Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing

Muqiu Zhao; Hui Wang; Shuai Wang; Qiuying Han; Yunfeng Shi

doi:10.1515/ohs-2020-0036

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Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing

Oceanological and Hydrobiological Studies

Volume 49 (2020): Issue 4 (December 2020)

By: Muqiu Zhao, Hui Wang, Shuai Wang, Qiuying Han and Yunfeng Shi

Open Access

|Nov 2020

Figures & Tables

Location of five sampling sites with four monospecific meadows and the bare tidal flat at the intertidal zone of Xincun Bay

Solution ³¹P-NMR spectra for NaOH-EDTA extracts of the sediments from different seagrass meadows

Proportion of P forms in solution ³¹P-NMR spectra for NAOH-EDTA

NMDS plots of the sediment bacterial (A) and fungal OTUs (B) based on the unweighted UniFrac metric for all samples

Table 1

The number of operational taxonomic units (OTUs) and Alpha diversity indices of bacterial and fungal communities. The values are means followed by a standard error. Different letters indicate statistically significant differences (p < 0.05) according to the Duncan test.

	Samples	OTUs	Shannon Index	Simpson Index	Ace Index	Chao Index
	E. acoroides	1832 (124)^a	5.72 (0.24)^a	0.012 (0.004)^a	2147 (104)^a	2142 (99)^a
	T. hemprichii	2091 (6)^b	5.88 (0.22)^a	0.011 (0.005)^a	2426 (53)^b	2428 (59)^b
	C. rotundata	2081 (54)^b	5.72 (0.39)^a	0.019 (0.015)^a	2424 (15)^b	2414 (48)^b
Bacteria	H. ovalis	2105 (103)^b	6.08 (0.11)^a	0.007 (0.001)^a	2392 (83)^b	2408 (95)^b
	bare tidal flat	1937 (15)^a	5.93 (0.07)^a	0.009 (0.001)^a	2313 (38)^b	2316 (63)^b
	F test	7.427	1.233	1.058	9.295	7.555
	p value	0.005	0.357	0.426	0.002	0.005
	E. acoroides	299 (63)^b	3.91 (0.45)^b	0.064 (0.033)^a	303 (65)^b	303 (65)^b
	T. hemprichii	219 (40)^ab	2.82(0.48)^a	0.188 (0.093)^b	226 (41)^ab	231 (43)^ab
	C. rotundata	207 (58)^a	4.12 (0.41)^b	0.040 (0.023)^a	211 (58)^a	210 (57)^a
Fungi	H. ovalis	251 (22)^ab	3.87 (0.19)^b	0.071 (0.007)^a	256 (21)^ab	259 (26)^ab
	bare tidal flat	241 (27)^ab	4.37 (0.03)^b	0.029 (0.002)^a	246 (28)^ab	246 (29)^ab
	F test	1.907	8.451	5.796	1.810	1.694
	p value	0.186	0.003	0.011	0.203	0.227

Relative abundance of bacterial (A) and fungal (B) phyla

Relative abundance of the top five genus-level P-cycling-related bacteria (A) and fungi (B) detected by ³¹P-NMR. Blue arrows represent different genera of microbes, while red arrows represent P forms. Correlation between P forms and RDA axes is shown by both the length and angle of the arrows.

Redundancy analysis (RDA) for the top five genus-level P-cycling-related microbes (A for bacteria and B for fungi) and sediment P forms detected by ³¹P-NMR. The blue arrows represent different genera of microbes, whereas variables for P forms are shown with red arrows. The correlation between P forms and RDA axes are shown by both the length and angle of the arrows.

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DOI: https://doi.org/10.1515/ohs-2020-0036 | Journal eISSN: 1897-3191 | Journal ISSN: 1730-413X

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Language: English

Page range: 408 - 420

Submitted on: Apr 10, 2020

Accepted on: May 25, 2020

Published on: Nov 26, 2020

Published by: University of Gdańsk

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

phosphorus forms,

microbial community,

solution ³¹P-NMR,

high-throughput sequencing,

tropical seagrass sediments

Related subjects:

© 2020 Muqiu Zhao, Hui Wang, Shuai Wang, Qiuying Han, Yunfeng Shi, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 49 (2020): Issue 4 (December 2020)

Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing

Figures & Tables

Figure 1

Figure 2

Figure 3

Figure 4

Table 1

Figure 5

Figure 6

Figure 7

Figure 8

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