Molecular and ultrastructure characterization of two nematodes (Thelandros scleratus
and Physalopteroides dactyluris) based on ribosomal and mitochondrial DNA sequences

U Goswami; A Chaudhary; C Verma; H.S. Singh

doi:10.1515/helmin-2016-0013

Abstract

The phylogenetic relationships of the nematode species Thelandros scleratus (Oxyurida: Pharyn-godonidae) and Physalopteroides dactyluris (Spirurida: Physalopteridae) were analyzed using the ribosomal 18S rRNAand the mitochondrial cytochrome C oxidase subunit genes. The nematodes were recovered from Brook's house gecko, Hemidactylus brooki (Reptilia: Gekkonidae) from Hast-inapur, Meerut (U.P.), India. The results demonstrated that T. scleratus shows 100% similarity with another sequence available from the same species and a close relationship (98-99%) with species of Parapharyngodon in both 18S rRNAand cox 1 regions. Regarding the nematode Physalopteroides. analysis showed a close phylogenetic relationship between P. dactyluris and several species of Phy-saloptera. This is the first sequence of 18S available for any species of the genus Physalopteroides

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Molecular and ultrastructure characterization of two nematodes (Thelandros scleratus and Physalopteroides dactyluris) based on ribosomal and mitochondrial DNA sequences

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