Short note: Development of Six EST-SSR Markers in Larch

Yang, X.; Sun, X.; Zhang, S.

doi:10.1515/sg-2011-0022

Abstract

Six simple sequence repeats (SSR) markers were developed from expressed sequence tags (ESTs) in the genus Larix. Based on evaluation with 49 L. kaempferi genotypes, the number of alleles per locus ranged from two to four, and the expected (He) and observed (Ho) heterozygosity values were 0.225−0.694 and 0.201−0.656, respectively. The inbreeding coffcient (F_IS) for all loci were less than zero except that LAReSSR85 was 0.4383. All the six EST-SSR markers were transferable to L. gmelini, L. olgensis var Koreana, L. principisrupprechtii and L. olgensis. BlastX analysis showed that five of the EST-SSRs were homologous to known genes. The six EST-SSR markers developed here can be valuable for biological applications in Larix.

References

Agendae Academiae Sinicae (1978): Flora Reipublicae Popularis Sinicae: Tomus 7. Science Press, Beijing, China.
Search in Google Scholar Back to article
Chen, C., C. Liewlaksaneeyanawin, T. Funda, A. Kenawy, C. H. Newton and Y. A. El-Kassaby (2009): Development and characterization of microsatellite loci in western larch (Larix occidentalis Nutt.). Mol. Ecol. Resour. 9: 843–845.
Search in Google Scholar Back to article
Doyle, J. J. and J. L. Doyle (1990): Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
Search in Google Scholar Back to article
Isoda, K. and A. Watanabe (2006): Isolation and characterization of microsatellite loci from Larix kaempferi. Mol. Ecol. Notes 6: 664–666.
Search in Google Scholar Back to article
Ji, Y.-Z., L.-X. Chen, B.-M. Xue and W.-Z. Feng (2004): Influence of fertilization on plant nutrition and physiological characteristics in larch plantations. Ecol. Environ. 13: 217–219.
Search in Google Scholar Back to article
Khasa, P. D., C. H. Newton, M. H. Rahman, B. Jaquish and B. P. Dancik (2000): Isolation, characterization, and inheritance of microsatellite loci in alpine larch and western larch. Genome 43: 439–448.10.1139/g99-13110902706
Search in Google Scholar Back to article
Leng, W.-F., H.-S. He, R.-C. Bu and Y.-M. Hu (2007): Sensitivity analysis of the impacts of climate change on potential distribution of three larch (Larix) species in northeastern China. J. Plant Ecol. 31: 825–833.
Search in Google Scholar Back to article
Ma, C. G. and X. M. Sun (2008): Larch genetic improvement and its future development in China. World For. Res. 21(3): 58–63.
Search in Google Scholar Back to article
Varshney, R. K., A. Graner and M. E. Sorrells (2005): Genic microsatellite markers in plants: features and applications. Trends Biotechnol. 23: 48–55.10.1016/j.tibtech.2004.11.00515629858
Search in Google Scholar Back to article
Yeh, F. C., R. Yang and T. Boyle (1999): POPGENE version 1.32. Microsoft window-based freeware for population genetic analysis. University of Alberta, Edmonton, Canada.
Search in Google Scholar Back to article
Zhang, S.-G., W.-H. Yang, M.-X. Li, S.-Y. Han, J.-H. Wang, X.-M. Sun, X.-S. Wang and L.-W. Qi (2007): Studies on the pollen mother cell meiosis and pollen development of Larix principis-rupprechtii. Acta Phytotaxonomica Sinica 45: 505–512.10.1360/aps06124
Search in Google Scholar Back to article

Short note: Development of Six EST-SSR Markers in Larch

Abstract

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