Separation of matrine and oxymatrine from Sophora flavescens extract through cation exchange resin coupled with macroporous absorption resin

Chen, Haohao; Luo, Shaojian; Zheng, Xikang; Fan, Huajun

doi:10.1515/pjct-2016-0026

Abstract

A simple method for separation of matrine and oxymatrine from Sophora flavescens was developed with cation exchange resin coupled with macroporous resin. Based on the adsorption characteristics of matrine and oxymatrine, 001×732 cation exchange resin was used to absorb target alkaloids for removing most of the foreign matter, while BS-65 macroporous resin was chosen to purify these alkaloids. The result showed that the equilibrium adsorption data of matrine and oxymatrine on 001×732 resin and BS-65 resin at 30°C was fitted to Langmuir isotherm and Freundlich isotherm, respectively. The contents of matrine and oxymatrine were increased from 0.73% and 2.2% in the crude extract of the root of Sophora flavescens to 67.2% and 66.8% in the final eluent products with the recoveries of 90.3% and 86.9%, respectively.

References

1. Pharmacopoeia Commission of People’s Republic of China. (2010). Pharmacopoeia of People’s Republic of China (188). Beijing: The Chemical Industry Press.
Search in Google Scholar
2. Song, G.B., Luo, Q., Qin, J., Wang, L., Shi, Y.S. & Sun, C.X. (2006). Effects of oxymatrine on proliferation and apoptosis in human hepatoma cells. Coll. Surf. B. 48, 1–5. DOI: 10.1177/1533034615587616.10.1177/153303461558761626009496
Search in Google Scholar
3. Ueng, Y.F., Chen, C.C., Tsai, C.C. & Souček, P. (2009). Different inductive profiles of hepatic cytochrome P450s by the extracts of Sophora flavescens in male and female C57BL/6JNarl mice. J. Ethnopharmac. 126, 437–446. DOI: 10.1016/j.jep.2009.09.018.10.1016/j.jep.2009.09.01819772910
Search in Google Scholar
4. Wan, X.Y., Luo, M., Li, X.D. & He, P. (2009). Hepatoprotective and anti-hepatocarcinogenic effects of glycyrrhizin and matrine. Chem. Biol. Interact. 181, 15–19. DOI: 10.1016/j.cbi.2009.04.013.10.1016/j.cbi.2009.04.01319426721
Search in Google Scholar
5. Luo, C., Zhu, Y.L., Jiang, T.J., Lu, X.Y., Zhang, W., Jing, Q.F., Li, J., Pang, L.R., Chen, K.J., Qiu, F.M., Yu, X.Y., Yang, J.H. & Huang, J. (2007). Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. Toxicol. Lett. 229, 245–252. DOI: 10.1016/j.tox.2006.10.020.10.1016/j.tox.2006.10.02017134813
Search in Google Scholar
6. Suo, Z.W., Liu, Y., Ferreri, M., Zhang, T., Liu, Z.J., Mu, X. & Han, B. (2009) Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells. J. Ethnopharmacl. 125, 404–409. DOI: 10.1016/j.jep.2009.07.023.10.1016/j.jep.2009.07.02319635549
Search in Google Scholar
7. Gao, H.Y., Li, G.Y., Lou, M.M., Li, X.Y., Wei, X.Y. & Wang, J.H. (2012). Hepatoprotective effect of Matrine salvianolic acid B salt on Carbon Tetrachloride – Induced Hepatic Fibrosis. J. Inflamm. 9, 16. DOI: 10.1186/1476-9255-9-16.10.1186/1476-9255-9-16340402022559721
Search in Google Scholar
8. Zhang, H.L., He, C.L., Yang, Q.E., Yang, X.J. & Han, C.X. (2006). Study on separation and toxicity to Mus musculus of oxymatrine. Nat. Prod. Res. Dev. 18, 279–281. DOI: 1001-6880(2006)02-0279-04.
Search in Google Scholar
9. Hu, Y.L., Guo, L. & Jia, X.H. (2007). Study on separation and purification technology of Sophara alopecuroides L. Food Sci. 28, 32–36. DOI: 1002-6630(2007)01-0032-05.
Search in Google Scholar
10. An, G. (2006). Studies on technology for extracting and purifying matrine and oxymatrine from Sophora flavescens. J. Southwest For. Coll. 26, 37–39. DOI: 1003-7179(2006)02-0037-03.
Search in Google Scholar
11. Yang, L.M., Zhou, L., Ran, X.Y., Zhang, W.G. & Wu, L. (2006). Separation of oxymatrine from Sophora flavescens by macroporous absorption resin. J. North West Sci. Tech. Univ. Agri. For. 34, 129–132. DOI: 10.3321/j.issn:1671-9387. 2006.11.028.
Search in Google Scholar
12. Pedruzzi, I., Borges da Silva, E.A. & Rodrigues, A.E. (2008). Selection of resins, equilibrium and sorption kinetics of lactobionic acid, fructose, lactose and sorbitol. Sep. Purif. Technol. 63, 600–611. DOI: 10.1016/j.seppur.2014.08.020.10.1016/j.seppur.2014.08.020434054325729320
Search in Google Scholar
13. Fu, Y.J., Zu, Y.G., Liu, W., Hou, C.L. & Chen, L.Y. (2007). Preparative separation of vitexin and isovitexin from Pigeonpea extracts with macroporous resins. J. Chromatogr. A. 1139, 206–213. DOI: 10.1016/j.chroma.2006.11.015.10.1016/j.chroma.2006.11.01517140590
Search in Google Scholar
14. Fu, B.Q., Liu, J., Li, H., Li, H., Lee, Frank S.C. & Wang, X.R. (2005). The application of macroporous resins in the separation of licorice flavonoids and glycyrrhizic acid. J. Chromatogr. A 1089, 18–24. DOI: 10.1016/j.chroma.2005.06.05110.1016/j.chroma.2005.06.05116130766
Search in Google Scholar
15. Tang, Z.G., Zhou, R.Q. & Duan, Z.T. (2001) Adsorption and desorption behaviour of taurine on macroporous adsorption resins. J. Chem. Technol. Biotechnol. 76, 752–756. DOI: 10.1002/jctb.446.10.1002/jctb.446
Search in Google Scholar
16. Wan, J.B., Zhang, Q.W., Ye, W.C. & Wang, Y.T. (2008). Quantification and separation of protopanaxatriol and protopanaxadilo type saponins from Panax notoginseng with macroporous resins. Sep. Purif. Technol. 60, 198–205. DOI: 10.1016/j.seppur.2014.08.020.10.1016/j.seppur.2014.08.020
Search in Google Scholar
17. Cren, Érika C., Cardozo Filho, L., Silva, Edson A. & Meirelles, Antonio J.A. (2009). Breakthrough curves for oleic acid removal from ethanolic solutions using a strong anion exchange resin. Sep. Purif. Technol. 69, 1–6. DOI: 10.1016/j.seppur.2014.08.020.10.1016/j.seppur.2014.08.020
Search in Google Scholar
18. Silva, E.M., Pompeu, D.R., Larondelle, Y. & Rogez, H. (2007). Optimisation of the adsorption of polyphenols from Inga edulis leaves on macroporous resins using an experimental design methodology. Sep. Purif. Technol. 53, 274–280. DOI: 10.1016/j.seppur.2014.08.020.10.1016/j.seppur.2014.08.020
Search in Google Scholar
19. Zhang, B., Yang, R.Y., Zhao, Y. & Liu, C.Z. (2008). Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins. J. Chromatogr. B. 867, 253–258. DOI: 10.1016/j.jchromb.2008.04.016.10.1016/j.jchromb.2008.04.016
Search in Google Scholar
20. Bernardin, F.E. (1985). In: F.L. Slejko (Eds.), Experimental design and testing of adsorption and adsorbates (1–35). Marcel Dekker, New York: Adsorption Technology.
Search in Google Scholar
21. Wang, F., Wang, L.J., Li, J.S., Sun, X.Y. & Han, W.Q. (2009). Adsorption behavior and mechanism of cadmium on strong-acid cation exchange resin. Trans. Nonferrous Met. Soc. China 19, 740–744. DOI: 10.1016/S1003-6326(08)60343-X.10.1016/S1003-6326(08)60343-X
Search in Google Scholar
22. Juang, R.S. & Shiau, J.Y. (1999). Adsorption isotherms of phenols from water onto macroreticular resins. J. Hazard. Mater. B. 70, 171–183. DOI: 10.1016/S0304-3894(99)00152-1.10.1016/S0304-3894(99)00152-1
Search in Google Scholar
23. Baskaralingam, P., Pulikesi, M., Elango, D., Ramamurthi, V. & Sivanesan, S. (2006). Adsorption of acid dye onto organobentonite. J. Hazard. Mater. B. 128, 138–144. DOI: 10.1016/j.jhazmat.2005.07.049.10.1016/j.jhazmat.2005.07.04916360263
Search in Google Scholar
24. Langmuir, I. (1918). The adsorption of gases on plane surfaces of glass, mica and platinum. J. Am. Chem. Soc. 40, 1361–1402. DOI: 10.1021/Ja02242a004.10.1021/ja02242a004
Search in Google Scholar
25. Freundlich, H. (1909). The theory of adsorption. Z. Chem. Ind. Kolloide 3, 212–220. DOI: 10.1007/Bf01427691.10.1007/BF01427691
Search in Google Scholar
26. Treybal, R.E. (1981). Mass-Transfer Operations (3rd ed.). Singapore: McGraw-Hill International Companies.
Search in Google Scholar

Separation of matrine and oxymatrine from Sophora flavescens extract through cation exchange resin coupled with macroporous absorption resin

Abstract

Paradigm

My account