Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

Kamila Rokicka; Magdalena Wojciak-Kosior

doi:10.1515/cipms-2015-0002

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Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

Current Issues in Pharmacy and Medical Sciences

Volume 27 (2014): Issue 3 (September 2014)

By: Kamila Rokicka and Magdalena Wojciak-Kosior

Open Access

|Mar 2015

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Abstract

In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.

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Articles in this issue

DOI: https://doi.org/10.1515/cipms-2015-0002 | Journal eISSN: 2300-6676 | Journal ISSN: 2084-980X

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Language: English

Page range: 142 - 144

Submitted on: Aug 20, 2014

Accepted on: Aug 26, 2014

Published online: Mar 3, 2015

Published in print: Sep 1, 2014

Published by: Sciendo

In partnership with: Paradigm Publishing Services

Keywords:

amyrin,

Related subjects:

Pharmacology, toxicology

© 2015 Kamila Rokicka, Magdalena Wojciak-Kosior, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 27 (2014): Issue 3 (September 2014)