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The table shows the number of molecular differences in the 1_6kb SSU rRNA sequence for every pairwise comparison in the pacificus species-complex_
| P. pacificus | P. exspectatus | P. occultus | P. sikae | P. arcanus | P. kurosawai | P. taiwanensis | P. laevicollis sp. n. | P. maxplancki | P. riukiariae sp. n. | P. neolucani sp. n. | P. degawai sp. n. | P. hongkongensis sp. n. | P. quartusdecimus | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P. pacificus | 0 | 8 | 5 | 10 | 6 | 3 | 6 | 21 | 16 | 35 | 25 | 31 | 42 | 36 |
| P. exspectatus | – | 0 | 6 | 12 | 8 | 5 | 8 | 23 | 16 | 34 | 27 | 33 | 43 | 38 |
| P. occultus | – | – | 0 | 1 | 4 | 2 | 5 | 18 | 14 | 27 | 21 | 27 | 38 | 33 |
| P. sikae | – | – | – | 0 | 10 | 1 | 4 | 18 | 14 | 32 | 21 | 27 | 38 | 33 |
| P. arcanus | – | – | – | – | 0 | 3 | 4 | 19 | 14 | 31 | 24 | 30 | 41 | 35 |
| P. kurosawai | – | – | – | – | – | 0 | 3 | 18 | 13 | 28 | 22 | 28 | 39 | 33 |
| P. taiwanensis | – | – | – | – | – | – | 0 | 19 | 14 | 29 | 24 | 30 | 41 | 35 |
| P. laevicollis sp.n. | – | – | – | – | – | – | – | 0 | 18 | 35 | 34 | 36 | 47 | 42 |
| P. maxplancki | – | – | – | – | – | – | – | – | 0 | 29 | 28 | 30 | 41 | 39 |
| P. riukiariae sp.n. | – | – | – | – | – | – | – | – | – | 0 | 5 | 4 | 14 | 19 |
| P. neolucani sp.n. | – | – | – | – | – | – | – | – | – | – | 0 | 6 | 17 | 18 |
| P. degawai sp.n. | – | – | – | – | – | – | – | – | – | – | – | 0 | 14 | 19 |
| P. hongkongensis sp.n. | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 23 |
| P. quartusdecimus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Morphometrics of P_ honkongensis sp_ n_ and P_ neolucani sp_ n_
| Character | P. hongkongensis RS5957 stenostomatous male | Stenostomatous female | P. neolucani RS5949 stenostomatous male | Stenostomatous female |
|---|---|---|---|---|
| n | 10 | 10 | 10 | 10 |
| L | 1014 ± 185 (700–1302) | 1181 ± 315 (918–2008) | 871 ± 49.6 (775–951) | 1060 ± 111.1 (930–1297) |
| L’ | 847 ± 184 (560–1125) | 940 ± 288 (703–1700) | 745 ± 50.7 (658–826) | 864 ± 103.2 (743–1080) |
| a | 14 ± 3.7 (11–24) | 13 ± 0.9 (12–15) | 15 ± 1 (14–16) | 14 ± 0.7 (13–15) |
| b | 6 ± 1 (4.6–7.7) | 6.6 ± 1.1 (5.6–9.5) | 5.4 ± 0.6 (4.7–6.6) | 5.5 ± 0.4 (4.8–6.5) |
| c | 6.1 ± 1.3 (5–8.5) | 4.8 ± 0.7 (4.3–6.5) | 7 ± 0.9 (6.1–9.2) | 5.4 ± 0.5 (4.5–6.1) |
| c’ | 4.2 ± 0.9 (3–5.9) | 6.3 ± 0.5 (5.1–6.9) | 3.7 ± 0.7 (2.6–4.9) | 6.1 ± 0.9 (5–7.8) |
| T or V | 53 ± 4.8 (51–68) | 45 ± 2.6 (42–50) | 53 ± 7.5 (33–59) | 47 ± 1.3 (45–48) |
| Maximum body diam. | 73 ± 14.9 (51–96) | 92 ± 29.4 (67–170) | 57 ± 5.8 (49–67) | 75 ± 9 (63–95) |
| Stoma length | 12.6 ± 1 (11.4–14.6) | 13.3 ± 1.6 (10.3–16) | 10.4 ± 1 (8.7–12) | 11 ± 0.9 (10.2–13) |
| Stoma diam. | 7.2 ± 0.7 (6.1–8.5) | 7.7 ±1.1 (6.5–9.7) | 6.0 ± 0.7 (5.3–7.6) | 7.1 ± 0.7 (6.3–8.4) |
| Pharynx length (head to base of pharynx) | 155 ± 9.7 (140–172) | 168 ± 14 (154-202) | 151 ± 11.9 (129-169) | 182 ± 14.9 (149-194) |
| Anterior pharynx (pro- + metacorpus) | 98 ± 5.4 (90–106) | 106 ± 7.4 (94–117) | 95 ± 10.2 (76–112) | 116 ± 9.2 (97–128) |
| Posterior pharynx (isthmus + basal bulb) | 57 ± 5.9 (49–66) | 62 ± 8.4 (56–85) | 56 ± 5.3 (51–69) | 66 ± 7.1 (52–75) |
| Ant/total pharynx % | 63 ± 2 (60–66) | 63 ± 2.5 (58–66) | 63 ± 3.2 (59–67) | 64 ± 1.8 (61– 66) |
| Median bulb diam. | 27 ± 2 (24–31) | 31 ± 3.6 (26–38) | 25 ± 1 (24–27) | 31 ± 2.9 (27–36) |
| Terminal bulb diam. | 26 ± 2.2 (22–28) | 29 ± 6.6 (24–47) | 25 ± 1.3 (22–27) | 29 ± 1.7 (27–33) |
| Testis length | 540 ± 141 (366–765) | – | 467±43 (425–561) | – |
| Ant. end to vulva | – | 539 ± 172 (384–995) | – | 493 ± 50.9 (420–581) |
| Vulva to anus distance | – | 419 ± 149 (320–815) | – | 371 ± 47 (290–447) |
| Cloacal or anal body diam. | 41 ± 5.4 (30–50) | 39 ± 8 (32–60) | 35 ± 4.8 (27–43) | 32 ± 3.8 (27–38) |
| Tail length | 167 ± 21.6 (140–206) | 241 ± 30 (215–308) | 125 ± 13.2 (99–148) | 196 ± 19 (175–228) |
| Spicule length (curve) | 53 ± 4.2 (44–57) | – | 47±2.3 (43–51) | – |
| Spicule length (chord) | 45 ± 3.7 (37–49) | – | 36 ± 1.7 (34–39) | – |
| Gubernaculum length | 19 ± 1.8 (17–22) | – | 17 ± 1.5 (14–19) | – |
Morphometrics of P_ riukiariae sp_ n_ and P_ degawai sp_ n_
| Character | P. riukiariae RS5937 stenostomatous male | Stenostomatous female | P. degawai RS5938 stenostomatous male | Stenostomatous female |
|---|---|---|---|---|
| n | 10 | 10 | 10 | 10 |
| L | 918 ± 162 (663–1180) | 1362 ± 211 (1025–1700) | 872 ± 142.2 (710–1105) | 1184 ± 310.5 (811–1853) |
| L’ | 780 ± 151 (553–1010) | 1142 ± 201 (835–1466) | 727 ± 134.6 (585–961) | 986 ± 282 (652–1600) |
| a | 12 ± 2.5 (9.4–15) | 14 ± 1.5 (11–16) | 16 ± 2 (13–19) | 16 ± 3 (132–23) |
| b | 5.7 ± 0.8 (5–7.5) | 8.4 ± 1.5 (5.9–10.8) | 5.3 ± 0.6 (4.6–6.4) | 6.6 ± 1.2 (5.1–8.6) |
| c | 6.8 ± 1.2 (5.4–9.3) | 6.2 ± 0.7 (5–7.3) | 6 ± 0.8 (5.4–7.7) | 6 ± 0.9 (4.7–7.3) |
| c’ | 3.4 ± 0.4 (2.7–4.2) | 5.2 ± 0.6 (4.5–6.1) | 4.1 ± 0.5 (3.3–4.7) | 5.2 ± 1 (3.8–7.3) |
| T or V | 51 ± 5.3 (41–59) | 45 ± 11.1 (32–72) | 45 ± 5 (40–56) | 51 ± 5.5 (45–63) |
| Maximum body diam. | 78 ± 13.2 (62–97) | 99 ± 19.4 (65–128) | 56 ± 14.4 (40–80) | 80 ± 28.6 (37–138) |
| Stoma length | 11.1 ± 1 (9.7–12.5) | 11.8 ± 1.3 (9.2–14) | 11.4 ± 0.8 (10–12.3) | 12.1 ± 0.9 (11–13.4) |
| Stoma diam. | 6.5 ± 0.8 (5.4–7.6) | 7.3 ±0.7 (6.2–8.4) | 6.7 ± 0.3 (6.3–7) | 7.6 ± 0.6 (6.9–8.7) |
| Pharynx length (head to base of pharynx) | 143 ± 17.6 (111–167) | 156 ± 12.2 (136–178) | 152 ± 10.1 (143–176) | 169 ± 16.3 (148–207) |
| Anterior pharynx (pro– + metacorpus) | 94 ± 11 (78–111) | 97 ± 8.8 (86–116) | 95 ± 4.2 (89–103) | 106 ± 8.4 (96–122) |
| Posterior pharynx (isthmus + basal bulb) | 54 ± 5.8 (43–63) | 58 ± 7 (40–66) | 58 ± 7.1 (47–73) | 62 ± 9.6 (51–85) |
| Ant/total pharynx % | 67 ± 11.9 (59–100) | 62 ± 3.4 (60–71) | 62 ± 2.4 (59–67) | 64 ± 2.3 (60– 68) |
| Median bulb diam. | 27 ± 2.2 (23–31) | 31 ± 1.4 (29–33) | 26 ± 3 (22–32) | 30 ± 4.6 (24–41) |
| Terminal bulb diam. | 24 ± 2.8 (19–28) | 28 ± 2.3 (24–32) | 25 ± 4 (19–33) | 28 ± 4.8 (22–39) |
| Testis length | 468 ± 116.4 (295–677) | – | 397 ± 105.7 (285–580) | – |
| Ant. end to vulva | – | 624 ± 199 (415–1040) | – | 601 ± 173.3 (416–1043) |
| Vulva to anus distance | – | 510 ± 94.6 (332–665) | – | 365 ± 72.8 (255–487) |
| Cloacal or anal body diam. | 41 ± 4.5 (32–46) | 43 ± 6.1 (31–52) | 35 ± 5.6 (30–44) | 39 ± 8.7 (22–55) |
| Tail length | 137 ± 21.2 (104–170) | 220 ± 14.3 (190–241) | 145 ± 14.5 (125–180) | 198 ± 36.8 (150–253) |
| Spicule length (curve) | 53 ± 5.5 (40–60) | – | 47±7.6 (37–57) | – |
| Spicule length (chord) | 43 ± 3.4 (36–47) | – | 40 ± 6.9 (32–50) | – |
| Gubernaculum length | 12 ± 2.5 (9.4–15) | – | 16 ± 2.4 (13–19) | – |
Morphometrics of P_ laevicollis sp_ n_
| Character | P. laevicollis RS5939 stenostomatous male | Stenostomatous female |
|---|---|---|
| n | 10 | 10 |
| L | 834 ± 104 (662–971) | 1104 ± 209 (746–1325) |
| L’ | 705 ± 89.6 (566–836) | 908 ± 179 (595–1126) |
| a | 15 ± 1 (13–17) | 13 ± 0.8 (12–15) |
| b | 6.5 ± 0.5 (5.9–7.4) | 7.6 ± 0.8 (5.8–8.6) |
| c | 6.5 ± 0.4 (5.9–7.2) | 5.7 ± 0.7 (4.6–6.9) |
| c’ | 3.9 ± 0.4 (3–4.3) | 5.8 ± 0.7 (4.9–7.1) |
| T or V | 59 ± 2 (56–62) | 46 ± 2.1 (42–49) |
| Maximum body diam. | 57 ± 7.4 (47–66) | 83 ± 14.8 (57–97) |
| Stoma length | 9.4 ± 1 (7.7–10.6) | 11.1 ± 1.1 (9–12.5) |
| Stoma diam. | 5.6 ± 0.7 (4–6.4) | 6.4 ± 0.7 (5.5–8.0) |
| Pharynx length (head to base of pharynx) | 118 ± 9.5 (103–132) | 137 ± 15.5 (113–154) |
| Anterior pharynx (pro– + metacorpus) | 72 ± 6.7 (61–81) | 84 ± 8.7 (71–93) |
| Posterior pharynx (isthmus + basal bulb) | 46 ± 2.9 (42–51) | 53 ± 7.1 (42–63) |
| Ant/total pharynx % | 61 ± 1 (58–61) | 61 ± 1.2 (59–63) |
| Median bulb diam. | 20 ± 2.1 (16–22) | 27 ± 3.1 (22–30) |
| Terminal bulb diam. | 19 ± 1.7 (16–21) | 25 ± 3.8 (19–31) |
| Testis length | 490 ± 72.5 (370–570) | – |
| Ant. end to vulva | – | 504±86.2 (365–613) |
| Vulva to anus distance | – | 410 ± 78.9 (275–523) |
| Cloacal or anal body diam. | 33 ± 2.3 (28–36) | 34 ± 4.5 (32–60) |
| Tail length | 129 ± 17.4 (96–152) | 196 ± 47.1 (138–285) |
| Spicule length (curve) | 44 ± 2.5 (40–47) | – |
| Spicule length (chord) | 35 ± 2.1 (31–38) | – |
| Gubernaculum length | 16 ± 1 (14–17) | – |
Summary of diagnostic characters of the Pristionchus pacificus complex sensu stricto_
| Stenostomatous form | Eurystomatous form | ||||||
|---|---|---|---|---|---|---|---|
| Species | Reproductive mode | Right subventral plate | Left subventral plate | Right subventral tooth | Left subventral cusps | Genital papillae | Other characteristic feature(s) |
| pacificus | H | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Exspectatus | G | Single peak | Three bumps | Claw-like | three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Occultus | G | single peak | three bumps | claw-like | Three large cusps each sometimes split into two to three (= 3-9 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Sikae | G | Single peak | Three bumps | Claw-like with or without blunt projection | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Arcanus | G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 2: v1-v2d > v2d-v4; v2d and v3 are close to each other | |
| Kurosawai | G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Taiwanensis | G | Single peak | Three bumps | Claw-like with blunt projection | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Laevicollis n. sp. | G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 3: v1-v2 = v2-v4; v2 and v3d are separated | |
| maxplancki | G | 1-2 peaks | with minute denticles | claw-like | three large cusps each sometimes split into two (= 3-6 peaks) | Type 3: v1-v2 = v2-v4; v2 and v3d are separated | |
| Japonicus | G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 4: v1-v2 < v2-v4; v2 and v3d are separated | |
| Riukiariae n. sp. | G | Two peaks | Three bumps | Two large ridges or multiple small denticles | Three large cusps each sometimes split into two to three (= 3-9 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Neolucani n. sp. | G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
| Degawai n. sp. | G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | v1-v2d = v2d-v4; v2d and v3 are close to each other | v2 and v3 are very close, and sometimes third pair is directed sublaterally (v3d) |
| Hongkongensis n. sp. | G | Single peak | Three bumps | Claw-like | Three large cusps each split into three or more (= more than 9 peaks) | v1-v2 < v2-v4; v2 and v3d are separated | Comparatively large and slightly more barrel-shaped stoma in eurystomatous form |
| Quatrusdecimus | G | 1-2 peaks | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | v1-v2 < v2-v4; v2 and v3d are separated | |