (D) Female posterior region showing anal opening (Scale bar: A = 2 μm; B & C = 10 μm, D = 5 μm).

Figure 4

Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using 28S D2/D3 extension region of 28 rDNA gene. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.
Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using 28S D2/D3 extension region of 28 rDNA gene. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.

Figure 5

Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using ITS region. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.
Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using ITS region. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.

Morphometric data of Oscheius indicus sp_ n_ Measurements are in µm and in the form: mean ± standard deviation (range)_

CharactersHolotype femaleParatype femalesParatype males
n11414
L1,3201,272.3 ± 111.7 (1,072–1,480)1,062.7 ± 104.5 (886–1,209)
a20.318.9 ± 1.1 (16.8–20.6)19.6 ± 1.6 (17.4–22.4)
b6.56.5 ± 0.4 (5.7–7.1)6.2 ± 0.6 (4.9–6.9)
c9.98.9 ± 0.9 (7.5–10.4)31.2 ± 2.8 (26.1–36.2)
c’5.35.8 ± 0.4 (5.0–6.6)1.3 ± 0.1 (1.2–1.4)
V50.248.2 ± 1.8 (45.4–50.5)
Maximum body diam.6567.8 ± 20.2 (59–82)54.4 ± 16.3 (46–64)
Lip width1212.6 ± 1.1 (11–15)11.6 ± 0.7 (11–13)
Length of stoma1615.4 ± 1.7 (13–18)15.6 ± 0.9 (14–17)
Corpus123117.7 ± 6.1 (108–127)105.4 ± 4.0 (101–114)
Isthmus4240.7 ± 3.4 (37–47)35.4 ± 3.5 (31–41)
Basal bulb length3736.4 ± 2.2 (33–41)29.9 ± 1.4 (27–32)
Basal bulb width2927.6 ± 1.8 (24–30)23.8 ± 0.9 (22–25)
Pharynx202194.8 ± 9.8 (181–210)172.4 ± 6.0 (159–181)
Excretory pore from anterior end201179.9 ± 15.1 (154–201)173.6 ± 9.7 (158–187)
Nerve ring from anterior end162142.0 ± 14.1 (118–162)136.7 ± 7.6 (122–146)
Anterior gonad524558.2 ± 43.2 (501–640)
Posterior gonad352504.4 ± 90.3 (324–614)
Vulva body diam.6466.4 ± 5.5 (58–80)
Vulva-anus distance525512.1 ± 40.2 (447–576)
Rectum7267.9 ± 5.8 (58–76)54.3 ± 4.3 (46–61)
Tail length133142.6 ± 8.9 (131–156)34.2 ± 2.3 (32–39)
Anal body diameter2524.8 ± 1.4 (23–28)25.6 ± 1.2 (24–28)
Pharynx gonad distance175118.0 ± 31.6 (89–175)82.8 ± 17.2 (61–116)
Testis_883.0 ± 82.1 (760–1,035)
Spicule length along axis_60.0 ± 4.2 (54–66)
Gubernaculum_25.1 ± 1.2 (24–28)
DOI: https://doi.org/10.21307/jofnem-2019-004 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 11
Submitted on: Sep 3, 2018
Published on: Apr 2, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2019 Puneet Kumar, Wajih Jamal, Vishal S. Somvanshi, Khushbu Chauhan, Sabia Mumtaz, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.