Identification of Bacillus spp. colonizing the nasal mucosa of healthy adults living in the suburban area using the matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system

Urszula Kosikowska; Dagmara Stepien-Pysniak; Dorota Ozga; Andrzej Wernicki; Anna Malm

doi:10.1515/cipms-2015-0001

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Identification of Bacillus spp. colonizing the nasal mucosa of healthy adults living in the suburban area using the matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system

Current Issues in Pharmacy and Medical Sciences

Volume 27 (2014): Issue 3 (September 2014)

By: Urszula Kosikowska, Dagmara Stepien-Pysniak, Dorota Ozga, Andrzej Wernicki and Anna Malm

Open Access

|Mar 2015

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Abstract

Bacillus spp. can be regarded as a rare component of the nasal mucosa microflora. The aim of this study was to identify Bacillus spp. from the nasal mucosa of healthy adults living in the suburban area near Lublin using the matrix-assisted laser desorptionionization time-of-flight mass spectrometry (MALDI-TOF MS) system.

A total of 11 bacterial isolates from the nasal specimens were cultured. The following species were identified using the routine microbiological methods: Staphylococcus aureus (3 isolates), S. epidermidis (1 isolate), S. intermedius (1 isolate) and Staphylococcus spp. (1 isolate). Moreover, 2 strains of Escherichia coli were isolated. Besides, 3 isolates of Bacillus spp. were found. These isolates were characterized by means of MALDI-TOF MS, resulting in highly specific mass spectral fingerprints and these were identified as B. pumilus, B. safenis and B. licheniformis. It was observed that all studied Bacillus spp. isolates only had the masses in common at 3864 ± 2, 7727 ± 2, and 14301 ± 4. The spectra of B. safensis and B. pumilus showed peaks at m/z 4914 ± 3, 6621 ± 3 and 14291 ± 2, which were absent in the spectrum of B. licheniformis. For B. safenis and B. pumilus, other potential biomarkers could be found at m/z 12620 and 16668, respectively.

1. Alispahic M. et al.: Species-specific identification and differentiation of Arcobacter, Helicobacter and Campylobacter by full-spectral matrix-associated laser desorption/ionization time of flight mass spectrometry analysis. J. Med. Microbiol., 59, 295, 2010.
Search in Google Scholar
2. Ball S.C., Sepkowitz K.: Infection due to Bacillus cereus in an injection drug user with AIDS: bacteremia without morbidity. Clin. Infect. Dis., 19, 216, 1994.
Search in Google Scholar
3. Barrie D. et al.: Contamination of hospital linen by Bacillus cereus. Epidemiol. Infect., 113, 297, 1994.10.1017/S0950268800051724
Open DOI Search in Google Scholar
4. Bernardo K. et al.: Identification and discrimination of Staphylococcus aureus strains using matrix-assisted laser desorption/ ionization-time of flight mass spectrometry. Proteomics, 2, 747, 2002.10.1002/1615-9861(200206)2:6<;747::AID-PROT747>3.0.CO;2-V
Open DOI Search in Google Scholar
5. Blue S.R., Singh V.R., Saubolle M.A.: Bacillus licheniformis bacteremia: five cases associated with indwelling central venous catheters. Clin. Infect. Dis., 20, 629, 1995.
Search in Google Scholar
6. Böhme K. et al.: Species differentiation of seafood spoilage and pathogenic Gram-negative bacteria by MALDI-TOF mass fingerprinting. J. Proteome Res., 9, 3169, 2010.10.1021/pr100047q
Open DOI Search in Google Scholar
7. Bryce E.A. et al.: Dissemination of Bacillus cereus in an intensive care unit. Infect. Control Hosp. Epidemiol., 14, 459, 1993.10.1086/646779
Open DOI Search in Google Scholar
8. Clark A.E. et al.: Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clin. Microbiol. Rev., 26, 547, 2013.10.1128/CMR.00072-12
Open DOI Search in Google Scholar
9. Connor N. et al.: Ecology of speciation in the genus Bacillus. Appl. Environ. Microbiol., 76, 1349, 2010.10.1128/AEM.01988-09
Open DOI Search in Google Scholar
10. Cotton D.J. et al.: Clinical features and therapeutic interventions in 17 cases of Bacillus bacteremia in an immunosuppressed patient population. J. Clin. Microbiol. 25, 672, 1987.10.1128/jcm.25.4.672-674.1987
Open DOI Search in Google Scholar
11. Curtis J.R, Wing A.J., Coleman J.C.: Bacillus cereus bacteraemia - a complication of intermittent haemodialysis. Lancet, 1, 136, 1967.
Search in Google Scholar
12. Dickinson D.N. et al.: MALDI-TOF MS compared with other polyphasic taxonomy approaches for the identification and classification of Bacillus pumilus spores. J. Microbiol. Methods, 58, 1, 2004.10.1016/j.mimet.2004.02.011
Open DOI Search in Google Scholar
13. Dupont C. et al.: Identification of clinical coagulase-negative staphylococci, isolated in microbiology laboratories, by matrixassisted laser desorption/ionization-time of flight mass spectrometry and two automated systems. Clin. Microbiol. Infect., 16, 998, 2010.10.1111/j.1469-0691.2009.03036.x
Open DOI Search in Google Scholar
14. Farrar W.E.: Serious infections due to “non-pathogenic” organisms of the genus Bacillus. Am. J. Med., 34, 134, 1963.10.1016/0002-9343(63)90047-0
Open DOI Search in Google Scholar
15. Freiwald A., Sauer S.: Phylogenetic classification and identification of bacteria by mass spectrometry. Nat. Protoc., 4, 732, 2009.
Search in Google Scholar
16. Hotta Y. et al.: Classification of the genus Bacillus based on MALDITOF MS analysis of ribosomal proteins coded in S10 and spc operons. J. Agric. Food Chem., 59, 5222, 2011.10.1021/jf2004095
Open DOI Search in Google Scholar
17. Hsueh P.R. et al.: Nosocomial pseudo epidemic caused by Bacillus cereus traced to contaminated ethyl alcohol from a liquor factory. J. Clin. Microbiol., 37, 2280, 1999.10.1128/JCM.37.7.2280-2284.1999
Open DOI Search in Google Scholar
18. Ihde D.C., Armstrong D.: Clinical spectrum of infection due to Bacillus species. Am. J. Med., 55, 839, 1973.
Search in Google Scholar
19. Ivanova E.P., Mikhailov V.V., Andreev L.A.: Marine bacilli and some approaches to their identification. Mikrobiol. Zhurnal, 54, 27, 1922 (in Russian).
Search in Google Scholar
20. Knoester M. et al.: Routine identification of clinical isolates of anaerobic bacteria: matrix-assisted laser desorption ionizationtime of flight mass spectrometry performs better than conventional identification methods. J. Clin. Microbiol., 50, 1504, 2012.10.1128/JCM.06607-11
Open DOI Search in Google Scholar
21. Krishnamurthy T., Ross P.L., Rajamani U.: Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom., 10, 883, 1996.
Search in Google Scholar
22. Lavigne J.P. et al.: Mass spectrometry: a revolution in clinical microbiology? Clin. Chem. Lab. Med., 36, 1, 2012.
Search in Google Scholar
23. Lund B.M.: Foodborne, disease due to Bacillus and Clostridium species. Lancet, 336, 982, 1990.
Search in Google Scholar
24. Lynn E.C. et al.: Identification of Enterobacteriaceae bacteria by direct matrix-assisted laser desorptiom/ionization mass spectrometric analysis of whole cells. Rapid Commun. Mass Spectrom., 13, 2022, 1999.10.1002/(SICI)1097-0231(19991030)13:20<;2022::AID-RCM750>3.0.CO;2-3
Open DOI Search in Google Scholar
25. O’Day D.M. et al.: The problem of Bacillus infection with special emphasis on the virulence of Bacillus cereus. Ophthalmol., 88, 833, 1981.10.1016/S0161-6420(81)34960-4
Open DOI Search in Google Scholar
26. Oggioni M.R. et al.: Recurrent septicemia in an immunocompromised patient due to probiotic strains of Bacillus subtilis. J. Clin. Microbiol., 36, 325, 1998.10.1128/JCM.36.1.325-326.1998
Open DOI Search in Google Scholar
27. Ozkocaman V. et al. Bacillus spp. among hospitalized patients with haematological malignancies: clinical features, epidemics and outcomes. J. Hosp. Infect., 64, 169, 2006.10.1016/j.jhin.2006.05.014
Open DOI Search in Google Scholar
28. Pribil P., Fenselau C.: Characterization of enterobacteria using MALDI-TOF mass spectrometry. Anal. Chem., 77, 6092, 2005.
Search in Google Scholar
29. Rajakaruna L. et al.: High throughput identification of clinical isolates of Staphylococcus aureus using MALDI-TOF MS of intact cells. Infect. Genet. Evol., 9, 507, 2009.10.1016/j.meegid.2009.01.012
Open DOI Search in Google Scholar
30. Satomi M., La Duc M.T., Venkateswaran K.: Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces. Int. J. Syst. Evol. Microbiol., 56, 1735, 2006.
Search in Google Scholar
31. Sauer S. et al.: Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS ONE, 3, e2843, 2008. (doi: 10.1371/journal.pone. 0002843)
Search in Google Scholar
32. Sorokulova I.: Modern status and perspectives of Bacillus bacteria as probiotics. J. Prob. Health., 1, 1000e106, 2013. (doi: 10.4172/2329-8901.1000e106)
Search in Google Scholar
33. Tuazon C.U. et al.: Serious infections from Bacillus sp. JAMA, 241, 1137, 1979.10.1001/jama.1979.03290370041026
Open DOI Search in Google Scholar
34. Van Veen S., Claas E., Kuijper E.J.: High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization- time of f light mass spectrometry in conventional medical microbiology laboratories. J. Clin. Microbiol., 48, 900, 2010.
Search in Google Scholar
35. Wolters M. et al.: MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages. Int. J. Med. Microbiol., 301, 64, 2011. 10.1016/j.ijmm.2010.06.002
Open DOI Search in Google Scholar

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DOI: https://doi.org/10.1515/cipms-2015-0001 | Journal eISSN: 2300-6676 | Journal ISSN: 2084-980X

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Language: English

Page range: 137 - 141

Submitted on: Oct 1, 2014

Accepted on: Oct 20, 2014

Published online: Mar 3, 2015

Published in print: Sep 1, 2014

Published by: Sciendo

In partnership with: Paradigm Publishing Services

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Pharmacology, toxicology

© 2015 Urszula Kosikowska, Dagmara Stepien-Pysniak, Dorota Ozga, Andrzej Wernicki, Anna Malm, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 27 (2014): Issue 3 (September 2014)