Have a personal or library account? Click to login
Characterization of Dinophysis acuminata from the Yellow Sea, China, and its response to different temperatures and Mesodinium prey Cover

Characterization of Dinophysis acuminata from the Yellow Sea, China, and its response to different temperatures and Mesodinium prey

Oceanological and Hydrobiological Studies
Volume 46 (2017): Issue 4 (December 2017)
By: Han Gao,  Xinlong An,  Lei Liu,  Ke Zhang,  Daoqiong Zheng and  Mengmeng Tong  
Open Access
|Dec 2017

Figures & Tables

Fig. 1

Light micrographs of cultured cells: (a) Dinophysis acuminata DAYS01, from Xiaoping Island, the Yellow Sea, China; (b)Mesodinium rubrum JAMR, from Japan; (c) Mesodinium rubrum AND-A0711, from Southern Spain. Scale bars = 10 μm
Light micrographs of cultured cells: (a) Dinophysis acuminata DAYS01, from Xiaoping Island, the Yellow Sea, China; (b)Mesodinium rubrum JAMR, from Japan; (c) Mesodinium rubrum AND-A0711, from Southern Spain. Scale bars = 10 μm

Fig. 2

Neighbor-joining phylogenetic tree of dinoflagellates inferred from mitochondrial cox1. The corresponding GenBank accession number follows the name of each organism. Numbers at nodes are interior branch test values for 1000 replicates. The scale bar represents the number of substitutions per site.
Neighbor-joining phylogenetic tree of dinoflagellates inferred from mitochondrial cox1. The corresponding GenBank accession number follows the name of each organism. Numbers at nodes are interior branch test values for 1000 replicates. The scale bar represents the number of substitutions per site.

Fig. 3

(a, b) Growth responses of D. acuminata (solid lines) under multiple temperature (10, 15 and 20°C) and two strains of ciliates (a: JAMR b: AND-A0711, dash lines); (c, d) Toxin profiles of D. acuminata (DAYS01) fed with M. rubrum from Japan (JAMR) and Spain (AND-A0711) under multiple temperatures (10, 15 and 20°C). Error bars: standard error
(a, b) Growth responses of D. acuminata (solid lines) under multiple temperature (10, 15 and 20°C) and two strains of ciliates (a: JAMR b: AND-A0711, dash lines); (c, d) Toxin profiles of D. acuminata (DAYS01) fed with M. rubrum from Japan (JAMR) and Spain (AND-A0711) under multiple temperatures (10, 15 and 20°C). Error bars: standard error

Fig. 4

LC-MS/MS chromatograms of OA, DTX1 (a) and PTX2 (b) in Dinophysis acuminata (DAYS01) cultures under 10, 15 and 20°C
LC-MS/MS chromatograms of OA, DTX1 (a) and PTX2 (b) in Dinophysis acuminata (DAYS01) cultures under 10, 15 and 20°C

Growth rates and maximum yield in cultures of Dinophysis acuminata isolated from the Yellow Sea, China, at three temperatures, when fed on ciliates Mesodinium rubrum from Japan (JAMR) and Spain (AND-A0711) (mean ± standard error, n = 2)

Temp. (°C)JAMRAND-A0711
Growth rate (d-1)Max yield (cells ml-1)Growth rate (d-1)Max yield (cells ml-1)
100.09 ± 0.03 (Day 1-Day9)

Time indicates the exponential growth period of each treatment

112 ± 270.09 ± 0.01 (Day 1-Day9)120 ± 3
150.21 ± 0.015 (Day 1-Day18)0.10 ± 0.001 (Day 1-Day21)2483 ± 46360 ± 52
200.29 ± 0.008 (Day 1-Day18)0.22 ± 0.004 (Day 1-Day15)7150 ± 2701343 ± 123
DOI: https://doi.org/10.1515/ohs-2017-0043 | Journal eISSN: 1897-3191 | Journal ISSN: 1730-413X
Journal RSS Feed
Language: English
Page range: 439 - 450
Submitted on: Mar 7, 2017
Accepted on: May 23, 2017
Published on: Dec 12, 2017
Published by: University of Gdańsk
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Dinophysis,
Diarrheic Shellfish,
Poisoning (DSP),
pectenotoxins (PTXs),
okadaic acid (OA),
dinophysistoxins (DTXs)
Related subjects:
Chemistry,
Chemistry, other,
Geosciences,
Geosciences, other,
Life sciences,
Life sciences, other

© 2017 Han Gao, Xinlong An, Lei Liu, Ke Zhang, Daoqiong Zheng, Mengmeng Tong, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 46 (2017): Issue 4 (December 2017)Next article