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Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing Cover

Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing

Open Access
|Nov 2020

Figures & Tables

Figure 1

Location of five sampling sites with four monospecific meadows and the bare tidal flat at the intertidal zone of Xincun Bay
Location of five sampling sites with four monospecific meadows and the bare tidal flat at the intertidal zone of Xincun Bay

Figure 2

Solution 31P-NMR spectra for NaOH-EDTA extracts of the sediments from different seagrass meadows
Solution 31P-NMR spectra for NaOH-EDTA extracts of the sediments from different seagrass meadows

Figure 3

Proportion of P forms in solution 31P-NMR spectra for NAOH-EDTA
Proportion of P forms in solution 31P-NMR spectra for NAOH-EDTA

Figure 4

NMDS plots of the sediment bacterial (A) and fungal OTUs (B) based on the unweighted UniFrac metric for all samples
NMDS plots of the sediment bacterial (A) and fungal OTUs (B) based on the unweighted UniFrac metric for all samples

Figure 5

Relative abundance of bacterial (A) and fungal (B) phyla
Relative abundance of bacterial (A) and fungal (B) phyla

Figure 6

Relative abundance of the top five genus-level P-cycling-related bacteria (A) and fungi (B) detected by 31P-NMR. Blue arrows represent different genera of microbes, while red arrows represent P forms. Correlation between P forms and RDA axes is shown by both the length and angle of the arrows.
Relative abundance of the top five genus-level P-cycling-related bacteria (A) and fungi (B) detected by 31P-NMR. Blue arrows represent different genera of microbes, while red arrows represent P forms. Correlation between P forms and RDA axes is shown by both the length and angle of the arrows.

Figure 7

Redundancy analysis (RDA) for the top five genus-level P-cycling-related microbes (A for bacteria and B for fungi) and sediment P forms detected by 31P-NMR. The blue arrows represent different genera of microbes, whereas variables for P forms are shown with red arrows. The correlation between P forms and RDA axes are shown by both the length and angle of the arrows.
Redundancy analysis (RDA) for the top five genus-level P-cycling-related microbes (A for bacteria and B for fungi) and sediment P forms detected by 31P-NMR. The blue arrows represent different genera of microbes, whereas variables for P forms are shown with red arrows. The correlation between P forms and RDA axes are shown by both the length and angle of the arrows.

Figure 8

Main findings of this research
Main findings of this research

The number of operational taxonomic units (OTUs) and Alpha diversity indices of bacterial and fungal communities_ The values are means followed by a standard error_ Different letters indicate statistically significant differences (p < 0_05) according to the Duncan test_

SamplesOTUsShannon IndexSimpson IndexAce IndexChao Index
E. acoroides1832 (124)a5.72 (0.24)a0.012 (0.004)a2147 (104)a2142 (99)a
T. hemprichii2091 (6)b5.88 (0.22)a0.011 (0.005)a2426 (53)b2428 (59)b
C. rotundata2081 (54)b5.72 (0.39)a0.019 (0.015)a2424 (15)b2414 (48)b
BacteriaH. ovalis2105 (103)b6.08 (0.11)a0.007 (0.001)a2392 (83)b2408 (95)b
bare tidal flat1937 (15)a5.93 (0.07)a0.009 (0.001)a2313 (38)b2316 (63)b
F test7.4271.2331.0589.2957.555
p value0.0050.3570.4260.0020.005
E. acoroides299 (63)b3.91 (0.45)b0.064 (0.033)a303 (65)b303 (65)b
T. hemprichii219 (40)ab2.82(0.48)a0.188 (0.093)b226 (41)ab231 (43)ab
C. rotundata207 (58)a4.12 (0.41)b0.040 (0.023)a211 (58)a210 (57)a
FungiH. ovalis251 (22)ab3.87 (0.19)b0.071 (0.007)a256 (21)ab259 (26)ab
bare tidal flat241 (27)ab4.37 (0.03)b0.029 (0.002)a246 (28)ab246 (29)ab
F test1.9078.4515.7961.8101.694
p value0.1860.0030.0110.2030.227
DOI: https://doi.org/10.1515/ohs-2020-0036 | Journal eISSN: 1897-3191 | Journal ISSN: 1730-413X
Language: English
Page range: 408 - 420
Submitted on: Apr 10, 2020
Accepted on: May 25, 2020
Published on: Nov 26, 2020
Published by: University of Gdańsk
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2020 Muqiu Zhao, Hui Wang, Shuai Wang, Qiuying Han, Yunfeng Shi, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.