Figure 1
Figure 2
Volumetric lipid productivity (PLV) of green algae reported in literature, listed together with Baltic strains indicated in this study as characterized by high productivity
| Species/strain | PLV [mg × l−1 × day−1] | Culture conditions temp.; light; medium | References |
|---|---|---|---|
| Botryococcus braunii | 5.51 | 25°C; 150 μmol m2 s−1; CHU13 | (Yoo et al. 2010) |
| Monoraphidium sp. FXY-10 | 6.88 | 25°C; 70 μmol m2 s−1; BG11 | (Yu et al. 2012) |
| Chlorella vulgaris | 6.91 | 25°C; 150 μmol m2 s−1; BG11 | (Yoo et al. 2010) |
| Scenedesmus sp. | 9.50 | 150 μmol m2 s−1 | (Lee et al. 2010) |
| Chlorella minutussima | 10.24 (calculated) | 25°C; 77 μmol m2 s−1; Guillard’s marine medium | (Illman et al. 2000) |
| Chlorella vulgaris | 11.10 | 150 μmol m2 s−1; BG11 | (Lee et al. 2010) |
| Chlorococcum sp. | 11.30 | 28°C; 54 μmol m2 s−1; BG11 | (Harwati et al. 2012) |
| Botryococcus sp. | 11.50 | 150 μmol m2 s−1; BG11 | (Lee et al. 2010) |
| Microthamnion sp. BA-173 | 17.23 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Oocystis submarina BA-1 | 19.92 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Scenedemus sp. | 20.65 | 25°C; 140 μmol m2 s−1; CHU13; CO2 enriched | (Yoo et al. 2010) |
| Chlorella vulgaris BA-2 | 23.89 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Kirchneriella lunaris | 24.22 | 25°C; 140 μmol m2 s−1; CHU13; CO2 enriched | (Nascimento et al. 2013) |
| Scenedesmus obliquus | 26.77 | 25°C; 140 μmol m2 s−1; CHU13; CO2 enriched | (Nascimento et al. 2013) |
| Chlorella vulgaris | 27.00 | 25°C; 250 μmol m2 s−1; 3N BBM; CO2 enriched | (Griffiths et al. 2012) |
| Monoraphidium sp. BA-165 | 28.85 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Scenedesmus sp. | 29.00 | 25°C; 250 μmol m2 s−1; 3N BBM; CO2 enriched | (Griffiths et al. 2012) |
| Chlorella fusca BA-18 | 29.49 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Chlorella minutussima BA-12 | 31.23 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Chlorella sp. BA-167 | 31.26 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Chlorella vulgaris BA-80 | 37.43 | 18°C; 100 μmol m2 s−1; f/2 | this study |
| Ankistrodesmus falcatus | 56.07 | 25°C; 140 μmol m2 s−1; CHU13; CO2 enriched | (Nascimento et al. 2013) |
| Botryococcus braunii | 112.43 | 25°C; 140 μmol m2 s−1; CHU13; CO2 enriched | (Nascimento et al. 2013) |
| Chlorella vulgaris | 204.91 | 25°C; 140 μmol m2 s−1; CHU13; CO2 enriched | (Nascimento et al. 2013) |
List of strains used in the study and their morphological characteristics_ Species names are consistent with the catalog of the CCBA collection_
| Strain | CCBA designation | Cell volume min.- (median)-max [μm3] | Cell shape |
|---|---|---|---|
| Oocystis submarina | BA-1 | 42–(92)–285 | rotational ellipsoid |
| Chlorella vulgaris | BA-2 | 65–(133)–303 | sphere |
| Oocystis parva | BA-3 | 45–(147)–260 | rotational ellipsoid |
| Chlorella minutussima | BA-12 | 8–(18)–73 | sphere |
| Chlorella fusca | BA-18 | 33–(113)–2144 | sphere |
| Chlorella vulgaris | BA-76 | 15–(35)–103 | rotational ellipsoid |
| Chlorella vulgaris | BA-80 | 65–(155)–425 | sphere |
| Scenedesmus sp. | BA-147 | 66–(244)–707 | rotational ellipsoid |
| Dictyosphaerium sp. | BA-157 | 31–(131)–298 | sphere |
| Monoraphidium sp. | BA-165 | 34–(67)–120 | 2 cones |
| Chlorella sp. | BA-167 | 19–(50)–126 | sphere |
| Stichococcus sp. | BA-168 | 5–(12)–24 | cylinder |
| Oocystis cf. submarina | BA-172 | 66–(129)–388 | rotational ellipsoid |
| Microthamnion kuetzingianum | BA-173 | 48–(131)–237 | cylinder; filamentous |
Growth and lipid accumulation characteristics in the tested strains
| Strain | Final cell count [cells ml−1] | Growth rate μ [d−1] | Lipid yield [mg l−1] | Cellular lipid content [pg cell−1] |
|---|---|---|---|---|
| Oocystis submarina BA-1 | 3.56 × 106 ± 7.72 × 104 | 0.35 ± 0.01 | 57.59 ± 0.50 | 16.19 ± 0.29 |
| Chlorella vulgaris BA-2 | 1.13 × 107±2.66 × 105 | 0.42 ± 0.01 | 56.82 ± 6.29 | 5.05 ± 0.43 |
| Oocystis parva BA-3 | 7.25 × 106 ±1.53 × 105 | 0.38 ± 0.01 | 38.64 ± 2.17 | 5.33 ± 0.33 |
| Chlorella minutussima BA-12 | 3.72 × 107 ± 7.25 × 106 | 0.5 ± 0.06 | 62.52 ± 9.78 | 1.68 ± 0.26 |
| Chlorella fusca BA-18 | 1.64 × 107 ± 3.85 × 105 | 0.45 ± 0.01 | 64.85 ± 10.81 | 3.96 ± 0.64 |
| Chlorella vulgaris BA-76 | 1.33 × 106 ± 7.83 × 105 | 0.26 ± 0.03 | 3.50 ± 3.13 | 2.63 ± 2.51 |
| Chlorella vulgaris BA-80 | 3.07 × 107 ±1.44 × 106 | 0.49 ± 0.01 | 75.90 ± 9.32 | 2.48 ± 0.41 |
| Scenedesmus sp. BA-147 | 1.87 × 106 ±2.02 × 105 | 0.26 ± 0.01 | 46.39 ± 8.49 | 24.81 ± 2.70 |
| Dictyosphaerium sp. BA-157 | 9.84 × 106 ± 3.71 × 105 | 0.30 ± 0.01 | 31.57 ± 3.44 | 3.21 ±0.15 |
| Monoraphidium sp. BA-165 | 1.30 × 107 ± 3.65 × 105 | 0.35 ± 0.01 | 81.13 ± 15.91 | 6.26 ± 1.29 |
| Chlorella sp. BA-167 | 1.65 × 107 ± 2.64 × 105 | 0.39 ± 0.01 | 80.53 ± 3.60 | 4.89 ±0.13 |
| Stichococcus sp. BA-168 | 1.95 × 107 ± 3.38 × 106 | 0.35 ± 0.01 | 16.80 ± 4.34 | 0.86 ±0.15 |
| Oocystis cf. submarina BA-172 | 2.80 × 106 ± 9.05 × 104 | 0.24 ± 0.01 | 43.47 ± 7.29 | 15.54 ± 2.90 |
| Microthamnion sp. BA-173 | 4.61 × 106 ±1.70 × 105 | 0.30 ± 0.01 | 56.41 ± 2.74 | 12.23 ± 0.14 |