Induction of 2n pollen by colchicine in Populus × popularis and its triploids breeding

Xi, X. J.; Jiang, X. B.; Li, D.; Guo, L. Q.; Zhang, J. F.; Wei, Z. Z.; Li, B. L.

doi:10.1515/sg-2011-0021

Abstract

Induction of 2n pollen is a required technique for cultivating polyploid via sexual polyploidy. Orthogonal design or Taguchi Design was applied to select the best treatment process of 2n pollen induction in Populus × popularis from different levels of the meiosis stage of male flower buds, colchicine concentration, times of injection, and interval between injections. Flow cytometry and chromosome counting were used to identify the triploids from the offspring of P. × euramericana. (Dode) Guinier pollinated with induced pollen of P. × popularis. The results showed that high 2n pollen rate can be achieved by selecting the flower buds during diakinesis stage in meiosis, and then injecting 0.6% colchicine 4 times with 2 hours interval. The 2n pollen rate reached 62.10% by this process, and two triploids were obtained, which indicates that it is possible for cultivating triploids via 2n pollen induction by colchicine treatment in poplar. Results and protocol related to 2n pollen induction, polyploid identification and effect of 2n pollen in this study might be applicable in polyploidy breeding in section Aigeiros and Tacamahaca of poplar.

References

Bradshaw, H. D. and R. F. Stettler (1993): Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars. Theoretical and Applied Genetics 86: 301–307.10.1007/BF0022209224193473
Search in Google Scholar Back to article
Chen, Z. J. and Z. F. Ni (2006): Mechanisms of genomic rearrangements and gene expression changes in plant polyploids. BioEssays 28(3): 240–252.10.1002/bies.20374198666616479580
Search in Google Scholar Back to article
Contreras, R. N., T. G. Ranney and S. P. Tallury (2007): Reproductive behavior of diploid and allotetraploid Rhododendron L. ‘Fragrant Affinity’. HortScience 42: 31–34.10.21273/HORTSCI.42.1.31
Search in Google Scholar Back to article
Darzynkiewicz, Z. and G. Juan (2001): DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis, pp. 1–24. In: Current Protocols in Cytometry, edited by J. P. Robinson, John Wiley & Sons Inc, New York.
Search in Google Scholar Back to article
Doležel, J., P. Binarová and S. Lucretti (1989): Analysis of nuclear DNA content in plant cells by flow cytometry. Biologia Plantarum 31(2): 113–120.10.1007/BF02907241
Search in Google Scholar Back to article
Eigsti, O. J. and P. Dustin (1955): Colchicine in agriculture, medicine, biology, chemistry. Iowa State College Press, Iowa.10.31274/isudp.1955.89
Search in Google Scholar Back to article
Ewald, D., K. Ulrich, G. Naujoks and M. B. Schröder (2009): Induction of tetraploid poplar and black locust plants using colchicine: chloroplast number as an early marker for selecting polyploids in vitro. Plant Cell, Tissue and Organ Culture 99(3): 353–357.10.1007/s11240-009-9601-3
Search in Google Scholar Back to article
Hancock, J. F. (1997): The colchicine story. HortScience 32: 1011–1012.10.21273/HORTSCI.32.6.1011
Search in Google Scholar Back to article
Huang, Q. J., Z. Y. Zhang and X. Y. Kang (2002): 2n pollen of P. tomentosa × P. bolleana induced by four antimicrotubule agents. Journal of Beijing Forestry University 24: 12–15.
Search in Google Scholar Back to article
Kang, X. Y., J. Zhang, P. D. Zhang and Y. H. Li (2006): Induction of triploids in white poplar, pp. 71 in International Poplar Symposium IV., Nanjing.
Search in Google Scholar Back to article
Kang, X. Y., Z. T. Zhu and H. B. Lin (2000a): Radiosensitivity of different ploidy pollen in white poplar and its application. Acta Genetica Sinica 27: 78–82.
Search in Google Scholar Back to article
Kang, X. Y., Z. T. Zhu and Z. Y. Zhang (2000b): Meiosis and its stages of pollen mother cells in Chinese white poplar. Journal of Beijing Forestry University 22: 5–7.
Search in Google Scholar Back to article
Li, M. and Z. Zhang (1996): Crop chromosome and its research technique. China Agricultural Press, Beijing.
Search in Google Scholar Back to article
Mashkina, O. S., L. M. Burdaeva and M. M. Belozerova (1989): Method of obtaining diploid pollen of woody species. Lesovedenie 1: 19–25.
Search in Google Scholar Back to article
Müntzing, A. (1936): The chromosome of a giant Populus tremula. Hereditas 21: 383–393.10.1111/j.1601-5223.1936.tb03206.x
Search in Google Scholar Back to article
Ni, Z. F., E. D. Kim, M. Ha, E. Lackey, J. X. Liu and Y. R. Zhang et al. (2009): Altered circadian rhythms regulate growth vigor in hybrids and allopolyploids. Nature 457(7227): 327–331.10.1038/nature07523267970219029881
Search in Google Scholar Back to article
Nilsson-Ehle, H. (1936): Über eine in der Natur gefundene Gigasform van Populus tremula. Hereditas 21: 379–382.10.1111/j.1601-5223.1936.tb03205.x
Search in Google Scholar Back to article
Olsen, R. T., T. G. Ranney and Z. Viloria (2006): Reproductive behavior of induced allotetraploid × Chitalpa and in vitro embryo culture of polyploid progeny. Journal of the American Society for Horticultural Science 131(6): 716–724.10.21273/JASHS.131.6.716
Search in Google Scholar Back to article
Qi, C. L., K. L. Li, J. Xiao, Z. X. Li, C. L. Jin and W. H. Teng (2009): Meiosis of Pollen Mother Cells and Pollen Development of Populus ussuriensis. Journal of Northeast Forestry University 37: 72–75.
Search in Google Scholar Back to article
Ramsey, J. and D. W. Schemske (1998): Pathways, mechanisms, and rates of polyploidy formation in flowering plants. Annual Review of Ecology and Systematics 29: 467–501.10.1146/annurev.ecolsys.29.1.467
Search in Google Scholar Back to article
Ranney, T. G. (2006): Polyploidy: From evolution to new plant development. Combined Proceedings, International Plant Propagators’ Society 56: 137–142.
Search in Google Scholar Back to article
Soltis, D. E., P. S. Soltis and J. A. Tate (2004): Advances in the study of polyploidy since Plant Speciation. New Phytologist 161(1): 173–191.10.1046/j.1469-8137.2003.00948.x
Search in Google Scholar Back to article
Vuchkov, I. N. and L. N. Boyadjieva (2001): Quality Improvement with Design of Experiments: A Response Surface Approach. Springer-Verlag, Berlin.10.1007/978-94-009-0009-7
Search in Google Scholar Back to article
Watanabe, K., H. M. Ei-Nashaar and M. Iwanaga (1992): Transmission of bacterial wilt resistance by first division restitution (FDR) 2n pollen via 4x × 2x crosses in potatoes. Euphytica 60(1): 21–2.10.1007/BF00022254
Search in Google Scholar Back to article
Wendel, J. F. (2000): Genome evolution in polyploids. Plant Molecular Biology 42(1): 225–249.10.1023/A:1006392424384
Search in Google Scholar Back to article
Xu, J. R. and Z. H. Huang (1999): Forestry Experimental Design. Chinese Forestry Publishing House, Beijing.
Search in Google Scholar Back to article
Zhang, J. F., Z. Z. Wei, D. Li and B. L. Li (2009): Using SSR markers to study the mechanism of 2n pollen formatiom in Populus × euramericana (Dode) guinier and P. × popularis. Annals of Forest Science 66(5): 53–62.
Search in Google Scholar Back to article
Zhang, S., L. Qi, C. Chen, X. Li, W. Song and R. Chen et al. (2004): A report of triploid Populus of the section Aigeiros. Silvae Genetica 53(2): 69–75.10.1515/sg-2004-0013
Search in Google Scholar Back to article
Zhu, Z. T., H. B. Lin and X. Y. Kang (1995): Studies on allotriploid breeding of Populus tomentosa B301 clones. Scientia Silvae Sinicae 31(6): 499–505.
Search in Google Scholar Back to article

Induction of 2n pollen by colchicine in Populus × popularis and its triploids breeding

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