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Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models Cover

Protective effect of Helicteres isora, an efficient candidate on hepatorenal toxicity and management of diabetes in animal models

Open Chemistry
Volume 23 (2025): Issue 1 (January 2025)
By: Hayat Bilal,  Mubarak Ali Khan,  Syed Wadood Ali Shah,  Mehreen Ghias,  Adnan Khan,  Muhammad Zahoor,  Riaz Ullah,  Zafar Iqbal and  Abdul Bari Shah  
Open Access
|Jun 2025

Figures & Tables

Figure 1

GCMS results and phytochemical constituents of H. isora.
GCMS results and phytochemical constituents of H. isora.

Figure 2

Determination of blood glucose levels. Data are expressed as mean ± SEM with n = 8. One-way ANOVA and Dunnett’s post-hoc test were used to determine statistical significance. !!!P < 0.001 was considered significant compared to the control group. Within the diabetic group, differences were noted with significance levels of *P < 0.05, **P < 0.01, and ***P < 0.001.
Determination of blood glucose levels. Data are expressed as mean ± SEM with n = 8. One-way ANOVA and Dunnett’s post-hoc test were used to determine statistical significance. !!!P < 0.001 was considered significant compared to the control group. Within the diabetic group, differences were noted with significance levels of *P < 0.05, **P < 0.01, and ***P < 0.001.

Figure 3

(a) Effect of Hi-Crd and fractions on the weight of animals. (b) Weight of pancreas. Data are expressed as mean ± SEM with n = 8. One-way ANOVA and Dunnett’s post-hoc test were used to determine statistical significance. !!!P < 0.001 was considered significant compared to the control group. Within the diabetic group, differences were noted with significance levels of *P < 0.05, **P < 0.01, and ***P < 0.001.
(a) Effect of Hi-Crd and fractions on the weight of animals. (b) Weight of pancreas. Data are expressed as mean ± SEM with n = 8. One-way ANOVA and Dunnett’s post-hoc test were used to determine statistical significance. !!!P < 0.001 was considered significant compared to the control group. Within the diabetic group, differences were noted with significance levels of *P < 0.05, **P < 0.01, and ***P < 0.001.

Figure 4

(a) Total cholesterol and triglycerides levels of diabetic animals. (b) HDL and LDL levels of diabetic animals. Anti-hyperlipidemic activity of H. isora is shown. Data are expressed as mean ± SEM with n = 8. One-way ANOVA and Dunnett’s post-hoc test were used to determine statistical significance. !!!P < 0.001 was considered significant compared to the control group. Within the diabetic group, differences were noted with significance levels of *P < 0.05, **P < 0.01, and ***P < 0.001.
(a) Total cholesterol and triglycerides levels of diabetic animals. (b) HDL and LDL levels of diabetic animals. Anti-hyperlipidemic activity of H. isora is shown. Data are expressed as mean ± SEM with n = 8. One-way ANOVA and Dunnett’s post-hoc test were used to determine statistical significance. !!!P < 0.001 was considered significant compared to the control group. Within the diabetic group, differences were noted with significance levels of *P < 0.05, **P < 0.01, and ***P < 0.001.

Figure 5

Protective effect of H. isora on paracetamol-induced toxicity in kidneys. (a) Normal; (b) Standard; (c) Paracetamol; (d) Chl 150 mg/kg body weight and (e) Chl 300 mg/kg body weight.
Protective effect of H. isora on paracetamol-induced toxicity in kidneys. (a) Normal; (b) Standard; (c) Paracetamol; (d) Chl 150 mg/kg body weight and (e) Chl 300 mg/kg body weight.

Figure 6

Protective effect of H. isora on paracetamol-induced toxicity in the liver. (a) Normal; (b) Standard; (c) Paracetamol; (d) Chl 150 mg/kg body weight and (e) Chl 300 mg/kg body weight.
Protective effect of H. isora on paracetamol-induced toxicity in the liver. (a) Normal; (b) Standard; (c) Paracetamol; (d) Chl 150 mg/kg body weight and (e) Chl 300 mg/kg body weight.

Effect of H_ isora extracts on the α-glucosidase enzyme inhibition activity with IC50 values

Sample α-Glucosidase inhibitory activity with IC50 values(µg/mL)
Acarbose12.54
Hi-Crd140.34
Hi-Chl122.63
Hi-Et220.26

Biochemical analysis of the kidney function test

Biochemical analysis Serum creatinine (mg/dl)Blood urea (mg/dl)Uric acid (mg/dl)
Normal0.43 ± 0.0513.60 ± 0.661.32 ± 0.21
Paracetamol0.91 ± 0.0841.30 ± 2.014.54 ± 0.25
Silymarin 40 mg/kg0.45 ± 0.05***18.10 ± 1.19***2.07 ± 0.25***
Hi-Crd 1500.57 ± 0.06*29.30 ± 2.72***3.03 ± 0.3**
Hi-Crd 3000.51 ± 0.04***21.60 ± 2.17***2.71 ± 0.12***
Hi-Chl 750.55 ± 0.11**23.30 ± 2.10***2.82 ± 0.26***
Hi-Chl 1500.48 ± 0.07***19.64 ± 1.22***2.23 ± 0.21***
Hi-Et 750.62 ± 0.07*31.00 ± 1.57**3.38 ± 0.24*
Hi-Et 1500.57 ± 0.08**28.33 ± 1.35***3.40 ± 0.10**

Biochemical analysis of bilirubin, SGPT, and ALP for liver function tests

Biochemical analysis Bilirubin (mg/dl)SGPT (U/l)ALP
Normal0.21 ± 0.0467.10 ± 2.92157.10 ± 3.09
Paracetamol0.73 ± 0.03118.50 ± 3.96207.50 ± 3.34
Silymarin 40 mg/kg0.24 ± 0.07***73.30 ± 2. 44***169.10 ± 2.86***
Hi-Crd 1500.35 ± 0.05***101.07 ± 2.38**191.60 ± 2.41**
Hi-Crd 3000.30 ± 0.04***88.50 ± 2.10***182.60 ± 2.89***
Hi-Chl 750.32 ± 0.07***82.30 ± 2.51***173.30 ± 2.07***
Hi-Chl 1500.26 ± 0.06***73.10 ± 4.40***174.60 ± 2.81***
Hi-Et 750.36 ± 0.07**106.10 ± 3.56*193.60 ± 2.81**
Hi-Et 1500.32 ± 0.04***94.10 ± 2.911***188.50 ± 2.70***
DOI: https://doi.org/10.1515/chem-2025-0133 | Journal eISSN: 2391-5420
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Language: English
Submitted on: Jun 29, 2024
Accepted on: Feb 7, 2025
Published on: Jun 17, 2025
Published by: Sciendo
In partnership with: Paradigm Publishing Services
Keywords:
Helicteres isora ,
hepatotoxicity,
nephrotoxicity,
diabetes,
serum profiling,
biochemical analysis
Related subjects:
Chemistry,
Inorganic chemistry,
Chemistry,
Organic chemistry,
Chemistry,
Chemistry, other

© 2025 Hayat Bilal, Mubarak Ali Khan, Syed Wadood Ali Shah, Mehreen Ghias, Adnan Khan, Muhammad Zahoor, Riaz Ullah, Zafar Iqbal, Abdul Bari Shah, published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 License.

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