Table 1
Cost estimates for the possible EPN-augmentation approaches in guava orchards to achieve maximum suppression of Caribfly at the best IJ ratea.
| Strategic EPN-augmentation option | In series, number of fruit tree−1 on the ground | Total number of fruit on the ground in 1 hectareb | Required IJs ha−1 of Heterorhabditis bacteriophora | Estimated cost of total number of IJs (US$ ha−1) |
|---|---|---|---|---|
| Hectare-wide EPN-augmentation or broadcasting at ‘(50 IJs cm−2 spread over a fruit in 350 cm2)’ seems to be less cost-effective | na | na | 5 × 109 | 970c |
| Applying Heterorhabditis bacteriophora IJs over each infested fruit on soil surface at 17,500 IJs fruit−1 seems to be more economical | ⩽1 | ⩽500 | 8,750,000 | 1.70 |
| 2 | 1,000 | 17,500,000 | 3.40 | |
| 3 | 1,500 | 26,250,000 | 5.10 | |
| 4 | 2,000 | 35,000,000 | 6.79 | |
| 5e | 2,500 | 43,750,000 | 8.49 | |
| 6 | 3,000 | 52,500,000 | 10.19 | |
| 7 | 3,500 | 61,250,000 | 11.88 | |
| 8 | 4,000 | 70,000,000 | 13.58 | |
| 9 | 4,500 | 78,750,000 | 15.28 | |
| 10 | 5,000 | 87,500,000 | 16.98 | |
| The average costs of Heterorhabditis bacteriophora IJs ha−1 in each month | 9.34d | |||
[i] na: Not applicable, because guava fruit that drop to the ground are not observed in every 1 cm2 soil surface in infested guava orchards; aThe equivalent best IJ rate for EPN treatment to an infested fruit in plot (i.e., 350 cm2) at 50 IJs cm−2 is 17,500 IJs fruit−1 (Figures 1, 2); bAdditional 28% was included to account for unexpected uncertainties or increases that may rarely occur from March to July; cAnnual cost estimates for hectare-wide EPN-augmentation or broadcasting strategy in the period from March-July = 970 × 5 ≈ US$ 4,850 ha−1; dAverage annual cost estimates for spot treatment method in March-July period = (9.34 + four-hour-labor costs) × 5 ≈ US$ 266.70 ha−1; eFrom March to July, the ratios between 0 and 5 fruit tree−1 on any given day will often be observed on the ground in guava orchards (Heve et al., 2018).

Figure 1
The patterns of numbers of emerging (A) adult Caribfly and (B) parasitoid wasps (Diachasmimorpha longicaudata) observed in EPN treatments, with increasing IJ rate of Heterorhabditis bacteriophora applied over two Caribfly-infested guavas in the field. Tukey’s HSD tests at P ⩽ 0.05: same letter on top of bars (± standard errors) denotes no significant differences, even though numerical margins were observed, in accumulated densities of emerging adult Caribfly or the parasitoid wasps between application rates of Heterorhabditis bacteriophora.

Figure 2.
Relationship between relative survival of Caribfly and increasing IJ rate of Heterorhabditis bacteriophora used to treat two infested guavas plot−1 in field trials from June to August 2017. The coefficient of determination and the probability statistic for significance level are R 2 and P, respectively.
Table 2
Cost-benefit analysis showing changing annual base net income level in response to varying total costs following inclusion of Heterorhabditis bacteriophora in Caribfly IPM plans to suppress Caribfly (Anastrepha suspensa) better in south Florida guava orchards.
| Methods to include Heterorhabditis bacteriophora in Caribfly IPM | |||
|---|---|---|---|
| Before inclusion of Heterorhabditis bacteriophora in IPM | Spot treatment technique | Hectare-wide broadcasting | |
| Details | US$ ha−1 | US$ ha−1 | US$ ha−1 |
| 1. Production costsa | |||
| Irrigation | 494 | 494 | 494 |
| Fertilizers | 1,359 | 1,359 | 1,359 |
| Insecticides | 1,112 | 1,112 | 1,112 |
| Herbicides | 1,112 | 1,112 | 1,112 |
| Fungicides | 988 | 988 | 988 |
| Heterorhabditis bacteriophora (in Table 1) | 0 | 267 | 4,850 |
| Labor (pruning; supervision; others) | 1,606 | 1,606 | 1,606 |
| Interest on capital (5%) | 334 | 347 | 576 |
| 2. Fixed costs | |||
| Cash overhead: | |||
| Insurance | 247 | 247 | 247 |
| Taxes | 247 | 247 | 247 |
| Non-cash overhead: | |||
| Land rent | 1,236 | 1,236 | 1,236 |
| Other overhead | 1,483 | 1,483 | 1,483 |
| 3. Harvesting and marketing costs | |||
| Picking and sales cost | 6,919 | 6,919 | 6,919 |
| 4. Total costs | 17,137 | 17,417 | 22,229 |
| 5. Returns on ‘wholesale base yield’ | |||
| Annual wholesale base revenueb, c | 39,537 | 39,537 | 39,537 |
| Annual base net incomed | 22,400 | 22,120 | 17,308 |
[i] a Heterorhabditis bacteriophora and insecticides were considered to effectively suppress fruit-to-soil stages of Caribfly belowground and their adults aboveground, respectively, because other Caribfly control approaches are rarely observed in south Florida guava orchards due largely to high costs involved; b Annual wholesale base revenue = ‘Annual wholesale base yield (ca. 8,967 kg ha−1) times base price (US$ 4.41 kg−1)’ is equivalent to US$ 16,000 acre−1 in report of Evans et al. (2018); c These are largely non-susceptible mature hard dark green guavas; susceptible “light-green-to-yellow” ripening guavas lost annually are estimated to be (10,930 kg ha−1 minus ca. 8,967 kg ha−1 ≈ 1,963 kg ha−1), equivalent to US$ 8,650 ha−1; d Additional information can be obtained from reports of Garcia et al. (2014) and Evans et al. (2018).

Figure 3.
Linear relationship between cumulative densities of emerging adult Caribfly and those of the surviving parasitoid wasps, Diachasmimorpha longicaudata. The coefficient of determination is R 2 whereas P is the probability statistic for significance level.

Figure 4.
Relationship between probability of observing large numbers of the parasitoid Diachasmimorpha longicaudata and the increasing IJ rate from 0 to 100 IJs cm−2, following EPN-augmentation of Heterorhabditis bacteriophora over two guavas in field trials from June to August 2017. The coefficient of determination is R 2, whereas the probability statistic for significance level is P.